LOCUS       sequence-486285-        7394 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER targeted expression of green glucose sensor with non-responsive 
            HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7394)
  AUTHORS   Marvin JS, Machler P, Meng C, Ates T, Patel RH, Adhikari R, Makurath
            MA, Ku Z, Feliciano D, Atasoy D, Cui G, Kleinfeld D, Brown TA
  TITLE     iGlucoSnFR2: A genetically encoded fluorescent sensor for measuring 
            intracellular or extracellular glucose in vivo in mouse brain.
  JOURNAL   Sci Adv. 2025 Nov 14;11(46):eadz3889. doi: 10.1126/sciadv.adz3889. 
            Epub 2025 Nov 12.
  PUBMED    41223288
REFERENCE   2  (bases 1 to 7394)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-11-14"; volume: "11(46):eadz3889. doi"; pages: " 
            10.1126/sciadv.adz3889. Epub 2025 Nov 12"
COMMENT     Sequence Label: pAAV.GFAP.(ER).iGlucoSnFR2.HaloTag
FEATURES             Location/Qualifiers
     source          1..7394
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(17..472)
                     /direction=LEFT
                     /label=M13 ori
                     /note="M13 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(154..175)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     366..385
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   544..673
                     /label=AAV2 ITR
     primer_bind     2688..2709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(2812..2833)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    4396..4984
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4449..4469)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4867..4878)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    5012..5133
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5023..5042)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5077..5096
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   5295..5424
                     /label=AAV2 ITR
     rep_origin      complement(5661..6249)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5741..5760)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6420..7211,7212..7280))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7281..7385)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
       61 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
      121 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
      181 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
      241 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
      301 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
      361 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
      421 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
      481 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
      541 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
      601 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      661 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      721 tctaggaaga tcgtaggtta attaataaca tatcctggtg tggagtaggg gacgctgctc
      781 tgacagaggc tcgggggcct gagctggctc tgtgagctgg ggaggaggca gacagccagg
      841 cctttgtctg caagcagacc tggcagcatt gggctggccg ccccccaggg cctcctcttc
      901 atgcccagtg aatgactcac cttggcacag acacaatgtt cggggtgggc acagtgcctg
      961 cttcccgccg caccccagcc cccctcaaat gccttccgag aagcccattg agcagggggc
     1021 ttgcattgca ccccagcctg acagcctggc atcttgggat aaaagcagca cagcccccta
     1081 ggggctgccc ttgctgtgtg gcgccaccgg cggtggagaa caaggctcta ttcagcctgt
     1141 gcccaggaaa ggggatcagg ggatgcccag gcatggacag tgggtggcag ggggggagag
     1201 gagggctgtc tgcttcccag aagtccaagg acacaaatgg gtgaggggag agctctcccc
     1261 atagctgggc tgcggcccaa ccccaccccc tcaggctatg ccagggggtg ttgccagggg
     1321 cacccgggca tcgccagtct agcccactcc ttcataaagc cctcgcatcc caggagcgag
     1381 cagagccaga gcaggttgga gaggagacgc atcacctccg ctgctcgcgg ggatcccgcc
     1441 accatgctgc tatccgtgcc gctgctgctc ggcctcctcg gcctggccat cgccagatct
     1501 aagctggaga ttttctcttg gtgggctggg gatgaaggcc ctgccctgga ggcactcatc
     1561 agactgtata aacagaagta tcctggagta gaggtgataa acgccactgt caccggaggt
     1621 gccggtgtaa atgctcgcgc tgtccttaag acaaggatgc ttggtggtga tcctcctgat
     1681 acatttcaag ttgcagcagg gatggagttg ataggcacat gggtggtggc taacagaatg
     1741 gaggatctca gcgccttgtt tcgacaagag ggttggcttc aagccttccc caagggcctt
     1801 attgatctga ttagttataa aggtggtata tggagtgtgc ccgttaatat tcacaggtca
     1861 aacgtaatgt ggtatttgcc tgcaaagcta aaagagtggg gagttaaccc cccacggact
     1921 tgggatgagt ttcttgccac ctgtcaaaca cttaaacaaa agggtctgga agctccactg
     1981 gctctcgggg aaaattggac acaacaacac ctctgggaga gtgtcgcctt ggctgtattg
     2041 gggcctgatg attggaataa tctgtggaat gggaagctga agttcacaga ccctaaagca
     2101 gttagggcat gggaagtctt tggccgcgtt ttggactgtg ccaacaagga tgcagcaggc
     2161 ttgtcttggc agcaagctgt cgatcgggta gtacagggga aagccgcttt taacgtaatg
     2221 ggcgactggg cagcaggata tatgactacc actctcaagt tgaagcctgg tacagatttc
     2281 gcatgggcac caagtcccgg gacacagggc gtctttatga tggtttccga ctctttcgga
     2341 cttcccaaag gagccaaaaa tcgacaaaac gctatcaact ggcttcgatt ggtcggctct
     2401 aaagaaggtc aagatacatt caacccactg aaagggtcca tcgctgctcg gctcgatagc
     2461 gacccaagca agtatccgtg taacgtctat atcaccgccg acaagcagaa gaacggcatc
     2521 aaggcgaact tcaagatccg ccacaacgtg gaggacggca gcgtgcagct cgccgaccac
     2581 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2641 gtgacccagt ccgtgctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2701 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gggcggtacc
     2761 ggagggagca tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     2821 ctggacggcg acgtaaacgg ccacaagttc agcgtgcgcg gcgagggcga gggcgatgcc
     2881 accaacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     2941 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3001 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3061 atcagcttca aggacgacgg cacctacaag acccgcgccg aggtgaagtt cgagggcgac
     3121 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3181 gggcacaagc tggagtacaa ctttaacaac cccaacgcat acggacagag tgcaatgcga
     3241 gattggcgga gtaatcgcat cgttggctcc ctggtagcag gagcagtggc ccctgaaagc
     3301 tccatgtcac aattcggaac agtaatggaa attttcctgc aaacacgcaa cccacaagca
     3361 gcagcaaatg cagcacaagc tattgctgac caagttggcc tgggtcgcct tggtcagctg
     3421 cagggcggag gctcgggcgg aggtgggtcg ggtggcggcg gatcaatggc cgagatcggc
     3481 accggcttcc ccttcgaccc ccactacgtg gaggtgctgg gcgagcgcat gcactacgtt
     3541 gacgtgggcc cccgcgacgg cacccccgtg ctgttcctgc acggcaaccc caccagcagc
     3601 tacgtgtggc gcaacatcat cccccacgtg gcccccaccc accgcactat cgcccccgac
     3661 ctgatcggca tgggcaagag cgacaagccc gacctgggct acttcttcga cgaccacgtg
     3721 cgcttcatgg acgccttcat cgaggccctg ggcctggagg aggtggtgct ggtgatccac
     3781 gactggggca gcgccctggg cttccactgg gccaagcgca accccgagcg cgtgaagggc
     3841 atcgccttca tggagttcat ccgccccatc cccacctggg acgagtggcc cgagttcgcc
     3901 cgcgagacct tccaggcctt ccgcaccacc gacgtgggcc gcaagctgat catcgaccag
     3961 aacgtgttca tcgagggcac cctgcccatg ggcgtggtgc gccccctgac cgaggtggag
     4021 atggaccact accgcgagcc cttcctgaac cccgtggacc gcgagcccct gtggcgcttc
     4081 cccaacgagc tgcccatcgc cggcgagccc gccaacatcg tggccctggt ggaggagtac
     4141 atggactggc tgcaccagag ccccgtgccc aagctgctgt tctggggcac ccccggcgtg
     4201 ctgatccccc ccgccgaggc cgcccgcctg gccaagagcc tgcccaacgt taaggccgtg
     4261 gacatcggcc ccggcctgaa cctgctccag gaggacaacc ccgacctgat cggcagcgag
     4321 atcgcccgct ggctgagcac cctcgagatc agcggcaaag atgagttgta gaagcttttc
     4381 gaattagctt atcataatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4441 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4501 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4561 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4621 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4681 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4741 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4801 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4861 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     4921 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     4981 ccgcatcgga ggccgcttcg agcagacatg ataagataca ttgatgagtt tggacaaacc
     5041 acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta
     5101 tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg
     5161 tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt
     5221 ggtaaaatcg ataaggatct tcctagagca tggctacgta gataagtagc atggcgggtt
     5281 aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
     5341 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
     5401 ctcagtgagc gagcgagcgc gcagctgcat taatgaatcg gccaacgcgc ggggagaggc
     5461 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     5521 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     5581 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     5641 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     5701 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     5761 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     5821 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     5881 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     5941 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     6001 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     6061 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     6121 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     6181 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     6241 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     6301 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     6361 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     6421 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     6481 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     6541 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     6601 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     6661 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     6721 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     6781 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     6841 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     6901 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     6961 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     7021 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     7081 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     7141 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     7201 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     7261 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     7321 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     7381 ccgcgcacat ttcc
//
