LOCUS       sequence-486050-        8610 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Control circuit for Re-NF controller.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8610)
  AUTHORS   Zhang R, Zhang R, Tian XJ
  TITLE     Integrating recombinase-based feedback and feedforward control for 
            optimal resource decoupling.
  JOURNAL   Nucleic Acids Res. 2025 Sep 5;53(17):gkaf913. doi: 
            10.1093/nar/gkaf913.
  PUBMED    40966512
REFERENCE   2  (bases 1 to 8610)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-09-5"; volume: "53(17):gkaf913. doi"; 
            pages: " 10.1093/nar/gkaf913"
COMMENT     Sequence Label: Circuit C52
FEATURES             Location/Qualifiers
     source          1..8610
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..35
                     /label=BBa_J23106
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.47"
     RBS             44..55
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             62..775
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(92..120)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     715..735
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     terminator      798..869
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      885..912
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        927..961
                     /label=BBa_J23110
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.33"
     RBS             970..981
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             988..1866
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     terminator      1888..1959
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1975..2002
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     promoter        2017..2051
                     /label=BBa_J23105
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.24"
     CDS             2080..3582
                     /codon_start=1
                     /transl_table=11
                     /gene="Bxb1"
                     /product="Bxb1 integrase"
                     /label=Bxb1 integrase
                     /note="Bxb1 large serine integrase from Mycobacterium 
                     bacteriophage Bxb1 (Ghosh et al. 2003)."
                     /protein_id="AFF61396.1"
                     /translation="MRALVVIRLSRVTDATTSPERQLEYCQQLCAQRGWDVVGVAEDLD
                     VSGAVDPFDRKRRPNLARWLAFEEQPFDVIVAYRVDRLTRSIRHLQQLVHWAEDHKKLV
                     VSATEAHFDTTTPFAAVVIALMGTVAQMELEAIKERNRSAAHFNIRAGKYRGSLPPWGY
                     LPTRVDGEWRLVPDPVQRERILEVYHRVVDNHEPLHLVAHDLNRRGVLSPKDYFAQLQG
                     REPQGREWSATALKRSMISEAMLGYATLNGKTVRDDDGAPLVRAEPILTREQLEALRAE
                     LVKTSRAKPAVSTPSLLLRVLFCAVCGEPAYKFAGGGRKHPRYRCRSMGFPKHCGNGTV
                     AMAEWDAFCEEQVLDLLGDAERLEKVWVAGSDSAVELAEVNAELVDLTSLVGSPAYRAG
                     SPQREALDARIAALAARQEELEGLEARPSGWEWRETGQRFGDWWREQDTAAKNTWLRSM
                     NVRLTFDVRGGLTRTIDFGDLQEYEQHLRLGSVVERLHTGMS"
     terminator      3601..3672
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3688..3715
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     RBS             4561..4572
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             complement(4584..5261)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPYDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             5268..5279
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      5628..5698
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5714..5741
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      5818..5889
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      5905..5932
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    5939..5959
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     CDS             complement(6099..6914)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(6099..6914)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     6822..6841
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     6906..6925
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      complement(7509..8053)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(7588..7607)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8521..8539
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(8543..8586)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    8589..8610
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 tttacggcta gctcagtcct aggtatagtg ctagctacta gagaaagagg agaaatacta
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacccttgt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 atactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      841 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg
      901 ggcctttctg cgtttatata ctagagttta cggctagctc agtcctaggt acaatgctag
      961 ctactagaga aagaggagaa atactagatg gctgaagcgc aaaatgatcc cctgctgccg
     1021 ggatactcgt ttaacgccca tctggtggcg ggtttaacgc cgattgaggc caatggttat
     1081 ctcgattttt ttatcgaccg accgctggga atgaaaggtt atattctcaa tctcaccatt
     1141 cgcggtcagg gggtggtgaa aaatcaggga cgagaatttg tctgccgacc gggtgatatt
     1201 ttgctgttcc cgccaggaga gattcatcac tacggtcgtc atccggaggc tcgcgaatgg
     1261 tatcaccagt gggtttactt tcgtccgcgc gcctactggc atgaatggct taactggccg
     1321 tcaatatttg ccaatacggg tttctttcgc ccggatgaag cgcaccagcc gcatttcagc
     1381 gacctgtttg ggcaaatcat taacgccggg caaggggaag ggcgctattc ggagctgctg
     1441 gcgataaatc tgcttgagca attgttactg cggcgcatgg aagcgattaa cgagtcgctc
     1501 catccaccga tggataatcg ggtacgcgag gcttgtcagt acatcagcga tcacctggca
     1561 gacagcaatt ttgatatcgc cagcgtcgca cagcatgttt gcctgtcgcc gtcgcgtctg
     1621 tcacatcttt tccgccagca gttagggatt agcgtcttaa gctggcgcga ggaccaacgc
     1681 atcagccagg cgaagctgct tttgagcact acccggatgc ctatcgccac cgtcggtcgc
     1741 aatgttggtt ttgacgatca actctatttc tcgcgagtat ttaaaaaatg caccggggcc
     1801 agcccgagcg agttccgtgc cggttgtgaa gaaaaagtga atgatgtagc cgtcaagttg
     1861 tcataataaa ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     1921 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     1981 ccttcgggtg ggcctttctg cgtttatata ctagagttta cggctagctc agtcctaggt
     2041 actatgctag ctactagagt cacacaggaa acctactaga tgagagccct ggtagtcatc
     2101 cgcctgtccc gcgtcaccga tgctacgact tcaccggagc gtcagctgga gtattgccag
     2161 cagctctgcg cccagcgcgg ctgggacgtc gtcggggtag cggaggatct ggacgtctcc
     2221 ggggcggtcg atccgttcga ccggaagcgc agaccgaacc tggcccggtg gctagcgttc
     2281 gaggagcaac cgtttgacgt gatcgtggcg taccgggtag atcggttgac ccgatcgatc
     2341 cggcatcttc agcagctggt ccactgggcc gaggaccaca agaagctggt cgtctccgcg
     2401 accgaagcgc acttcgatac gacgacgccg tttgcggcgg tcgtcatcgc gcttatggga
     2461 acggtggcgc agatggaatt agaagcgatc aaagagcgga accgttcggc tgcgcatttc
     2521 aatatccgtg ccgggaaata ccgaggatcc ctgccgccgt ggggatacct gcctacgcgc
     2581 gtggacgggg agtggcggct ggtgccggac cctgtgcagc gagagcgcat cctcgaggtg
     2641 tatcaccgcg tcgtcgacaa ccacgagccg ctgcatctgg tggcccacga cctgaaccgg
     2701 cgtggtgtcc tgtcgccgaa ggactacttc gcgcagctgc aaggccgcga gccgcagggc
     2761 cgggagtggt cggctaccgc gctgaagcga tcgatgatct ccgaggcgat gctcgggtac
     2821 gcgactctga acggtaagac cgtccgagac gacgacggag ccccgctggt gcgggctgag
     2881 ccgatcctga cccgtgagca gctggaggcg ctgcgcgccg agctcgtgaa gacctcccgg
     2941 gcgaagcccg cggtgtctac cccgtcgctg ctgctgcggg tgttgttctg cgcggtgtgc
     3001 ggggagcccg cgtacaagtt cgccggggga ggacgtaagc acccgcgcta ccgctgccgc
     3061 tcgatggggt tcccgaagca ctgcgggaac ggcacggtgg cgatggccga gtgggacgcg
     3121 ttctgcgagg agcaggtact ggatctgctc ggggacgcgg agcgtctgga gaaagtctgg
     3181 gtagcgggct cggactccgc ggtcgaactc gcggaggtga acgcggagct ggtggacctg
     3241 acgtcgctgg tcggctcccc ggcctaccgg gcgggctctc cgcagcgaga agcactggat
     3301 gcccgtattg cggcgctggc cgcgcggcaa gaggagctgg agggcctgga ggctcgcccg
     3361 tctggctggg agtggcgcga gaccgggcag cggttcgggg actggtggcg ggagcaggac
     3421 accgcggcaa agaacacctg gcttcggtcg atgaacgttc ggctgacgtt cgacgtccgc
     3481 ggcgggctga ctcgcacgat cgacttcggg gatcttcagg agtacgagca gcatctcagg
     3541 ctcggcagcg tggtcgaacg gctacacacc gggatgtcgt aataaactag agccaggcat
     3601 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     3661 gtgaacgctc tctactagag tcacactggc tcaccttcgg gttggccttt ctgcgtttat
     3721 atactagagg cgaaaaaacc ccgccgaagc ggggtttttt gcgtactaga gttattaacc
     3781 ggtgacttcc cagacctggc cgtcaacggt gaacttgcct cccaggatcg ggacgatctc
     3841 agccttgaca tgcttgccgt cgaccgtgag catcccgaag cccatctgcc agttcccggc
     3901 tccgcccttt agatagttgg ccttcttcat gtccatcagg tgcccgactt ccatgccggt
     3961 gacggtcttg cgcaccgagc cgccgtaccc gaacgagtgc gagacgacag cctgccggtg
     4021 cgtgtggccg cagaccacgg acttgccgaa cttcttggca ccgttgagcg ctgtcgatcc
     4081 ggcgatctgg gatagcgtca tcttgcccat gtgcccgtga gtggagatcc agcccggagc
     4141 gatgtcgtag aagtcaggca gcagctccac accgaacccg tcgaagtcga gcagcacgtc
     4201 gatgtcgaaa gcgtgcgtac cctccagggc cggtgcgttc ttggccaggt actcgcgggc
     4261 tcgcagatcg tggttgccct cgtgcatccc gatccagccg tcgtagacct tgcgcagcgg
     4321 ctccatcagg ttcttcttgg cgtagtccgc gtcgcggtac accgagccct cgaactcgcc
     4381 cttggttccc cggttccagc gcgagggctg cggcaggtct aggacgtcac cgatatgtac
     4441 cacgccgtac ggctggacat ccccgatgaa gcggatgacc gcttgcatct ctttgcgcgc
     4501 ctcgaaaggc aactgagtat cgggtagaaa gacgatacgc tgagtgccgg ccatctagta
     4561 tttctcctct tttactagag ttattaagca ccggtggagt gacgaccttc agcacgttcg
     4621 tactgttcaa cgatggtgta gtcttcgttg tgggaggtga tgtccagttt gatgtcggtt
     4681 ttgtaagcac ccggcagctg aaccggtttt ttagccatgt aggtggtttt aacttcagcg
     4741 tcgtagtgac caccgtcttt cagtttcaga cgcattttga tttcaccttt cagagcaccg
     4801 tcttccgggt acatacgttc ggtggaagct tcccaaccca tggttttttt ctgcataacc
     4861 ggaccgtcgt acgggaagtt ggtaccacgc agtttaactt tgtagatgaa ctcaccgtct
     4921 tgcagggagg agtcctgggt aacggtaaca acaccaccgt cttcgaagtt cataacacgt
     4981 tcccatttga aaccttccgg gaaggacagt ttcaggtagt ccgggatgtc agccgggtgt
     5041 ttaacgtaag ctttggaacc gtactggaac tgcggggaca ggatgtccca agcgaacggc
     5101 agcggaccac ctttggtaac tttcagttta gcggtctggg taccttcgta cggacgacct
     5161 tcaccttcac cttcgatttc gaactcgtga ccgttaacgg aaccttccat acgaactttg
     5221 aaacgcatga actctttgat aacgtcttcg gaggaagcca tctagtattt ctcctctttt
     5281 actagagcgg ccggcttgtc gacgacggcg cactcagtgg tgtacggtac aaaccccgac
     5341 aatagcgagg aggtaaattg taagctttag ggaaacgaca tttactgacg cgactttctc
     5401 aaagctaggg tgttggcctg aactgtacgc ttggagttgt aggacagaca taaaagtatc
     5461 agatacgcac actaaccatc tgctagccca aaaaaacggt atggagaaac agtagagagt
     5521 tgcgataaaa agcgtcaggt aggatccgct aatcttatgg actatcttgc tatggcatag
     5581 caaagtgtga cgccgtgcaa ataatcaatg tctctagtac caggcatcaa ataaaacgaa
     5641 aggctcagtc gaaagactgg gctttcgttt tatctgttgt ttgtcggtga acgctctcta
     5701 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatagcc cggatgatcc
     5761 tgacgacgga gaccgcggtg gttgaccaga caaaccacga ctactagagc caggcatcaa
     5821 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     5881 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata
     5941 ctagtagcgg ccgctgcagg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct
     6001 gcggcgagcg gtatcagctc actcaaaggc ggtaatctcg agtcccgtca agtcagcgta
     6061 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
     6121 aaatgaaact gcaatttatt catatcagga ttatcgatac catatttttg aaaaagccgt
     6181 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
     6241 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
     6301 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
     6361 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
     6421 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
     6481 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
     6541 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
     6601 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
     6661 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
     6721 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     6781 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     6841 ccatataaat cagcatccat gttggaattt aatcgcggcc tggagcaaga cgtttcccgt
     6901 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     6961 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     7021 ctttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgca tcttcccgac
     7081 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggtc cacctacaac
     7141 aaagctctca tcaaccgtgg ctccctcact ttctggctgg atgatggggc gattcaggcc
     7201 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctagcg gagtgtatac
     7261 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     7321 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     7381 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     7441 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     7501 aagccgtttt tccataggct ccgccccctg acaagcatca cgaaatctga cgctcaaatc
     7561 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
     7621 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     7681 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     7741 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     7801 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     7861 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     7921 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     7981 caaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     8041 aaaacgatct caagaagatc atcttattaa ggggtctgac gctcagtgga acgaaaactc
     8101 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8161 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8221 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8281 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8341 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8401 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8461 tattccatgg tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     8521 aataggcgta tcacgaggca gaatttcaga taaaaaaaat ccttagcttt cgctaaggat
     8581 gatttctgga attcgcggcc gcttctagag
//
