LOCUS       sequence-486268-       10373 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses cytosolic low affinity ratiometric ATP sensor in 
            Dopaminergic Neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10373)
  TITLE     Neuromodulator control of energy reserves in dopaminergic neurons
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10373)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pLenti-THP-(cyto).iATPSnFR2.A95A.A119L.miRFP67...
FEATURES             Location/Qualifiers
     source          1..10373
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1010..1030
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        1058..1284
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             1285..1465
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1512..1637
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2130..2363
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2548..2592
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2859..2975
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     6144..6165
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6268..6289)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    7274..7862
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7327..7347)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7745..7756)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7949..8182
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8254..8375
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8291..8310)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8345..8364
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8415..8550
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8570..8589)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8571..8589)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8599..8616)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8599..8615)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8608..8630)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8757..9212
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8844..8863)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9054..9075
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9238..9342
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9343..9411,9412..10203)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9561..9580)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaattcaaa attttatcga taccggtgag gcctcttggg
     3001 attcttctca tcaaaaaggg catgggacac agctaaagcg tccagggctc ctctgtgccc
     3061 acagatgcct tagatcttgg cacaatgtag tcagccagct ccgtgtgtgt gtgtgtgtgt
     3121 gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtttgcat gtatctcaca
     3181 gacagtgcac aatggcctgg atgtgaacag aggcaagagt ctgggccagc agttgtctcc
     3241 caggagggtc caaagacatc gtattttcaa gtttaggcca ggtgctcact tgggtgagct
     3301 cagacacaga caaaggtctg gagagcacac attccccacc cccacccagc ttctatgcaa
     3361 gcacctccag ctgagacaag aaaacgaatt aaaaagcaat atttgtgtca gcgtaagaca
     3421 tttgccgaaa ggttaaatcc acactcgtgg tgctgcacag cagccccctg tgcaggattt
     3481 gttaggcaca gctccctcct accccgtgcc acctgagcaa atgccaggct gggtgggctg
     3541 gaaccagcct gggcttgcct caccctggaa tccccagcac cctccaaagg aggaccctgg
     3601 gagtgggcat agacgccctt caggtgtggg caacagcccc cagtcctcag gatgaaaggc
     3661 taaggtgcag ccagctctgc cttcacggtg ggaatgtctc tatgtgagcc ctttctgggc
     3721 tgtgaagaac gctctgagaa gggtcctggg accctggata ggccagagct gtgctgggca
     3781 tgtagagaca ggagtgggct aaagcagcaa aggcactgac caaggaagag ttcagagagg
     3841 agcgtggaat atggggaggg gttcatagta agagagagca ggcagtggag agtaaatagt
     3901 cactgagccg gggtttatgg ggtttgtagg agcttactca gagaaagtag atgagagatg
     3961 ccatgccagt ctgagtatca cagagcccca ggctctcctg ggaacggaac tgtgagggcc
     4021 agaaggtcag caagggaggt tagggagagt tccttttgta ctgactcagc atttatcctg
     4081 ctcccagggg gcaatggggg ccagtgaggg atgcagagca aggcagtgat gtggcaggca
     4141 gttcctgttg tgaaagagct gggaagggag cgggctgggc ctggtacgta cagcaggcca
     4201 tttctgaggg tccgagtgct gtctaggagg tgcagtgaga cttcagtgat cagccagaac
     4261 agaagctaag cggggtgggg actgcgagtt caggcttctg ggtcttgcaa atatccagaa
     4321 tgctaaatcc tcagaacccc agggtggcca ttttcagagt gggttttgtc ctttgggcac
     4381 ttgtgcagac tccaatatcc agagggataa ggatggtact cttcagtacc cttagtgaga
     4441 ggacactttt ctctgaaggg cttgaatgtg ccgagccatt acctgaagga aggaaatgac
     4501 tccagggaca taggatgggc ccagcacaac tcacctgcta cagagaaagg tcccctccct
     4561 ggtctcctta gagatcctgt ttccctggct gaggaagcta gggtggatct ttgtgtaagt
     4621 gggtgtggat gctaactgga aaacaaaagg tcacttactg ttagacctcg gggtaccatg
     4681 gaagagatga tcactgagtg tgcccttaca tggggaccag ctgagaatgg ggctaccact
     4741 agctcgagac catgatacag ggaataagtg tgcatttggg ggtagggagt ggctcagaat
     4801 actcttaacc aaagcagagg tttgctccca caggaaggtg aggtcagaag gccttaggga
     4861 gctgccaggg gctagggttg gcaccatctc ccaggctgtg tctttaagga gatgataatc
     4921 agagggatag aaccttgcaa aagtgggcca gtcttgggaa tactatagag gaatagcctt
     4981 ctggaacatt ctgtgtctca taggacctgc ctggggatcc agccccagtg ccagcacata
     5041 taccgactgg ggcagtgaat agatagtaca ctttgttaca tgggctgggg ggaacatggc
     5101 ccatgtcctg gaggggactt tatgacagac atccaaaaat ccagtgagag ggcttctaga
     5161 tttgtctcca aaggttatag ttctaacatg agcccttagg aaatccagca tggttctccc
     5221 tgtgtgccct ggtttggtta gagagctcta gcggtctcct gtcccacaga ataccagcca
     5281 gcccctgccc tacgtcgtgc ctcgggctga gggtgattca gaggcaggtg cctgtgacag
     5341 tggatgcaat tagatctaat gggacggagg cctttctcgt cgccctcgct ccatgcccac
     5401 ccccgcctcc ctcaggcaca gcaggcgtgg agaggatgcg caggaggtag gaggtggggg
     5461 acccagaggg gctttgacgt cagcctggcc tttaaagagg gcgcctgcct ggcgagggct
     5521 gtggagacag aactcgggac caccagagga attcgatatc aagctgctag cgtttaaacg
     5581 ggccctctag actcgagcgc caccatgaag actattcacg tgagtgtcgt aactcccgac
     5641 gggcctgtat atgaggatga cgttgaaatg gtgagcgtca aagcaaaaag tggcgagctc
     5701 ggtattctcc caggccacat tcccttgaaa gctcccctag agatcagtgc cgcacgcctg
     5761 aagaaagggg gcaaaacaca gtatatcgct gtgtcaggcg gcaacctcga agtgcggcct
     5821 gacaaggtga ccatctatgc ccaggcagcc gagagggctg aggatatcga tgtcctgcgc
     5881 gccaaggccg ccaaggagag agccgagcgc cgactccaat cacaggtcct ggtcgggaac
     5941 gtctatatca ccgccgacaa gcagaagaac ggcatcaagg cgaacttcaa gatccgccac
     6001 aacgtggagg acggcagcat gcagctcgcc gaccactacc agcagaacac ccccatcggc
     6061 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgt gctgagcaaa
     6121 gaccctaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     6181 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatgag caagggcgag
     6241 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     6301 aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
     6361 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     6421 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     6481 tccgccatgc ccgaaggcta cgtccaggag cgcaccatca gcttcaagga cgacggcacc
     6541 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     6601 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacttt
     6661 atttgtgtgg acatcgattt taagcgcgcc gagctcttgc ttaagcgcgc aatgaatagg
     6721 ctctcagttg ccgaaatgaa gctgcagggc ggaggctcgg gcggaggtgg gtcgggtggc
     6781 ggcggatcaa tggcaaacct ggacaagatg ctgaacacca ccgtgaccga ggtgcgcaag
     6841 ttcctgcaag cggacagagt gtgcgtgttc aagttcgagg aagattactc cggcaccgtc
     6901 tcgcacgaag ccgtggacga cagatggatt agcatcctga agacccaggt gcaggacaga
     6961 tacttcatgg aaaccagagg cgaggaatac gtccacggca gataccaggc catcgccgac
     7021 atctacacag ccaatctggt cgagtgctac agagacctgc tgatcgagtt tcaggtgcgg
     7081 gccattctgg ctgtccccat cctgcaaggc aagaagctgt ggggcctgct ggtggcccac
     7141 caactggccg gccctcggga gtggcagacc tgggaaatcg acttcctgaa acagcaagcc
     7201 gtggtgatgg gcatcgccat ccagcagagc tagggtcgac tccggatgat cagggccctg
     7261 tacagatatc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     7321 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7381 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7441 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7501 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7561 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7621 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7681 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7741 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7801 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7861 gcctggaatt cgagctcggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     7921 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     7981 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     8041 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     8101 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     8161 tcagtgtgga aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa
     8221 cttgcaaaga aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt
     8281 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8341 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc
     8401 ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg
     8461 gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc
     8521 agaagtagtg aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga
     8581 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg
     8641 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga
     8701 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc
     8761 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8821 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8881 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8941 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     9001 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9061 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9121 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9181 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc
     9241 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     9301 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     9361 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     9421 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     9481 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     9541 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     9601 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     9661 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     9721 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     9781 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     9841 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     9901 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     9961 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
    10021 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
    10081 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
    10141 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
    10201 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
    10261 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
    10321 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttc
//
