LOCUS       sequence-486270-       10845 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of cleavable Trp1 (extracellular domain).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10845)
  AUTHORS   van der Plas – van Duijn A, Winkelman L, Lensen C, van Duivendijk M,
            Derksen I, van Veenendaal J, Schaap G, Sluijter M, van Groningen T, 
            van Doorn R, van Hall T, Slieker R, Sharp T, Beltman J, Bresser K, 
            Scheeren F
  TITLE     Pyroglutamation of cell surface proteins CD47 and TRP1 by glutaminyl
            cyclase modulates therapeutic antibody binding.
  JOURNAL   Commun Biol 8, 1561 (2025).
REFERENCE   2  (bases 1 to 10845)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun Biol
            8, 1561 (2025)."
COMMENT     Sequence Label: cleaveBADTrp
FEATURES             Location/Qualifiers
     source          1..10845
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1453..1476
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             1540..1569
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    2989..3577
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3042..3062)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3460..3471)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3649..3882
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3960..4081
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3997..4016)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4051..4070
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4121..4256
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4276..4295)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4277..4295)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4305..4322)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4305..4321)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4314..4336)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4463..4918
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4550..4569)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4760..4781
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4944..5048
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5049..5117,5118..5909)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5267..5286)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6080..6668
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6569..6588
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6822..6839
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6956..6977
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6992..6997,6998..7015,7016..7022)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7030..7046
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7035..7057
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7054..7070
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7054..7070
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7089..7109
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7091..7109
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7137..7363
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7364..7544
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7591..7716
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8209..8442
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8627..8671
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8846..9346
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     9265..9283
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             9368..9967
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(9368..9387)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9864..9884
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10009..10126
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        10243..10622
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10623..10826
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10777..10797
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 atgtctgccc ccaaacttct gtcactcgga tgcatatttt ttccactctt actgtttcag
       61 caggcgaggg cccaattccc caggcagtgt gccactgtag aggcactgcg cagcggcatg
      121 tgttgccccg acttatcccc tgtttctggc ccgggcaccg accgctgcgg aagttcctct
      181 ggtagaggcc ggtgcgaagc tgtgacggcc gattcccgcc cacattcccc gcagtatccg
      241 cacgatggcc gtgatgaccg ggaggtttgg cctcttcggt ttttcaaccg gacttgccac
      301 tgtaacggga actttagcgg tcataattgt ggcacatgca gaccaggttg gcgaggggcc
      361 gcttgtgacc agcgggtcct cattgtacgt cggaacctgc ttgacttgtc taaggaggaa
      421 aaaaaccact tcgtcagagc actcgacatg gcaaaaagga ccacacatcc actttttgtt
      481 attgcaactc gccgctcaga agaaattctg ggccctgatg gcaatacacc acagtttgag
      541 aacatcagca tctataacta cttcgtgtgg acgcattatt actccgtcaa gaagaccttc
      601 cttggagtgg gacaggagtc cttcggagag gtggattttt cacacgaagg cccagctttt
      661 ttgacttggc atcgttacca cctcctcagg cttgagaagg atatgcagga gatgttgcag
      721 gagccatctt tctccttacc ttactggaat ttcgctaccg gcaaaaacgt ctgcgatatc
      781 tgcaccgacg accttatggg ttccaggtcc aattttgatt ctactctcat ttcccctaat
      841 tcagtgtttt ctcagtggcg ggtggtatgc gattccctgg aggactatga cacactcggg
      901 actctctgta atagtaccga ggacggaccc attcgccgga atcctgcggg gaacgtagcc
      961 cgaccaatgg ttcagcggct tcccgaacca caagacgttg cccagtgcct ggaagtgggc
     1021 ctgttcgaca ctcctccgtt ttacagtaac tccacgaata gctttaggaa caccgtcgag
     1081 gggtactccg atccgactgg gaagtatgac cctgcagtca ggagcctcca caatcttgcc
     1141 cacctgtttt tgaacggcac tggtgggcag acgcacttat ccccgaacga tccgatcttc
     1201 gtgctgcttc atacctttac cgatgctgtg ttcgacgaat ggttgaggag atataatgcc
     1261 gacatttcca catttcctct ggagaatgcc cccatcggtc acaacagaca gtataacatg
     1321 gttccattct ggccacctgt caccaatacc gagatgtttg ttacagcacc tgataacctc
     1381 ggatacactt acgagattca gtggcctggc ggctccggcg gcagtggggg ctctggcgga
     1441 tcaggcggaa gccatcatca ccaccaccac catcatggcg gctccggggg cagcgatgag
     1501 atggaggagt gtagccagca tggcggaagc gggggttctg agcagaaact gatcagcgaa
     1561 gaagacctga ccggttgtgt agtcatagtg gggcgcatcg tgctctccgg tagcggaaca
     1621 agtgccccaa tcacggccta tgcacagcag actcgggggc tgctgggttg catcatcaca
     1681 tccttgacgg ggagggataa aaaccaggtg gagggtgaag tccagattat gagcactgct
     1741 acgcaaacct tcttagccac atgtatcaat ggggtttgtt gggcagtcta tcatggtgca
     1801 gggacaagaa ccatcgccag tcccaaggga ccagtaatcc aaatgtacac caatgtggat
     1861 caggacctcg tgggatggcc ggcgccccaa ggatccagat ccctcacccc ttgtacttgc
     1921 gggagttctg acctgtacct ggtcacaaga cacgcggacg tgatccctgt cagacgcaga
     1981 ggagacgggc gaggctcact gcttagtccg aggcccatct cttatctcaa ggggagctcc
     2041 ggcggccccc tgctttgtcc tgccggacat gctgtcggtt tgttccgagc cgccgtgtgt
     2101 accagagggg ttgcaaaagc cgtagatttt atccccgtgg aaaatctgga aaccaccatg
     2161 agaagccctg tgttcacaga taactccagc ccgccagccg tgacactcac tcatgggggt
     2221 tcaggaggct ctgggtgcat tggggccgcc acaactaccc cagcgcccag acctcctacg
     2281 cctgcgccca caatagcctc tcagcccctg agcttaagac ccgaagcctg tcgccccgcc
     2341 gcaggtgggg ctgtgcacac ccggggtctc gattttgcct gcgacattta tatatgggca
     2401 ccattggcag gcacctgcgg ggtgttattg ctcagcttgg tcataacttt atattgcaaa
     2461 agagggcgca aaaagctgct ctacatcttc aagcagccat tcatgcgacc cgtgcaaaca
     2521 acgcaggaag aagacggctg tagctgcaga ttccctgagg aagaggaagg tgggtgtgag
     2581 ctgagggtca aattctccag atctgctgac gcacctgcct ataaacaggg ccagaatcag
     2641 ctttataatg agcttaactt aggcagacgc gaagaatatg acgtgctgga caagaggcgc
     2701 ggcagagatc cagagatggg tggcaaacct cgacggaaaa acccccagga aggcctgtac
     2761 aacgaacttc agaaggacaa aatggccgaa gcatacagcg agatcggaat gaagggggag
     2821 agacgccgcg gtaagggaca cgatgggctc tatcagggac tttctactgc taccaaggac
     2881 acatacgatg ccctccacat gcaggccctt ccccctcggt aggatatcca gcacagtggc
     2941 ggccgctcga gtctagaggg cccgtttaaa cccgctgatc agcctcgaaa tcaacctctg
     3001 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3061 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3121 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3181 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3241 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3301 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     3361 aattccgtgg tgttgtcggg gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc
     3421 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     3481 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     3541 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     3601 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     3661 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     3721 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     3781 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     3841 gatccctcag acccttttag tcagtgtgga aaatctctag cagtacgtat agtagttcat
     3901 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagagga
     3961 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     4021 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     4081 atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc taactccgcc
     4141 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     4201 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     4261 gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc cgtcgtttta
     4321 caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc agcacatccc
     4381 cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc ccaacagttg
     4441 cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     4501 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     4561 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     4621 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     4681 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     4741 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     4801 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     4861 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttag
     4921 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     4981 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5041 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5101 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5161 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5221 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5281 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5341 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5401 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5461 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     5521 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     5581 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     5641 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     5701 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     5761 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     5821 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     5881 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     5941 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6001 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6061 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6121 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6181 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6241 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6301 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6361 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6421 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6481 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     6541 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     6601 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     6661 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     6721 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
     6781 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
     6841 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     6901 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     6961 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     7021 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     7081 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
     7141 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
     7201 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
     7261 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
     7321 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
     7381 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     7441 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     7501 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
     7561 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
     7621 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
     7681 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
     7741 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
     7801 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
     7861 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
     7921 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
     7981 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
     8041 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
     8101 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
     8161 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
     8221 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
     8281 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
     8341 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
     8401 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
     8461 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
     8521 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
     8581 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
     8641 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
     8701 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
     8761 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
     8821 ccacctccca accccgaggg gacccttgcg ccttttccaa ggcagccctg ggtttgcgca
     8881 gggacgcggc tgctctgggc gtggttccgg gaaacgcagc ggcgccgacc ctgggtctcg
     8941 cacattcttc acgtccgttc gcagcgtcac ccggatcttc gccgctaccc ttgtgggccc
     9001 cccggcgacg cttcctgctc cgcccctaag tcgggaaggt tccttgcggt tcgcggcgtg
     9061 ccggacgtga caaacggaag ccgcacgtct cactagtacc ctcgcagacg gacagcgcca
     9121 gggagcaatg gcagcgcgcc gaccgcgatg ggctgtggcc aatagcggct gctcagcagg
     9181 gcgcgccgag agcagcggcc gggaaggggc ggtgcgggag gcggggtgtg gggcggtagt
     9241 gtgggccctg ttcctgcccg cgcggtgttc cgcattctgc aagcctccgg agcgcacgtc
     9301 ggcagtcggc tccctcgttg accgaatcac cgacctctct ccccaggggg taccaccgga
     9361 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
     9421 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
     9481 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
     9541 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
     9601 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
     9661 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
     9721 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
     9781 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
     9841 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
     9901 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
     9961 tgcctgagaa ttctagatct tgagacaaat ggcagtattc atccacaatt ttaaaagaaa
    10021 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
    10081 acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag
    10141 ggacagcaga gatccacttt ggcgccggct cgagggggcc cggggttatt aatagtaatc
    10201 aattacgggg tcattagttc atagcccata tatggagttc cggacattga ttattgacta
    10261 gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg
    10321 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
    10381 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
    10441 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
    10501 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
    10561 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
    10621 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
    10681 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
    10741 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
    10801 ggtgggaggt ctatataagc agagctgaga cccaagctgg ctagc
//
