LOCUS       sequence-486658-        9194 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used to grow up pseudotype virus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9194)
  AUTHORS   Reardon TR, Murray AJ, Turi GF, Wirblich C, Croce KR, Schnell MJ, 
            Jessell TM, Losonczy A
  TITLE     Rabies Virus CVS-N2c Strain Enhances Retrograde Synaptic Transfer 
            and Neuronal Viability.
  JOURNAL   Neuron. 2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004.
  PUBMED    26804990
REFERENCE   2  (bases 1 to 9194)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Jan 19. pii: S0896-6273(16)00005-2. doi: 
            10.1016/j.neuron.2016.01.004."
COMMENT     Sequence Label: pCMMP-EnvA_CVS-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..9194
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        85..388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        389..592
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     543..563
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     misc_feature    814..1171
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1236..1652
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1582..1604
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1620..1636
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     1974..1990
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     regulatory      2053..2062
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     3903..3919
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3903..3919
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(3953..3969)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3954..3970)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     misc_feature    3999..4565
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    4015..4567
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(4159..4176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     4386..4405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(4579..4581,4582..4584,4585..5298)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4624..4645)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4885..4904)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5232..5253
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(6575..6591)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6575..6591)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6588..6610)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6599..6615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6623..6629,6630..6647,6648..6653))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6668..6689
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6806..6823)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6977..7565)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7057..7076)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7736..8527,8528..8596))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8359..8378
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8597..8701)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8769..8787
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8825..8847)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8947..8966
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9160..9182
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9174..9191
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9175..9191
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactagtta ttaatagtaa tcaattacgg ggtcattagt
       61 tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg
      121 accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc
      181 aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc
      241 agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg
      301 gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat
      361 ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg
      421 tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag
      481 tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt
      541 gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctcgtttagt
      601 gaaccggcgc cagtcctccg atagactgcg tcgcccgggt acccgtgttc tcaataaacc
      661 ctcttgcagt tgcatccgac tcgtggtctc gctgttcctt gggagggtct cctctgagtg
      721 attgactacc cgtcagcggg gtctttcatt tggaggttcc accgagattt ggagacccct
      781 gcccagggac caccgacccc cccgccggga ggtaagctgg ccagcaactt atctgtgtct
      841 gtccgattgt ctagtgtcta tgactgattt tatgcgcctg cgtcggtact agttagctaa
      901 ctagctctgt atctggcgga cccgtggtgg aactgacgag ttcggaacac ccggccgcaa
      961 ccctgggaga cgtcccaggg acttcggggg ccgtttttgt ggcccgacct gagtcctaaa
     1021 atcccgatcg tttaggactc tttggtgcac cccccttaga ggagggatat gtggttctgg
     1081 taggagacga gaacctaaaa cagttcccgc ctccgtctga atttttgctt tcggtttggg
     1141 accgaagccg cgccgcgcgt cttgtctgct gcagcatcgt tctgtgttgt ctctgtctga
     1201 ctgtgtttct gtatttgtct gaaaatatgg gcccgggcta gactgttacc actcccttaa
     1261 gtttgacctt aggtcactgg aaagatgtcg agcggatcgc tcacaaccag tcggtagatg
     1321 tcaagaagag acgttgggtt accttctgct ctgcagaatg gccaaccttt aacgtcggat
     1381 ggccgcgaga cggcaccttt aaccgagacc tcatcaccca ggttaagatc aaggtctttt
     1441 cacctggccc gcatggacac ccagaccagg tcccctacat cgtgacctgg gaagccttgg
     1501 cttttgaccc ccctccctgg gtcaagccct ttgtacaccc taagcctccg cctcctcttc
     1561 ctccatccgc cccgtctctc ccccttgaac ctcctcgttc gaccccgcct cgatcctccc
     1621 tttatccagc cctcactcct tctctaggcg cccccatatg gccatatgag atcttatatg
     1681 gggcaccccc gccccttgta aacttccctg accctgacat gacaagagtt actaacagcc
     1741 cctctctcca agctcactta caggctctct acttagtcca gcacgaagtc tggagacctc
     1801 tggcggcagc ctaccaagaa caactggacc gaccggtggt acctcaccct taccgagtcg
     1861 gcgacacagt gtgggtccgc cgacaccaga ctaagaacct agaacctcgc tggaaaggac
     1921 cttacacagt cctgctgacc acccccaccg ccctcaaagt agacggcatc gcagcttgga
     1981 tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg accatcctct agcgagctcc
     2041 accgcggtgg cggccgccat ggaagccgtc ataaaggcat ttctgactgg gcaccctgga
     2101 aaggtgagca agaaggactc caagaagaag ccgccagcaa caagcaagaa agacccggag
     2161 aagacgccct tgctgccaac gagagttaat tatattattg gtgtcctggt cttgtgtgag
     2221 gtaacggggg taagagctga tgtccactta ctcgagcagc cagggaacct ttggattaca
     2281 tgggccaacc gtacaggcca aacggatttt tgcctctcta cacagtcagc cacctcccct
     2341 tttcaaacat gtttgatagg tatcccgtcc cctatttccg agggtgattt taagggatat
     2401 gtttctgata caaattgcac caccttggga actgatcggt tagtctcgtc agccgacttt
     2461 actggcggac ctgacaacag taccaccctc acttatcgga aggtctcatg cttgttgtta
     2521 aagctgaatg tctctatgtg ggatgagcca cctgaactac agctgttagg ttcccagtct
     2581 ctccctaaca ttactaatat tgctcagatt tccggtataa ccgggggatg cgtaggcttc
     2641 agaccacaag gggttccttg gtatctaggt tggtctagac aggaggccac gcggtttctc
     2701 cttagacacc cctctttctc taaatccacg gaaccgttta cggtggtgac agcggatagg
     2761 cacaatcttt ttatggggag tgagtactgc ggtgcatatg gctacagatt ttggaacatg
     2821 tataactgct cacaggtggg gcggcagtac cgctgtggta atgcgcgcag cccccgcccg
     2881 ggtcttcctg aaatccagtg tacaaggaga ggaggcaaat gggttaatca atcacaggaa
     2941 attaatgagt cggagccgtt cagctttacg gtgaactgta cagctagtag tttgggtaat
     3001 gccagtgggt gttgcggaaa agcaggcacg attctcccgg gaaagtgggt cgacagcaca
     3061 caaggtagtt tcaccaaacc aaaagcgcta ccacccgcaa ttttcctcat ttgtggggat
     3121 cgcgcatggc aaggaattcc cagtcgtccg gtagggggcc cctgctattt aggcaagctt
     3181 accatgttag cacctaagca tacagatatt ctcaaggtgc ttgtcaattc atcgcggaca
     3241 ggtataagac gtaaacgaag cacctcacac ctggatgata catgctcaga tgaagtgcag
     3301 ctttggggtc ctacagcaag aatctttgca tctatcctag ccccgggggt agcagctgcg
     3361 caagccttaa gagaaattga gagactagcc tgttggtccg ttaaacaggc taacttgaca
     3421 acatcactcc tcggggactt attggatgat gtcacgagta ttcgacacgc ggtcctgcag
     3481 aaccgagcgg ctattgactt cttgctccta gctcacggcc atggctgtga ggacgttgcc
     3541 ggaatgtgct gtttcaattt gagtgatcac agtgagtcta tacagaagaa gttccagcta
     3601 atgaaggaac atgtcaataa gatcggcgtg gacagcgacc caattggaag ttggctgcga
     3661 ggactattcg ggggaatagg agaatgggcc gttcatttgc tgaaaggact gcttttgggg
     3721 cttgtagtta ttttgttgct agtagtgtgc ctgccttgca gaagagccaa tcgaccagaa
     3781 tcgaaacaac gcagttttgg agggacaggg gggaatgtgt cagtcacttc ccaaagcgga
     3841 aaagtcatac cttcatggga atcatataag agtggaggtg agatcagact gtaagcggcc
     3901 gctctagaac tagtggatcc cccgggctgc aggaattcga tatcaagctt atcgataccg
     3961 tcgacctcga gggggggccc ggtacccaga tccggaattc cgcccccccc cctaacgtta
     4021 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
     4081 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
     4141 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
     4201 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
     4261 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
     4321 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
     4381 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
     4441 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
     4501 taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat
     4561 gataatatgg ccacaaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc
     4621 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc tggcgagggc
     4681 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg
     4741 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc
     4801 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc
     4861 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag
     4921 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac
     4981 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg
     5041 gccgacaagc agaagaacgg catcaaggcg aacttcaaga tccgccacaa catcgaggac
     5101 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg
     5161 ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga ccccaacgag
     5221 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg
     5281 gacgagctgt acaagtaatg aattaattaa gaattccgga ttagtccaat ttgttaaaga
     5341 caggatatca gtggtccagg ctctagtttt gactcaacaa tatcaccagc tgaagcctat
     5401 agagtacgag ccatagataa aataaaagat tttatttagt ctccagaaaa aggggggaat
     5461 gaaagacccc acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg
     5521 gaaaatacat aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag
     5581 aatatgggcc aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa
     5641 cagttggaac agcagaatat gggccaaaca ggatatctgt ggtaagcagt tcctgccccg
     5701 gctcagggcc aagaacagat ggtccccaga tgcggtcccg ccctcagcag tttctagaga
     5761 accatcagat gtttccaggg tgccccaagg acctgaaatg accctgtgcc ttatttgaac
     5821 taaccaatca gttcgcttct cgcttctgtt cgcgcgcttc tgctccccga gctcaataaa
     5881 agagcccaca acccctcact cggcgcgcca gtcctccgat agactgcgtc gcccgggtac
     5941 ccgtgttctc aataaaccct cttgcagttg catccgactc gtggtctcgc tgttccttgg
     6001 gagggtctcc tctgagtgat tgactacccg tcagcggggg tctttcagtt tctcccacct
     6061 acacaggtct cactaacatt cctgatgtgc cgcagggact ccgtcagccc ggtttttgtt
     6121 tataataaaa tgcaagaaca gtgttccctt caagccagac tacatcctga ctctcggctt
     6181 tataaaagaa tgttgaaggg ctctgtggac tatctgccac acgactttta agatttttat
     6241 gcctcctgga tgagggattt agtcaatcta tcctcgtcta ttttgctggc ttctccgtat
     6301 tttaaatttc tagtttgcac tcccttcctg agagcacggc gattgcagag tagttaatac
     6361 tctgagggca ggcttctgtg aaaaggttgc ctgggctcag tgtgagattt tgccataaaa
     6421 aggggtcctg cccctgtgta cagacagatc ggaatctaga gtgcatactc agagtccccg
     6481 cggttccggg gctctgatct cagggcatct ttgcctagag atcctctacg ccggacgcat
     6541 cgtggccggg taccgagctc gaattcgtaa tcatgtcata gctgtttcct gtgtgaaatt
     6601 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
     6661 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
     6721 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
     6781 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc
     6841 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg
     6901 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg
     6961 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac
     7021 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg
     7081 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct
     7141 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg
     7201 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct
     7261 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac
     7321 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt
     7381 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc
     7441 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca
     7501 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat
     7561 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac
     7621 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     7681 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     7741 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     7801 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     7861 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     7921 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     7981 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     8041 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     8101 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     8161 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     8221 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     8281 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     8341 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     8401 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     8461 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     8521 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     8581 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     8641 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     8701 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     8761 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg
     8821 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8881 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta
     8941 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     9001 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact
     9061 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
     9121 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     9181 cgacggccag tgcc
//
