LOCUS       sequence-486883-       15043 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  All-in-one subcellar localization vector. Construct containing EGFP 
            tag and cell nucleus-targeted mCherry for co-localization study in 
            plants..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15043)
  AUTHORS   Hao Y, Yuan Z, Li Y, Zuo D, Cheng H, Wang Q, Zhang Y, Lv L, Liu J, 
            Song G
  TITLE     An all-in-one plant virus-based vector toolkit for streamlined gene 
            silencing, overexpression, and genome editing.
  JOURNAL   Plant Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379.
  PUBMED    40394900
REFERENCE   2  (bases 1 to 15043)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Commun. 2025 May 19:101379. doi: 10.1016/j.xplc.2025.101379."
COMMENT     Sequence Label: pVTCL1
FEATURES             Location/Qualifiers
     source          1..15043
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    938..1111
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(1164..1874)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(1442..1461)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        complement(2323..2668)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     polyA_signal    2698..2872
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     CDS             complement(join(2880..3593,3594..3596,3597..3599))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(2883..3593,3594..3596,3597..3599))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(2925..2946)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     3274..3293
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3533..3554
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     promoter        complement(3683..4359)
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     complement(8129..8146)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8129..8145)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8138..8160)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    8348..8372
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             9673..10302
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             10731..11804
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      11870..12064
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(12300..12322)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    12408..12548
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     12463..12482
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(12563..12580)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12734..13322)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12814..12833)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(13409..14203)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     misc_feature    14628..14652
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     protein_bind    14920..14941
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(14956..14961,14962..14979,14980..14986)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    14994..15010
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     14999..15021
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     15018..15034
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15018..15034
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aattcgagct cattcgagct ccagaacgat ctcagttagg tcatgggaaa gctgatattc
       61 atccctctga gactctatat aattaaatgc gtttggggga ttagctaatt gagaatccat
      121 atagagaaat atggccgcgc agcgacgccg gttgaagaat atgaacaagg aataagatga
      181 acaaggaaga agatgaacaa gcaataattt aattaattat tgttaataag cataaattat
      241 gaaaataatt ttgatggtaa tgaaagtatt ataaataata tttaggagaa aatattgtta
      301 aggaaatatc tggttatgtt tatttgttaa ggtatatggg tttttataga aacccaggag
      361 cagctatggt aagaaaccca ggactcaaaa aatgcctctc aacttctctc atatgttgcg
      421 gagtctggag tcccatttat accatagaaa gcattggggg tatagcatgg taatttcaca
      481 tcggactcca taccgcggcc atctatataa tattactgat ggccgcgcga agtcccggta
      541 tgccttttca gccgttggat ctggagccgt acctttttcg cgttcccatt ttgcccctgg
      601 agtcccgagc tatttacgac cccctgccac cgagtcccct tcgtggagcg ctcttcgtcc
      661 aacacgtgtc ccaacttttg cgcatttttg actacctgtc gcgcacttga attaaagttg
      721 cccttttatt ttcttccaat tattttgcgg ctgagtagtc tagataattg tgacaactta
      781 gtgagcaagt tgtaggagga ccctatccgt ctgggaaaat aattgggttt tcacgtgtac
      841 caatgggaaa taatatgtat agcctattta atttagaaaa ttaaatagtt ttagtaattc
      901 ctatataata gcgtttacta gtctgggtaa aattcgtgat ctggatttta gtatggattt
      961 tggttttagg aattagaaat tttattgata gaagtatttt acaaatacaa atacatacta
     1021 agggtttctt atatgctcaa cacatgagcg aaaccctata ggaaccctaa ttcccttatc
     1081 tgggaactac tcacacatta ttatggagaa actcgagctt gtcgatcgac agatcccggt
     1141 cggcatctac tcggtaccct agattacttg tacagctcgt ccatgccgcc ggtggagtgg
     1201 cggccctcgg cgcgttcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
     1261 tccaacttga tgttgacgtt gtaggcgccg ggcagctgca cgggcttctt ggccttgtag
     1321 gtggtcttga cctcagcgtc gtagtggccg ccgtccttca gcttcagcct ctgcttgatc
     1381 tcgcccttca gggcgccgtc ctcggggtac atccgctcgg aggaggcctc ccagcccatg
     1441 gtcttcttct gcattacggg gccgtcggag gggaagttgg tgccgcgcag cttcaccttg
     1501 tagatgaact cgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
     1561 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt caagtagtcg
     1621 gggatgtcgg cggggtgctt cacgtaggcc ttggagccgt acatgaactg aggggacagg
     1681 atgtcccagg cgaagggcag ggggccaccc ttggtcacct tcagcttggc ggtctgggtg
     1741 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
     1801 ccctccatgt gcaccttgaa gcgcatgaac tccttgatga tggccatgtt atcctcctcg
     1861 cccttgctca ccatggatcc agaactcgta aacttcgtaa ccgccttagt cccttcagaa
     1921 acagcatgtt tcgccaactc tccaggcaac acaagtctca ccgcagtctg aatctcccta
     1981 gaagtaatcg tcggcttctt gttgtacctc gcaagcttcg aagactcacc agcaagtttc
     2041 tcaaagatat cattgatgaa actgttcatg attcccatgg ctttgctgga gattccgata
     2101 tctggatgaa cttgcttcaa caccttgaag atgtagatct tgtatgtctc aacgttcttc
     2161 tttgatctct tcttcttctt gtctccggcg ccggctggtt ccttcgggag tttctttccg
     2221 gcttttggtt tcttctctgc cgctgctgct ggttcggctg ccggagtttt ctctgccggt
     2281 ttcttctccg ctggtttctt atctgccttc gccatggtac cctgtcctct ccaaatgaaa
     2341 tgaacttcct tatatagagg aagggtcttg cgaaggatag tgggattgtg cgtcatccct
     2401 tacgtcagtg gagatatcac atcaatccac ttgctttgaa gacgtggttg gaacgtcttc
     2461 tttttccacg atgctcctcg tgggtggggg tccatctttg ggaccactgt cggcagaggc
     2521 atcttcaacg atggcctttc ctttatcgca atgatggcat ttgtaggagc caccttcctt
     2581 ttccactatc ttcacaataa agtgacagat agctgggcaa tggaatccga ggaggtttcc
     2641 ggatattacc ctttgttgaa aagtctcaat tgccctttgg tcttctgaga ctgtatcgat
     2701 ctggatttta gtactggatt ttggttttag gaattagaaa ttttattgat agaagtattt
     2761 tacaaataca aatacatact aagggtttct tatatgctca acacatgagc gaaaccctat
     2821 aggaacccta attcccttat ctgggaacta ctcacacatt attatggaga aactcgagct
     2881 tacttgtaca gctcgtccat gccgagagtg atcccggcgg cggtcacgaa ctccagcagg
     2941 accatgtgat cgcgcttctc gttggggtct ttgctcaggg cggactgggt gctcaggtag
     3001 tggttgtcgg gcagcagcac ggggccgtcg ccgatggggg tgttctgctg gtagtggtcg
     3061 gcgagctgca cgctgccgtc ctcgatgttg tggcggatct tgaagttcac cttgatgccg
     3121 ttcttctgct tgtcggccat gatatagacg ttgtggctgt tgtagttgta ctccagcttg
     3181 tgccccagga tgttgccgtc ctccttgaag tcgatgccct tcagctcgat gcggttcacc
     3241 agggtgtcgc cctcgaactt cacctcggcg cgggtcttgt agttgccgtc gtccttgaag
     3301 aagatggtgc gctcctggac gtagccttcg ggcatggcgg acttgaagaa gtcgtgctgc
     3361 ttcatgtggt cggggtagcg gctgaagcac tgcacgccgt aggtcagggt ggtcacgagg
     3421 gtgggccagg gcacgggcag cttgccggtg gtgcagatga acttcagggt cagcttgccg
     3481 taggtggcat cgccctcgcc ctcgccggac acgctgaact tgtggccgtt tacgtcgccg
     3541 tccagctcga ccaggatggg caccaccccg gtgaacagct cctcgccctt gctcaccatg
     3601 agacctacgg tctcggatct cattgccccc cggatctgcg aaagctcgag agagatagat
     3661 ttgtagagag agactggtga tttcagcgtg tcctctccaa atgaaatgaa cttccttata
     3721 tagaggaagg gtcttgcgaa ggatagtggg attgtgcgtc atcccttacg tcagtggaga
     3781 tatcacatca atccacttgc tttgaagacg tggttggaac gtcttctttt tccacgatgc
     3841 tcctcgtggg tgggggtcca tctttgggac cactgtcggc agaggcatct tgaacgatag
     3901 cctttccttt atcgcaatga tggcatttgt aggtgccacc ttccttttct actgtccttt
     3961 tgatgaagtg acagatagct gggcaatgga atccgaggag gtttcccgat attacccttt
     4021 gttgaaaagt ctcaatagcc ctttggtctt ctgagactgt atctttgata ttcttggagt
     4081 agacgagagt gtcgtgctcc accatgttca catcaatcca cttgctttga agacgtggtt
     4141 ggaacgtctt ctttttccac gatgctcctc gtgggtgggg gtccatcttt gggaccactg
     4201 tcggcagagg catcttgaac gatagccttt cctttatcgc aatgatggca tttgtaggtg
     4261 ccaccttcct tttctactgt ccttttgatg aagtgacaga tagctgggca atggaatccg
     4321 aggaggtttc ccgatattac cctttgttga aaagtctcaa tagccctttg gtcttctgag
     4381 actgtatctt tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc
     4441 tgctctagct cacgttgacg gaaactgcag aacgatctca gttaggtcat gggaaagctg
     4501 atattcatcc ctctgagact ctatataatt aaatgcgttt gggggattag ctaattgaga
     4561 atccatatag agaaatatgg ccgcgcagcg acgccggttg aagaatatga acaaggaata
     4621 agatgaacaa ggaagaagat gaacaagcaa taatttaatt aattattgtt aataagcata
     4681 aattatgaaa ataattttga tggtaatgaa agtattataa ataatattta ggagaaaata
     4741 ttgttaagga aatatctggt tatgtttatt tgttaaggta tatgggtttt tatagaaacc
     4801 caggagcagc tatggtaaga aacccaggac tcaaaaaatg cctctcaact tctctcatat
     4861 gttgcggagt ctggagtccc atttatacca tagaaagcat tgggggtata gcatggtaat
     4921 ttcacatcgg actccatacc gcggccatct atataatatt actgatggcc gcgcgaagtc
     4981 ccggtatgcc ttttcagccg ttggatctgg agccgtacct ttttcgcgtt cccattttgc
     5041 ccctggagtc ccgagctatt tacgaccccc tgccaccgag tccccttcgt ggagcgctct
     5101 tcgtccaaca cgtgtcccaa cttttgcgca tttttgacta cctgtcgcgc acttgaatta
     5161 aagttgccct tttattttct tccaattatt ttgcggctga gtagtctagt ggatcccccg
     5221 gagctccact tgggataggt taagaaaata tgtctggctt gtaaacgaaa agaatcgggg
     5281 tttctaggca ttttttaaat agtgacccag gactcaaaaa ctagctctca acttctctca
     5341 tatgttgtgg agtctggagt cctttatata tgtttacgct atagaaggca tttggggact
     5401 ctcccgggcc caccatagga ctccataccg cggccatcta tataatatta ctgatggccg
     5461 cgcggatttt ggagccccct tttgtcgtta gcgcgacctt taattcaaat taaaggttgc
     5521 ttttttctat cgtccgatca ttttgcgcct gactagccta gatattagca acaacttcgc
     5581 ggctaagttg ttgaccgtca agtataaatt aatgggctta tggcctaatg ggtatagagc
     5641 aaaatggcat gcctgcagac tagtgcttta ctctgatccg cagatatttc atcaggaaag
     5701 agctttgcaa gttccttcat tgtagcatca ataatttctg agtcactaca tgcaatccat
     5761 tcttctgctg gggcaaaaac taactccaac atggattggt ttggattgta atattcctta
     5821 catgttacag acatgtcagc ataaacactt aaaagcgggc ttctgctaaa gagtagatga
     5881 tcataggtgt tcttcaattt cctatcaaac cagatgtgaa cgttgataac tggaactcca
     5941 actaatttct ctaatttctt gaagtatgaa atctctctcc agtcttcagg cactagtgaa
     6001 ttcgctagcg ttaactggcc ataatcctgt gtatgcaacg ttgaaaattc ggatctattt
     6061 ttacgattcg atcatgaatt aataaaattt aaattttatt gaatgatttt ctagtacata
     6121 actgacatac gacttatcag ttgcgaaacg aacagctcta attacattgt taagtgaaat
     6181 tacgcctaat tcgtctaagt acaacataac caactgttta aacctaatta aataagtcga
     6241 cccagaagct gtcagagatg tcgtccagac ttggaagttc aggtaagctt tgtggagatg
     6301 caacgctctc cgtaagttgt ggttgaatcg gatctggacg tggtatatac gcgtcctggt
     6361 gtatagcaga tcctctactc cgtatatctt gaaatatagg ggatttatta tctcccagat
     6421 atacacgcca ttctctgcct gacgtgcagt gatgatttcc cctgtgcgtg aatccatgac
     6481 cggtgcagcc tatgtggaag tatatggtgc acccacagtt tagatcaatg cgtcgtcgtc
     6541 taatcgccct cctcttggct tgcctgtgtg ccttcttgat agaggggggc gtcgagagtg
     6601 atgaagataa cattcttgag agtccaatgt ttgagcgacg cattttccgc tttatccagg
     6661 aaatctttat agctggatcc ctcgcctgga ttgcaaagca cgattgaagg gatccctcct
     6721 ttaatttgaa ctggtttccc gtacttgcaa tttgactgcc agtctttttg ggccccaaga
     6781 agctctttcc aatgctttag ctttaggtat tgcggtgcta cgtcatcaat gacgttatag
     6841 gcaacattat tcgagtacac tcgattattg aagtccaggt gtccactgag gtaattatgt
     6901 gggcctaaag cacgagccca catcgtcttg cccgttctag aatcaccttc cactatgaga
     6961 ctaactggtc ttacaggccg cgcagcggga cccattccaa aataatcatc cgcccattct
     7021 tgcatctgtt caggaacgtt agtgaaagag gaaagtggaa atggagggac ccattgttca
     7081 ggcgcctttt tgaaaaggcg ttccaagttc gctgtaatgt tatggtaact cacgatgaac
     7141 gcccttggat ctccggcccg gataattgca agagcttccg ccaaactagg tgcattgatg
     7201 gcgttataat agacgtcgtc tttattcttc tttgtacccc caggcacttt gaactgtccg
     7261 gattcacaac aatcgccgtc tttggtgatg taattcttga cggcgttggc gtctttggct
     7321 gcttgtatgt ttgggtgaaa attggtcgac cttctggggt gagtaaggtc gaaaaatctc
     7381 ttattcttga tgttggactt tccagagagt tggatgagac agtgaaggtg tgggaatccg
     7441 tccgagtgct cctctctggc aactcgaata tatgttggtt tgacgactga ccatgataag
     7501 gtttgaagca tttgaagagc ttcatttttt gggatatcgc acttgggata ggttaagaaa
     7561 atatgtctgg cttgtaaacg aaaagaatcg gggtttctag gcatttttta aatagtgacc
     7621 caggactcaa aaactagctc tcaacttctc tcatatgttg tggagtctgg agtcctttat
     7681 atatgtttac gctatagaag gcatttgggg actctcccgg gcccaccata ggactccata
     7741 ccgcggccat ctatataata ttactgatgg ccgcgcggat tttggagccc ccttttgtcg
     7801 ttagcgcgac ctttaattca aattaaaggt tgcttttttc tatcgtccaa tcattttgcg
     7861 cctgactagc ctagatatta gcaacaactt cgcgactaag ttgttgaccg tcaagtataa
     7921 attaatgggc ttatggccca atgggtatag agcaaaatgc ccaagcgtga tgccccgtgg
     7981 cgcttattag cgggtacgtc taaggtaagt cgaaatgcca actactctcc cttgagagta
     8041 ttgggcccta aagttaataa gggacctcga gggggggccc ggtacccggg gatcctctag
     8101 agtcgacctg caggcatgca agcttggcac tggccgtcgt tttacaacgt cgtgactggg
     8161 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
     8221 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
     8281 aatgctagag cagcttgagc ttggatcaga ttgtcgtttc ccgccttcag tttaaactat
     8341 cagtgtttga caggatatat tggcgggtaa acctaagaga aaagagcgtt tattagaata
     8401 atcggatatt taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc
     8461 aaccacaggg ttcccctcgg gatcaaagta ctttgatcca acccctccgc tgctatagtg
     8521 cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa cgacatgtcg cacaagtcct
     8581 aagttacgcg acaggctgcc gccctgccct tttcctggcg ttttcttgtc gcgtgtttta
     8641 gtcgcataaa gtagaatact tgcgactaga accggagaca ttacgccatg aacaagagcg
     8701 ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga cgaccaggac ttgaccaacc
     8761 aacgggccga actgcacgcg gccggctgca ccaagctgtt ttccgagaag atcaccggca
     8821 ccaggcgcga ccgcccggag ctggccagga tgcttgacca cctacgccct ggcgacgttg
     8881 tgacagtgac caggctagac cgcctggccc gcagcacccg cgacctactg gacattgccg
     8941 agcgcatcca ggaggccggc gcgggcctgc gtagcctggc agagccgtgg gccgacacca
     9001 ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg cattgccgag ttcgagcgtt
     9061 ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc caaggcccga ggcgtgaagt
     9121 ttggcccccg ccctaccctc accccggcac agatcgcgca cgcccgcgag ctgatcgacc
     9181 aggaaggccg caccgtgaaa gaggcggctg cactgcttgg cgtgcatcgc tcgaccctgt
     9241 accgcgcact tgagcgcagc gaggaagtga cgcccaccga ggccaggcgg cgcggtgcct
     9301 tccgtgagga cgcattgacc gaggccgacg ccctggcggc cgccgagaat gaacgccaag
     9361 aggaacaagc atgaaaccgc accaggacgg ccaggacgaa ccgtttttca ttaccgaaga
     9421 gatcgaggcg gagatgatcg cggccgggta cgtgttcgag ccgcccgcgc acgtctcaac
     9481 cgtgcggctg catgaaatcc tggccggttt gtctgatgcc aagctggcgg cctggccggc
     9541 cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa aggtgatgtg tatttgagta
     9601 aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga tgagtaaata aacaaatacg
     9661 caaggggaac gcatgaaggt tatcgctgta cttaaccaga aaggcgggtc aggcaagacg
     9721 accatcgcaa cccatctagc ccgcgccctg caactcgccg gggccgatgt tctgttagtc
     9781 gattccgatc cccagggcag tgcccgcgat tgggcggccg tgcgggaaga tcaaccgcta
     9841 accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg tgaaggccat cggccggcgc
     9901 gacttcgtag tgatcgacgg agcgccccag gcggcggact tggctgtgtc cgcgatcaag
     9961 gcagccgact tcgtgctgat tccggtgcag ccaagccctt acgacatatg ggccaccgcc
    10021 gacctggtgg agctggttaa gcagcgcatt gaggtcacgg atggaaggct acaagcggcc
    10081 tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg gtgaggttgc cgaggcgctg
    10141 gccgggtacg agctgcccat tcttgagtcc cgtatcacgc agcgcgtgag ctacccaggc
    10201 actgccgccg ccggcacaac cgttcttgaa tcagaacccg agggcgacgc tgcccgcgag
    10261 gtccaggcgc tggccgctga aattaaatca aaactcattt gagttaatga ggtaaagaga
    10321 aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc gagcgcacgc agcagcaagg
    10381 ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa gcgggtcaac tttcagttgc
    10441 cggcggagga tcacaccaag ctgaagatgt acgcggtacg ccaaggcaag accattaccg
    10501 agctgctatc tgaatacatc gcgcagctac cagagtaaat gagcaaatga ataaatgagt
    10561 agatgaattt tagcggctaa aggaggcggc atggaaaatc aagaacaacc aggcaccgac
    10621 gccgtggaat gccccatgtg tggaggaacg ggcggttggc caggcgtaag cggctgggtt
    10681 gcctgccggc cctgcaatgg cactggaacc cccaagcccg aggaatcggc gtgagcggtc
    10741 gcaaaccatc cggcccggta caaatcggcg cggcgctggg tgatgacctg gtggagaagt
    10801 tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc agaagcacgc cccggtgaat
    10861 cgtggcaagc ggccgctgat cgaatccgca aagaatcccg gcaaccgccg gcagccggtg
    10921 cgccgtcgat taggaagccg cccaagggcg acgagcaacc agattttttc gttccgatgc
    10981 tctatgacgt gggcacccgc gatagtcgca gcatcatgga cgtggccgtt ttccgtctgt
    11041 cgaagcgtga ccgacgagct ggcgaggtga tccgctacga gcttccagac gggcacgtag
    11101 aggtttccgc agggccggcc ggcatggcca gtgtgtggga ttacgacctg gtactgatgg
    11161 cggtttccca tctaaccgaa tccatgaacc gataccggga agggaaggga gacaagcccg
    11221 gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt ctgccggcga gccgatggcg
    11281 gaaagcagaa agacgacctg gtagaaacct gcattcggtt aaacaccacg cacgttgcca
    11341 tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac ggtatccgag ggtgaagcct
    11401 tgattagccg ctacaagatc gtaaagagcg aaaccgggcg gccggagtac atcgagatcg
    11461 agctagctga ttggatgtac cgcgagatca cagaaggcaa gaacccggac gtgctgacgg
    11521 ttcaccccga ttactttttg atcgatcccg gcatcggccg ttttctctac cgcctggcac
    11581 gccgcgccgc aggcaaggca gaagccagat ggttgttcaa gacgatctac gaacgcagtg
    11641 gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg caagctgatc gggtcaaatg
    11701 acctgccgga gtacgatttg aaggaggagg cggggcaggc tggcccgatc ctagtcatgc
    11761 gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc ctaatgtacg gagcagatgc
    11821 tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg tgtctttcct gtggatagca
    11881 cgtacattgg gaacccaaag ccgtacattg ggaaccggaa cccgtacatt gggaacccaa
    11941 agccgtacat tgggaaccgg tcacacatgt aagtgactga tataaaagag aaaaaaggcg
    12001 atttttccgc ctaaaactct ttaaaactta ttaaaactct taaaacccgc ctggcctgtg
    12061 cataactgtc tggccagcgc acagccgaag agctgcaaaa agcgcctacc cttcggtcgc
    12121 tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc ggccgctggc cgctcaaaaa
    12181 tggctggcct acggccaggc aatctaccag ggcgcggaca agccgcgccg tcgccactcg
    12241 accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt tcggtgatga cggtgaaaac
    12301 ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
    12361 agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
    12421 cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg
    12481 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
    12541 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
    12601 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
    12661 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
    12721 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
    12781 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
    12841 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
    12901 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
    12961 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
    13021 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
    13081 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
    13141 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
    13201 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
    13261 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
    13321 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
    13381 aagggatttt ggtcatgcat tctaggtact aaaacaattc atccagtaaa atataatatt
    13441 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
    13501 cttccccgat atcctccctg atcgaccgga cgcagaaggc aatgtcatac cacttgtccg
    13561 ccctgccgct tctcccaaga tcaataaagc cacttacttt gccatctttc acaaagatgt
    13621 tgctgtctcc caggtcgccg tgggaaaaga caagttcctc ttcgggcttt tccgtcttta
    13681 aaaaatcata cagctcgcgc ggatctttaa atggagtgtc ttcttcccag ttttcgcaat
    13741 ccacatcggc cagatcgtta ttcagtaagt aatccaattc ggctaagcgg ctgtctaagc
    13801 tattcgtata gggacaatcc gatatgtcga tggagtgaaa gagcctgatg cactccgcat
    13861 acagctcgat aatcttttca gggctttgtt catcttcata ctcttccgag caaaggacgc
    13921 catcggcctc actcatgagc agattgctcc agccatcatg ccgttcaaag tgcaggacct
    13981 ttggaacagg cagctttcct tccagccata gcatcatgtc cttttcccgt tccacatcat
    14041 aggtggtccc tttataccgg ctgtccgtca tttttaaata taggttttca ttttctccca
    14101 ccagcttata taccttagca ggagacattc cttccgtatc ttttacgcag cggtattttt
    14161 cgatcagttt tttcaattcc ggtgatattc tcattttagc catttattat ttccttcctc
    14221 ttttctacag tatttaaaga taccccaaga agctaattat aacaagacga actccaattc
    14281 actgttcctt gcattctaaa accttaaata ccagaaaaca gctttttcaa agttgttttc
    14341 aaagttggcg tataacatag tatcgacgga gccgattttg aaaccgcggt gatcacaggc
    14401 agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc
    14461 aaacccggca gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc
    14521 cgccttacaa cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt
    14581 ggtgattttg tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat
    14641 tgtggtgtaa acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg
    14701 tactgaatta acgccgaatt aattcgggga tagccctttg gtcttctgag actgtatctt
    14761 tgatattctt ggagtagacg agagtgtcgt gctccaccat gttggcaagc tgctctagcc
    14821 aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag
    14881 gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca
    14941 ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag
    15001 cggataacaa tttcacacag gaaacagcta tgacatgatt acg
//
