LOCUS       sequence-487003-        5212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Bacterial expression of green glucose sensor with inert 
            mIRFP670nano3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5212)
  AUTHORS   Marvin JS, Machler P, Meng C, Ates T, Patel RH, Adhikari R, Makurath
            MA, Ku Z, Feliciano D, Atasoy D, Cui G, Kleinfeld D, Brown TA
  TITLE     iGlucoSnFR2: A genetically encoded fluorescent sensor for measuring 
            intracellular or extracellular glucose in vivo in mouse brain.
  JOURNAL   Sci Adv. 2025 Nov 14;11(46):eadz3889. doi: 10.1126/sciadv.adz3889. 
            Epub 2025 Nov 12.
  PUBMED    41223288
REFERENCE   2  (bases 1 to 5212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-11-14"; volume: "11(46):eadz3889. doi"; pages: " 
            10.1126/sciadv.adz3889. Epub 2025 Nov 12"
COMMENT     Sequence Label: pNoRBP.iGlucoSnFR2.mIRFP670nano3
FEATURES             Location/Qualifiers
     source          1..5212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             70..92
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     1296..1317
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1420..1441)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2530..2547
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(2613..2631)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      2627..2674
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2771..3226
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2858..2877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3068..3089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3252..3356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3357..3425,3426..4217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3575..3594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4388..4976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4877..4896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5130..5147
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
       61 agaaataatt ttgtttaact ttaagaagga gatatacata tgagatctaa gctggagatt
      121 ttctcttggt gggctgggga tgaaggccct gccctggagg cactcatcag actgtataaa
      181 cagaagtatc ctggagtaga ggtgataaac gccactgtca ccggaggtgc cggtgtaaat
      241 gctcgcgctg tccttaagac aaggatgctt ggtggtgatc ctcctgatac atttcaagtt
      301 gcagcaggga tggagttgat aggcacatgg gtggtggcta acagaatgga ggatctcagc
      361 gccttgtttc gacaagaggg ttggcttcaa gccttcccca agggccttat tgatctgatt
      421 agttataaag gtggtatatg gagtgtgccc gttaatattc acaggtcaaa cgtaatgtgg
      481 tatttgcctg caaagctaaa agagtgggga gttaaccccc cacggacttg ggatgagttt
      541 cttgccacct gtcaaacact taaacaaaag ggtctggaag ctccactggc tctcggggaa
      601 aattggacac aacaacacct ctgggagagt gtcgccttgg ctgtattggg gcctgatgat
      661 tggaataatc tgtggaatgg gaagctgaag ttcacagacc ctaaagcagt tagggcatgg
      721 gaagtctttg gccgcgtttt ggactgtgcc aacaaggatg cagcaggctt gtcttggcag
      781 caagctgtcg atcgggtagt acaggggaaa gccgctttta acgtaatggg cgactgggca
      841 gcaggatata tgactaccac tctcaagttg aagcctggta cagatttcgc atgggcacca
      901 agtcccggga cacagggcgt ctttatgatg gtttccgact ctttcggact tcccaaagga
      961 gccaaaaatc gacaaaacgc tatcaactgg cttcgattgg tcggctctaa agaaggtcaa
     1021 gatacattca acccactgaa agggtccatc gctgctcggc tcgatagcga cccaagcaag
     1081 tatccgtgta acgtctatat caccgccgac aagcagaaga acggcatcaa ggcgaacttc
     1141 aagatccgcc acaacgtgga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     1201 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctggt gacccagtcc
     1261 gtgctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     1321 gccgccggga tcactctcgg catggacgag ctgtacaagg gcggtaccgg agggagcatg
     1381 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     1441 gtaaacggcc acaagttcag cgtgcgcggc gagggcgagg gcgatgccac caacggcaag
     1501 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     1561 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     1621 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cagcttcaag
     1681 gacgacggca cctacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     1741 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     1801 gagtacaact ttaacaaccc caacgcatac ggacagagtg caatgcgaga ttggcggagt
     1861 aatcgcatcg ttggctccct ggtagcagga gcagtggccc ctgaaagctc catgtcacaa
     1921 ttcggaacag taatggaaat tttcctgcaa acacgcaacc cacaagcagc agcaaatgca
     1981 gcacaagcta ttgctgacca agttggcctg ggtcgccttg gtcagctgca gtctagaggc
     2041 tccggaggtg gaggtactgg aggaggtggg tcaggtggag gtggatcaat ggcaaacctg
     2101 gacaagatgc tgaacaccac cgtgaccgag gtgcgcaagt tcctgcaagc ggacagagtg
     2161 tgcgtgttca agttcgagga agattactcc ggcaccgtct cgcacgaagc cgtggacgac
     2221 agatggatta gcatcctgaa gacccaggtg caggacagat acttcatgga aaccagaggc
     2281 gaggaatacg tccacggcag ataccaggcc atcgccgaca tctacacagc caatctggtc
     2341 gagtgctaca gagacctgct gatcgagttt caggtgcggg ccattctggc tgtccccatc
     2401 ctgcaaggca agaagctgtg gggcctgctg gtggcccacc aactggccgg ccctcgggag
     2461 tggcagacct gggaaatcga cttcctgaaa cagcaagccg tggtgatggg catcgccatc
     2521 cagcagagcc atcaccatca tcaccattaa gaattcgaag cttgatccgg ctgctaacaa
     2581 agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag cataacccct
     2641 tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta tatccggatc
     2701 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     2761 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     2821 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     2881 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     2941 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3001 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3061 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3121 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3181 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     3241 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     3301 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     3361 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     3421 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     3481 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     3541 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     3601 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     3661 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     3721 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     3781 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     3841 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     3901 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     3961 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4021 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4081 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4141 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4201 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     4261 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     4321 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     4381 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     4441 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     4501 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     4561 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     4621 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     4681 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     4741 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     4801 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     4861 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     4921 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     4981 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5041 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5101 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5161 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc ag
//
