LOCUS       sequence-486867-        9586 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  HNRNPD Knockout.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Kumar V, Mittal A, Kumar A, Lone MR, Yadav V, Verma P, Pradhan D, 
            Mishra D, Thakar A, Chauhan SS
  TITLE     NF-kappa-B mediates transactivation of HNRNPD, resulting in PTEN 
            destabilization and constitutive activation of the PI3K-AKT pathway 
            in oral cancer cells
  JOURNAL   bioRxiv 2025.01.05.631360
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.01.05.631360"
COMMENT     Sequence Label: pDG459-HNRNPD-KO
FEATURES             Location/Qualifiers
     source          1..9586
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        120..405
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
     misc_RNA        complement(502..577)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(599..847)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     rep_origin      complement(909..1497)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(989..1008)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1668..2459,2460..2528))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2291..2310
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2529..2633)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2701..2719
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2757..2779)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2879..2898
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(2915..3370)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3052..3073)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3264..3283
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   3445..3585
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3456..3585
                     /label=AAV2 ITR
     promoter        3594..3842
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     misc_RNA        3864..3939
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     primer_bind     complement(3982..3998)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3982..3998)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    4007..4214
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     4203..4220
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(4248..4847)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4331..4351)
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     primer_bind     4828..4847
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     CDS             complement(4848..4901)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(4917..4964)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             complement(4965..9065)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(4965..9065)
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
                     LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
                     LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
                     RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
                     NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
                     GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
                     QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
                     KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
                     SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
                     TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
                     VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
                     KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
                     LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
                     KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
                     YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
                     EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
                     AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
                     AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
                     ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
                     SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
                     IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
                     ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
                     NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
                     ATLIHQSITGLYETRIDLSQLGGD"
     CDS             complement(9090..9110)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(9117..9182)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      9182..9191
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          9203..9430
                     /label=hybrid intron
                     /note="hybrid between chicken beta-actin (CBA) and minute 
                     virus of mice (MMV) introns (Gray et al., 2011)"
ORIGIN
        1 cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa atacaaaatt
       61 gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct cacctcgacc
      121 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
      181 atgtactggg cacaatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
      241 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
      301 cattgacgtc aatggaaagt ccctattggc gttactattg acgtcaatgg gcgggggtcg
      361 ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgggtac ctctagagcc
      421 atttgtctgc agaattggcg cacgcgctaa aaacggacta gccttatttt aacttgctat
      481 ttctagctct aaaacaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      541 tagccttatt ttaacttgct atttctagct ctaaaaccgc cgaactgctc ctccgaccgg
      601 tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg
      661 taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt
      721 attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc
      781 caattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata
      841 ggccctcaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
      901 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
      961 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     1021 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     1081 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     1141 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     1201 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     1261 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     1321 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     1381 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     1441 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     1501 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     1561 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     1621 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
     1681 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
     1741 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
     1801 ataccgcggc ttccacgctc accggctcca gatttatcag caataaacca gccagccgga
     1861 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
     1921 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
     1981 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
     2041 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
     2101 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
     2161 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
     2221 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
     2281 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
     2341 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
     2401 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
     2461 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
     2521 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
     2581 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
     2641 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa
     2701 aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc
     2761 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     2821 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg
     2881 gcatcagagc agattgtact gagagtgcac cataaaattg taaacgttaa tattttgtta
     2941 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaatagac cgaaatcggc
     3001 aaaatccctt ataaatcaaa agaatagccc gagatagagt tgagtgttgt tccagtttgg
     3061 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3121 cagggcgatg gcccactacg tgaaccatca cccaaatcaa gttttttggg gtcgaggtgc
     3181 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     3241 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc taaggcgctg
     3301 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     3361 cagggcgcgt actatggttg ctttgacgta tgcggtgtga aataccgcac agatgcgtaa
     3421 ggagaaaata ccgcatcagg cgcccctgca ggcagctgcg cgctcgctcg ctcactgagg
     3481 ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca gtgagcgagc
     3541 gagcgcgcag agagggagtg gccaactcca tcactagggg ttcctgcggc cgcgagggcc
     3601 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     3661 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     3721 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     3781 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     3841 ccgctgaagc agcgacggca caggttttag agctagaaat agcaagttaa aataaggcta
     3901 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct tttttcctgc aggattcgta
     3961 tcgaattcct gcagcccggg ggatccacta gttctagagc ggccgctccc cagcatgcct
     4021 gctattctct tcccaatcct cccccttgct gtcctgcccc accccacccc ccagaataga
     4081 atgacaccta ctcagacaat gcgatgcaat ttcctcattt tattaggaaa ggacagtggg
     4141 agtggcacct tccagggtca aggaaggcac gggggagggg caaacaacag atggctggca
     4201 actagaaggc acagtcgagg ctgatcagcg agctctagtt agaattctca ggcaccgggc
     4261 ttgcgggtca tgcaccaggt gcgcggtcct tcgggcacct cgacgtcggc ggtgacggtg
     4321 aagccgagcc gctcgtagaa ggggaggttg cggggcgcgg aggtctccag gaaggcgggc
     4381 accccggcgc gctcggccgc ctccactccg gggagcacga cggcgctgcc cagacccttg
     4441 ccctggtggt cgggcgagac tccgacggtg gccaggaacc acgcgggctc cttgggccgg
     4501 tgcggcgcca ggaggccttc catctgttgc tgcgcggcca gccgggaacc gctcaactcg
     4561 gccatgcgcg ggccgatctc ggcgaacacc gcccccgctt cgacgctctc cggcgtggtc
     4621 cagaccgcca ccgcggcgcc gtcgtccgcg acccacacct tgccgatgtc gagcccgacg
     4681 cgcgtgagga agagttcttg cagctcggtg acccgctcga tgtggcggtc cggatcgacg
     4741 gtgtggcgcg tggcggggta gtcggcgaac gcggcggcga gggtgcgtac ggccctgggg
     4801 acgtcgtcgc gggtggcgag gcgcaccgtg ggcttgtact cggtcattgg gccaggattc
     4861 tcctcgacgt caccgcatgt tagcagactt cctctgccct ctccactgcc gaattccttt
     4921 ttcttttttg cctggccggc ctttttcgtg gccgccggcc ttttgtcgcc tcccagctga
     4981 gacaggtcga tccgtgtctc gtacaggccg gtgatgctct ggtggatcag ggtggcgtcc
     5041 agcacctctt tggtgctggt gtacctcttc cggtcgatgg tggtgtcaaa gtacttgaag
     5101 gcggcagggg ctcccagatt ggtcagggta aacaggtgga tgatattctc ggcctgctct
     5161 ctgatgggct tatcccggtg cttgttgtag gcggacagca ctttgtccag attagcgtcg
     5221 gccaggatca ctctcttgga gaactcgctg atctgctcga tgatctcgtc caggtagtgc
     5281 ttgtgctgtt ccacaaacag ctgtttctgc tcattatcct cgggggagcc cttcagcttc
     5341 tcatagtggc tggccaggta caggaagttc acatatttgg agggcagggc cagttcgttt
     5401 cccttctgca gttcgccggc agaggccagc attctcttcc ggccgttttc cagctcgaac
     5461 agggagtact taggcagctt gatgatcagg tcctttttca cttctttgta gcccttggct
     5521 tccagaaagt cgatgggatt cttctcgaag ctgcttcttt ccatgatggt gatccccagc
     5581 agctctttca cactcttcag tttcttggac ttgccctttt ccactttggc caccaccagc
     5641 acagaatagg ccacggtggg gctgtcgaag ccgccgtact tcttagggtc ccagtccttc
     5701 tttctggcga tcagcttatc gctgttcctc ttgggcagga tagactcttt gctgaagccg
     5761 cctgtctgca cctcggtctt tttcacgata ttcacttggg gcatgctcag cactttccgc
     5821 acggtggcaa aatcccggcc cttatcccac acgatctccc cggtttcgcc gtttgtctcg
     5881 atcagaggcc gcttccggat ctcgccgttg gccagggtaa tctcggtctt gaaaaagttc
     5941 atgatgttgc tgtagaagaa gtacttggcg gtagccttgc cgatttcctg ctcgctcttg
     6001 gcgatcatct tccgcacgtc gtacaccttg tagtcgccgt acacgaactc gctttccagc
     6061 ttagggtact ttttgatcag ggcggttccc acgacggcgt tcaggtaggc gtcgtgggcg
     6121 tggtggtagt tgttgatctc gcgcactttg taaaactgga aatccttccg gaaatcggac
     6181 accagcttgg acttcagggt gatcactttc acttcccgga tcagcttgtc attctcgtcg
     6241 tacttagtgt tcatccggga gtccaggatc tgtgccacgt gctttgtgat ctgccgggtt
     6301 tccaccagct gtctcttgat gaagccggcc ttatccagtt cgctcaggcc gcctctctcg
     6361 gccttggtca gattgtcgaa ctttctctgg gtaatcagct tggcgttcag cagctgccgc
     6421 cagtagttct tcatcttctt cacgacctct tcggagggca cgttgtcgct cttgccccgg
     6481 ttcttgtcgc ttctggtcag caccttgttg tcgatggagt cgtccttcag aaagctctga
     6541 ggcacgatat ggtccacatc gtagtcggac agccggttga tgtccagttc ctggtccacg
     6601 tacatatccc gcccattctg caggtagtac aggtacagct tctcgttctg cagctgggtg
     6661 ttttccacgg ggtgttcttt caggatctgg ctgcccagct ctttgatgcc ctcttcgatc
     6721 cgcttcattc tctcgcggct gttcttctgt cccttctggg tggtctggtt ctctctggcc
     6781 atttcgatca cgatgttctc gggcttgtgc cggcccatca ctttcacgag ctcgtccacc
     6841 accttcactg tctgcaggat gcccttctta atggcggggc tgccggccag attggcaatg
     6901 tgctcgtgca ggctatcgcc ctggccggac acctgggctt tctggatgtc ctctttaaag
     6961 gtcaggctgt cgtcgtggat cagctgcatg aagtttctgt tggcgaagcc gtcggacttc
     7021 aggaaatcca ggattgtctt gccggactgc ttgtcccgga tgccgttgat cagcttccgg
     7081 ctcagcctgc cccagccggt gtatctccgc cgcttcagct gcttcatcac tttgtcgtcg
     7141 aacaggtggg cataggtttt cagccgttcc tcgatcatct ctctgtcctc aaacagtgtc
     7201 agggtcagca cgatatcttc cagaatgtcc tcgttttcct cattgtccag gaagtccttg
     7261 tccttgataa ttttcagcag atcgtggtat gtgcccaggg aggcgttgaa ccgatcttcc
     7321 acgccggaga tttccacgga gtcgaagcac tcgattttct tgaagtagtc ctctttcagc
     7381 tgcttcacgg tcactttccg gttggtcttg aacagcaggt ccacgatggc ctttttctgc
     7441 tcgccgctca ggaaggcggg ctttctcatt ccctcggtca cgtatttcac tttggtcagc
     7501 tcgttataca cggtgaagta ctcgtacagc aggctgtgct tgggcagcac cttctcgttg
     7561 ggcaggttct tatcgaagtt ggtcatccgc tcgatgaagc tctgggcgga agcgcccttg
     7621 tccaccactt cctcgaagtt ccagggggtg atggtttcct cgctctttct ggtcatccag
     7681 gcgaatctgc tgtttcccct ggccagaggg cccacgtagt aggggatgcg gaaggtcagg
     7741 atcttctcga tcttttcccg gttgtccttc aggaatgggt aaaaatcttc ctgccgccgc
     7801 agaatggcgt gcagctctcc caggtggatc tggtggggga tgctgccgtt gtcgaaggtc
     7861 cgctgcttcc gcagcaggtc ctctctgttc agcttcacga gcagttcctc ggtgccgtcc
     7921 atcttttcca ggatgggctt gatgaacttg tagaactctt cctggctggc tccgccgtca
     7981 atgtagccgg cgtagccgtt cttgctctgg tcgaagaaaa tctctttgta cttctcaggc
     8041 agctgctgcc gcacgagagc tttcagcagg gtcaggtcct ggtggtgctc gtcgtatctc
     8101 ttgatcatag aggcgctcag gggggccttg gtgatctcgg tgttcactct caggatgtcg
     8161 ctcagcagga tggcgtcgga caggttcttg gcggccagaa acaggtcggc gtactggtcg
     8221 ccgatctggg ccagcaggtt gtccaggtcg tcgtcgtagg tgtccttgct cagctgcagt
     8281 ttggcatcct cggccaggtc gaagttgctc ttgaagttgg gggtcaggcc caggctcagg
     8341 gcaatcaggt ttccgaacag gccattcttc ttctcgccgg gcagctgggc gatcagattt
     8401 tccagccgtc tgctcttgct cagtctggca gacaggatgg ccttggcgtc cacgccgctg
     8461 gcgttgatgg ggttttcctc gaacagctgg ttgtaggtct gcaccagctg gatgaacagc
     8521 ttgtccacgt cgctgttgtc ggggttcagg tcgccctcga tcaggaagtg gccccggaac
     8581 ttgatcatgt gggccagggc cagatagatc agccgcaggt cggccttgtc ggtgctgtcc
     8641 accagtttct ttctcaggtg gtagatggtg gggtacttct cgtggtaggc cacctcgtcc
     8701 acgatgttgc cgaagatggg gtgccgctcg tgcttcttat cctcttccac caggaaggac
     8761 tcttccagtc tgtggaagaa gctgtcgtcc accttggcca tctcgttgct gaagatctct
     8821 tgcagatagc agatccggtt cttccgtctg gtgtatcttc ttctggcggt tctcttcagc
     8881 cgggtggcct cggctgtttc gccgctgtcg aacagcaggg ctccgatcag gttcttcttg
     8941 atgctgtgcc ggtcggtgtt gcccagcacc ttgaatttct tgctgggcac cttgtactcg
     9001 tcggtgatca cggcccagcc cacagagttg gtgccgatgt ccaggccgat gctgtacttc
     9061 ttgtcggctg ctgggactcc gtggataccg accttccgct tcttctttgg ggccatctta
     9121 tcgtcatcgt ctttgtaatc aatatcatga tccttgtagt ctccgtcgtg gtccttatag
     9181 tccatggtgg caccggtcca acctgaaaaa aagtgatttc aggcaggtgc tccaggtaat
     9241 taaacattaa taccccacca accaaccatc ccttaaaccc ttacctcttg ctcagctaat
     9301 tacagcccgg aggagaaggg ccgtcccgcc cgctcacctg tgggagtaac gcggtcagtc
     9361 agagccgggg cgggcggcgc gaggcggcgg cggagcgggg cacggggcga aggcagcgcg
     9421 cagcgactcc cgcccgccgc gcgcttcgct ttttataggg ccgccgccgc cgccgcctcg
     9481 ccataaaagg aaactttcgg agcgcgccgc tctgattggc tgccgccgca cctctccgcc
     9541 tcgccccgcc ccgcccctcg ccccgccccg ccccgcctgg cgcscc
//
