LOCUS       sequence-486339-       12335 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AtACT2 promoter driving the expression of dCas9-ZAT10(2x).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12335)
  AUTHORS   Khan MA, Herring G, Zhu JY, Oliva M, Fourie E, Johnston B, Zhang Z, 
            Potter J, Pineda L, Pflueger J, Swain T, Pflueger C, Lloyd JPB, 
            Secco D, Small I, Kidd BN, Lister R
  TITLE     CRISPRi-based circuits to control gene expression in plants.
  JOURNAL   Nat Biotechnol. 2025 Mar;43(3):416-430. doi: 
            10.1038/s41587-024-02236-w. Epub 2024 May 20.
  PUBMED    38769424
REFERENCE   2  (bases 1 to 12335)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41587-024-02236-w"; journalName: "Nat Biotechnol"; date: 
            "2025-03"; volume: "43"; issue: "3"; pages: "416-430"
COMMENT     Sequence Label: LLP1020
FEATURES             Location/Qualifiers
     source          1..12335
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    433..457
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             1367..1387
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(1400..1423,1424..1426,1427..3913,3914..3916,
                     3917..5500)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             5513..5533
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             5540..5560
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    6458..6482
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             7783..8412
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             8841..9914
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      9980..10174
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(10410..10432)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    10518..10658
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     10573..10592
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(10673..10690)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10844..11432)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10924..10943)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(11520..12335)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(11520..12335)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     12243..12262
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 aacagaactt attatttcct tcctcttttc tacagtattt aaagataccc caagaagcta
       61 attataacaa gacgaactcc aattcactgt tccttgcatt ctaaaacctt aaataccaga
      121 aaacagcttt ttcaaagttg ttttcaaagt tggcgtataa catagtatcg acggagccga
      181 ttttgaaacc gcggtgatca caggcagcaa cgctctgtca tcgttacaat caacatgcta
      241 ccctccgcga gatcatccgt gtttcaaacc cggcagctta gttgccgttc ttccgaatag
      301 catcggtaac atgagcaaag tctgccgcct tacaacggct ctcccgctga cgccgtcccg
      361 gactgatggg ctgcctgtat cgagtggtga ttttgtgccg agctgccggt cggggagctg
      421 ttggctggct ggtggcagga tatattgtgg tgtaaacata acgaattgct cttcatacag
      481 gagtcgacaa aatttagaac gaacttaatt atgatctcaa atacattgat acatatctca
      541 tctagatcta ggttatcatt atgtaagaaa gttttgacga atatggcacg acaaaatggc
      601 tagactcgat gtaattggta tctcaactca acattatact tataccaaac attagttaga
      661 caaaatttaa acaactattt tttatgtatg caagagtcag catatgtata attgattcag
      721 aatcgttttg acgagttcgg atgtagtagt agccattatt taatgtacat actaatcgtg
      781 aatagtgaat atgatgaaac attgtatctt attgtataaa tatccataaa cacatcatga
      841 aagacacttt ctttcacggt ctgaattaat tatgatacaa ttctaataga aaacgaatta
      901 aattacgttg aattgtatga aatctaattg aacaagccaa ccacgacgac gactaacgtt
      961 gcctggattg actcggttta agttaaccac taaaaaaacg gagctgtcat gtaacacgcg
     1021 gatcgagcag gtcacagtca tgaagccatc aaagcaaaag aactaatcca agggctgaga
     1081 tgattaatta gtttaaaaat tagttaacac gagggaaaag gctgtctgac agccaggtca
     1141 cgttatcttt acctgtggtc gaaatgattc gtgtctgtcg attttaatta tttttttgaa
     1201 aggccgaaaa taaagttgta agagataaac ccgcctatat aaattcatat attttcctct
     1261 ccgctttgaa tactgtttat ttacaagagc gtacggttca acccctgcct cccctgtaaa
     1321 actccctaga cttaaatctt ttctttctag tatcttttca atgatgccaa agaagaagag
     1381 aaaggtcggg ggtggttctg ataagaagta ctctatcgga ctcgctatcg gaactaactc
     1441 tgtgggatgg gctgtgatca ccgatgagta caaggtgcca tctaagaagt tcaaggttct
     1501 cggaaacacc gataggcact ctatcaagaa aaaccttatc ggtgctctcc tcttcgattc
     1561 tggtgaaact gctgaggcta ccagactcaa gagaaccgct agaagaaggt acaccagaag
     1621 aaagaacagg atctgctacc tccaagagat cttctctaac gagatggcta aagtggatga
     1681 ttcattcttc cacaggctcg aagagtcatt cctcgtggaa gaagataaga agcacgagag
     1741 gcaccctatc ttcggaaaca tcgttgatga ggtggcatac cacgagaagt accctactat
     1801 ctaccacctc agaaagaagc tcgttgattc tactgataag gctgatctca ggctcatcta
     1861 cctcgctctc gctcacatga tcaagttcag aggacacttc ctcatcgagg gtgatctcaa
     1921 ccctgataac tctgatgtgg ataagttgtt catccagctc gtgcagacct acaaccagct
     1981 tttcgaagag aaccctatca acgcttcagg tgtggatgct aaggctatcc tctctgctag
     2041 gctctctaag tcaagaaggc ttgagaacct cattgctcag ctccctggtg agaagaagaa
     2101 cggacttttc ggaaacttga tcgctctctc tctcggactc acccctaact tcaagtctaa
     2161 cttcgatctc gctgaggatg caaagctcca gctctcaaag gatacctacg atgatgatct
     2221 cgataacctc ctcgctcaga tcggagatca gtacgctgat ttgttcctcg ctgctaagaa
     2281 cctctctgat gctatcctcc tcagtgatat cctcagagtg aacaccgaga tcaccaaggc
     2341 tccactctca gcttctatga tcaagagata cgatgagcac caccaggatc tcacacttct
     2401 caaggctctt gttagacagc agctcccaga gaagtacaaa gagattttct tcgatcagtc
     2461 taagaacgga tacgctggtt acatcgatgg tggtgcatct caagaagagt tctacaagtt
     2521 catcaagcct atcctcgaga agatggatgg aaccgaggaa ctcctcgtga agctcaatag
     2581 agaggatctt ctcagaaagc agaggacctt cgataacgga tctatccctc atcagatcca
     2641 cctcggagag ttgcacgcta tccttagaag gcaagaggat ttctacccat tcctcaagga
     2701 taacagggaa aagattgaga agattctcac cttcagaatc ccttactacg tgggacctct
     2761 cgctagagga aactcaagat tcgcttggat gaccagaaag tctgaggaaa ccatcacccc
     2821 ttggaacttc gaagaggtgg tggataaggg tgctagtgct cagtctttca tcgagaggat
     2881 gaccaacttc gataagaacc ttccaaacga gaaggtgctc cctaagcact ctttgctcta
     2941 cgagtacttc accgtgtaca acgagttgac caaggttaag tacgtgaccg agggaatgag
     3001 gaagcctgct tttttgtcag gtgagcaaaa gaaggctatc gttgatctct tgttcaagac
     3061 caacagaaag gtgaccgtga agcagctcaa agaggattac ttcaagaaaa tcgagtgctt
     3121 cgattcagtt gagatttctg gtgttgagga taggttcaac gcatctctcg gaacctacca
     3181 cgatctcctc aagatcatta aggataagga tttcttggat aacgaggaaa acgaggatat
     3241 cttggaggat atcgttctta ccctcaccct ctttgaagat agagagatga ttgaagaaag
     3301 gctcaagacc tacgctcatc tcttcgatga taaggtgatg aagcagttga agagaagaag
     3361 atacactggt tggggaaggc tctcaagaaa gctcattaac ggaatcaggg ataagcagtc
     3421 tggaaagaca atccttgatt tcctcaagtc tgatggattc gctaacagaa acttcatgca
     3481 gctcatccac gatgattctc tcacctttaa agaggatatc cagaaggctc aggtttcagg
     3541 acagggtgat agtctccatg agcatatcgc taacctcgct ggatctcctg caatcaagaa
     3601 gggaatcctc cagactgtga aggttgtgga tgagttggtg aaggtgatgg gaaggcataa
     3661 gcctgagaac atcgtgatcg aaatggctag agagaaccag accactcaga agggacagaa
     3721 gaactctagg gaaaggatga agaggatcga ggaaggtatc aaagagcttg gatctcagat
     3781 cctcaaagag caccctgttg agaacactca gctccagaat gagaagctct acctctacta
     3841 cctccagaac ggaagggata tgtatgtgga tcaagagttg gatatcaaca ggctctctga
     3901 ttacgatgtt gatgctatcg tgccacagtc attcttgaag gatgattcta tcgataacaa
     3961 ggtgctcacc aggtctgata agaacagggg taagagtgat aacgtgccaa gtgaagaggt
     4021 tgtgaagaaa atgaagaact attggaggca gctcctcaac gctaagctca tcactcagag
     4081 aaagttcgat aacttgacta aggctgagag gggaggactc tctgaattgg ataaggcagg
     4141 attcatcaag aggcagcttg tggaaaccag gcagatcact aagcacgttg cacagatcct
     4201 cgattctagg atgaacacca agtacgatga gaacgataag ttgatcaggg aagtgaaggt
     4261 tatcaccctc aagtcaaagc tcgtgtctga tttcagaaag gatttccaat tctacaaggt
     4321 gagggaaatc aacaactacc accacgctca cgatgcttac cttaacgctg ttgttggaac
     4381 cgctctcatc aagaagtatc ctaagctcga gtcagagttc gtgtacggtg attacaaggt
     4441 gtacgatgtg aggaagatga tcgctaagtc tgagcaagag atcggaaagg ctaccgctaa
     4501 gtatttcttc tactctaaca tcatgaattt cttcaagacc gagattaccc tcgctaacgg
     4561 tgagatcaga aagaggccac tcatcgagac aaacggtgaa acaggtgaga tcgtgtggga
     4621 taagggaagg gatttcgcta ccgttagaaa ggtgctctct atgccacagg tgaacatcgt
     4681 taagaaaacc gaggtgcaga ccggtggatt ctctaaagag tctatcctcc ctaagaggaa
     4741 ctctgataag ctcattgcta ggaagaagga ttgggaccct aagaaatacg gtggtttcga
     4801 ttctcctacc gtggcttact ctgttctcgt tgtggctaag gttgagaagg gaaagagtaa
     4861 gaagctcaag tctgttaagg aacttctcgg aatcactatc atggaaaggt catctttcga
     4921 gaagaaccca atcgatttcc tcgaggctaa gggatacaaa gaggttaaga aggatctcat
     4981 catcaagctc ccaaagtact cactcttcga actcgagaac ggtagaaaga ggatgctcgc
     5041 ttctgctggt gagcttcaaa agggaaacga gcttgctctc ccatctaagt acgttaactt
     5101 tctttacctc gcttctcact acgagaagtt gaagggatct ccagaagata acgagcagaa
     5161 gcaacttttc gttgagcagc acaagcacta cttggatgag atcatcgagc agatctctga
     5221 gttctctaaa agggtgatcc tcgctgatgc aaacctcgat aaggtgttgt ctgcttacaa
     5281 caagcacaga gataagccta tcagggaaca ggcagagaac atcatccatc tcttcaccct
     5341 taccaacctc ggtgctcctg ctgctttcaa gtacttcgat acaaccatcg ataggaagag
     5401 atacacctct accaaagaag tgctcgatgc taccctcatc catcagtcta tcactggact
     5461 ctacgagact aggatcgatc tctcacagct cggtggtgat ggtgggggca gtcctaaaaa
     5521 gaagagaaag gtgggttcgc ctaagaaaaa gaggaaagtt ggtggaggtg gatcaggagg
     5581 tggaggatct ggtgggggag gatccgaagg tgctggatca acttctcacg tgtcatcatc
     5641 acatagggga tttgacctta atattcctcc aatccctgaa ttttctatgg ttaacggaga
     5701 cgacgaggtt atgtctccaa tgcctgctaa aaaacctaga ttcgattttc cagttaaact
     5761 tcagcttgga ggtggtggtt cagagggagc aggatcaact tcacatgtgt catcatctca
     5821 cagaggtttt gaccttaaca ttccacctat ccctgagttt tcaatggtga atggtgatga
     5881 cgaagtgatg tcacctatgc cagctaaaaa gccaagattc gatttccctg tgaagttgca
     5941 attgtaagct tgctctcaag atcaaaggct taaaaagctg gggttttatg aatgggatca
     6001 aagtttcttt ttttctttta tatttgcttc tccatttgtt tgtttcattt ccctttttgt
     6061 tttcgtttct atgatgcact tgtgtgtgac aaactctctg ggtttttact tacgtctgcg
     6121 tttcaaaaaa aaaaaccgct ttcgttttgc gttttagtcc cattgttttg tagctctgag
     6181 tgatcgaatt gatgcctctt tattcctttt gttccctata atttctttca aaactcagaa
     6241 gaaaaacctt gaaactcttt gcaatgttaa tataagtatt gtataagatt tttattgatt
     6301 tggttattag tcttactttt gctacctcca tcttcacttg gaactgatat tctgaatagt
     6361 taaagcgtta catgtgttcc attcacaaat gaacttaaac tagcacaaag tcagatattt
     6421 taagatcgca ccatttcgct acagtgaaga gcaattctga caggatatat tggcgggtaa
     6481 acctaagaga aaagagcgtt tattagaata atcggatatt taaaagggcg tgaaaaggtt
     6541 tatccgttcg tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta
     6601 ctttgatcca acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt
     6661 catctgaaaa cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct
     6721 tttcctggcg ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga
     6781 accggagaca ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg
     6841 tcagcaccga cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca
     6901 ccaagctgtt ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga
     6961 tgcttgacca cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc
     7021 gcagcacccg cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc
     7081 gtagcctggc agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg
     7141 tgttcgccgg cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc
     7201 gcgaggccgc caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac
     7261 agatcgcgca cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg
     7321 cactgcttgg cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga
     7381 cgcccaccga ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg
     7441 ccctggcggc cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg
     7501 ccaggacgaa ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta
     7561 cgtgttcgag ccgcccgcgc acctctcaac cgtgcggctg catgaaatcc tggccggttt
     7621 gtctgatgcc aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg
     7681 ccgtctaaaa aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta
     7741 tatgatgcga tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta
     7801 cttaaccaga aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg
     7861 caactcgccg gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat
     7921 tgggcggccg tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt
     7981 gaccgcgacg tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag
     8041 gcggcggact tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag
     8101 ccaagccctt acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt
     8161 gaggtcacgg atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg
     8221 cgcatcggcg gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc
     8281 cgtatcacgc agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa
     8341 tcagaacccg agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca
     8401 aaactcattt gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg
     8461 ccggccgtcc gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc
     8521 cagccatgaa gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt
     8581 acgcggtacg ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac
     8641 cagagtaaat gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc
     8701 atggaaaatc aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg
     8761 ggcggttggc caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc
     8821 cccaagcccg aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg
     8881 cggcgctggg tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac
     8941 gcatcgaggc agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca
     9001 aagaatcccg gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg
     9061 acgagcaacc agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca
     9121 gcatcatgga cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga
     9181 tccgctacga gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca
     9241 gtgtgtggga ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc
     9301 gataccggga agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg
     9361 tactcaagtt ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct
     9421 gcattcggtt aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc
     9481 gcctggtgac ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg
     9541 aaaccgggcg gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca
     9601 cagaaggcaa gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg
     9661 gcatcggccg ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat
     9721 ggttgttcaa gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt
     9781 tcaccgtgcg caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg
     9841 cggggcaggc tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat
     9901 ccgccggttc ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag
     9961 gtcgaaaagg actctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg
    10021 ggaaccggaa cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt
    10081 aagtgactga tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta
    10141 ttaaaactct taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagccgaag
    10201 tgctgcaaaa agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc
    10261 ggcctatcgc ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag
    10321 ggcgcggaca agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc
    10381 ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc
    10441 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
    10501 gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
    10561 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
    10621 taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcatccgct tcctcgctca
    10681 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
    10741 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
    10801 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
    10861 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
    10921 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
    10981 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
    11041 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
    11101 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
    11161 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
    11221 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
    11281 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
    11341 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
    11401 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    11461 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtgat
    11521 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
    11581 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
    11641 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
    11701 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
    11761 gaatccggtg agaatggcaa aagtttatgc atttctttcc agacttgttc aacaggccag
    11821 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
    11881 gcctgagcga gtcgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
    11941 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
    12001 tcttctaata cctggaatgc tgttttccct gggatcgcag tggtgagtaa ccatgcatca
    12061 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
    12121 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
    12181 aactctggcg catcgggctt cccatacaat cggtagattg tcgcacctga ttgcccgaca
    12241 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
    12301 cttgagcaag acgtttcccg ttgaatatgg ctcat
//
