LOCUS       sequence-485702-        8231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Plasmid for making PiggyBac stable cell line expressing Cristoli 
            virus (CRIV) glycoprotein Gc spike with a C-terminal His6 tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8231)
  AUTHORS   Merchant MK, de Paula Souza J, Abdelkarim S, Chakraborty S, Nasser 
            Neto TA, Gutierrez Manchay KI, de Melo Jorge DM, do Nascimento VA, 
            Sun Y, Caroe ER, Eyssen LEA, Rudd JS, Ribeiro Piauilino IC, 
            Damasceno Pinto S, Pereira da Silva MH, Rocha do Nascimento F, 
            Naveca FG, Proenca-Modena JL, da Silva LLP, Pinto de Figueiredo RM, 
            Owens RJ, Arruda E, Graham SC
  TITLE     Protein-based tools for the detection and characterisation of 
            Oropouche virus infection.
  JOURNAL   EMBO Mol Med. 2025 Aug 11. doi: 10.1038/s44321-025-00291-7.
  PUBMED    40790101
REFERENCE   2  (bases 1 to 8231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO Mol 
            Med. 2025 Aug 11. doi: 10.1038/s44321-025-00291-7."
COMMENT     Sequence Label: PB CRIV Gc Spike H6
FEATURES             Location/Qualifiers
     source          1..8231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(28..456)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(138..159)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     583..605
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     597..614
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     598..614
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     621..640
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        621..639
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   706..768
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1140..1158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1177..1195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1214..1232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1251..1269
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1288..1306
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1325..1343
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1362..1380
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    1399..1417
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     1439..1463
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      1562..1571
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2906..2923
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     misc_feature    2957..3545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3010..3030)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3428..3439)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3639..3656)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3645..3869
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    4153..4200
                     /label=FRT
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     rep_origin      4294..4428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4433..5035
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MGTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIE
                     RVTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRL
                     AAQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLET
                     SAPRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     4932..4952
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    5269..5390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5306..5325)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5858..5892
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6041..6059)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6080..6096)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6080..6096)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6093..6115)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6104..6120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6128..6134,6135..6152,6153..6158))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6173..6194
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6311..6328)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6482..7070)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6562..6581)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7241..8032,8033..8101))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7864..7883
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8102..8206)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat
       61 tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga
      121 tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca
      181 acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct
      241 aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc
      301 cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag
      361 cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca
      421 cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca
      481 actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg
      541 gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta
      601 aaacgacggc cagtgaattg taatacgact cactataggg cgaattggag ctcggtattc
      661 acgacagcag gctgaataat aaaaaaatta gaaactatta tttaacccta gaaagataat
      721 catattgtga cgtacgttaa agataatcat gcgtaaaatt gacgcatgtg ttttatcggt
      781 ctgtatatcg aggtttattt attaatttga atagatatta agttttatta tatttacact
      841 tacatactaa taataaattc aacaaacaat ttatttatgt ttatttattt attaaaaaaa
      901 aacaaaaact caaaatttct tctataaagt aacaaaactt ttaaacattc tctcttttac
      961 aaaaataaac ttattttgta ctttaaaaac agtcatgttg tattataaaa taagtaatta
     1021 gcttaaccta tacataatag aaacaaatta tacttattag tcagtcagaa acaactttgg
     1081 cacatatcaa tattatgctc tcgttaatta actcgaggga tccaagctgg ccgcacgtct
     1141 ccctatcagt gatagagaag tcgacacgtc tcgagctccc tatcagtgat agagaaggta
     1201 cgtctagaac gtctccctat cagtgataga gaagtcgaca cgtctcgagc tccctatcag
     1261 tgatagagaa ggtacgtcta gaacgtctcc ctatcagtga tagagaagtc gacacgtctc
     1321 gagctcccta tcagtgatag agaaggtacg tctagaacgt ctccctatca gtgatagaga
     1381 agtcgacacg tctcgagctc cctatcagtg atagagaagg taccccctat ataagcagag
     1441 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
     1501 gaagacaccg ggaccgatcc agcctccgcg gtggcggcca tcacaagttt gtacagctag
     1561 cgccaccatg gccaacctga tcatcatcag catggtgctg ggcgtcgcct acggcacaga
     1621 gtttgagaca tgcatcaaga aagtcgagat gacgcccgag tgcacaggcc cctttatgga
     1681 agtgggcatc tgcaaccacc agcagaagtc cctgagatac cacgagatcg ccaacatgct
     1741 gctcggcaag ggcaccatca gcaagctgga cgtggacatc atcgacgccc tgcctgataa
     1801 gattgccggc gacctgcaag tgatccagag cagcagcgat ttccatcatc agcagatgct
     1861 ggaatacgtg ttcctgctgc ggcactgcga ctactacagc agcttcgtgt ccgacaacag
     1921 ctacagccag atcctgtggc agacagccgc caagctgaac gagatcaacc tgtgcaagca
     1981 cgagagcagc aagaaaagct gcaagtgcct gaagtccaac gccaactgtg gcgagagcga
     2041 ggacatcaat actgccgctc aggcctacta caagaacaaa gagagcgaac tcggccagga
     2101 cctgaccacc atcatgtaca tcctgaagaa gatgatcccc ggcagcggct acagctacct
     2161 gctgaatgcc accaagcaca acaagctgga actgttcaag gcctacatga ccaacatcag
     2221 cagcaagtac cccaacaaca agaaactgaa ggacttcgtg gccatcacca acgccattct
     2281 ggccgatctg tctgccctga gccacagcag cgaggaatct atccccgagt acaagcagat
     2341 cgacttcagc agcctcgtgg gcgagacaac cgatatgctg gaagccaact tcaaccagac
     2401 catctccacc gacaagatcg tgtgcgagaa ccccaaagtg atgatctgca tcagccccag
     2461 agtgtccagc ggcgagctgt acgtgtgtca gaagctgggc aagtactata tcatcgacac
     2521 cggcaggttc aagctgtaca agctggtgga cgccgacaac ctgtactgtg ccgctgatgc
     2581 ccagtgcaga tacgagttca gaatcctgac cacaggcgag atgcaggcca ccttcaagga
     2641 caactgcaag gccagcatcc acaccaacac acccggctac cagaacgagg ccatcacaca
     2701 ctgtaaagtg ctggccaacg gcaagtgcaa atacgacaat cagaccctga tcgacgtgac
     2761 cctgtgcgcc aacaaccggt actaccctga ggccaagggc agaatctttg gcgtggaccc
     2821 tgaggacctg tgcttcgaca agacctgcac caagctgcag accccttaca acgaggaact
     2881 gatctctatg tgcgtgtggg agaagcatca ccaccatcat cactgactta agggtaccga
     2941 attcgatatc aagcttaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat
     3001 tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca
     3061 tgctattgct tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc
     3121 tctttatgag gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc
     3181 tgacgcaacc cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt
     3241 cgctttcccc ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg
     3301 gacaggggct cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc
     3361 ctttccatgg ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta
     3421 cgtcccttcg gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg
     3481 gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc
     3541 cccgcgcggc cgcatagata actgatccag tgtgctgggc tacgatgcat gggcccccta
     3601 ggtcgagtct agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     3661 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     3721 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     3781 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     3841 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctggggctc
     3901 tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     3961 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     4021 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     4081 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     4141 ttcacgtacc tagaagttcc tattccgaag ttcctattct ctagaaagta taggaacttc
     4201 cttggccaaa aagaccggta ccaggcaggc agaagtatgc aaagcatgca tctcaattag
     4261 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     4321 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     4381 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcta ccatgggtac
     4441 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtcccccggg ccgtacgcac
     4501 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgacc cggaccgcca
     4561 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     4621 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     4681 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     4741 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     4801 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     4861 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     4921 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     4981 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     5041 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatggct ccgaccgaag
     5101 ccacccgggg cggccccgcc gaccccgcac ccgcccccga ggcccaccga ctctagataa
     5161 ctgatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca
     5221 cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt
     5281 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     5341 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta acgctcgagt
     5401 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gtataccgtc
     5461 gacctctagc tgcaggaatt cgatatcaag ctctagccaa caagctcgtc atcgctttgc
     5521 agaagagcag agaggatatg ctcatcgtct aaagaactac ccattttatt atatattagt
     5581 cacgatatct ataacaagaa aatatatata taataagtta tcacgtaagt agaacatgaa
     5641 ataacaatat aattatcgta tgagttaaat cttaaaagtc acgtaaaaga taatcatgcg
     5701 tcattttgac tcacgcggtc gttatagttc aaaatcagtg acacttaccg cattgacaag
     5761 cacgcctcac gggagctcca agcggcgact gagatgtcct aaatgcacag cgacggattc
     5821 gcgctattta gaaagagaga gcaatatttc aagaatgcat gcgtcaattt tacgcagact
     5881 atctttctag ggttaaaaaa gatttgcgct ttactcgacc taaactttaa acacgtcata
     5941 gaatcttcgt ttgacaaaaa ccacattgtg gccaagctgt gtgacgcgac gcgcgctaaa
     6001 gaatggcaaa ccaagtcgcg cgaggtacca gcttttgttc cctttagtga gggttaattt
     6061 cgagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa
     6121 ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga
     6181 gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
     6241 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     6301 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     6361 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     6421 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     6481 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     6541 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     6601 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     6661 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     6721 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     6781 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     6841 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     6901 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     6961 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     7021 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     7081 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     7141 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     7201 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     7261 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     7321 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     7381 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     7441 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     7501 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag
     7561 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     7621 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     7681 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     7741 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     7801 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac
     7861 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
     7921 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
     7981 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
     8041 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
     8101 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
     8161 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     8221 aagtgccacc t
//
