LOCUS       sequence-486667-        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nonribosomal peptide synthetase from Streptomyces venezuluelae for 
            the production of watasemysin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Ronnebaum TA, Meneely KM, Horsman GP, Prisinzano TE, Lamb AL
  TITLE     Stuffed Epimerase Domains of Pyochelin Biosynthesis are Defunct 
            Methyltransferases.
  JOURNAL   Biochemistry. 2025 Sep 16;64(18):3919-3934. doi: 
            10.1021/acs.biochem.5c00172. Epub 2025 Aug 26.
  PUBMED    40857142
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1021/acs.biochem.5c00172"; journalName: "Biochemistry"; date: 
            "2025-09-16- 16"; volume: "64"; issue: "18"; pages: "3919-3934"
COMMENT     Sequence Label: SvPchE
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             4770..4792
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    4807..4831
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4831..4850)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4832..4850)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4938..4957)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        5163..5240
                     /gene="lacI"
                     /label=lacI promoter
     CDS             5241..6323
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(5260..5279)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             7132..7323
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(7317..7339)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7425..7567
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7480..7499
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7582..7599)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7753..8341)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7833..7852)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(8452..8471)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             8463..9278
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             8463..9278
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(8536..8555)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(9371..9826)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9508..9529)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9720..9739
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtagttcg ccctcttcat
      181 attcaactaa ctcgtcgctc ggatcacccg gcgcgccggc acgcgcatca ccacccgggc
      241 cgccatcaat accgcttgct tcccgcaact cacccacaag tgcgctcata cctgcaacgg
      301 tcggcgcacg cagcacatca cgcatgggca gttccacacc cagccgtcca cggatcgcag
      361 caacaagacg ggtcgccgtc aggctatcac cgcctagagc gaaaaagccg gcaccgcgcg
      421 cagtaactgg gacgccgagg acgcgcgccc ataactccgc caccgcctcc tccacagggc
      481 ctctcggagg gtcgtccaca ccctccgctg gagctgcagc tgcacgcaac gcttgttcca
      541 cgcgtctgcg atcaacttta ccattggcag acagcggcaa ctctgccagg ccaaccaggg
      601 atcccggaat catgtgggcc ggaagacgat cagccagcca agaacgcagc gccgctggcc
      661 gcgaagctga cgctggctcc gctgccggac ggcgcgcggt aattaaagcc acgccatcgg
      721 gctcccgcag ggtgacttca cggtcggtga agcctgccag ttccaggcta cgcagccagc
      781 gaggggcatc agtggctggg ctcggcgtcg caacggttgg gatggcaaca ggcgacgcgg
      841 tctcacgaac cagcaggcat aagcggccac ccgggcgcgc catcagagcc agcacacgcg
      901 cggcagcatc cggctctggt tcacggccca aaacatcgtt aacaaccagc aggtcgaaag
      961 cggcaaggcg cggctccggt actgcgaaag catcctgtct ggccgcacgc accggatgac
     1021 ccctccccgc cagacggtct tcagccgcgg taacacgtgc cgcgcgaggg tccagcaggg
     1081 tatagtcaac ggtgcccggt ttgatacggt caagcagtgc ggccgcgtta cggccacctg
     1141 cgcctgccca ctccgcgacg tccaatggac gcgctgcctc gcggtgcaga gctgcaactt
     1201 gctcggcaac cgcatccagg catgcttcag tacccgggag ctgtaatgcc aggccatccg
     1261 gggacaacac cgggtcgtcg tctaacgcat ccaacggtgc cgtacccgcc agcaccgcag
     1321 aaaggagtgg gagggcacct gcccaagcat ccaccagcgg ttgcagcgcc gttcctgcaa
     1381 tacgtcccct tagcgcacct gcgcgctcac cgtcgcgaac cgtaccccag cgcgggccac
     1441 gggtcagcgt accgtccggg ccacccagaa catttcggct acgcaggaac tccagccagc
     1501 tcgttatcag ctcacgattt ggcgccgcaa cgtgcagcgg cgtcggttca gccggtcccg
     1561 gctcgcctgg cgcgccctgt ggcgggccga gcagcggagc cagaagtgca gccagaaatg
     1621 tctccgccag cgctgcttca tcttcggcag actctaacgc cgcacccgat gcctgcaggg
     1681 cgctggcttg cacatcagct gcgaccggcg ccaccagcgc aaccggggag accgggctta
     1741 ccggggtcac cgggttcacc ggggttgccg ggttgaccgg ggccagaaac gcagccgcga
     1801 tctcacgggt gcggtcacca acaacggccg cgagtgcgtg gctgaccgcg gggtgcgttt
     1861 ggagcacaga ttcaatctcg ccaagttcca cacggaagcc gttgatcttc acttggtgat
     1921 cgatgcggcc caggaactcc agggtaccgt ccggccaata acggccttgg tctccggtac
     1981 gataccagcg tccatccggc gcgaccggaa aacgttcggc ggtcagggtg gagtcaccac
     2041 ggtatcctct agcgacgccc gtaccgccaa tccataattc acccggaacc cagtccggac
     2101 agtctctacc ataagggtca accacccggt aacgctggtt tgcgagggga cggccatacg
     2161 gaacgctacg ccaatgcggc ggaacctcgg ccacttcgta tgcatttgac cagattgcag
     2221 cctcggtcgc gcccccgaga gcaaccagtc tgcatcgacc accactagca cgagccaggc
     2281 ggcccggcag gtccagtccg atccaatcac cagacagcag cgccaggcgc agagtctccg
     2341 gtagcggcga gccatcggac gcggtaagca gcatatccat cagcgccgga acgctattcc
     2401 aaacagtcac tgcatgacgt tcgcaaagcg cgcgccaacg atgcgcgtca cgacgatctt
     2461 cttcgtctac cagcacaagt gccccgccct ctgccagcgg accgaacaca tcccaaacgg
     2521 acaggtcgaa gtccagtgct gaaacagcca gaacgcgatc ccgcgggccg atgccgtaac
     2581 gttcatcgat gtcctcgacg gtgttaaccg cagctcggtg gctgatctcc acgcccttgg
     2641 gggtaccggt ggagccactg gtaaatataa cgtacgcggt ctcttcacca ctgaggcgaa
     2701 ccggtgcgtc caacggggcc gctcgcaatg cttcggcaag cggcaaacgc ggcggacccg
     2761 cttcttgttc cggagcggtg cccgtaccgt ctaacaccag agcgcaacgg ctcagcgcgt
     2821 gaatacggtc acgacgctca ggcggctgat caacacctac cggcacatac gttcctcctg
     2881 ccgccaggac gcccagtacc gccgcaattt gatccggacc ctttggcgca gttacaacca
     2941 ccgggctgcc cggacgcact ccacgagccg ccagcgcgcc tgccacacgc agcgcgcgat
     3001 cagccagttc accgtgagtt agacggctat cctcacccca cagcagagcc ggggctgccg
     3061 cgcgcgccgc cgcacgttcg aagaacgcct ggtgtagcaa gcgaccgctt tcctctcgtg
     3121 ttacggagtt cacctctgca cggcaacgaa actgggatgc cggcagcggg atatccggcg
     3181 ggcgggtcca atcagcacga gcgagacctc tcagaagctc ggtgtaggcg gtaaacatgg
     3241 tatcaatcac accatccggg aacagggcct cgactgcatc ccaggccagc agcaggccac
     3301 cgtcgcgagt accatagacc tggtggtcca accaaacctg tggggtttgg ctaaccatcc
     3361 agctcagttc accaaagcga gccgcgaagt cgtcagggac taacgggctt tccaggttac
     3421 acgcgaaaac aaccggggcg gtgcgagggg taccggtatc ggttctaaca aagtcacgca
     3481 gcacatcaac accggtgtac gccgcatgac ccacatcttc gtgtaagcgg cgctggatcg
     3541 cgcgcgcgcg atcggcaaag ccgtcctcgc ccgccagacg aacgtctaac aacaacaggg
     3601 aggtgaagtc cgcaacaata cgcgcgatgt ccgggtggat accttggtca cggtcgaaca
     3661 acggaatatt caacagaaaa tgctgctgac cgctccaacg cgccagaatt tcgctgaagg
     3721 ctgtcaccaa caggaccgcc ggtgtgacac catgttctgc tgcacgctga cgcagagcgg
     3781 tccattcttc acgagacagg gtgtgatccc gacgcacgaa gcgcggtgca ccgatatcgc
     3841 ctgggtccac cgccagcggt aacgccggac cacccggcag tgccggtaaa cgacgatgcc
     3901 aatgcgcacg agcacgctca cgatccgcgg tgcgacgagc agcggtatcc gccaggtaac
     3961 gctgaaaacc gtaacccagc tctgcaacgc cttccgggtc tgcataaagg gcggccagat
     4021 cgctaaggag cagacgaatg ctatgaacgt ccgcaactaa caaatcggcg ttcacgtgca
     4081 gacgctgatt gccgtccggc agacgagaca gttggacatc gaacacctca ccacgtgcga
     4141 catctaacac cctgtgggag aggcgatcgc gcaatcttgc cagcgcctcc tccccgtcgc
     4201 ccggcaggtc agccagatga tgaaccgtca ggccaggcca agtaatacgc tcgccggtcc
     4261 actgggtacc gtcgtcggca aaggacgcac gcagcatcgg gtggcgacgc atcaaggcca
     4321 gcaccgcaga ctccaaacga tcgggcgcca cgtcacgggt gtcgatctcc aggtaggcat
     4381 gacaaccgac accgcccaac ggccggtcgg tcccccgacc aatccagtat gcttgctgca
     4441 cagcggtcag gccgaagggt gcgtcggcat caaacggcgc cgccgcgctg gtgtccgccg
     4501 gttccggtgc gccctccggc tccgggcgac ggcccgtcaa cagcgcttcc caaactctaa
     4561 ggcgcggatc tttcgccatt tccgtgaagt ccacaccagc gccggagcgg ttcagacggt
     4621 taaccagggt catcagttgc aggctgtgga gacccagttc aaacaggttc gcgtcctcat
     4681 catccggacc gagctctacg cccaacaacg ccgtcagttc ttccctcagg tctacgggtt
     4741 gtccagctgt tgacctactc atatgtatat ctccttctta aagttaaaca aaattatttc
     4801 tagaggggaa ttgttatccg ctcacaattc ccctatagtg agtcgtatta atttcgcggg
     4861 atcgagatcg atctcgatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     4921 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     4981 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     5041 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     5101 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgagatcc
     5161 cggacaccat cgaatggcgc aaaacctttc gcggtatggc atgatagcgc ccggaagaga
     5221 gtcaattcag ggtggtgaat gtgaaaccag taacgttata cgatgtcgca gagtatgccg
     5281 gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa
     5341 cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta cattcccaac cgcgtggcac
     5401 aacaactggc gggcaaacag tcgttgctga ttggcgttgc cacctccagt ctggccctgc
     5461 acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg
     5521 tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc
     5581 ttctcgcgca acgcgtcagt gggctgatca ttaactatcc gctggatgac caggatgcca
     5641 ttgctgtgga agctgcctgc actaatgttc cggcgttatt tcttgatgtc tctgaccaga
     5701 cacccatcaa cagtattatt ttctcccatg aagacggtac gcgactgggc gtggagcatc
     5761 tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg cccattaagt tctgtctcgg
     5821 cgcgtctgcg tctggctggc tggcataaat atctcactcg caatcaaatt cagccgatag
     5881 cggaacggga aggcgactgg agtgccatgt ccggttttca acaaaccatg caaatgctga
     5941 atgagggcat cgttcccact gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa
     6001 tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga catctcggta gtgggatacg
     6061 acgataccga agacagctca tgttatatcc cgccgttaac caccatcaaa caggattttc
     6121 gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc caggcggtga
     6181 agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata
     6241 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca cgacaggttt
     6301 cccgactgga aagcgggcag tgagcgcaac gcaattaatg taagttagct cactcattag
     6361 gcaccgggat ctcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg
     6421 gcgcggggca tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta
     6481 ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg
     6541 acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc
     6601 gtcactggtc ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg
     6661 gccccacggg tgcgcatgat cgtgctcctg tcgttgagga cccggctagg ctggcggggt
     6721 tgccttactg gttagcagaa tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc
     6781 tgcaaaacgt ctgcgacctg agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa
     6841 agtctggaaa cgcggaagtc agcgccctgc accattatgt tccggatctg catcgcagga
     6901 tgctgctggc taccctgtgg aacacctaca tctgtattaa cgaagcgctg gcattgaccc
     6961 tgagtgattt ttctctggtc ccgccgcatc cataccgcca gttgtttacc ctcacaacgt
     7021 tccagtaacc gggcatgttc atcatcagta acccgtatcg tgagcatcct ctctcgtttc
     7081 atcggtatca ttacccccat gaacagaaat cccccttaca cggaggcatc agtgaccaaa
     7141 caggaaaaaa ccgcccttaa catggcccgc tttatcagaa gccagacatt aacgcttctg
     7201 gagaaactca acgagctgga cgcggatgaa caggcagaca tctgtgaatc gcttcacgac
     7261 cacgctgatg agctttaccg cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7321 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7381 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7441 tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     7501 tgagagtgca ccatatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg
     7561 catcaggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
     7621 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
     7681 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     7741 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     7801 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     7861 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     7921 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     7981 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     8041 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     8101 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     8161 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     8221 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     8281 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     8341 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     8401 agggattttg gtcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg
     8461 ttatgagcca tattcaacgg gaaacgtctt gctctaggcc gcgattaaat tccaacatgg
     8521 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa
     8581 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta
     8641 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc
     8701 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg
     8761 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata
     8821 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc
     8881 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt
     8941 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga
     9001 aagaaatgca taaacttttg ccattctcac cggattcagt cgtcactcat ggtgatttct
     9061 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag
     9121 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt
     9181 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata
     9241 aattgcagtt tcatttgatg ctcgatgagt ttttctaaga attaattcat gagcggatac
     9301 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     9361 gtgccacctg aaattgtaaa cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     9421 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     9481 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     9541 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     9601 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     9661 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     9721 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     9781 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgcca
//
