LOCUS       sequence-485992-       12423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  To express tagBFP-2A-FLAG-coBICP0 (co = codon-optimized).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12423)
  AUTHORS   Lahaye X, Tran Van P, Chakraborty C, Shmakova A, Cao NTB, Ferran H, 
            Ait-Mohamed O, Maurin M, Waterfall JJ, Kaufer BB, Fischer P, Hennig 
            T, Dolken L, Lomonte P, Fachinetti D, Manel N
  TITLE     Centromeric DNA amplification triggered by viral proteins activates 
            nuclear cGAS.
  JOURNAL   Cell. 2025 May 29:S0092-8674(25)00556-2. doi: 
            10.1016/j.cell.2025.05.008.
  PUBMED    40460826
REFERENCE   2  (bases 1 to 12423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            May 29:S0092-8674(25)00556-2. doi: 10.1016/j.cell.2025.05.008."
COMMENT     Sequence Label: pTRIP-SFFV-tagBFP-2A-FLAG-coBICP0
FEATURES             Location/Qualifiers
     source          1..12423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     intron          43..108
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             238..258
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    683..817
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(720..739)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     774..793
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             985..1618
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    1665..1790
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2283..2516
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2701..2745
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3038..3155
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3295..3702
                     /label=SFFV promoter
                     /note="spleen focus-forming virus long terminal repeat 
                     (LTR) promoter"
     CDS             3721..4419
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             4429..4485
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     regulatory      4498..4507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4507..4530
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     LTR             6827..7007
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8535..8554)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(8768..8787)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8887..8909
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8947..8965)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9033..9137
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9138..9206,9207..9998)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9356..9375)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10169..10757
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10658..10677
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10911..10928
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(10998..11018)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        11003..11332
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      11183..11318
                     /label=SV40 ori
                     /note="SV40 origin of replication"
ORIGIN
        1 acataattgg acaaactacc tacagagatt taaagctcta aggtaaatat aaaattttta
       61 agtgtataat gtgttaaact actgattcta attgtttgtg tattttagat tccaacctat
      121 ggaactgatg aatgggagca gtggtggaat gcctttaatg aggaaaacct gttttgctca
      181 gaagaaatgc catctagtga tgatgaggct actgctgact ctcaacattc tactcctcca
      241 aaaaagaaga gaaaggtaga agaccccaag gactttcctt cagaattgct aagttttttg
      301 agtcatgctg tgtttagtaa tagaactctt gcttgctttg ctatttacac cacaaaggaa
      361 aaagctgcac tgctatacaa gaaaattatg gaaaaatatt ctgtaacctt tataagtagg
      421 cataacagtt ataatcataa catactgttt tttcttactc cacacaggca tagagtgtct
      481 gctattaata actatgctca aaaattgtgt acctttagct ttttaatttg taaaggggtt
      541 aataaggaat atttgatgta tagtgccttg actagagatc ataatcagcc ataccacatt
      601 tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa
      661 aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag
      721 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
      781 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
      841 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
      901 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
      961 aatatgtact acaaacttag tagttggaag ggctaattca ctcccaaaga agacaagata
     1021 tccttgatct gtggatctac cacacacaag gctacttccc tgattagcag aactacacac
     1081 cagggccagg ggtcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg
     1141 agccagataa ggtagaagag gccaataaag gagagaacac cagcttgtta caccctgtga
     1201 gcctgcatgg gatggatgac ccggagagag aagtgttaga gtggaggttt gacagccgcc
     1261 tagcatttca tcacgtggcc cgagagctgc atccggagta cttcaagaac tgctgatatc
     1321 gagcttgcta caagggactt tccgctgggg actttccagg gaggcgtggc ctgggcggga
     1381 ctggggagtg gcgagccctc agatcctgca tataagcagc tgctttttgc ctgtactggg
     1441 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1501 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1561 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1621 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     1681 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     1741 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     1801 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     1861 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     1921 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     1981 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     2041 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     2101 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     2161 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2221 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2281 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2341 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2401 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2461 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2521 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2581 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2641 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2701 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2761 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2821 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2881 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     2941 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg
     3001 tatcgccgaa ttcacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     3061 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     3121 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     3181 atccactttg gctgatacgc gtctgcagcc ccgataaaat aaaagatttt atttagtctc
     3241 cagaaaaagg ggggaatgaa agaccccacc tgtaggtttg gcaagctagc tgcagtaacg
     3301 ccattttgca aggcatggaa aaataccaaa ccaagaatag agaagttcag atcaagggcg
     3361 ggtacatgaa aatagctaac gttgggccaa acaggatatc tgcggtgagc agtttcggcc
     3421 ccggcccggg gccaagaaca gatggtcacc gcagtttcgg ccccggcccg aggccaagaa
     3481 cagatggtcc ccagatatgg cccaaccctc agcagtttct taagacccat cagatgtttc
     3541 caggctcccc caaggacctg aaatgaccct gcgccttatt tgaattaacc aatcagcctg
     3601 cttctcgctt ctgttcgcgc gcttctgctt cccgagctct ataaaagagc tcacaacccc
     3661 tcactcggcg cgccagtcct ccgacagact gagtcgcccg ggccgcgggc tagcgccacc
     3721 atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg caccgtggac
     3781 aaccatcact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg cacccagacc
     3841 atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat cctggctact
     3901 agcttcctct acggcagcaa gaccttcatc aaccacaccc agggcatccc cgacttcttc
     3961 aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga agacgggggc
     4021 gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta caacgtcaag
     4081 atcagagggg tgaacttcac atccaacggc cctgtgatgc agaagaaaac actcggctgg
     4141 gaggccttca ccgagacgct gtaccccgct gacggcggcc tggaaggcag aaacgacatg
     4201 gccctgaagc tcgtgggcgg gagccatctg atcgcaaacg ccaagaccac atatagatcc
     4261 aagaaacccg ctaagaacct caagatgcct ggcgtctact atgtggacta cagactggaa
     4321 agaatcaagg aggccaacaa cgagacctac gtcgagcagc acgaggtggc agtggccaga
     4381 tactgcgacc tccctagcaa actggggcac aagcttaatg gaagcggagc tactaacttc
     4441 agcctgctga agcaggctgg agacgtggag gagaaccctg gacctggatc cactagtgcc
     4501 accatggatt ataaggatga cgatgacaag gctccaccag cagccgctcc cgaattgggg
     4561 tcctgttgta tttgtttgga tgcaattaca ggtgccgctc gggcccttcc ttgtctccat
     4621 gcattttgtt tggcgtgtat tcggagatgg ctcgaaggta gacctacatg tcctctctgt
     4681 aaagctccag tccaatccct gatacattcc gtggcttctg acgaatgttt tgaagaaatt
     4741 ccagtcggcg gcggccccgg cgcagatggt gccttagaac ctgatgctgc tgtgatttgg
     4801 ggcgaagatt atgatgctgg accaattgat cttaccgccg cggatggtga agcttccggt
     4861 gctggcggcg aggcaggcgc ggcagatggc tctgaagcag gaggaggagc aggtggcgcc
     4921 gaagaagccg gcgaagctag gggcgcagga gccggacgag ctgcaggggc cgcaggcggc
     4981 cgtgccggta gaggggccga tgccgcacaa gaattcatcg atagagtggc ccgaggtcct
     5041 cgactcccac tgttacccaa tacaccagaa cacggacccg gagcacccta tctccgcaga
     5101 gtcgtcgaat gggtggaagg tgcccttgtt ggaacattcg ctgttactgc tagagaactg
     5161 gccgctatga ctgattatgt catggcaatg cttgctgaat gtgggtttga tgatgatgga
     5221 cttgcggatg caatggaacc attgatcggg gaagatgatg caccagcatt cgtccgaagt
     5281 ttattgtttg tcgctgctcg atgtgttacc gttgggccta gccatctgat tccgcaacaa
     5341 agcgccccgc ccggcggaag gggcgttgtt ttccttgata caagcgatag cgattcagaa
     5401 ggaagtgaag atgattcctg gtcagaatct gaggaaagct ccagtggact gagtacaagc
     5461 gatctcactg ctatcgatga tactgagact gaaccagaaa ccgatgctga agtcgaaagc
     5521 cgcagaactc gcggagccag cggagctgcg agagccagaa gaccagccga gaggcaatac
     5581 gtgagtacga gaggcagaca gaccccggcc gttcaacctg cccccaggag tttagcaagg
     5641 cggccctgtg ggcgggctgc cgctgtgagc gccccaccat cttccaggtc taggggcgga
     5701 cggagagatc caaggttgcc agccgcacca agagctgctc cagcagcaca ggcccgggcg
     5761 tgttcacctg aacctcgcga ggaaggcaga ggcgcaggac tcggggtggc agccggtgaa
     5821 actgcagggt ggggcgccgg atcagaagaa gggagaggcg aaaggagagc acgattgctg
     5881 ggagaagccg gaccaccacg ggttcaagct cggcggcgac gtcggacgga attggatcgt
     5941 gctcctacac ctgcacccgc acctgctccg gcgccggctc cgatttctac cgttattgat
     6001 ttgaccgcca atgcaccggc acgacctgca gatcccgcgc cagccgccgc tcctggaccc
     6061 gcttcagctg gcgcccagat cggaacgccc gcagctgccg ctgccgttac cgctgctgct
     6121 gccgccccat cagttgccag gagttccgcc ccatcacctg cggtgacagc agccgcaacg
     6181 tctactgctg ctgccataag tactcgcgca cccacacctt ccccggctgg aagggcacca
     6241 gctgcagatc ctagacgcgc tggagcaccc gctcttgcgg gcgctgctcg cgcagaagtg
     6301 ggacgaaacg gaaatccggg gagagaacga cgccctgcat ccgctatggc cagaggagat
     6361 ttagatccgg ggcctgaaag ttccgctcag aaacggcgcc gaacagagat ggaagtcgcg
     6421 gcgtgggttc gcgagtcctt acttggaact cctcgacgtt catccgcagc attggctccc
     6481 caacctggtg gaaggcaagg gcccagttta gctggactcc tgggtcgttg ttctggcggc
     6541 tccgcgtggc ggcagtgact cgagacctag aaaaacatgg agcaatcaca agtagcaata
     6601 cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt
     6661 ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta
     6721 gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaaa
     6781 atcgtcgaga gatgctgcat ataagcagct gctttttgct tgtactgggt ctctctggtt
     6841 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     6901 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     6961 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     7021 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggcc
     7081 ttgacattat aatagattta gcaggaattg aactaggagt ggagcacaca ggcaaagctg
     7141 cagaagtact tggaagaagc caccagagat actcacgatt ctgcacatac ctggctaatc
     7201 ccagatccta aggattacat taagtttact aacatttata taatgattta tagtttaaag
     7261 tataaactta tctaatttac tattctgaca gatattaatt aatcctcaaa tatcataaga
     7321 gatgattact attatcccca tttaacacaa gaggaaactg agagggaaag atgttgaagt
     7381 aattttccca caattacagc atccgttagt tacgactcta tgatcttctg acacaaattc
     7441 catttactcc tcaccctatg actcagtcga atatatcaaa gttatggaca ttatgctaag
     7501 taacaaatta cccttttata tagtaaatac tgagtagatt gagagaagaa attgtttgca
     7561 aacctgaata gcttcaagaa gaagagaagt gaggataaga ataacagttg tcatttaaca
     7621 agttttaaca agtaacttgg ttagaaaggg attcaaatgc ataaagcaag ggataaattt
     7681 ttctggcaac aagactatac aatataacct taaatatgac ttcaaataat tgttggaact
     7741 tgataaaact aattaaatat tattgaagat tatcaatatt ataaatgtaa tttactttta
     7801 aaaagggaac atagaaatgt gtatcattag agtagaaaac aatccttatt atcacaattt
     7861 gtcaaaacaa gtttgttatt aacacaagta gaatactgca ttcaattaag ttgactgcag
     7921 attttgtgtt ttgttaaaat tagaaagaga taacaacaat ttgaattatt gaaagtaaca
     7981 tgtaaatagt tctacatacg ttcttttgac atcttgttca atcattgatc gaagttcttt
     8041 atcttggaag aatttgttcc aaagactctg aaataaggaa aacaatctat tatatagtct
     8101 cacacctttg ttttactttt agtgatttca atttaataat gtaaatggtt aaaatttatt
     8161 cttctctgag atcatttcac attgcagata gaaaacctga gactggggta atttttatta
     8221 aaatctaatt taatctcaga aacacatctt tattctaaca tcaatttttc cagtttgata
     8281 ttatcatata aagtcagcct tcctcatctg caggttccac aacaaaaatc caaccaactg
     8341 tggatcaaaa atattgggaa aaaattaaaa atagcaatac aacaataaaa aaatacaaat
     8401 cagaaaaaca gcacagtata acaactttat ttagcattta caatctatta ggtattataa
     8461 gtaatctagc cagatcctct acgccggacg catcgtggcc ggcatcaccg gcgccacagg
     8521 tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg ctcgccactt
     8581 cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg ccgggggact
     8641 gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca acggcctcaa
     8701 cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc gaatggtgca
     8761 ctctcagtac aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac
     8821 ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga
     8881 ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac
     8941 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt
     9001 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     9061 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     9121 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     9181 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     9241 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     9301 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     9361 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     9421 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     9481 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     9541 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     9601 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     9661 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     9721 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     9781 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     9841 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     9901 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     9961 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
    10021 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
    10081 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
    10141 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
    10201 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
    10261 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
    10321 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
    10381 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
    10441 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
    10501 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
    10561 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
    10621 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
    10681 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
    10741 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
    10801 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
    10861 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
    10921 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
    10981 ttcattaatg cagctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
    11041 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
    11101 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
    11161 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
    11221 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
    11281 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttgg
    11341 acacaagaca ggcttgcgag atatgtttga gaataccact ttatcccgcg tcagggagag
    11401 gcagtgcgta aaaagacgcg gactcatgtg aaatactggt ttttagtgcg ccagatctct
    11461 ataatctcgc gcaacctatt ttcccctcga acacttttta agccgtagat aaacaggctg
    11521 ggacacttca catgagcgaa aaatacatcg tcacctggga catgttgcag atccatgcac
    11581 gtaaactcgc aagccgactg atgccttctg aacaatggaa aggcattatt gccgtaagcc
    11641 gtggcggtct gtaccgggtg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac
    11701 cgtttgtatt tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc
    11761 agaaggcgat ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc
    11821 ggttgcgatt cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc
    11881 tggtcgtccg ctggttgatg actatgttgt tgatatcccg caagatacct ggattgaaca
    11941 gccgtgggat atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg
    12001 cctggcactg ccgggcgttg ttctttttaa cttcaggcgg gttacaatag tttccagtaa
    12061 gtattctgga ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc
    12121 gctttaaaca tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc
    12181 ctgtgcagtc ggcccttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc
    12241 tgatgtggat ctggcgcggc attgacccac gcgaaatcct cgacgtccag gcacgtattg
    12301 tgatgagcga tgccgaacgt accgacgatg atttatacga tacggtgatt ggctaccgtg
    12361 gcggcaactg gatttatgag tgggccccgg atctttgtga aggaacctta cttctgtggt
    12421 gtg
//
