LOCUS       sequence-486677-        4385 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Myo-inositol-1(or 4)-monophosphatase from Aquifex aeolicus.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4385)
  AUTHORS   Hoffpauir ZA, Lamb AL
  TITLE     Identification of the phosphatase essential for riboflavin 
            biosynthesis in Aquifex aeolicus.
  JOURNAL   J Biol Chem. 2025 May;301(5):108443. doi: 10.1016/j.jbc.2025.108443.
            Epub 2025 Mar 25.
  PUBMED    40147773
REFERENCE   2  (bases 1 to 4385)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2025-05"; volume: "301(5):108443. doi"; pages: " 
            10.1016/j.jbc.2025.108443. Epub 2025 Mar 25"
COMMENT     Sequence Label: AaIMP
FEATURES             Location/Qualifiers
     source          1..4385
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(26..73)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     69..87
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(140..157)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             966..988
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(1019..1038)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1020..1038)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1547..1738
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(1732..1754)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    1840..1982
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     1895..1914
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(1997..2014)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2168..2756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2248..2267)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2927..3718,3719..3787))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3550..3569
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3788..3892)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(3919..4374)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4056..4077)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4268..4287
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
       61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
      121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagttacaca gcgacctcca
      181 tgtatttttt cgctacctgc agaatgaaat cgtgcagtgc tttgtttccc gcgataatat
      241 cgctcacacc aaacggctcg cccaccaggg tgtaaacacc gcctgcctcc ttaaggataa
      301 ccaaacccgc ggtaatatcc cacggtttca tttcaaattc catcatgccg tcgaaaatgc
      361 cttccgcaac catgcacagg tccaccgccg ccgcacccgg acgacgcata gacccaactt
      421 cataaaaaac gtctttgaaa atattcaagt aaatgctgat gtcacggcgg ctacggcttg
      481 ggaagccgta cacgacgccc gcgtgcttca ggctttcgtt gtccttcacc ttgatgcgtt
      541 taccattcac atacgcaccc aaacctttgg caccccaata cagtttatca aagtacggca
      601 ggtaaaccgc accaacaatc ggttcctcac ccttaaccaa gcctacagac actgcaaaga
      661 tcgggaagcc gttgatgtag tttttcgtac catccagcgg atcgataaac caacgatatt
      721 ccgaaccgga accctcggca cccatctcct cacccaccac ttcatgatcc gggaagaact
      781 tgaggatgac ctccttgatg cgctcttcgc tggttttgtc aacataggac acgaagtctt
      841 tctcgccctt ctcttcaata ttttccttct taactttgcc aaagttctcc ttcagaactt
      901 gaccgccagc cagggcagcg atcttagcta cttctaaata tttctttaga ttctccatat
      961 gtatatctcc ttcttaaagt taaacaaaat tatttctaga gggaaaccgt tgtggtctcc
     1021 ctatagtgag tcgtattaat ttcgcgggat cgagatctcg ggcagcgttg ggtcctggcc
     1081 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct
     1141 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa
     1201 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct
     1261 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg
     1321 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt
     1381 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag
     1441 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg
     1501 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga
     1561 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa
     1621 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc
     1681 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca
     1741 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc
     1801 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg
     1861 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga
     1921 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca
     1981 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     2041 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     2101 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     2161 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     2221 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     2281 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     2341 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     2401 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     2461 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     2521 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     2581 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     2641 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     2701 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     2761 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     2821 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     2881 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     2941 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     3001 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     3061 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     3121 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     3181 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg
     3241 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     3301 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     3361 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     3421 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     3481 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt
     3541 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac
     3601 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     3661 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     3721 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     3781 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     3841 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     3901 cccgaaaagt gccacctgaa attgtaaacg ttaatatttt gttaaaattc gcgttaaatt
     3961 tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc ccttataaat
     4021 caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag agtccactat
     4081 taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac
     4141 tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc
     4201 ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg aacgtggcga
     4261 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca
     4321 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt
     4381 cgcca
//
