LOCUS       sequence-486047-       18890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Acceptor vector II, accepting Donor II first, with attBCC-FRT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 18890)
  AUTHORS   Zhao C, Stepanova AN, Alonso JM
  TITLE     DASH: a versatile and high-capacity gene stacking system for plant 
            synthetic biology.
  JOURNAL   Plant Biotechnol J. 2025 Jun 10. doi: 10.1111/pbi.70179.
  PUBMED    40493021
REFERENCE   2  (bases 1 to 18890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Biotechnol J. 2025 Jun 10. doi: 10.1111/pbi.70179."
COMMENT     Sequence Label: pDASH-AIIK
FEATURES             Location/Qualifiers
     source          1..18890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    451..475
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    complement(657..690)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     misc_feature    780..804
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             complement(9752..10570)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVG
                     IADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     10478..10497
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     10562..10581
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    16995..17016
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(17031..17036,17037..17054,17055..17061)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17069..17085
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     17074..17096
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     17093..17109
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     17093..17109
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     18723..18741
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 ttgcttcctg tcggccctca ttcgtgcgct ctaggatcga cgatcgtcaa cgttcacttc
       61 taaagaaata gcgccactca gcttcctcag cggctttatc cagcgatttc ctattatgtc
      121 ggcatagttc tcaagatcga cagcctgtca cggttaagcg agaaatgaat aagaaggctg
      181 ataattcgga tctctgcgag ggagatgata tttgatcaca ggcagcaacg ctctgtcatc
      241 gttacaatca acatgctacc ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt
      301 tgccgttctt ccgaatagca tcggtaacat gagcaaagtc tgccgcctta caacggctct
      361 cccgctgacg ccgtcccgga ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag
      421 ctgccggtcg gggagctgtt ggctggctgg tggcaggata tattgtggtg taaacaaatt
      481 gacgcttaga caacttaata acacattgcg gacgttttta atgtactgaa ttctagtagt
      541 gccccaactg gggtaacctt tgggctcccc gggcgcgtac tccggtctca ggaggtcatg
      601 agaccccgcg gtgcgggtgc cagggcgtgc ccccgggctc cccgggcgcg tactccgaag
      661 ttcctatact ttctagagaa taggaacttc gaattcgtag ggataacagg gtaattccct
      721 taattctccg ctcatgatca gattgtcgtt tcccgccttc agtttaaact atcagtgttt
      781 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
      841 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
      901 ggttcccctc gggagtgctt ggcattccgt gcgataatga cttctgttca accacccaaa
      961 cgtcggaaag cctgacgacg gagcagcatt ccaaaaagat cccttggctc gtctgggtcg
     1021 gctagaaggt cgagtgggct gctgtggctt gatccctcaa cgcggtcgcg gacgtagcgc
     1081 agcgccgaaa aatcctcgat cgcaaatccg acgctgtcga aaagcgtgat ctgcttgtcg
     1141 ctctttcggc cgacgtcctg gccagtcatc acgcgccaaa gttccgtcac aggatgatct
     1201 ggcgcgagtt gctggatctc gccttcaatc cgggcctgtg gcgggaactc cacgaaaata
     1261 tccgaacgca gcaagatcgt cgatcctccg gcgttcagcc tgtgccacag ccgacaggat
     1321 ggtgaccgaa tcctacaagg tagaatccgc ctgagtcgca agggtgactt cgcctatatt
     1381 ggacgacggc gcgcagaggg cgacctcttt ttgggttacg attgtaggat tatcactaaa
     1441 acaatacatg aacatattca aatggcaatc tctctaaggc attggaaata aatacaaata
     1501 acagttgggt ggagtttttc gacctgaggg cgttaacctt ctgttaacct aaaagctctt
     1561 gcccaaacag cagaatcggc gctaattgcc agcggcggaa cttttccagt ttcgcgaaaa
     1621 gtatcgccac tggcaaggaa tgggtttgag atggcgaagt ctgtcctaaa agcagcgcct
     1681 gtagttgtag ggttgacggc cttgatggag cgtcatgccg atgccctctc gagccaactt
     1741 caagcacatc atcttaaggt tttcccgccg cattccgaga agggcattcg aacattcggg
     1801 ccatcggagg cgtccaagct gctcggcgtt ggcgagtcat atttacggca gaccgcgtct
     1861 gagatgccag agttgaatgt tagcatgagc ccgggtggca ggcgaatgtt ctcaattgaa
     1921 gatatccatg tgattcggaa gtatatggat caggtcggcc gcgggaaccg gcgctacctg
     1981 ccacatcgtc gaggcggcga gcagcttcag gttatctctg tgatgaattt caaaggtggg
     2041 tcgggtaaga ccaccaccgc cgcgcatctg gcgcagtacc tcgctatgcg cggatatcga
     2101 gtcttggcca ttgatctcga tcctcaagcg agcctttctg cactctttgg gagccaaccg
     2161 gagacggacg ttggcccgaa cgaaacgctc tacggcgcta taaggtatga tgatgagcag
     2221 gtggcaatcg aacgagtcgt ccgagggact tacattcccg acctccacct gattcctggt
     2281 aaccttgagc tgatggagtt tgaacacgat acgccacgcg cgctgatgaa ccgcaaagag
     2341 ggcgacacgc tcttttatgg tcgcatcagc caagtaattg aagatatcgc ggataactat
     2401 gacgtcgtgg tcatcgactg ccctccccag cttgggtatc tcacgctatc cgcattgact
     2461 gcggcgacgt ccattcttgt cacggtccat ccgcagatgc tggatgtgat gtcgatgaac
     2521 cagtttctgg caatgacatc gaaccttttg cgtgaaatcg agaatgctgg cgccaagttc
     2581 aagtttaatt ggatgcgcta tctgataacc cgtttcgaac cgagcgacgg accacagaac
     2641 caaatggtag gttatctgcg gtcgattttt ggcgaaaatg tcctcaattt tccgatgctt
     2701 aaaaccaccg cggtttcgga cgctggcctg acaaaccaga ctctattcga agtggagcgt
     2761 ggcctgttca cgcgctcgac ctatgatcga gccttggagg cgatgaacgc cgtcaacgac
     2821 gagatcgaaa cactgatcaa aaaagcatgg ggtaggccca catgagccgg aagcacatcc
     2881 ttggcgtctc aactgacgcc cctgagacgt cgcccgccga caataggacg gcaaagaacc
     2941 gctccatgcc gctcctcggc gtaacaagga aggagcgcga tccggcaacg aagctcacag
     3001 cgaacattgg taacgcactg cgagagcaaa acgatcgtct tagccgtgcc gaagagatcg
     3061 agcggcgtct cgctgaaggt caggcagtga tagagttgga tgcctcgtca atagaaccgt
     3121 ctttcgtgca ggatcgtatg cgaggggaca ttgacgggct ccttacttcg atccgggaac
     3181 aaggacagca agtcccaatc cttgtgcgac cgcatccgag ccagccgggc cgatatcagg
     3241 ttgccttcgg ccaccgccgg ctacgcgccg tttcagaact cggacttccg gtcagagcgg
     3301 tcgttcgcga actgacggac gagcaagtgg tcgtagcaca gggtcaggaa aacaatgagc
     3361 gcgaagatct taccttcatc gaaaaggcgc gcttcgcaca tcgcctgaac aggcagtttt
     3421 ctcgagagat tgtcatcgcc gcgatgtcga tcgacaagag caatttgtcc aagatgcttc
     3481 tgctcgtcga cgccctcccc tctgaactga ccgatgctat tggtgccgct cctggtgttg
     3541 gacggccgag ttggcaacaa cttgccgagc tgattgagaa agtttcttca ccggccgacg
     3601 tggctaaata tgctatgtcg gaggaagttc aagcgctgcc atcggcagaa cgattcaagg
     3661 cggtgatcgc tagtctgaag cccagtcggg ttgcgcgtgg acttcccgag gtcatggcca
     3721 ccccagacgg caccagaatt gcacaggtga cgcagagcaa ggccaaactg gaaatcacga
     3781 ttgacaggaa ggcgacgccc gattttgcga ccttcgtgct cgatcatgtg ccagcgctgt
     3841 atcaagcgta ccacgctgag aaccaacgga aacggggaga gtaaaccgca aaagaaaaga
     3901 gccccctcaa cgtcgccgtc gcggaagccc ttctgtctct ctagcgcgaa cagaatcgca
     3961 tttcctcgaa tcctcgtcaa gagtttttag cgccgttttg gtgagctgat ttcctttgcc
     4021 tgctgaaagg tgaaagatga tgcagacagg aagtgtaacg acgccattcg ggcggcggcc
     4081 aatgacgctt gcgcttgtgc ggcgccagac ggcgctggcc gatatcaaac aaggcaagac
     4141 agcggacaag tggaaggtct ttagagacgc gtccgcggcc atggaactac ttggaatcca
     4201 gtccaacagt cttgccgtcc ttgatgcgct attgagcttt cacccggaaa cggagttgcg
     4261 tcaggaggca cagctgatcg tcttcccgtc gaatgctcag cttgcccttc gggcgcatgg
     4321 gatggctggc gcgactttgc gtaggcacat cgccatgctc gtggagtcag gcttgatcgt
     4381 ccggaaggat agcgccaacg gaaagcgtta cgctcgtaag gatggcgctg gtcagatcga
     4441 gcgcgcgttt ggcttcgatt tgtctccgct tctcgcgcgg tccgaagagc tagcgatgat
     4501 ggcacagcag gtgatggccg atcgagcagc attcaggatg gccaaagaaa gtctgacgat
     4561 ttgccgacgg gacgttcgga agctaattac ggcagctatg gaagagggag cggagggcga
     4621 ctggcaagct gtcgaggaag tctatgtgga acttgtgggt agaattccac gcgccccgac
     4681 gcttgctgat gtagagtcaa ttctcgaaga gatgtggatg ctccaggaag agataatcaa
     4741 ccggttggaa attagagaca attcagaaaa taatagcacc aatgctgccc agagcgagca
     4801 gcacatacag aattcaaaac ccgaatccgt taatgaactt gaacctcgct ctgaaaagga
     4861 gcagggcgct aagccgagtg aaatagaccg ggcagggagc gagccgataa aagcgttccc
     4921 cctcgggatg atcctgaaag catgcccgac cattggcaat tatgggccga gcggtgcggt
     4981 tgctagctgg cgtgacctca tgtcggctgc ggtggtggtt cggtctatgc tgggggtcag
     5041 cccgtcggct taccaagacg cgtgtgaggc aatgggaccg gagaatgcgg cagcagcgat
     5101 ggcgtgcatt ttggagcgag cgaacttcat caattcgccc gggggctatc tccgagatct
     5161 gacacggcgg agcgagctcg ggaagttttc acttggcccg atgataatgg cgctcttgaa
     5221 ggctagcggg caggggacgt tgcggtttgg ctagaattag cgagtatgga gcaggatggt
     5281 ctgtggtcag ctgaccacag acctaatagg ttgaaaacat gagcgttttt tggatgatcg
     5341 acagaccatc cgattcccgg agtaccaagc gtgctctgat gggagcgata acattactca
     5401 acaagcacga aggccccatg ccgatcgttg atcgtgaagg agagcctgct ctacatgcgg
     5461 cggtattttg ccggccgagg catgtagtcg cggagcactg cctatttact gccctaggca
     5521 caaacgttga ctcttggatc gagctggcag acaaagcaat aacccacaca gaggacgatt
     5581 aatggctgac gaagagatcc agaatccgcc ggacggtact gctgctgccg aagttgagcc
     5641 ggctgctcct agaggtagaa gagcaaagaa agcaccagcc gaaacagccc gcacgggatc
     5701 gttcaaatcc gtgaagccga aaacccgcgg cctcagcaac cgagaaaaac tggagaagat
     5761 cggtcaaatc gaagctcagg tcgctggcgg cgcaaccttg aaggacgccg ttaagatcgt
     5821 gggtatttcc gttcagacct attatcaatg gaagagagct gcggttcaac ctgtctcaca
     5881 gaatccggcc gtgtctgttt cagttgacga tgaactcggc gagttcatcc aactcgagga
     5941 ggaaaatcgg cggctcagaa agctacagct tctctctgaa aaattgcgcg cagaaaacgc
     6001 ggacttacgc aaaagacttg gaatcaacta gcggcctttg ggggtccctc gctaaatcaa
     6061 cttgacgcgc atctctcaat gaatgaatgc cttggaaaat ttccgcgcat tctgatcgtt
     6121 gcgctatcaa gcaatcccaa tcttctggca tatgaaaaaa ccacgaccca aattcgtcgt
     6181 cgaatataag acgaacaggc gacaagccaa ggcccgacca acgtcaatct ggggcaatct
     6241 gggtttgcag gcggtgggcc gtgcggtcgc ggccgacgga gtcgtgccga agagtagtct
     6301 gccgcaagtc cgttccgtcc tggagaaggt tgtcgctgca gatccagacg tggctgccat
     6361 ctttgctcag gttgagatta agggccttcc ggctcccccc aatacatcat caccgaacga
     6421 gggtcacatc gagccggtag ttattgaggg caaggtgttt gccgcgcctt tcgtgcagga
     6481 ggagcgcacc ttggccccgc cgcaggctcc gagatctcga gcgaaaccgc ggacaaaaag
     6541 gcggtccccg gacaccacag aacgtcctgc agtccaaggt taccgtcgtg gtccctcggt
     6601 gctgccaggt tttgaggaag agcttgccgc tatcgaggcg gaaaaccgtc gtctgaagcg
     6661 cctcttgatc gtgaagctcc gtgaggagaa cgacaggctt aagttcgcgc ttcgacgatt
     6721 tgggggcgcc tgagcacgat gctggcccac ttgcttcagc gggcaaacaa catggagtgg
     6781 agatctaatg atcgcggcgc ttgaacatcg ctaggggtct tcggcgccgc tgaaccgtgc
     6841 tccattttgc actcggagac acagcctccc aaaaacctgg cgccgctcgt tggtgaactc
     6901 ctcaacatcg agcggatcaa gggaaactgg aatgacatcc ttcgacccgt cacggcgatc
     6961 cgctctggcc aggtccaacc tacggccatg cccgcaaagc caacggcatt tccacgtcaa
     7021 gcacattgac ccactcggct gacggtacat cagctggcga gctcatccgg acccagacga
     7081 actgcgttga tctcaggccg ttgcggcaga aaacctccat cctcgcagcc gatccaacac
     7141 tttcctgtgg taatgcctat ttcagatgaa acagcctttt ttaaacttgg cgcgtcattc
     7201 tgatattatg gccacacctc tacgatctag ggagtgcgag aagtggaaag aaatgaagaa
     7261 cccgatttgc acacacatac ctcgatgcgg aataaaaatg catcatcgct tggtgaggcc
     7321 ctggatgacg atgcgatcgc caacatcgcg catcacattg ttacagacga cccggtggaa
     7381 acgtcaaaaa acttcacaaa acttaagctg attaacaaat cagtgaaaca gggtttcgaa
     7441 cgcagtgcgt acggaaaatt ccatcgacgc attagccggc ttggtattgc tagtaaggcg
     7501 ctatatgaca acgctgtgcc gaaggaagga ttcgtagaac atcctgaata tgctccagct
     7561 ctgcaaaggg attatgcgtt ggcaggagag cgaattgagg cggttggtgg aactttcagg
     7621 ttgcaaacac ctcagcggaa atcggctctt gtagatcata tcttgagtat gcccgaaggt
     7681 ttcgatcaag ctcatgccct tgagtctata gcccctcatg ttggcgatct aagcgcggaa
     7741 gatcgccatc gtgttgtgaa caaaatgctc gagcactttg ccaccccgtt tcaagaaggg
     7801 gggctgccaa ccgacgaaaa tttttgtgga acgtacgctt tgtccaaggc gtttcctcat
     7861 atggaagaca gcctaaaagc aaagatgctg gatgtggtgt tagagcatcc tcacttagcc
     7921 aacctccatt ggacgcagag agaactagtt gaagggtggc atcaagaccg tgaagctgag
     7981 aagtcagcct ggcgttccaa tcaaaagcca attgtcgagg ggcgacttgc agacctcgaa
     8041 cgatcgatcc acagtgatat cgaggtgaca aacaggagtg attttgcgag gatgcgttca
     8101 gccaggccct tgcaacaagc tataagcgat acgttcgatc ttgcgcgtga ggagctcaaa
     8161 agccgccctc gtgaagggcg gggtcgataa ttacatctat tggtctttct gccgaacctc
     8221 tatgaaatcc gcacgtcaaa tgtggctttt acttcccgat ttcaattgaa gcgctaagct
     8281 gaaaatcccg tccccatttc tgacctaacg gtttcatcac cggggcaggg agcggcgctt
     8341 aaaacggtga cggccaccac caaaggacat gttcgctcac gcggtgcctc cgtgcttctt
     8401 tgcaagagcc tgcttcaggc tgatgatctc cttcagtgcg ttggtgtgaa tccgctccag
     8461 gcgtttcagt tcgctctcca aataggcgtt gcgcttatcc agcctcgtcc gatcttcttc
     8521 caagctctcc attttcacta gcgcgttatg gtaattcaac cgtaactcct caaaaaaagc
     8581 acgttcggcg acgaaatggg tttcggaatc gagttcaggt atgtcgccga catgcacgac
     8641 gcgaaccgcc ttgccggcag cggcgagccg ggcgcggtaa gcgcgttgaa tttccgcttg
     8701 ggtcagcgcg tttggcttgc ggggccgcga ggaaatttgg gagtaaccat aacggttatt
     8761 tatcataacg cgttatcact ggatagcatc ggcgcaggcg gatggcgcgc ttcgccgggt
     8821 cgaaatcaat atttccagat cgagatggat tgaagacctg aagggccctc agagcccaga
     8881 cgccttggtt aggttggtgc ggaaccgatc ccgccgccgc tggtatttgc gcgtcatctc
     8941 ggccgaggcg tggcctagtt gtttctgaac gtaacgctcc tcaatgtcgg ccgaggaggc
     9001 aagaccggcg cgcagcgagt gccctgagaa cagcagcgcc cgttcccctt ctggaagatc
     9061 ggaacggacg ccggcggcga gtgcggtctg cttgaccagg cgggcgacat gcttgtcgga
     9121 gagccgctcg acgtccacgg tcttgttgtc cttgaagatg cgccggaaca ggggaccgcg
     9181 cgcgatcctg ccgaagcgga tcgtagtgac caccatttgc cccatatcac cgtcggtact
     9241 gatcccgtcg tcaataaacc gaaccgctac accctgagca tcaaactctt ttatcagttg
     9301 gatcatgtcg gcggtgtcgc ggccaagacg gtcgagcttc ttcaccagaa tgacatcacc
     9361 ttcctccacc ttcatcctca gcaaatccag cccttcccga tctgttgaac tgccggatgc
     9421 cttgtcggta aagatgcggt tagcttttac ccctgcatct ttgagcgctg aggtctgcct
     9481 cgtgaagaag gtgttgctga ctcataccag gcctgaatcg ccccatcatc cagccagaaa
     9541 gtgagggagc cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac
     9601 ttttgctttg ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac
     9661 tcagcaaaag ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct
     9721 gccagtgtta caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa
     9781 actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta
     9841 atgaaggaga aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg
     9901 cgattccgac tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt
     9961 tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcagct
    10021 tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca tcaaaatcac
    10081 tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga aatacgcgat
    10141 cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg aacactgcca
    10201 gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg aatgctgttt
    10261 tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata aaatgcttga
    10321 tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca tctgtaacat
    10381 cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg ggcttcccat
    10441 acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat ttatacccat
    10501 ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt tcccgttgaa
    10561 tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt attgttcatg
    10621 atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacacc gcgcttgcgg
    10681 aagcatcagc aaataaggcc agcacagcca gcgcagttgc cgctttggtt cctgattctg
    10741 ttcttgatga attaaacaaa gcggcacagt aacaaaggac ttcattgata atttttcttc
    10801 aggaggaaga catgtcattc ttttctacgt taaaaacagc tttgtctttg aaggagaaac
    10861 ttgctgctac tggtgttctt gttctgattt gcgcacttgt tggtgctggg tttgcatggg
    10921 aacgtcatca gctaaagcaa gccatagaga aaattggcag tcttgatcag gctgttaagg
    10981 aacgtgataa gtcaataatg gatcttaacc agaccattga gacgatgaac aaagcagagc
    11041 aacattttca cagccaggaa gtgaaaaatg aatcagaaca agccaagtat gctgacaggc
    11101 aaatggaacg aaaagctgaa gttcagaaac aactggttgc ggcgggtaat gttcgccagc
    11161 gtattcctgc tgacactcag cggttgctcc gggagtcgat cagcgaattt aacgccgacg
    11221 ccgacaaagg ttaaccaccc tgcccccaaa agtgcattta tgtgtaggat gccagagttt
    11281 agcagtgaat attttgatga tctgccagcg tatatcctcg atacagaaac gatgctgatg
    11341 gggattaaca ggaagaatcg caacgttaat gattacaacc gagctattag cggtaactaa
    11401 aagggatttt tatgtctgat aaagtaacag taaagcaaac tatcaacaaa gcgacttcaa
    11461 tctacaaaat tgagcaaatc actgttggca agccaggatc tgaacaatac cgtcgtgctt
    11521 tcgagcttgc cgatcagctt ggtttaaaac acccggattg cattgagcat gtatttccga
    11581 cctatgctga tgagcaatgt actcatgttc ttaccgaaga ggattttttc agcactgaag
    11641 aacgagaagg cgttgatcgc tgcattggtg tgatttgttc ttcggtaagt gatgagttat
    11701 tccctaatgt gcctgaatat ggtggtattg gataccaatt cctgtacgag ggcgatgagc
    11761 ttaaatgcta tgaacatggt cttctcatcg aaagcgtaga ataatacgac tcccttccaa
    11821 ccggctacgt tggccggttt ttcacttatc cacattatcc actggataga tccaataatc
    11881 aggtccatac agatcccaat tagatccata tagatccctg atcgttgcag gccgcgccac
    11941 gtctggctta gaagtgtatc gcgatgtgtg ctggagggaa aacgatgtgt gctggaggga
    12001 taaaaatgtg tgctgacggg ttgctaatgt gtgctggcgg gatataggat gtgtgttgac
    12061 gggaaagcta gcttgggtag ttatcaccac ttataaaaac tatccacaca attcggaaaa
    12121 agtaatatga atcaatcatt tatctccgat attctttacg cagacattga aagtaaggca
    12181 aaagaactaa cagttaattc aaacaacact gtgcagcctg tagcgttgat gcgcttgggg
    12241 gtattcgtgc cgaagccatc aaagagcaaa ggagaaagta aagagattga tgccaccaaa
    12301 gcgttttccc agctggagat agctaaagcc gagggttacg atgatattaa aatcaccggt
    12361 cctcgactcg atatggatac tgatttcaaa acgtggatcg gtgtcatcta cgcgttcagc
    12421 aaatacggct tgtcctcaaa caccatccag ttatcgtttc aggaattcgc taaagcctgt
    12481 ggtttcccct caaaacgtct ggatgcgaaa ctgcgtttaa ccattcatga atcacttgga
    12541 cgcttgcgta acaagggtat cgcttttaag cgcggaaaag atgctaaagg cggctatcag
    12601 actggtctgc tgaaggtcgg gcgttttgat gctgaccttg atctgataga gctggaggct
    12661 gattcgaagt tgtgggagct gttccagctt gattatcgcg ttctgttgca acaccacgcc
    12721 ttgcgtgccc ttccgaagaa agaagctgca caagccattt acactttcat cgaaagcctt
    12781 ccgcagaacc cgttgccgct atcgttcgcg cgaatccgtg agcgcctggc tttgcagtca
    12841 gctgttggcg agcaaaaccg tatcattaag aaagcgatag aacagcttaa aacaatcggc
    12901 tatctcgact gttctattga gaagaaaggc cgggaaagtt ttgtaatcgt ccattctcgc
    12961 aatccaaagc tgaaactccc cgaataagtg tgtgctggag ggaaaccgca ttaaaaagat
    13021 gtgtgctgcc gggaaggctt gtccaatttc ctgtttttga tgtgcgctgg agggggacgc
    13081 ccctcagttt gcccagactt tccctccagc acacatctgt ccatccgctt ttccctccag
    13141 cacacatcga agctgccggg caagccgttc tcaccagttg atagagagtg aagctagctt
    13201 ggctgcccat tgaagcagga aatcaccaaa atgattcagg ctacaacctg aacgtagaag
    13261 aaatccgcgt cctttatgcg tggaggatgc caaagcatgt tgtgacacac ttggcaaagg
    13321 agtaagcatg cagagaatgc tatgtacaag catctacgca tacattatta ttttatgcag
    13381 catttttaat taaattcaaa aatacagcat aaaggatgac tttcgatgag tgattccagc
    13441 cagcttcaca aggttgctca aagagcaaac agaatgctca atgttctgac tgaacaagta
    13501 cagttgcaaa aggatgagct acacgcgaac gagttttacc aggtctatgc gaaagcggca
    13561 ctggcaaaat tgcctctact gactcgagcg aacgttgact atgccgtaag tgaaatggaa
    13621 gaaaagggtt atgttttcga taaacgccct gctggctctt caatgaaata tgcgatgtca
    13681 attcagaaca tcattgacat atatgaacat cgcggagtgc caaaataccg ggatcgctac
    13741 agcgaagcgt atgtgatttt catctccaat cttaaaggcg gtgtgtcaaa aactgtatcg
    13801 acggtttctc tggcgcatgc aatgcgtgct caccctcatc ttcttatgga ggatttaagg
    13861 attctggtta ttgaccttga tccgcaatct tcagcaacga tgtttttaag ccataaacac
    13921 tctattggta tcgtaaacgc aacatctgca caggctatgt tgcagaatgt aagccgtgaa
    13981 gagctgttag aggagtttat tgttccttct gttgtacctg gggttgacgt tatgcctgcg
    14041 tcgattgacg atgcctttat tgcatccgat tggagagagc tgtgcaatga gcatctaccg
    14101 ggtcagaaca tccatgctgt cctgaaagaa aatgtgattg ataagctgaa gagcgattat
    14161 gactttatcc tcgttgatag tggtcctcac cttgacgcct tcctgaaaaa tgctttggcc
    14221 tcggccaata tactgtttac acctctgccg ccagcaactg tcgatttcca ctcatcgctt
    14281 aaatacgttg cccgccttcc tgagttggtg aaactcattt cggatgaagg ctgcgagtgc
    14341 cagcttgcga ctaacattgg ttttatgtcc aagttgagta acaaggcaga ccataagtat
    14401 tgccatagcc tggctaaaga agtgttcggt ggggatatgc ttgatgtctt cctccctcgc
    14461 cttgacggtt ttgaacgctg cggcgagtct tttgacactg ttatttcagc taacccggca
    14521 acgtatgttg gtagtgctga tgcattgaag aacgcgcgaa ttgccgcgga agattttgct
    14581 aaagcagttt ttgaccgtat tgaatttatc agatctaact gaggagtaag aaacccccat
    14641 gtcaaagaaa aacagaccaa caattgggcg aacccttaat ccttcaatat taagcggatt
    14701 tgatagttct tcagcctctg gcgatcgagt cgagcaggta ttcaagttat caactggtcg
    14761 ccaggccaca tttattgaag aggtaatacc tccgaaccag gtagaaagcg atacctttgt
    14821 tgatcagcat aacaacgggc gtgaccaggc atctcttacg ccaaaatcat taaaaagtat
    14881 ccgaagcact attaagcatc agcaatttta ccctgcaata ggtgttagac gggctacagg
    14941 gaaaattgaa attttggatg gttcccggcg tcgagcttct gccatcttag agaacgtagg
    15001 gttgcgggtt ttagtcacgg accaggagat cagcgttcag gaagcgcaaa atttagcgaa
    15061 agacgttcag acagcattgc agcacagcat tcgagaaata ggtctgcgtt tgatgcgaat
    15121 gaaaaatgat gggatgagtc agaaggatat tgcagccaaa gaagggctgt ctcaggcgaa
    15181 ggtcacgcgt gctctccagg cagcgagtgc tccggaagaa ttagtcgccc ttttccctgt
    15241 gcagtcggaa ttaacctttt cggactacaa aacgctttgt gctgttggcg acgaaatggg
    15301 gaacaagaat ttagagtttg atcagcttat tcaaaacata tccccggaaa taaacgacat
    15361 cttatccatt gaagaaatgg ccgaagatga agttaaaaat aaaatcctgc gcttgataac
    15421 aaaggaagcc tcactactca cggataaagg ttctaaagat aagtccgtag ttactgaatt
    15481 atggaaattt gaggacaagg atcgctttgc aaggaagcgc gtgaaaggcc gtgcattttc
    15541 ttatgagttt aatcgactct caaaagagtt acaggaagaa ctcgacagga tgattgggca
    15601 tatccttaga aagagcctcg ataaaaagcc gaagccttaa actttcgcca ttcaaatttc
    15661 actattaact gactgttttt aaagtaaatt actctaaaat ttcaaggtga aatcgccacg
    15721 atttcacctt ggattttacc ttcctcccct cctcccgaaa aaaataaaaa aattgcttgt
    15781 cacgagaaag tcaacaagtg actttcaata aaatctcttc cgaaaaggga ttcacacaag
    15841 tgccttgtgt ttaaggaaga gtaaattgag taacttacgc gaataccaga atcgtattgc
    15901 agatatcgca aaacgctcta aagctgtgct tggctgggca agcactgcgc agttcggtac
    15961 tgataaccaa ttcattaaag atgatgccgc gcgtgccgca tctatccttg aagctgcacg
    16021 taaagacccg gtttttgcgg gtatctctga taatgccacc gctcaaatcg ctacagcgtg
    16081 ggcaagtgca ctggctgact acgccgcagc acataaatct atgccgcgtc cggaaattct
    16141 ggcctcctgc caccagacgc tggaaaactg cctgatagag tccacccgca atagcatgga
    16201 tgccactaat aaagcgatgc tggaatctgt cgcagcagag atgatgagcg tttctgacgg
    16261 tgttatgcgt ctgcctttat tcctcgcgat gatcctgcct gttcagttgg gggcagctac
    16321 cgctgatgcg tgtaccttca ttccggttac gcgtgaccag tccgacatct atgaagtctt
    16381 taacgtggca ggttcatctt ttggttctta tgctgctggt gatgttctgg acatgcaatc
    16441 cgtcggtgtg tacagccagt tacgtcgccg ctatgtgctg gtggcaagct ccgatggcac
    16501 cagcaaaacc gcaaccttca agatggaaga cttcgaaggc cagaatgtac caatccgaaa
    16561 aggtcgcact aacatctacg ttaaccgtat taagtctgtt gttgataacg gttccggcag
    16621 cctacttcac tcgtttacta atgctgctgg tgagcaaatc actgttacct gctctctgaa
    16681 ctacaacatt ggtcagattg ccctgtcgtt ctccaaagcg ccggataaag gcactgagat
    16741 cgcaattgag acggaaatca atattgaagc cgctcctgag ctgatcccgc tgatcaacca
    16801 cgaaatgaag aaatacaccc tgttcccaag tcagttcgtt atcgcggctg agcacacggt
    16861 acaggcggcg tatgaagcac attattgaag catttatcag ggttattgtc tcatgagcgg
    16921 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
    16981 aaaagtgcca cctgtaatgt gagttagctc actcattagg caccccaggc tttacacttt
    17041 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
    17101 agctatgacc atgattacgc caagctagct tgcatgcctg caggtcgaag gccgtggcgg
    17161 cggcactttt gcccatgaat tgctcgctgt ggagtacgca ggctggattt ctcccgcgtt
    17221 tcggctgaag gtaaaccaga catttatcga ctatcgaacc ggaagattac aacctgctat
    17281 tccgcagagt ctccctgaag ctctccgttt ggctgccgac ctggcagagc aaaagcaacg
    17341 gctggagcaa aaaatgctta tggatgcacc taaagtcgaa ttcgctgaac gcgttgctac
    17401 cgccagcggg gttctaatcg gcaactatgc caaagtgctc ggcctgggcc aaaactatct
    17461 cttcacctgg ttgcgtgata acggaattct gatcgcaacc ggtgaacgca ggaacgtccc
    17521 caaacaagaa tacatatctc gtgggtattt cacccttaaa gaaaccgtga tcgatacaag
    17581 caatggaagc aggatttctt tcacgactcg tataaccggc aaaggtcagc agtggctgat
    17641 gaagcgattg cttgatgctg gtgtgctgct gtcgcggcaa cgcgctaaca gacgtagtaa
    17701 gaaccaccag cattgtaatg ctggctaaag tcactttcct gagctgtata acgatgagcg
    17761 attttacttt ttctggctat gaattggcct gctttgtaac acactccggt ctatcccgta
    17821 gcgccgggca tatcctgtcg caatgtgcaa atctcgcggc aacaaccagt gaatacttca
    17881 ttcacaagcc tcaccgcctg atcgcggcag aaactggtta tagccaatca accgtcgttc
    17941 gtgcattccg tgaagctgta aacaaaggaa ttctgtctgt agagattgtt atcggcgatc
    18001 accgtgaacg tcgcgctaac ctgtaccggt ttacaccatc ctttttggcc ttcgcacaac
    18061 aagccaaaaa tgcgctgata gaaagcaaat taaagatctc ttcagcggca accaaggtta
    18121 aagctgttct cgctaagaca ttggctttat ttaatttttt atccacaccc ccatgtcaaa
    18181 atgatacccc ctccccctgt caggatgacg tggcaataaa gaataagaag tcacaagtta
    18241 aaaaaacaaa aagatcagtt tccggcggtg ccggaacaac cagcctcaaa aaattgactt
    18301 catggatcgc taaggcaaaa gcaaaggctg acaatctgcg gttatccaaa aaacgcactc
    18361 aaaaacatga gttcaagcag aaagtagagg cggctgcgcg gaaatatgct tacctgaaga
    18421 acaagcgttc gcctgatatt ggcgggatat caaacttcga taacctaccg cattgcatga
    18481 cggtaaacga agctcttaat gcggttttag ccaaaaataa agataacgaa caatggggta
    18541 taccggcagg attcagaggg taatgaattg ctctaattat aaccatgcat actttcaaca
    18601 cctctagttt gccatgaggc aaactcatag gtgtcctggt aagaggacac tgttgccaaa
    18661 actggacgcc ccattattgc aattactaag aaaccattat tatcatgaca ttaacctata
    18721 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt ttataaaccg tggagcgggc
    18781 aatactgagc tgatgagcaa tttccgttgc accagtgccc ttctgatgaa gcgtcagcac
    18841 gacgttcctg tccacggtac gcctgcggcc aaatttgatt cctttcagct
//
