LOCUS       sequence-486465-        5945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  RAS expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5945)
  AUTHORS   Faraco CCF, Zhu W, Fortier AM, Ramdzan ZM, Vickridge E, Ong V, 
            Goudreau F, Djerir B, Bellemare J, Gu S, Zhang H, Carroll BL, 
            Krishnan SS, Leduy L, Guarne A, Park M, Marechal A, Nepveu A
  TITLE     The function of MYC in base excision repair protects against 
            RAS-induced senescence.
  JOURNAL   Nucleic Acids Res. 2025 Jul 19;53(14):gkaf706. doi: 
            10.1093/nar/gkaf706.
  PUBMED    40737085
REFERENCE   2  (bases 1 to 5945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-07-19"; volume: "53(14):gkaf706. doi"; 
            pages: " 10.1093/nar/gkaf706"
COMMENT     Sequence Label: pBabe-Hygro-RAS
FEATURES             Location/Qualifiers
     source          1..5945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(46..78,79..81,82..615)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     primer_bind     complement(775..795)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        780..1109
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      960..1095
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             1183..2208
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             1183..2208
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     primer_bind     2725..2744
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             3006..3475
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     CDS             complement(join(3590..4381,4382..4450))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     4213..4232
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4451..4555)
                     /gene="bla"
                     /label=AmpR promoter
     LTR             4586..5055
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    5120..5319
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             5520..5936
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     5866..5888
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     5904..5920
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
ORIGIN
        1 ccagtgtggt ggtacagctt ggggggccag ggccggggcc gagcgatgac ggaatataag
       61 ctggtggtgg tgggcgccgt cggtgtgggc aagagtgcgc tgaccatcca gctgatccag
      121 aaccattttg tggacgaata cgaccccact atagaggatt cctaccggaa gcaggtggtc
      181 attgatgggg agacgtgcct gttggacatc ctggataccg ccggccagga ggagtacagc
      241 gccatgcggg accagtacat gcgcaccggg gagggcttcc tgtgtgtgtt tgccatcaac
      301 aacaccaagt cttttgagga catccaccag tacagggagc agatcaaacg ggtgaaggac
      361 tcggatgacg tgcccatggt gctggtgggg aacaagtgtg acctggctgc acgcactgtg
      421 gaatctcggc aggctcagga cctcgcccga agctacggca tcccctacat cgagacctcg
      481 gccaagaccc ggcagggagt ggaggatgcc ttctacacgt tggtgcgtga gatccggcag
      541 cacaagctgc ggaagctgaa ccctcctgat gagagtggcc ccggctgcat gagctgcaag
      601 tgtgtgctct cctgacgcag cacaagctca ggacatggag gtgccggatg caggaaggag
      661 gtgcagacgg aaggaggagg aaggaaggac ggaagcaagg aaggaaggaa gggctgctgg
      721 agcccagtca ccccccaagc tgtaggaatt cgccagcaca gtggtcgacc ctgtggaatg
      781 tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
      841 tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa
      901 gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta actccgccca
      961 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     1021 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     1081 gcttttttgg aggcctaggc ttttgcaaaa agcttgccag ctggggcgcc ctctggtaag
     1141 gttgggaagc cctgcagatc tgatcccggg gggcaatgag atatgaaaaa gcctgaactc
     1201 accgcgacgt ctgtcgagaa gtttctgatc gaaaagttcg acagcgtctc cgacctgatg
     1261 cagctctcgg agggcgaaga atctcgtgct ttcagcttcg atgtaggagg gcgtggatat
     1321 gtcctgcggg taaatagctg cgccgatggt ttctacaaag atcgttatgt ttatcggcac
     1381 tttgcatcgg ccgcgctccc gattccggaa gtgcttgaca ttggggaatt tagcgagagc
     1441 ctgacctatt gcatctcccg ccgtgcacag ggtgtcacgt tgcaagacct gcctgaaacc
     1501 gaactgcccg ctgttctgca gccggtcgcg gaggccatgg atgcgatcgc tgcggccgat
     1561 cttagccaga cgagcgggtt cggcccattc ggaccgcaag gaatcggtca atacactaca
     1621 tggcgtgatt tcatatgcgc gattgctgat ccccatgtgt atcactggca aactgtgatg
     1681 gacgacaccg tcagtgcgtc cgtcgcgcag gctctcgatg agctgatgct ttgggccgag
     1741 gactgccccg aagtccggca cctcgtgcac gcggatttcg gctccaacaa tgtcctgacg
     1801 gacaatggcc gcataacagc ggtcattgac tggagcgagg cgatgttcgg ggattcccaa
     1861 tacgaggtcg ccaacatctt cttctggagg ccgtggttgg cttgtatgga gcagcagacg
     1921 cgctacttcg agcggaggca tccggagctt gcaggatcgc cgcggctccg ggcgtatatg
     1981 ctccgcattg gtcttgacca actctatcag agcttggttg acggcaattt cgatgatgca
     2041 gcttgggcgc agggtcgatg cgacgcaatc gtccgatccg gagccgggac tgtcgggcgt
     2101 acacaaatcg cccgcagaag cgcggccgtc tggaccgatg gctgtgtaga agtactcgcc
     2161 gatagtggaa accgacgccc cagcactcgt ccgagggcaa aggaatagag tagatgccga
     2221 ccgggatcta tcgatgcggc gggtgtggtg gttacgcgta atctgctgct tgcaaacaaa
     2281 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     2341 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     2401 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     2461 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg
     2521 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag
     2581 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc
     2641 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg
     2701 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt
     2761 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg
     2821 gaaaaacgcc agcaacgcat cgataaaata aaagatttta tttagtctcc agaaaaaggg
     2881 gggaatgaaa gaccccacct gtaggtttgg caagctagct taagtaacgc cattttgcaa
     2941 ggcatggaaa aatacataac tgagaataga gaagttcaga tcaaggtcag gaacagatgg
     3001 aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
     3061 gccaagaaca gatggaacag ctgaatatgg gccaaacagg atatctgtgg taagcagttc
     3121 ctgccccggc tcagggccaa gaacagatgg tccccagatg cggtccagcc ctcagcagtt
     3181 tctagagaac catcagatgt ttccagggtg ccccaaggac ctgaaatgac cctgtgcctt
     3241 atttgaacta accaatcagt tcgcttctcg cttctgttcg cgcgcttctg ctccccgagc
     3301 tcaataaaag agcccacaac ccctcactcg gggcgccagt cctccgattg actgagtcgc
     3361 ccgggtaccc gtgtatccaa taaaccctct tgcagttgca tccgacttgt ggtctcgctg
     3421 ttccttggga gggtctcctc tgagtgattg actacccgtc agcgggggtc tttcacatgc
     3481 agcatgtatc aaaattaatt tggttttttt tcttaagtat ttacattaaa tggccatagt
     3541 gcggccgcaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     3601 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     3661 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     3721 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     3781 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     3841 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     3901 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     3961 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     4021 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     4081 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     4141 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     4201 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     4261 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     4321 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     4381 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     4441 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     4501 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     4561 ttccccgaaa agtgccacct gcagcctgaa tatgggccaa acaggatatc tgtggtaagc
     4621 agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg gccaaacagg
     4681 atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg tccccagatg
     4741 cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg ccccaaggac
     4801 ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg cttctgttcg
     4861 cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg gggcgccagt
     4921 cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct tgcagttgca
     4981 tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg actacccgtc
     5041 agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc cagggaccac
     5101 cgacccacca ccgggaggca agctggccag caacttatct gtgtctgtcc gattgtctag
     5161 tgtctatgac tgattttatg cgcctgcgtc ggtactagtt agctaactag ctctgtatct
     5221 ggcggacccg tggtggaact gacgagttct gaacacccgg ccgcaaccct gggagacgtc
     5281 ccagggactt tgggggccgt ttttgtggcc cgacctgagg aagggagtcg atgtggaatc
     5341 cgaccccgtc aggatatgtg gttctggtag gagacgagaa cctaaaacag ttcccgcctc
     5401 cgtctgaatt tttgctttcg gtttggaacc gaagccgcgc gtcttgtctg ctgcagcgct
     5461 gcagcatcgt tctgtgttgt ctctgtctga ctgtgtttct gtatttgtct gaaaattagg
     5521 gccagactgt taccactccc ttaagtttga ccttaggtca ctggaaagat gtcgagcgga
     5581 tcgctcacaa ccagtcggta gatgtcaaga agagacgttg ggttaccttc tgctctgcag
     5641 aatggccaac ctttaacgtc ggatggccgc gagacggcac ctttaaccga gacctcatca
     5701 cccaggttaa gatcaaggtc ttttcacctg gcccgcatgg acacccagac caggtcccct
     5761 acatcgtgac ctgggaagcc ttggcttttg acccccctcc ctgggtcaag ccctttgtac
     5821 accctaagcc tccgcctcct cttcctccat ccgccccgtc tctccccctt gaacctcctc
     5881 gttcgacccc gcctcgatcc tccctttatc cagccctcac tccttctcta ggcgccggcc
     5941 ggatc
//
