LOCUS       sequence-486585-       20458 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  for chromosome editing.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 20458)
  AUTHORS   Sun C, Li H, Liu Y, Li Y, Gao R, Shi X, Fei H, Liu J, Liang R, Gao C
  TITLE     Iterative recombinase technologies for efficient and precise genome 
            engineering across kilobase to megabase scales.
  JOURNAL   Cell. 2025 Aug 1:S0092-8674(25)00800-1. doi: 
            10.1016/j.cell.2025.07.011.
  PUBMED    40763736
REFERENCE   2  (bases 1 to 20458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2025 
            Aug 1:S0092-8674(25)00800-1. doi: 10.1016/j.cell.2025.07.011."
COMMENT     Sequence Label: pH-PCE
FEATURES             Location/Qualifiers
     source          1..20458
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     233..255
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     247..264
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     248..264
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     ncRNA           1414..1481
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     terminator      1512..1760
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     promoter        1783..3775
                     /label=Ubi promoter
                     /note="maize polyubiquitin gene promoter"
     CDS             3786..3812
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             3852..3872
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     RBS             7886..7894
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             8196..8216
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             9942..9962
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             11088..11114
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             11154..11174
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(11833..11849)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11833..11849)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11846..11868)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11857..11873
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11881..11887,11888..11905,11906..11911))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11926..11947
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12138..12814
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     12751..12771
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             12881..13906
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     K"
     polyA_signal    13947..14121
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    14199..14223
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             14648..15442
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      15529..16117
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16018..16037
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     16271..16288
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    16303..16443
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(16369..16388)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     16529..16551
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      16787..16981
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(17047..18120)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(18549..19178)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagttta
       61 aactgaaggc gggaaacgac aatctgatcc aagctcaagc tgctctagca ttcgccattc
      121 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgccagctg
      181 gcgaaagggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt ttcccagtca
      241 cgacgttgta aaacgacggc cagtgaattc atggagtcaa agattcaaat agaggaccta
      301 acagaactcg ccgtaaagac tggcgaacag ttcatacaga gtctcttacg actcaatgac
      361 aagaagaaaa tcttcgtcaa catggtggag cacgacacac ttgtctactc caaaaatatc
      421 aaagatacag tctcagaaga ccaaagggca attgagactt ttcaacaaag ggtaatatcc
      481 ggaaacctcc tcggattcca ttgcccagct atctgtcact ttattgtgaa gatagtggaa
      541 aaggaaggtg gctcctacaa atgccatcat tgcgataaag gaaaggccat cgttgaagat
      601 gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat cgtggaaaaa
      661 gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgattggca gacatactgt
      721 cccacaaatg aagatggaat ctgtaaaaga aaacgcgtga aataatgcgt ctgacaaagg
      781 ttaggtcggc tgcctttaat caataccaaa gtggtcccta ccacgatgga aaaactgtgc
      841 agtcggtttg gctttttctg acgaacaaat aagattcgtg gccgacaggt gggggtccac
      901 catgtgaagg catcttcaga ctccaataat ggagcaatga cgtaagggct tacgaaataa
      961 gtaagggtag tttgggaaat gtccactcac ccgtcagtct ataaatactt agcccctccc
     1021 tcattgttaa gggagcaaaa tctcagagag atagtcctag agagagaaag agagcaagta
     1081 gcctagaagt agtcaaggcg gcgaagtatt caggcacgtg gccaggaaga agaaaagcca
     1141 agacgacgaa aacaggtaag agctaagcat ctagataagt tgaaaacaat cttcaaaagt
     1201 cccacatcgc ttagataaga aaacgaagct gagtttatat acagctagag tcgaagtagt
     1261 gattgaacaa agcaccagtg gtctagtggt agaatagtac cctgccacgg tacagacccg
     1321 ggttcgattc ccggctggtg catgagacct tcggggaata tgagcgtcag gtctcacgcg
     1381 gttctatcta gttacgcgtt aaaccaacta gaaggccggc atggtcccag cctcctcgct
     1441 ggcgccggct gggcaacatg cttcggcatg gcgaatggga cttttttttg atatctccgg
     1501 ggctaattga atatgaagat gaagatgaaa tatttggtgt gtcaaataaa aagctggtgt
     1561 gcttaagttt gtgttttttt cttggcttgt tgtgttatga atttgtggct ttttctaata
     1621 ttaaatgaat gtaagatctc attataatga ataaacaaat gtttctataa tccattgtga
     1681 atgttttgtt ggatctcttc tgcagcatat aactactgta tgtgctatgg tatggactat
     1741 ggaatatgat taaagataag aagcttggcg taatctgcat gcctgcagtg cagcgtgacc
     1801 cggtcgtgcc cctctctaga gataatgagc attgcatgtc taagttataa aaaattacca
     1861 catatttttt ttgtcacact tgtttgaagt gcagtttatc tatctttata catatattta
     1921 aactttactc tacgaataat ataatctata gtactacaat aatatcagtg ttttagagaa
     1981 tcatataaat gaacagttag acatggtcta aaggacaatt gagtattttg acaacaggac
     2041 tctacagttt tatcttttta gtgtgcatgt gttctccttt ttttttgcaa atagcttcac
     2101 ctatataata cttcatccat tttattagta catccattta gggtttaggg ttaatggttt
     2161 ttatagacta atttttttag tacatctatt ttattctatt ttagcctcta aattaagaaa
     2221 actaaaactc tattttagtt tttttattta ataatttaga tataaaatag aataaaataa
     2281 agtgactaaa aattaaacaa atacccttta agaaattaaa aaaactaagg aaacattttt
     2341 cttgtttcga gtagataatg ccagcctgtt aaacgccgtc gacgagtcta acggacacca
     2401 accagcgaac cagcagcgtc gcgtcgggcc aagcgaagca gacggcacgg catctctgtc
     2461 gctgcctctg gacccctctc gagagttccg ctccaccgtt ggacttgctc cgctgtcggc
     2521 atccagaaat tgcgtggcgg agcggcagac gtgagccggc acggcaggcg gcctcctcct
     2581 cctctcacgg caccggcagc tacgggggat tcctttccca ccgctccttc gctttccctt
     2641 cctcgcccgc cgtaataaat agacaccccc tccacaccct ctttccccaa cctcgtgttg
     2701 ttcggagcgc acacacacac aaccagatct cccccaaatc cacccgtcgg cacctccgct
     2761 tcaaggtacg ccgctcgtcc tccccccccc cccctctcta ccttctctag atcggcgttc
     2821 cggtccatgg ttagggcccg gtagttctac ttctgttcat gtttgtgtta gatccgtgtt
     2881 tgtgttagat ccgtgctgct agcgttcgta cacggatgcg acctgtacgt cagacacgtt
     2941 ctgattgcta acttgccagt gtttctcttt ggggaatcct gggatggctc tagccgttcc
     3001 gcagacggga tcgatttcat gatttttttt gtttcgttgc atagggtttg gtttgccctt
     3061 ttcctttatt tcaatatatg ccgtgcactt gtttgtcggg tcatcttttc atgctttttt
     3121 ttgtcttggt tgtgatgatg tggtctggtt gggcggtcgt tctagatcgg agtagaattc
     3181 tgtttcaaac tacctggtgg atttattaat tttggatctg tatgtgtgtg ccatacatat
     3241 tcatagttac gaattgaaga tgatggatgg aaatatcgat ctaggatagg tatacatgtt
     3301 gatgcgggtt ttactgatgc atatacagag atgctttttg ttcgcttggt tgtgatgatg
     3361 tggtgtggtt gggcggtcgt tcattcgttc tagatcggag tagaatactg tttcaaacta
     3421 cctggtgtat ttattaattt tggaactgta tgtgtgtgtc atacatcttc atagttacga
     3481 gtttaagatg gatggaaata tcgatctagg ataggtatac atgttgatgt gggttttact
     3541 gatgcatata catgatggca tatgcagcat ctattcatat gctctaacct tgagtaccta
     3601 tctattataa taaacaagta tgttttataa ttattttgat cttgatatac ttggatgatg
     3661 gcatatgcag cagctatatg tggatttttt tagccctgcc ttcatacgct atttatttgc
     3721 ttggtactgt ttcttttgtc gatgctcacc ctgttgtttg gtgttacttc tgcagcccta
     3781 ggatgcctgc cgccaagaga gtcaagctcg atggcggcaa gaggaccgcc gatggcagcg
     3841 agttcgagtc tcctaagaag aagaggaagg tcgacaagaa gtactcgatc ggcctcgata
     3901 ttgggactaa ctctgttggc tgggccgtga tcaccgacga gtacaaggtg ccctcaaaga
     3961 agttcaaggt cctgggcaac accgatcggc attccatcaa gaagaatctc attggcgctc
     4021 tcctgttcga cagcggcgag acggctgagg ctacgcggct caagcgcacc gcccgcaggc
     4081 ggtacacgcg caggaagaat cgcatctgct acctgcagga gattttctcc aacgagatgg
     4141 cgaaggttga cgattctttc ttccacaggc tggaggagtc attcctcgtg gaggaggata
     4201 agaagcacga gcggcatcca atcttcggca acattgtcga cgaggttgcc taccacgaga
     4261 agtaccctac gatctaccat ctgcggaaga agctcgtgga ctccacagat aaggcggacc
     4321 tccgcctgat ctacctcgct ctggcccaca tgattaagtt caggggccat ttcctgatcg
     4381 agggggatct caacccggac aatagcgatg ttgacaagct gttcatccag ctcgtgcaga
     4441 cgtacaacca gctcttcgag gagaacccca ttaatgcgtc aggcgtcgac gcgaaggcta
     4501 tcctgtccgc taggctctcg aagtctcgga agctcgagaa cctgatcgcc cagctgccgg
     4561 gcgagaagaa gaacggcctg ttcgggaatc tcattgcgct cagcctgggg ctcacgccca
     4621 acttcaagtc gaatttcgat ctcgctgagg acgccaagct gcagctctcc aaggacacat
     4681 acgacgatga cctggataac ctcctggccc agatcggcga tcagtacgcg gacctgttcc
     4741 tcgctgccaa gaatctgtcg gacgccatcc tcctgtctga tattctcagg gtgaacaccg
     4801 agattacgaa ggctccgctc tcagcctcca tgatcaagcg ctacgacgag caccatcagg
     4861 atctgaccct cctgaaggcg ctggtcaggc agcagctccc cgagaagtac aaggagatct
     4921 tcttcgatca gtcgaagaac ggctacgctg ggtacattga cggcggggcc tctcaggagg
     4981 agttctacaa gttcatcaag ccgattctgg agaagatgga cggcacggag gagctgctgg
     5041 tgaagctcaa gcgcgaggac ctcctgagga agcagcggac attcgataac ggcagcatcc
     5101 cacaccagat tcatctcggg gagctgcacg ctatcctgag gaggcaggag gacttctacc
     5161 ctttcctcaa ggataaccgc gagaagatcg agaagattct gactttcagg atcccgtact
     5221 acgtcggccc actcgctagg ggcaactccc gcttcgcttg gatgacccgc aagtcagagg
     5281 agacgatcac gccgtggaac ttcgaggagg tggtcgacaa gggcgctagc gctcagtcgt
     5341 tcatcgagag gatgacgaat ttcgacaaga acctgccaaa tgagaaggtg ctccctaagc
     5401 actcgctcct gtacgagtac ttcacagtct acaacgagct gactaaggtg aagtatgtga
     5461 ccgagggcat gaggaagccg gctttcctgt ctggggagca gaagaaggcc atcgtggacc
     5521 tcctgttcaa gaccaaccgg aaggtcacgg ttaagcagct caaggaggac tacttcaaga
     5581 agattgagtg cttcgattcg gtcgagatct ctggcgttga ggaccgcttc aacgcctccc
     5641 tggggaccta ccacgatctc ctgaagatca ttaaggataa ggacttcctg gacaacgagg
     5701 agaatgagga tatcctcgag gacattgtgc tgacactcac tctgttcgag gaccgggaga
     5761 tgatcgagga gcgcctgaag acttacgccc atctcttcga tgacaaggtc atgaagcagc
     5821 tcaagaggag gaggtacacc ggctggggga ggctgagcag gaagctcatc aacggcattc
     5881 gggacaagca gtccgggaag acgatcctcg acttcctgaa gagcgatggc ttcgcgaacc
     5941 gcaatttcat gcagctgatt cacgatgaca gcctcacatt caaggaggat atccagaagg
     6001 ctcaggtgag cggccagggg gactcgctgc acgagcatat cgcgaacctc gctggctcgc
     6061 cagctatcaa gaaggggatt ctgcagaccg tgaaggttgt ggacgagctg gtgaaggtca
     6121 tgggcaggca caagcctgag aacatcgtca ttgagatggc ccgggagaat cagaccacgc
     6181 agaagggcca gaagaactca cgcgagagga tgaagaggat cgaggagggc attaaggagc
     6241 tggggtccca gatcctcaag gagcacccgg tggagaacac gcagctgcag aatgagaagc
     6301 tctacctgta ctacctccag aatggccgcg atatgtatgt ggaccaggag ctggatatta
     6361 acaggctcag cgattacgac gtcgatgcca tcgttccaca gtcattcctg aaggatgact
     6421 ccattgacaa caaggtcctc accaggtcgg acaagaaccg gggcaagtct gataatgttc
     6481 cttcagagga ggtcgttaag aagatgaaga actactggcg ccagctcctg aatgccaagc
     6541 tgatcacgca gcggaagttc gataacctca caaaggctga gaggggcggg ctctctgagc
     6601 tggacaaggc gggcttcatc aagaggcagc tggtcgagac acggcagatc actaagcacg
     6661 ttgcgcagat tctcgactca cggatgaaca ctaagtacga tgagaatgac aagctgatcc
     6721 gcgaggtgaa ggtcatcacc ctgaagtcaa agctcgtctc cgacttcagg aaggatttcc
     6781 agttctacaa ggttcgggag atcaacaatt accaccatgc ccatgacgcg tacctgaacg
     6841 cggtggtcgg cacagctctg atcaagaagt acccaaagct cgagagcgag ttcgtgtacg
     6901 gggactacaa ggtttacgat gtgaggaaga tgatcgccaa gtcggagcag gagattggca
     6961 aggctaccgc caagtacttc ttctactcta acattatgaa tttcttcaag acagagatca
     7021 ctctggccaa tggcgagatc cggaagcgcc ccctcatcga gacgaacggc gagacggggg
     7081 agatcgtgtg ggacaagggc agggatttcg cgaccgtcag gaaggttctc tccatgccac
     7141 aagtgaatat cgtcaagaag acagaggtcc agactggcgg gttctctaag gagtcaattc
     7201 tgcctaagcg gaacagcgac aagctcatcg cccgcaagaa ggactgggat ccgaagaagt
     7261 acggcgggtt cgacagcccc actgtggcct actcggtcct ggttgtggcg aaggttgaga
     7321 agggcaagtc caagaagctc aagagcgtga aggagctgct ggggatcacg attatggagc
     7381 gctccagctt cgagaagaac ccgatcgatt tcctggaggc gaagggctac aaggaggtga
     7441 agaaggacct gatcattaag ctccccaagt actcactctt cgagctggag aacggcagga
     7501 agcggatgct ggcttccgct ggcgagctgc agaaggggaa cgagctggct ctgccgtcca
     7561 agtatgtgaa cttcctctac ctggcctccc actacgagaa gctcaagggc agccccgagg
     7621 acaacgagca gaagcagctg ttcgtcgagc agcacaagca ttacctcgac gagatcattg
     7681 agcagatttc cgagttctcc aagcgcgtga tcctggccga cgcgaatctg gataaggtcc
     7741 tctccgcgta caacaagcac cgcgacaagc caatcaggga gcaggctgag aatatcattc
     7801 atctcttcac cctgacgaac ctcggcgccc ctgctgcttt caagtacttc gacacaacta
     7861 tcgatcgcaa gaggtacaca agcactaagg aggtcctgga cgcgaccctc atccaccagt
     7921 cgattaccgg cctctacgag acgcgcatcg acctgtctca gctcgggggc gacgaattct
     7981 ccgggagcga gacgccaggc acctccgagt cggccacccc agaatctgcc acagtggtgt
     8041 ccggccaaaa gcaggaccgc cagggcggag aacgcagaag gtcccagctc gatagggatc
     8101 agtgtgccta ctgcaaggag aagggccact gggccaaaga ctgcccgaaa aagccgcgcg
     8161 gcccacgcgg cccaaggcca caaacatccc tccttccaaa gaagaagcgg aaggtggagc
     8221 tcagcggagg atcttccgga ggatctagcg gctccgagac accaggaaca tccgaaagcg
     8281 ctacaccaga atctagcgga ggctcttccg gaggatctag gcctaccctc aacatcgagg
     8341 atgagtatcg cctccacgaa acctccaaag aaccggacgt gtccctcggc agcacatggc
     8401 tcagcgactt cccacaagcg tgggccgaaa ccggcggcat gggcctcgcc gtccgccaag
     8461 ccccactcat tatcccgctg aaggcgacct ccacaccggt gtccatcaag cagtacccga
     8521 tgagccaaga ggcgaggctc gggattaagc cgcacattca gcgcctcctc gatcaaggca
     8581 ttctcgtgcc gtgccaatcc ccgtggaata caccactcct cccggtcaaa aagccgggca
     8641 ccaacgacta tcgcccggtc caagatctcc gcgaggtcaa caagcgcgtg gaagacatcc
     8701 acccgaccgt cccgaacccg tataatctgc tctccgggct cccaccatcc caccagtggt
     8761 atacagtgct ggacctcaaa gacgccttct tctgtctccg cctccaccca acaagccagc
     8821 cgctcttcgc cttcgagtgg cgcgacccgg agatgggcat ctccggccaa ctgacatgga
     8881 cacgcctccc gcaaggcttc aagaacagcc cgacactctt caacgaggcg ctccataggg
     8941 acctcgcgga ttttcgcatc cagcatccgg acctcatcct cctccagtat gcggatgatc
     9001 tcctcctcgc cgcgacctcc gagctggatt gtcaacaagg cacacgcgcg ctcctccaaa
     9061 cactcgggaa cctcggctat cgcgcgtccg cgaaaaaggc ccaaatctgc cagaagcaag
     9121 tgaagtacct cgggtatctg ctcaaggaag gccaacgctg gctcaccgaa gcgcgcaaag
     9181 aaacagtgat ggggcaaccg acaccgaaaa caccacgcca gctgcgcgag tttctcggca
     9241 aagccggctt ctgtcgcctc ttcatcccgg gctttgccga gatggccgcg ccactctacc
     9301 cactcaccaa gccgggcaca ctgtttaact gggggccgga tcagcagaaa gcctaccaag
     9361 agatcaaaca agcgctcctc accgccccag cgctcgggct cccagatctc acaaagccgt
     9421 tcgagctgtt cgtcgatgag aagcaaggct acgcgaaggg cgtgctcaca cagaagctcg
     9481 gcccgtggag gaggccagtg gcctatctct ccaaaaaact cgatccagtg gccgccggct
     9541 ggccaccgtg tctgcgcatg gtcgccgcga ttgccgtgct cacaaaggat gccggcaaac
     9601 tcacaatggg ccagccgctg gtgatcctcg cgccacatgc cgtggaagcc ctcgtcaaac
     9661 agccgccgga taggtggctc tccaatgcgc gcatgaccca ttaccaagcg ctcctcctcg
     9721 acaccgatcg cgtccagttc ggcccagtgg tcgccctcaa tccggcgaca ctgctgccac
     9781 tcccagagga gggcctccaa cacaactgtc tggatattct cgcggaagcg catggcacaa
     9841 ggccagacct cacagatcaa ccgctcagcg gcggcagcaa gaggaccgcc gactcccagc
     9901 actcgacccc gccgaagacc aagaggaagg tggagttcga gccgaagaag aagaggaagg
     9961 tctccggcgg atcttccgga ggatctagcg gctccgagac accaggaaca tccgaatccg
    10021 ctacaccaga gtcttctgga ggatctagcg gaggatctat gagtaatctc ttgaccgttc
    10081 atcaaaattt accggcgctc ccggtggacg ccacaagcga tgaggtgcgg aagaacctga
    10141 tggatatgtt ccgtgatcgg caggcgttta gcgaagagac atggaagatg cttctttccg
    10201 tgtgtcgtag ttgggcagcg tggtgcaagc tcaacaaccg gaagtggttc cctgctgagc
    10261 cggaagacgt cagggactat ttactctacc tccaagctag ggggttagca gttaaaacta
    10321 tacagcagca tctcggtcaa ttgaacatgc tgcaccggcg cagcggactg ccgcggccca
    10381 gtgactctaa tgctgtgtcg ctcgtgatga ggcgcatcag aaaggagaat gtggatgctg
    10441 gtgaacgagc caagcaggcg cttgccttcg agaggacaca gtttgatcaa gttcgcagcc
    10501 tcatggagaa cagcgatcgt tgccaagaca tcaggaatct agccttcctg ggtatcgcct
    10561 acaatactct gttgagaatt gcggagattg cgaggatccg tgtgaaggac ataagccgaa
    10621 ctgatggtgg gaggatgttg attcacattg gacgcaccaa gacactcgta tcaaccgcag
    10681 gagtcgagaa ggctctatcg cttggggtta ctaagttggt cgaacgatgg atatcagtat
    10741 ccggcgtcgc cgacgatcca aacaattacc tgttctgccg cgtgcgcaag aatggggttg
    10801 cggcgccttc agctacgtcc cagctctcga cgagggcact ggagggcatc tttgaggcta
    10861 cgcacaggct gatctatggc ccgccggatg attccggcgg gagatacttg gcatggtctg
    10921 gacactccgc cagagtcggc gccgcccgcg acatggcgcg ggccggcgtg tcaattccag
    10981 aaataatgca ggcaggaggg tggaccaatg tcaacattgt aatgaactat atcagaaacc
    11041 tcgactcgga gacaggagcc atggtcaggc ttctagaaga cggtgaccca gcagctaaac
    11101 gcgtaaagtt ggatggaggt aagaggacag cggatggatc tgaattcgag agcccgaaaa
    11161 agaagaggaa agtctgagag ctcagagctt tcgttcgtat catcggtttc gacaacgttc
    11221 gtcaagttca atgcatcagt ttcattgcgc acacaccaga atcctactga gtttgagtat
    11281 tatggcattg ggaaaactgt ttttcttgta ccatttgttg tgcttgtaat ttactgtgtt
    11341 ttttattcgg ttttcgctat cgaactgtga aatggaaatg gatggagaag agttaatgaa
    11401 tgatatggtc cttttgttca ttctcaaatt aatattattt gttttttctc ttatttgttg
    11461 tgtgttgaat ttgaaattat aagagatatg caaacatttt gttttgagta aaaatgtgtc
    11521 aaatcgtggc ctctaatgac cgaagttaat atgaggagta aaacacttgt agttgtacca
    11581 ttatgcttat tcactaggca acaaatatat tttcagacct agaaaagctg caaatgttac
    11641 tgaatacaag tatgtcctct tgtgttttag acatttatga actttccttt atgtaatttt
    11701 ccagaatcct tgtcagattc taatcattgc tttataatta tagttatact catggatttg
    11761 tagttgagta tgaaaatatt ttttaatgca ttttatgact tgccaattga ttgacaacga
    11821 attcgtaatc atgtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
    11881 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
    11941 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
    12001 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattggct agagcagctt
    12061 gccaacatgg tggagcacga cactctcgtc tactccaaga atatcaaaga tacagtctca
    12121 gaagaccaaa gggctattga gacttttcaa caaagggtaa tatcgggaaa cctcctcgga
    12181 ttccattgcc cagctatctg tcacttcatc aaaaggacag tagaaaagga aggtggcacc
    12241 tacaaatgcc atcattgcga taaaggaaag gctatcgttc aagatgcctc tgccgacagt
    12301 ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga cgttccaacc
    12361 acgtcttcaa agcaagtgga ttgatgtgaa catggtggag cacgacactc tcgtctactc
    12421 caagaatatc aaagatacag tctcagaaga ccaaagggct attgagactt ttcaacaaag
    12481 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcaaaag
    12541 gacagtagaa aaggaaggtg gcacctacaa atgccatcat tgcgataaag gaaaggctat
    12601 cgttcaagat gcctctgccg acagtggtcc caaagatgga cccccaccca cgaggagcat
    12661 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatatctc
    12721 cactgacgta agggatgacg cacaatccca ctatccttcg caagaccctt cctctatata
    12781 aggaagttca tttcatttgg agaggacacg ctgaaatcac cagtctctct ctacaaatct
    12841 atctctctcg agctttcgca gatccggggg gcaatgagat atgaaaaagc ctgaactcac
    12901 cgcgacgtct gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca
    12961 gctctcggag ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt
    13021 cctgcgggta aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt
    13081 tgcatcggcc gcgctcccga ttccggaagt gcttgacatt ggggagttta gcgagagcct
    13141 gacctattgc atctcccgcc gttcacaggg tgtcacgttg caagacctgc ctgaaaccga
    13201 actgcccgct gttctacaac cggtcgcgga ggctatggat gcgatcgctg cggccgatct
    13261 tagccagacg agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg
    13321 gcgtgatttc atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga
    13381 cgacaccgtc agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga
    13441 ctgccccgaa gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga
    13501 caatggccgc ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata
    13561 cgaggtcgcc aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg
    13621 ctacttcgag cggaggcatc cggagcttgc aggatcgcca cgactccggg cgtatatgct
    13681 ccgcattggt cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc
    13741 ttgggcgcag ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac
    13801 acaaatcgcc cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga
    13861 tagtggaaac cgacgcccca gcactcgtcc gagggcaaag aaatagagta gatgccgacc
    13921 gggatctgtc gatcgacaag ctcgagtttc tccataataa tgtgtgagta gttcccagat
    13981 aagggaatta gggttcctat agggtttcgc tcatgtgttg agcatataag aaacccttag
    14041 tatgtatttg tatttgtaaa atacttctat caataaaatt tctaattcct aaaaccaaaa
    14101 tccagtacta aaatccagat cccccgaatt aattcggcgt taattcagta cattaaaaac
    14161 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
    14221 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
    14281 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
    14341 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
    14401 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
    14461 accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
    14521 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
    14581 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
    14641 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
    14701 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
    14761 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
    14821 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
    14881 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
    14941 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
    15001 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
    15061 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
    15121 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
    15181 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
    15241 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
    15301 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
    15361 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
    15421 tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt aacgtgagtt
    15481 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    15541 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    15601 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    15661 gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    15721 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    15781 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    15841 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    15901 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
    15961 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    16021 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    16081 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    16141 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    16201 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    16261 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct
    16321 ccttacgcat ctgtgcggta tttcacaccg catatggtgc actctcagta caatctgctc
    16381 tgatgccgca tagttaagcc agtatacact ccgctatcgc tacgtgactg ggtcatggct
    16441 gcgccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct gctcccggca
    16501 tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag gttttcaccg
    16561 tcatcaccga aacgcgcgag gcagggtgcc ttgatgtggg cgccggcggt cgagtggcga
    16621 cggcgcggct tgtccgcgcc ctggtagatt gcctggccgt aggccagcca tttttgagcg
    16681 gccagcggcc gcgataggcc gacgcgaagc ggcggggcgt agggagcgca gcgaccgaag
    16741 ggtaggcgct ttttgcagct cttcggctgt gcgctggcca gacagttatg cacaggccag
    16801 gcgggtttta agagttttaa taagttttaa agagttttag gcggaaaaat cgcctttttt
    16861 ctcttttata tcagtcactt acatgtgtga ccggttccca atgtacggct ttgggttccc
    16921 aatgtacggg ttccggttcc caatgtacgg ctttgggttc ccaatgtacg tgctatccac
    16981 aggaaacaga ccttttcgac ctttttcccc tgctagggca atttgcccta gcatctgctc
    17041 cgtacattag gaaccggcgg atgcttcgcc ctcgatcagg ttgcggtagc gcatgactag
    17101 gatcgggcca gcctgccccg cctcctcctt caaatcgtac tccggcaggt catttgaccc
    17161 gatcagcttg cgcacggtga aacagaactt cttgaactct ccggcgctgc cactgcgttc
    17221 gtagatcgtc ttgaacaacc atctggcttc tgccttgcct gcggcgcggc gtgccaggcg
    17281 gtagagaaaa cggccgatgc cgggatcgat caaaaagtaa tcggggtgaa ccgtcagcac
    17341 gtccgggttc ttgccttctg tgatctcgcg gtacatccaa tcagctagct cgatctcgat
    17401 gtactccggc cgcccggttt cgctctttac gatcttgtag cggctaatca aggcttcacc
    17461 ctcggatacc gtcaccaggc ggccgttctt ggccttcttc gtacgctgca tggcaacgtg
    17521 cgtggtgttt aaccgaatgc aggtttctac caggtcgtct ttctgctttc cgccatcggc
    17581 tcgccggcag aacttgagta cgtccgcaac gtgtggacgg aacacgcggc cgggcttgtc
    17641 tcccttccct tcccggtatc ggttcatgga ttcggttaga tgggaaaccg ccatcagtac
    17701 caggtcgtaa tcccacacac tggccatgcc ggccggccct gcggaaacct ctacgtgccc
    17761 gtctggaagc tcgtagcgga tcacctcgcc agctcgtcgg tcacgcttcg acagacggaa
    17821 aacggccacg tccatgatgc tgcgactatc gcgggtgccc acgtcataga gcatcggaac
    17881 gaaaaaatct ggttgctcgt cgcccttggg cggcttccta atcgacggcg caccggctgc
    17941 cggcggttgc cgggattctt tgcggattcg atcagcggcc gcttgccacg attcaccggg
    18001 gcgtgcttct gcctcgatgc gttgccgctg ggcggcctgc gcggccttca acttctccac
    18061 caggtcatca cccagcgccg cgccgatttg taccgggccg gatggtttgc gaccgctcac
    18121 gccgattcct cgggcttggg ggttccagtg ccattgcagg gccggcaggc aacccagccg
    18181 cttacgcctg gccaaccgcc cgttcctcca cacatggggc attccacggc gtcggtgcct
    18241 ggttgttctt gattttccat gccgcctcct ttagccgcta aaattcatct actcatttat
    18301 tcatttgctc atttactctg gtagctgcgc gatgtattca gatagcagct cggtaatggt
    18361 cttgccttgg cgtaccgcgt acatcttcag cttggtgtga tcctccgccg gcaactgaaa
    18421 gttgacccgc ttcatggctg gcgtgtctgc caggctggcc aacgttgcag ccttgctgct
    18481 gcgtgcgctc ggacggccgg cacttagcgt gtttgtgctt ttgctcattt tctctttacc
    18541 tcattaactc aaatgagttt tgatttaatt tcagcggcca gcgcctggac ctcgcgggca
    18601 gcgtcgccct cgggttctga ttcaagaacg gttgtgccgg cggcggcagt gcctgggtag
    18661 ctcacgcgct gcgtgatacg ggactcaaga atgggcagct cgtacccggc cagcgcctcg
    18721 gcaacctcac cgccgatgcg cgtgcctttg atcgcccgcg acacgacaaa ggccgcttgt
    18781 agccttccat ccgtgacctc aatgcgctgc ttaaccagct ccaccaggtc ggcggtggcc
    18841 catatgtcgt aagggcttgg ctgcaccgga atcagcacga agtcggctgc cttgatcgcg
    18901 gacacagcca agtccgccgc ctggggcgct ccgtcgatca ctacgaagtc gcgccggccg
    18961 atggccttca cgtcgcggtc aatcgtcggg cggtcgatgc cgacaacggt tagcggttga
    19021 tcttcccgca cggccgccca atcgcgggca ctgccctggg gatcggaatc gactaacaga
    19081 acatcggccc cggcgagttg cagggcgcgg gctagatggg ttgcgatggt cgtcttgcct
    19141 gacccgcctt tctggttaag tacagcgata accttcatgc gttccccttg cgtatttgtt
    19201 tatttactca tcgcatcata tacgcagcga ccgcatgacg caagctgttt tactcaaata
    19261 cacatcacct ttttagacgg cggcgctcgg tttcttcagc ggccaagctg gccggccagg
    19321 ccgccagctt ggcatcagac aaaccggcca ggatttcatg cagccgcacg gttgagacgt
    19381 gcgcgggcgg ctcgaacacg tacccggccg cgatcatctc cgcctcgatc tcttcggtaa
    19441 tgaaaaacgg ttcgtcctgg ccgtcctggt gcggtttcat gcttgttcct cttggcgttc
    19501 attctcggcg gccgccaggg cgtcggcctc ggtcaatgcg tcctcacgga aggcaccgcg
    19561 ccgcctggcc tcggtgggcg tcacttcctc gctgcgctca agtgcgcggt acagggtcga
    19621 gcgatgcacg ccaagcagtg cagccgcctc tttcacggtg cggccttcct ggtcgatcag
    19681 ctcgcgggcg tgcgcgatct gtgccggggt gagggtaggg cgggggccaa acttcacgcc
    19741 tcgggccttg gcggcctcgc gcccgctccg ggtgcggtcg atgattaggg aacgctcgaa
    19801 ctcggcaatg ccggcgaaca cggtcaacac catgcggccg gccggcgtgg tggtgtcggc
    19861 ccacggctct gccaggctac gcaggcccgc gccggcctcc tggatgcgct cggcaatgtc
    19921 cagtaggtcg cgggtgctgc gggccaggcg gtctagcctg gtcactgtca caacgtcgcc
    19981 agggcgtagg tggtcaagca tcctggccag ctccgggcgg tcgcgcctgg tgccggtgat
    20041 cttctcggaa aacagcttgg tgcagccggc cgcgtgcagt tcggcccgtt ggttggtcaa
    20101 gtcctggtcg tcggtgctga cgcgggcata gcccagcagg ccagcggcgg cgctcttgtt
    20161 catggcgtaa tgtctccggt tctagtcgca agtattctac tttatgcgac taaaacacgc
    20221 gacaagaaaa cgccaggaaa agggcagggc ggcagcctgt cgcgtaactt aggacttgtg
    20281 cgacatgtcg ttttcagaag acggctgcac tgaacgtcag aagccgactg cactatagca
    20341 gcggaggggt tggatcaaag tactttgatc ccgaggggaa ccctgtggtt ggcatgcaca
    20401 tacaaatgga cgaacggata aaccttttca cgccctttta aatatccgat tattctaa
//
