LOCUS       sequence-485693-       11221 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses QF2 in the fibroblasts of zebrafish along with the cardiac
            cmlc2:EGFP transgenesis marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11221)
  AUTHORS   Fazekas L, Kaszas D, Vamosi B, Tamas SX, Szollosi T, Mihalyi V, 
            Dehne FG, Vago-Kiss K, Al-Sheraji NM, Paulovits B, Roux BT, Enyedi B
  TITLE     Fibroblasts promote osmotic surveillance by wound-induced unique 
            calcium patterns.
  JOURNAL   J Cell Biol. 2026 Jan 5;225(1):e202501165. doi: 
            10.1083/jcb.202501165. Epub 2025 Oct 22.
  PUBMED    41123438
REFERENCE   2  (bases 1 to 11221)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2026-01-5"; volume: "225(1):e202501165. doi"; pages: 
            " 10.1083/jcb.202501165. Epub 2025 Oct 22"
COMMENT     Sequence Label: pDestTol2CG2-itga11a-cFos:QF2
FEATURES             Location/Qualifiers
     source          1..11221
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(21..37)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    53..187
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(489..1202,1203..1205,1206..1208))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(492..1202,1203..1205,1206..1208))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(534..555)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     883..902
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1142..1163
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    join(2431..2618,2619..2630)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
     primer_bind     complement(2664..2680)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(2717..2735)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2756..2772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2769..2791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2780..2796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2804..2810,2811..2828,2829..2834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2849..2870
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2987..3004)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3158..3746)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3238..3257)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3917..4708,4709..4777))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4540..4559
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4778..4882)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(5045..5066)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     misc_feature    join(5519..5530,5531..5693)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
     primer_bind     6066..6082
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6102..6122
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        6124..6142
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9358..9374)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(9553..9564)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        complement(9639..9657)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     protein_bind    9657..9681
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(10770..10794)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    10796..10930
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    11017..11151
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(join(11214..11221,1..13))
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 ttatacatag ttgataattc actggccgtc gttttacggt accatcgatg atgatccaga
       61 catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg
      121 ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa
      181 acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga
      241 ggttttttaa agcaagtaaa acctctacaa atgtggtatg gctgattatg atcctctaga
      301 tcagatctct tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
      361 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
      421 gtatcttatc atgtctggat ctacgtaata cgactcacta tagttctaga ggctcgagag
      481 gggccgcttt acttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac
      541 tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagggc ggactgggtg
      601 ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg
      661 tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc
      721 ttgatgccgt tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac
      781 tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg
      841 cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg
      901 tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag
      961 tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg
     1021 gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc
     1081 agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt
     1141 acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg
     1201 ctcaccatgg tcactgtctg ctttgctgtt ggtctgggct cctgggtcac tggcttacta
     1261 atggagtctt tatgtatgag gactcttatc aattgttctt ctataaaggt ctgcagtgtt
     1321 tctgttcgtc ccctacatgg acacccagag cctcctaaat acaggagccc tgataactgc
     1381 acaagtgctc agattccagc agggtggaaa atgagataaa gtgtgcagat ggggaggggg
     1441 acgtgaatga gagatttgag ggatgaaaag gatggatgaa cgcattgaaa atagcccctt
     1501 tcacaaaata ataccagtaa attgtcatat aattaatgac cagaccttta cctgtaaatg
     1561 atgtgatgtt catatacaga agaatgccaa caatttacag atgattttac aaattcttaa
     1621 ttttcctttt ctaaatggat ttgttcacac attatctctt caaagcaatt gaattttctg
     1681 gaaaagattg gctgtgtgtg taaaaggtgc tactgaggaa tttaatgtga catggaatga
     1741 agcaaacagc agcctaagac aggatggcag gaaaaatgtc acatgtttaa aataagagtg
     1801 aaaccaacga cctggactta tagagactgt gtgtccttag gcaaagctct tgtacaaaca
     1861 gtgcttgtga ctgtgaaatt cggggtttgc ctggattgtg ttaaatgtgt gcttggatgt
     1921 atcataaaaa agaatcggtt ctctgtattt gacattttct gtaatttatc taaagttaca
     1981 actggctatg cctgattttt atcactattg aatcaatcat tttaaagaat gttaagtctg
     2041 ctgatttgga gagctcattt acatttattt tgaatgtctc ttatttaaca caactgattt
     2101 aagctttaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
     2161 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
     2221 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
     2281 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
     2341 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
     2401 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
     2461 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
     2521 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
     2581 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc
     2641 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga
     2701 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     2761 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     2821 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     2881 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     2941 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     3001 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     3061 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     3121 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     3181 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     3241 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     3301 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     3361 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     3421 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     3481 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     3541 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     3601 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     3661 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     3721 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     3781 ctcagtggaa cgaaaactca cgttaaggca ttttggtcat gagattatca aaaaggatct
     3841 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     3901 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     3961 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     4021 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     4081 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     4141 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     4201 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     4261 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     4321 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     4381 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
     4441 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
     4501 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
     4561 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
     4621 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
     4681 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
     4741 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
     4801 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
     4861 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctaaa ttgtaagcgt
     4921 taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     4981 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     5041 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     5101 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     5161 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     5221 ttgacgggga aaggctggct aagaactcat cagcctcccc ggtccatcta cccacgtacc
     5281 aatgcaccaa ttggccacaa tgacggctac tacatggtgc cattccttcc tctttatagg
     5341 aatggagact acctcctgtc caacaatgct cttggatacg agtacgccta cctgttggac
     5401 ccaggtcatt gcacaacacc agaaatgccc tctgatctgc aaaagacgtg aatatctgtt
     5461 cagacaccca tatccactct gttccacaca ggtcagaggt ttgtccagga gttcttgaca
     5521 gaggtgtaaa aagtactcaa aaattttact caagtgaaag tacaagtact tagggaaaat
     5581 tttactcaat taaaagtaaa agtatctggc tagaatctta cttgagtaaa agtaaaaaag
     5641 tactccatta aaattgtact tgagtattaa ggaagtaaaa gtaaaagcaa gaaagaaaac
     5701 tagagattct tgtttaagct tttaatctca aaaaacatta aatgaaatgc atacaaggtt
     5761 ttatcctgct ttagaactgt ttgtatttaa ttatcaaact ataagacaga caatctaatg
     5821 ccagtacacg ctactcaaag ttgtaaaacc tcagatttaa cttcagtaga agctgattct
     5881 caaaattgtt agtgtcaagc ctagctcttt tggggctgaa aagcaatcct gcagtgctga
     5941 aaagcctctc acaggcagcc gatgcgggaa gaggtgtatt agtcttgata gagaggctgc
     6001 aaatagcagg aaacgtgagc agagactccc tggtgtctga aacacaggcc agatgggccc
     6061 tcgagcagga aacagctatg accatgatta cgccaagcta tcaactttgt atagaaaagt
     6121 tggtaatacg actcactata gggcgaattg ggtaccgaat tgctctgctt tgatggaaat
     6181 atttgatgaa aggaaagaca gatgactttt taagtactct taatgaatgc agcgctcttt
     6241 actttggtca tttttgtttt aagcaaaatc agctcagtgt tgatcagatg tttggtctct
     6301 ttggtttcaa acttaaggca catacatagt agaaactgca acatcctcat actattaaga
     6361 atttatatat tctttattaa tcgtgtgttc aatagtatgt aacattgatt tttcaactca
     6421 gatcttcctc cacaatttgg tggattagtt ttctggcagt cagaaggtcg tgagttcttt
     6481 aatgtgcttc tttaatgttt tattgtttat ctataatatt gggtatcatg gattttgagt
     6541 atataatggt acaaataatg agataaaaat gtatttttat tgttttggac agtcggaatt
     6601 ccagacatga ataagtgaat gatgataatt gaatattatt ttcctataca atggagataa
     6661 tgcttttaaa ggtaaaggaa atatttaaat atctgtggtt aattacagta tagtaaaaac
     6721 gagtttttaa ttattaagtg gtcgtctaat taatgacata atgtgattga cttttgcaca
     6781 ttggaggaag gatgacacta tggctgaagt atttcctgac aggtaatgtt ctgttttaaa
     6841 attatttagt cacgcaaagc tgatgtggat tgtgctgttt tacgagtgag ttatatgcac
     6901 agaagtgttg ttcaccaact taaatcctcc ggcaaaacgt tgatgtgact attttcagtt
     6961 tcataaggga ccttttacag catcgacaga ttattattta gtttaacaac aaagcatcag
     7021 ttaatagagc tattcctccg agcctgcttt ggttcatttt tgaacaatga caccctataa
     7081 tttttatcat gctactttga atatttgata tgaaatagca cgcaagtatg gcttagtaat
     7141 aagtgtaatt tttgcacgcc gccagccaac cactaactac atttctagct ggcgaatgac
     7201 attaactaat aggttgtctg ctgtcatctt taaggtgcgc acgtttgtgt gcatgcaaaa
     7261 gcatggcttt ggacagcagg ggagggactt tgttttaaag atatcatgct agccggttag
     7321 tattttggca tatcacctac tacaccctta atgagtgcta atgatgacct tttgaatgat
     7381 aatattaact atgtagatta acgcaccaaa aatgtaaaac cgcgagccct gaacgtgata
     7441 gttttctgaa ataaaatcca tgtttacacc aaagatggtt gctataacta tagtgatgac
     7501 gatattaaca atgcacaata attaaataat gtgctttaac tactataaaa ttcattaatg
     7561 aaattgacct gggcgtcacg gtagcgcaat gtttagcaca atcgcctcac agtaagaagg
     7621 tcactggttc gagcctgggc tgggtcagtt ggcatttctg tctggagatt gcatgttctc
     7681 cccgtatttg catgctccag tttaccccac aagtccaaag acatgtggta taggtgaatt
     7741 gaaaaggcta aattgtccct agtgcatgtg tgtgaatgag tgtgcatgga tgtttaccat
     7801 tgatgggttg cagatgaaag gacataatct gcgtaaaaca tatgctggat aagttggtgg
     7861 ttcattccac tgtgtacagc ctttaaaggg atagttcacc taaaattgaa atatctgtca
     7921 tcgtttactc acccttcaca tataacaaac cagtttgagt ttcttttttc tgttgaacac
     7981 aaaaaagaag tgattttgat aaatggaaac ctgcaaccat tgactcctat cgtatttgtt
     8041 tttcatacta tgggtggtta cacatggttg caggtttctt taatttatca tgtataatat
     8101 atgtcttgtg gcatggtggc tcagtggtta gcactgttgc ctcacagcaa gaaggtcact
     8161 gggttgagtc ccagctgggc cagatagcat ttctgtgtgg tgtttgcatg tttaccctgt
     8221 gttggtgcgt gtttcccccg gtgctccggt ttcccccaca atttaaagac atgtgatata
     8281 gatgaattga ataaactaaa ttggccatat gagtgtgtga atgagagtgt atggctgttt
     8341 cgcagtactg ggttgcagct agaagggcat tcgctgcgta aaacacatgc tggaatagtt
     8401 gctggttcat tcctctgtca caacttataa aatagagact aagcttaagg aaaatgaatg
     8461 aatgaatata ctgtatgtat ttttaaatat atcttctttg ttcaacacaa aaaaatgagc
     8521 tccataaaga tttggaaaca cttgagggtg agtaaataaa cattttgtgg tgaaatttgc
     8581 ttttaagtct gacagttgtc ttgtgaagta tataaacagg atattatgca tgtgtttggt
     8641 agtttaattg taagattaca tttacactgc atgttcattt tgaccaaaaa ctaagcaaca
     8701 gcggttttga tattttcaag gtcaagttga aatggaggtg ggccatgttt cactattaga
     8761 gcagccagaa cgtcagtatt agtcattata taatctgtaa ttatatgctt gggtagcgtg
     8821 gtttggatgg tgtctgaggt tctgctgtga gtttgtcagt gggctttata tggtaacttg
     8881 cagctacagt atgttaacca caaatctcgg catcagtcaa cttggagggc atcttctccg
     8941 aaagtatgca gcctaaaatg catgttaagc gaattacaaa attacggaag atcaggtttt
     9001 gccatagagg tgatatacag tgatccctaa aacctgaatt tccatattca aagcatttag
     9061 caatgagctc ataagaaagt gacccaaact ccaacgtaga tggacagaca cacagaggga
     9121 ggaagtaaaa gagagagaaa gaaaaagaga aaagatctgt ctgctggctg aatgagcacc
     9181 tccttttggt tcggccacag tcccctcctc cctgcttcag atatagagtg tttttaggag
     9241 cagtagagtc aatcagagcc gggtgtacag gagagcgcac tgtcaggata tttcatcctt
     9301 ctgcacacac acacatacac caacacacac attttcttac gttctccctc tccaacggat
     9361 ccactagttc tagagcggcc gccaccgcgg tggccagtga cgtaggaagt ccatccattc
     9421 acagcgcttc tataaaggcg ccagctgagg cgcctactac tccaaccgcg actgcagcga
     9481 gcaactgaga agactggata gagccggcgg ttccgcgaac gagcagtgac cgcgctccca
     9541 cccagctctg ctctgcagct cccaccagtg tctacccctg gaccccttgc cgggctttcc
     9601 ccaaacttcc caccgcggtg gagctccagc ttttgttccc tttagtgagg gttaattcaa
     9661 gtttgtacaa aaaagcaggc tcaacatgcc acccaagcgc aaaacgctta acgctgcggc
     9721 tgaggctaac gctcatgccg acggacacgc cgacggaaac gccgacggac acgtggccaa
     9781 tacggccgcg tcctcgaata atgcgaggtt cgctgatctc actaacatcg atactccggg
     9841 tctgggaccc acaactacga ccctgctcgt ggaaccagca cgctcaaagc gtcaacgagt
     9901 gtcccgcgca tgcgaccagt gccgtgcagc ccgagagaaa tgcgacggaa tacagcctgc
     9961 gtgtttcccg tgcgtttccc agggaaggtc ctgcacttat caggcttcgc cgaaaaagag
    10021 gggagttcaa accggttata ttcgtacgct ggagctcgcc ctcgcctgga tgtttgaaaa
    10081 tgtcgcgcgt tccgaagatg ccttgcataa cctcctcgtc cgtgacgccg gacaaggatc
    10141 agctctgctc gttggtaaag attcgccggc tgccgagcga ctccatgccc gttgggctac
    10201 tagccgtgtc aataagagca ttacccgcct cctccgtcag ttggagctcc ctcctaccgc
    10261 cacggctacg gcctcgataa tgccgcacgt gatggagcag cctctcagta ccagcattaa
    10321 ccccgtcaac gaccgcttca acggtattcc caaccccact ccgtataact ccgatgcagc
    10381 tctcgatgct atcactcaga ccaacgatta tggaagcgta aatacacatg gtatcctctc
    10441 tacttacccg ccaccggcta cgcaccttaa tgaagcttcc gtcgctctcg ctcccggtgg
    10501 cgcccccccc cgaccgcctc ctccgtatgt tgacagcacg accaatcacc cgccgtacca
    10561 ctcgaatctg gttccaatgg cgaactttgg ttactcgacc gttgattacg atgccatggt
    10621 tgacgatttg gctagcattg aatacacgga cgctgtggat gtcgacccac agtttatgac
    10681 caatctggga ttcgttcctg gatgtaactt ctccgacatt aatacatacg aacagtgatg
    10741 acgtctatcg ataccgtcga ctaaagccaa cccagctttc ttgtacaaag tgggggatcc
    10801 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
    10861 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
    10921 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
    10981 ggaggttttt tnccaacttt mttrtacaaa gtgggggatc cagacatgat aagatacatt
    11041 gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt
    11101 tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac
    11161 aattgcattc attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttccaacttt
    11221 a
//
