LOCUS       sequence-487008-       30004 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob 
            - spike base (Pvc8) loaded with Cas9 on CTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 30004)
  AUTHORS   Kreitz J, Yang V, Friedrich MJ, Pham J, Macrae RK, Zhang F
  TITLE     Targeted delivery of diverse biomolecules with engineered bacterial 
            nanosyringes.
  JOURNAL   Nat Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x.
  PUBMED    40796978
REFERENCE   2  (bases 1 to 30004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x."
COMMENT     Sequence Label: pAWP78-PVCpnf_pvc13-Ad5_pvc8-Cas9
FEATURES             Location/Qualifiers
     source          1..30004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(338..1153)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(338..1153)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1061..1080
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1145..1164
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1457..2605)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEGIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2877..3248)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQEELGKVMMGVVRPRAEP"
     oriT            3281..3390
                     /note="incP origin of transfer"
     rep_origin      3543..4174
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4483..5070
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4971..4990
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             12857..12877
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             12902..17005
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             12902..17005
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagatgatga taagaatggt cagtcaccag ataaaatgat ctctggtcaa ttttaggaaa
       61 gctgaaaaaa cctgaccaaa tgcgtcgtaa attctatgct tagaagactc ggtgccagtg
      121 taatacctaa gatatggttt ttatttccat atctgttcga aaacttagaa ataacaagtt
      181 caacaataac cagggacttt aaatatcgga tccttgtcgg gaagatgcgt gatctgatcc
      241 ttcaactcag caaaagttcg atttattcaa caaagccgcc gtcccgtcaa gtcagcgtaa
      301 tgctctgcca gtgttacaac caattaacca attctgatta gaaaaactca tcgagcatca
      361 aatgaaactg caatttattc atatcaggat tatcaatacc atatttttga aaaagccgtt
      421 tctgtaatga aggagaaaac tcaccgaggc agttccatag gatggcaaga tcctggtatc
      481 ggtctgcgat tccgactcgt ccaacatcaa tacaacctat taatttcccc tcgtcaaaaa
      541 taaggttatc aagtgagaaa tcaccatgag tgacgactga atccggtgag aatggcaaaa
      601 gcttatgcat ttctttccag acttgttcaa caggccagcc attacgctcg tcatcaaaat
      661 cactcgcatc aaccaaaccg ttattcattc gtgattgcgc ctgagcgaga cgaaatacgc
      721 gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg caaccggcgc aggaacactg
      781 ccagcgcatc aacaatattt tcacctgaat caggatattc ttctaatacc tggaatgctg
      841 ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc aggagtacgg ataaaatgct
      901 tgatggtcgg aagaggcata aattccgtca gccagtttag tctgaccatc tcatctgtaa
      961 catcattggc aacgctacct ttgccatgtt tcagaaacaa ctctggcgca tcgggcttcc
     1021 catacaatcg atagattgtc gcacctgatt gcccgacatt atcgcgagcc catttatacc
     1081 catataaatc agcatccatg ttggaattta atcgcggcct cgagcaagac gtttcccgtt
     1141 gaatatggct cataacaccc cttgtattac tgtttatgta agcagacagt tttattgttc
     1201 atgatgatat atttttatct tgtgcaatgt aacatcagag attttgagac acaacgtggc
     1261 tttcgacttc cggcaagcta tacgcgccct agaattgtca attttaatcc tctgtttatc
     1321 ggcagttcgt agagcgcgcc gtgcgtcccg agcgatactg agcgaagcaa gtgcgtcgag
     1381 cagtgcccgc ttgttcctga aatgccagta aagcgctggc tgctgaaccc ccagccggaa
     1441 ctgaccccac aaggccctag cgtttgcaat gcaccaggtc atcattgacc caggcgtgtt
     1501 ccaccaggcc gctgcctcgc aactcttcgc aggcttcgcc gacctgctcg cgccacttct
     1561 tcacgcgggt ggaatccgat ccgcacatga ggcggaaggt ttccagcttg agcgggtacg
     1621 gctcccggtg cgagctgaaa tagtcgaaca tccgtcgggc cgtcggcgac agcttgcggt
     1681 acttctccca tatgaatttc gtgtagtggt cgccagcaaa cagcacgacg attccctcgt
     1741 cgatcaggac ctggcaacgg gacgttttct tgccacggtc caggacgcgg aagcggtgca
     1801 gcagcgacac cgattccagg tgcccaacgc ggtcggacgt gaagcccatc gccgtcgcct
     1861 gtaggcgcga caggcattcc tcggccttcg tgtaataccg gccattgatc gaccagccca
     1921 ggtcctggca aagctcgtag aacgtgaagg tgatcggctc gccgataggg gtgcgcttcg
     1981 cgtactccaa cacctgctgc cacaccagtt cgtcatcgtc ggcccgcagc tcgacgccgg
     2041 tgtaggtgat cttcacgtcc ttgttgacgt ggaaaatgac cttgttttgc agcgcctcgc
     2101 gcgggatttt cttgttgcgc gtggtgaaca gggcagagcg ggccgtgtcg tttggcatcg
     2161 ctcgcatcgt gtccggccac ggcgcaatat cgaacaagga aagctgcatt tccttgatct
     2221 gctgcttcgt gtgtttcagc aacgcggcct gcttggcctc gctgacctgt tttgccaggt
     2281 cctcgccggc ggtttttcgc ttcttggtcg tcatagttcc tcgcgtgtcg atggtcatcg
     2341 acttcgccaa acctgccgcc tcctgttcga gacgacgcga acgctccacg gcggccgatg
     2401 gcgcgggcag ggcaggggga gccagttgca cgctgtcgcg ctcgatcttg gccgtagctt
     2461 gctggaccat cgagccgacg gactggaagg tttcgcgggg cgcacgcatg acggtgcggc
     2521 ttgcgatggt ttcggcatcc tcggcggaaa accccgcgtc gatcagttct tgcctgtatg
     2581 ccttccggtc aaacgtccga ttcattcacc ctccttgcgg gattgccccg actcacgccg
     2641 gggcaatgtg cccttattcc tgatttgacc cgcctggtgc cttggtgtcc agataatcca
     2701 ccttatcggc aatgaagtcg gtcccgtaga ccgtctggcc gtccttctcg tacttggtat
     2761 tccgaatctt gccctgcacg aataccagct ccgcgaagtc gctcttcttg atggagcgca
     2821 tggggacgtg cttggcaatc acgcgcaccc cccggccgtt ttagcggcta aaaaagtcat
     2881 ggctctgccc tcgggcggac cacgcccatc atgaccttgc caagctcttc ctgcttctct
     2941 tcgatcttcg ccagcagggc gaggatcgtg gcatcaccga accgcgccgt gcgcgggtcg
     3001 tcggtgagcc agagtttcag caggccgccc aggcggccca ggtcgccatt gatgcgggcc
     3061 agctcgcgga cgtgctcata gtccacgacg cccgtgattt tgtagccctg gccgacggcc
     3121 agcaggtagg cctacaggct catgccggcc gccgccgcct tttcctcaat cgctcttcgt
     3181 tcgtctggaa ggcagtacac cttgataggt gggctgccct tcctggttgg cttggtttca
     3241 tcagccatcc gcttgccctc atctgttacg ccggcggtag ccggccagcc tcgcagagca
     3301 ggattcccgt tgagcaccgc caggtgcgaa taagggacag tgaagaagga acacccgctc
     3361 gcgggtgggc ctacttcacc tatcctgccc ggctgacgcc gttggataca ccaaggaaag
     3421 tctacacgaa ccctttggca aaatcctgta tatcgtgcga aaaaggatgg atataccgaa
     3481 aaaatcgcta taatgacccc gaagcagggt tatgcagcgg aaaagatccg tcgacccttt
     3541 ccgacgctca ccgggctggt tgccctcgcc gctgggctgg cggccgtcta tggccctgca
     3601 aacgcgccag aaacgccgtc gaagccgtgt gcgagacacc gcggccgccg gcgttgtgga
     3661 tacctcgcgg aaaacttggc cctcactgac agatgagggg cggacgttga cacttgaggg
     3721 gccgactcac ccggcgcggc gttgacagat gaggggcagg ctcgatttcg gccggcgacg
     3781 tggagctggc cagcctcgca aatcggcgaa aacgcctgat tttacgcgag tttcccacag
     3841 atgatgtgga caagcctggg gataagtgcc ctgcggtatt gacacttgag gggcgcgact
     3901 actgacagat gaggggcgcg atccttgaca cttgaggggc agagtgctga cagatgaggg
     3961 gcgcacctat tgacatttga ggggctgtcc acaggcagaa aatccagcat ttgcaagggt
     4021 ttccgcccgt ttttcggcca ccgctaacct gtcttttaac ctgcttttaa accaatattt
     4081 ataaaccttg tttttaacca gggctgcgcc ctgtgcgcgt gaccgcgcac gccgaagggg
     4141 ggtgcccccc cttctcgaac cctcccggcc cgctaacgcg ggcctcccat ccccccaggg
     4201 gctgcgcccc tcggccgcga acggcctcac cccaaaaatg gcagccaagc tgacccgcta
     4261 gggacgtgaa gtcgattcct tcgatggtta gcaatcaaag actcagagtg ctgtctggag
     4321 cgtgaatcta acggtacgta tctcgattgc tcggtcgcta ttcgcactct gcgaaagttc
     4381 gtaccgctca ttcactaggt tgcgaatcat gaccaaaatc ccttaacgtg agttttcgtt
     4441 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct
     4501 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc
     4561 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc
     4621 aaatactgtt cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc
     4681 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc
     4741 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg
     4801 aacgggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac
     4861 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat
     4921 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc
     4981 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga
     5041 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc
     5101 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg
     5161 gataaccgta ttaccgcctt tgagtgagct gtcgcgaatg catctagata taattacatc
     5221 ttcatcattc ggtattttaa atgacatata tcctccttcc gaatagtttt aatggtgatg
     5281 ttcgattgga cagtgttttc cattattaaa attttaagta tcacatcatg tttaatgaag
     5341 gattgatgtt tattagaata ttcataatag ctttgtcttt gcctctatgt aaatgaaatt
     5401 gcataaaaag caaattgcat tattggcaat gtacttattc tggatttttc ggcacgataa
     5461 attattggtg taagttttat atcatgagta aaaattcgga atgatattcc tgtcattttg
     5521 tggcggtagc gttactcaag atattatcct acatcatatg ttgattattg taatcaatcg
     5581 agctattttt tgtctaaaaa ggggctttta tgtctacaag tacatctcaa attgcggttg
     5641 aatatcctat tcctgtctat cgctttattg tttctgtcgg agatgagaaa attccattta
     5701 atagtgtttc aggattagat attagttatg acaccattga ataccgagat ggtgttggta
     5761 attggttcaa aatgccgggt cagagtcaga gcactaatat caccttgcgt aaaggcgttt
     5821 tcccggggaa aacagaactg tttgattgga ttaactctat tcagcttaat caggtagaga
     5881 aaaaggatat taccatcagt ttaactaatg atgcaggtac cgaattatta atgacctgga
     5941 atgtttctaa tgcttttccc acttcattga cttcaccttc atttgatgcc accagtaatg
     6001 atattgcagt acaggaaatt acgctgatgg cagatcgggt gattatgcag gctgtttgaa
     6061 gcattgatat ttaatcatct catataaggg aacttttatg acaaccgtta ccagttatcc
     6121 tggcgtttat attgaagaat taaatagcct ggccttgtca gtttcaaata gcgccacagc
     6181 ggttcctgtt tttgctgtgg acgaacaaaa ccaatatatt agtgaagata atgcaatccg
     6241 tattaattcg tggatggatt atcttaatct gattggcaat tttaataatg aagacaaatt
     6301 agatgtttct gtgcgtgctt attttgccaa tggaggtgga tattgttatc tcgtcaaaac
     6361 aacgagttta gaaaaaatta ttccaacctt ggatgatgta accttattgg ttgctgcggg
     6421 cgaagatatt aaaacgacag tagatgtttt atgtcagcca ggaaaagggt tattcgcagt
     6481 ctttgatggc cctgaaacag agttgactat caacggtgcg gaagaggcaa aacaagccta
     6541 taccgccaca ccattcgctg cggtttatta tccttggttg aaagcggatt gggctaacat
     6601 agatattcca cccagtgcag tgatggcggg agtttatgca tcggtggatt tatcccgtgg
     6661 tgtatggaaa gcgcctgcca atgttgcgtt gaaagggggc ctggaaccta aatttttagt
     6721 cacggatgaa ttgcagggtg aatataacac tggccgcgct atcaatatga ttcgtaattt
     6781 cagtaacaca ggtactacgg tttggggtgc aagaaccctg gaagataaag acaattggcg
     6841 ttatgttcca gtgcgacgct tgtttaattc tgtggagcgg gatatcaagc gtgccatgag
     6901 ctttgctatg ttcgagccta ataatcagcc tacttgggag cgggtacggg cggcgattag
     6961 caactacctt tatagcctgt ggcaacaggg gggattagct ggcagcaaag aagaagacgc
     7021 ttattttgtg caaattggta aaggtataac gatgacacag gagcagattg atgcagggca
     7081 aatgattgtt aaagtcggtt tggctgctgt acggcctgcg gaatttatca ttctccagtt
     7141 tacgcaagat gtagaacagc gttaatcata tgattatgag gagttatcat gtctgctatt
     7201 ctgaaagcgc ctggcgttta tattgaagaa gacgcttccc tagcgttgtc tgtcagtaac
     7261 agcgcgactg ccgtgcctgt ttttatcgga aaatttactc cgacagtggt tgattcaatc
     7321 caagtctgta cccgtatcag caactggctt gaattcactt cctctttttc cctagctcca
     7381 acagtcgaga ttgttgtcca atctaacact gaatctgaat ctgaatctga aacttaccac
     7441 tatattgaga caatcaattt atctccagct gtggaagcat tgcgactcta ttttcaaaat
     7501 ggcggaggag cttgctatat ctacccatta aatgatgctg aagatgaatt ggttctggcg
     7561 gccataccag aagtcattga acagaaaggt gatattactc tgttggtttg cccggaactc
     7621 gatctggatt acaaaactaa gatctatggc gcagtgagct cactgttgaa tgataacaaa
     7681 gtgggctatt tcctgattgc ggatagcaat gatggagaat ctgtgtcagg agtatggaat
     7741 agtgctaagg ccgccgccta ttatccccag ttggaaacta acctaaaatt ttccacgttg
     7801 cctggggata aggacattcg tatcagcggt tatcaggatg atgatgaaac acataaaccg
     7861 aaaaacttgg atgagctcag gacaatcaac gaggcgttgg cacaggatat tgatgcaaga
     7921 ttgctcgagg agaaacaacg tgctgtcatc attccgccaa gtgctgccat tgcgggcatt
     7981 tattgccaaa cggataatcg tcgcggtgtt tggaaagcgc cagccaacgt tgcgctcaca
     8041 gggatcggga gtttgcttga taaggtagac gatgaacggc agggagagat gaatgacaag
     8101 ggaatcaatg tcatccgttc atttaccgac cgtggtttta tggtctgggg agcccgtact
     8161 tgtgtggacg ctgccaacat cagctggcgt tatattcctg ttcgtcgcct gttcaattcc
     8221 gttgaacgag atatccgcca ggcgctgcgc gctgtgttgt ttgaaactaa tagtcagcct
     8281 acctgggtac gtgctaaggc tgccgttgat caatatcttt ataccctttg gcagaaaaat
     8341 gcattgatgg gtgctcgccc ggaagaagct tattttgtgc aaattggtca ggatatcacc
     8401 atgtccgagg ctgatattaa acagggtaag atgatcatga ctgttggttt ggcagcagtg
     8461 cggccagctg agttcatcat tctgcaattt acgcaggatg ttgttcagta atctccatga
     8521 ctaaacgcca ggcactgtat tgacagtgcc tactctaacc atcttggagg aggtgatgat
     8581 gatggagaga ctccaaccgg gtgtgacttt aacagaaagt ataatcacga tgggtcagca
     8641 agagataccc agtgctgtgc cggtgtttat tggttacacc gttcgttatc cggaacaatc
     8701 ggaagcatca gtccgtatcg acagtttggc cgagtatacc agcctgtttg gtgacgacca
     8761 tgtgatgatg tttgctgtca ggcactattt tgataatggc gggcaacagg catttgtttt
     8821 acccctgaag gacaatatgc catcagtgga gatgaccaca gctgaagcgg aaaatctgat
     8881 agccgcattg cgctctgcta cggttagcga agccattggt gggcatagtc agattacact
     8941 gattttggta ccggatatgg ctcggcttaa tgacagtgat attgatgact cctcaaccca
     9001 ggtaagcctg tggtcccaag gctgggaggc gctgctgcaa ttgagtcagg ttaggcccaa
     9061 cctctttgtg ctgttagatg cgccggataa tgttgaacag gcgcagaagt gtatgacaac
     9121 gctatcgtca gattatcgtc aatggggggc agcatattgg cctcgtctgg aaactaccta
     9181 tcagaaagaa atatctggca aggacaatga atctcaggga attttccagg ggactgttct
     9241 atcacccaca gccgcggtcg cagcggtaat tcaacgcacg gataacgacg cgggtgtttg
     9301 gaaagcaccg gccaatattg ccttatccca ggttattcga cctgttaaat cttatcttca
     9361 gggaagtgta ctgtttaaca gcagcggcac ttcgctcaat gtgatccgca gtttcccagg
     9421 taagggcata cgggtatggg gatgccgcac tctggaaaac acggataata cgcagtggcg
     9481 ctatctgcaa acacgtcggc tggtttccta tgtaacagcg catttgaccc aattggctcg
     9541 catgtatgtc ttcgagccaa ataatgaact tacctggatg aagttaaaag gacaaagtta
     9601 caactggtta cggcaattat ggttgcaggg tggcttgtat ggttcacagg aggatgaggc
     9661 atttaacatt ctgttaggcg taaacgagac gatgactgag gatgatgttc gtgcaggaaa
     9721 aatgatcatg aaagttgagt tggctgtgtt gtttcctgcc gaatttattg agatcagttt
     9781 ggtgtttaat acccaaacag aggcgctgtc ttaagaagga aaaagtacga tgaacgatta
     9841 ttacacaccc gtggtatccc atcgttttat ggcgagtttt atttttaacc gcattcccga
     9901 tccgctggat attcgttttc agcgtatctc tggccttagt cgggaactac aggtgactca
     9961 gtacagtgag ggaggagaaa atgcccgtaa taactattta gctgagaaaa tccaacacgg
    10021 tacgttgact ttggaacggg gcgtgatgac agtctcgcca ttgacctgga tgtttgatcg
    10081 ggtattgagt ggtgaaaaaa tcgcttatgc cgatgtggtg gtgatgctac tgaatgaaaa
    10141 ttcactgcca ttgtccagtt ggacgttgag caatgcgctg ccggtacgct ggcaaaccag
    10201 cgactttgac gctaacagca atgccatatt ggtgaatacc cttgaattgc gttaccagga
    10261 tatgcgctgg cttggagtca aaatatgaca gtagaaatca gagagttact tatccaggca
    10321 aaggtagtgc catcaacacg accgactgaa tcagaacggc aaaaccattc tttgatacag
    10381 gaaagtctgg atgaggcgac ttgggtggaa acgataaaac gcgaagtgtt ggccgcatta
    10441 cgcgatgagg aagggtggcg tccatgagtc tgattgaacg tggtttagct aagctgacaa
    10501 ttaatgctta taaggatagg gaagggaaga tacgggcagg aacgttgcag gccatgtata
    10561 accctgactc cttgcaactg gattaccaaa cggattatca gcaatcccaa gcgattaata
    10621 gcgaaaagca aagtagcatt tatgtacagg ccaagcccgc agggttatca cttgaattaa
    10681 tttttgatgc cacgatgccg ggtaacaaaa cccccattga agagcagctc atgcagctca
    10741 agcaactgtg cagtgtggat gcaaccagta acgagacgcg attcctgcaa gttaaatggg
    10801 gcaaaatgcg ttgggaaagt cggggttact ttgctggcag ggccaagagt ttgtctgtga
    10861 attacacttt gtttgatcgt gatgcgactc ccttgagggt acgggtaata ttggcattag
    10921 tggctgatga aagtctggtg ttgcaggaga ctgaacaaaa tctgcaatct ccggcaaaaa
    10981 tcgcattacg catacaggat ggggtatctc tggctctgat ggcagccagt acggcatcaa
    11041 cattgtcagg cggtgtggat tatctgacgc tggcctggca aaacggtctg gataatctca
    11101 atgggttcgt tccgggtgaa atattgcagg ccaccagggg agacgaatca tgagccacca
    11161 actgaaaatt attgcagatg gtaaggcact gtcacttttg gccgcggtag atgtggacac
    11221 ctgttatcgg gttaacagta taccttctgc gacattgaaa ctgagcgtac cggataggcc
    11281 actctcttct ttcagtcaga cggatgttca gacagaactg gcccactgtc aggtagggaa
    11341 aaccctgcgt ctggaattga ttgatggtag caaaaaatgg gtgctgttta atggtcttat
    11401 tacccgtaag gctctgagaa ttaagaataa gcaattattg ctcactctgg ttgtcaagca
    11461 tcggttgcaa ctgatggtgg atacccagca ttcacagctg tttaaagaca aaagcgaaaa
    11521 agcgatctta agcacgctat tgaatcagac cggaatcaat gctcgcttcg gaaagatagc
    11581 ggcgttagat caaaagcatg aacagatggt gcaatttcgt tgttcagact ggcattttct
    11641 gttgtgccga ctgtcggcaa ccggtgcatg gttgttacct gccatagaag acgttcagtt
    11701 tgttcaacct gatgctctga aatcaaactc agcctatacc ttgaagagca ggggggatga
    11761 gaacaaagac atcgttgtca aggatgctta ctggcagttt gacaatcaaa tcaaccccgc
    11821 tttgctggaa gtcagtggct gggatatcag taagcagcag gtacaatcag gcggtcgcta
    11881 cggaaaaatc gcgttgggta aggcggcact ctctcctgat ggattggcat cccttaataa
    11941 aacgggttgg gacatttgtt atagcagtcc gttaacaacc caggaaagcg gttatctggc
    12001 acagggatta ttgcttaacc agcgcatttc tggggtgaca ggagaatttt tgctcaaagg
    12061 agatgggcgt taccagttgg gagacaacat tcagctgact ggatttggtt cacagttaga
    12121 tggtacggca agcattactg aggttcgcca ccgtcttaat cggcgaattg attgggaaac
    12181 cacggtgagc attggtttac aacatgaata tttgccgata ttacctgatg ctcccgaact
    12241 acatattgcg acagtagcga aatatcagca ggacagtgcg gtgttaaacc gtatccccat
    12301 tattctgccg gtactgaatc gtcccaatga atttttgtgg gccagattgg ggaaacctta
    12361 tgctagccat gaaagcggtt tctgttttta cccagagcca ggtgacgaag ttattattgg
    12421 tttttttgaa aatgatccgc gttatccagt tattttaggt gctatgcata atccgaaaaa
    12481 taaggcccct tttgaaccaa cccaagataa tagggaaaaa gtattgatcg ttaaaaaagg
    12541 tgaagcgcaa caacaattag tcattgatgg caaagagaaa atgatccgaa ttaatgcggg
    12601 tgaaaatcaa ataatgcttc agcaagataa agacatttct ctgtcaacga aaaaagaatt
    12661 aacactgaaa gcgcagacaa tgaatgccac gatggataaa tcattggcaa tgtccgggaa
    12721 aaacagtgtt gaaatcaaag gcgcaaaaat taatcttacc caaggtggtt caggtggtgg
    12781 tggtagcggt ggtggcggtt ctggcggtgg cggtagtggc ggaggtggaa gcggaggcgg
    12841 tggcagtggt ggtgcaccga aaaagaaacg caaagttggt attcatggtg ttccggcagc
    12901 aatggataag aaatactcaa taggcttaga tatcggcaca aatagcgtcg gatgggcggt
    12961 gatcactgat gaatataagg ttccgtctaa aaagttcaag gttctgggaa atacagaccg
    13021 ccacagtatc aaaaaaaatc ttataggggc tcttttattt gacagtggag agacagcgga
    13081 agcgactcgt ctcaaacgga cagctcgtag aaggtataca cgtcggaaga atcgtatttg
    13141 ttatctacag gagatttttt caaatgagat ggcgaaagta gatgatagtt tctttcatcg
    13201 acttgaagag tcttttttgg tggaagaaga caagaagcat gaacgtcatc ctatttttgg
    13261 aaatatagta gatgaagttg cttatcatga gaaatatcca actatctatc atctgcgaaa
    13321 aaaattggta gattctactg ataaagcgga tttgcgctta atctatttgg ccttagcgca
    13381 tatgattaag tttcgtggtc attttttgat tgagggagat ttaaatcctg ataatagtga
    13441 tgtggacaaa ctatttatcc agttggtaca aacctacaat caattatttg aagaaaaccc
    13501 tattaacgca agtggagtag atgctaaagc gattctttct gcacgattga gtaaatcaag
    13561 acgattagaa aatctcattg ctcagctccc cggtgagaag aaaaatggct tatttgggaa
    13621 tctcattgct ttgtcattgg gtttgacccc taattttaaa tcaaattttg atttggcaga
    13681 agatgctaaa ttacagcttt caaaagatac ttacgatgat gatttagata atttattggc
    13741 gcaaattgga gatcaatatg ctgatttgtt tttggcagct aagaatttat cagatgctat
    13801 tttactttca gatatcctaa gagtaaatac tgaaataact aaggctcccc tatcagcttc
    13861 aatgattaaa cgctacgatg aacatcatca agacttgact cttttaaaag ctttagttcg
    13921 acaacaactt ccagaaaagt ataaagaaat cttttttgat caatcaaaaa acggatatgc
    13981 aggttatatt gatgggggag ctagccaaga agaattttat aaatttatca aaccaatttt
    14041 agaaaaaatg gatggtactg aggaattatt ggtgaaacta aatcgtgaag atttgctgcg
    14101 caagcaacgg acctttgaca acggctctat tccccatcaa attcacttgg gtgagctgca
    14161 tgctattttg agaagacaag aagactttta tccattttta aaagacaatc gtgagaagat
    14221 tgaaaaaatc ttgacttttc gaattcctta ttatgttggt ccattggcgc gtggcaatag
    14281 tcgttttgca tggatgactc ggaagtctga agaaacaatt accccatgga attttgaaga
    14341 agttgtcgat aaaggtgctt cagctcaatc atttattgaa cgcatgacaa actttgataa
    14401 aaatcttcca aatgaaaaag tactaccaaa acatagtttg ctttatgagt attttacggt
    14461 ttataacgaa ttgacaaagg tcaaatatgt tactgaagga atgcgaaaac cagcatttct
    14521 ttcaggtgaa cagaagaaag ccattgttga tttactcttc aaaacaaatc gaaaagtaac
    14581 cgttaagcaa ttaaaagaag attatttcaa aaaaatagaa tgttttgata gtgttgaaat
    14641 ttcaggagtt gaagatagat ttaatgcttc attaggtacc taccatgatt tgctaaaaat
    14701 tattaaagat aaagattttt tggataatga agaaaatgaa gatatcttag aggatattgt
    14761 tttaacattg accttatttg aagataggga gatgattgag gaaagactta aaacatatgc
    14821 tcacctcttt gatgataagg tgatgaaaca gcttaaacgt cgccgttata ctggttgggg
    14881 acgtttgtct cgaaaattga ttaatggtat tagggataag caatctggca aaacaatatt
    14941 agattttttg aaatcagatg gttttgccaa tcgcaatttt atgcagctga tccatgatga
    15001 tagtttgaca tttaaagaag acattcaaaa agcacaagtg tctggacaag gcgatagttt
    15061 acatgaacat attgcaaatt tagctggtag ccctgctatt aaaaaaggta ttttacagac
    15121 tgtaaaagtt gttgatgaat tggtcaaagt aatggggcgg cataagccag aaaatatcgt
    15181 tattgaaatg gcacgtgaaa atcagacaac tcaaaagggc cagaaaaatt cgcgagagcg
    15241 tatgaaacga atcgaagaag gtatcaaaga attaggaagt cagattctta aagagcatcc
    15301 tgttgaaaat actcaattgc aaaatgaaaa gctctatctc tattatctcc aaaatggaag
    15361 agacatgtat gtggaccaag aattagatat taatcgttta agtgattatg atgtcgatca
    15421 cattgttcca caaagtttcc ttaaagacga ttcaatagac aataaggtct taacgcgttc
    15481 tgataaaaat cgtggtaaat cggataacgt tccaagtgaa gaagtagtca aaaagatgaa
    15541 aaactattgg agacaacttc taaacgccaa gttaatcact caacgtaagt ttgataattt
    15601 aacgaaagct gaacgtggag gtttgagtga acttgataaa gctggtttta tcaaacgcca
    15661 attggttgaa actcgccaaa tcactaagca tgtggcacaa attttggata gtcgcatgaa
    15721 tactaaatac gatgaaaatg ataaacttat tcgagaggtt aaagtgatta ccttaaaatc
    15781 taaattagtt tctgacttcc gaaaagattt ccaattctat aaagtacgtg agattaacaa
    15841 ttaccatcat gcccatgatg cgtatctaaa tgccgtcgtt ggaactgctt tgattaagaa
    15901 atatccaaaa cttgaatcgg agtttgtcta tggtgattat aaagtttatg atgttcgtaa
    15961 aatgattgct aagtctgagc aagaaatagg caaagcaacc gcaaaatatt tcttttactc
    16021 taatatcatg aacttcttca aaacagaaat tacacttgca aatggagaga ttcgcaaacg
    16081 ccctctaatc gaaactaatg gggaaactgg agaaattgtc tgggataaag ggcgagattt
    16141 tgccacagtg cgcaaagtat tgtccatgcc ccaagtcaat attgtcaaga aaacagaagt
    16201 acagacaggc ggattctcca aggagtcaat tttaccaaaa agaaattcgg acaagcttat
    16261 tgctcgtaaa aaagactggg atccaaaaaa atatggtggt tttgatagtc caacggtagc
    16321 ttattcagtc ctagtggttg ctaaggtgga aaaagggaaa tcgaagaagt taaaatccgt
    16381 taaagagtta ctagggatca caattatgga aagaagttcc tttgaaaaaa atccgattga
    16441 ctttttagaa gctaaaggat ataaggaagt taaaaaagac ttaatcatta aactacctaa
    16501 atatagtctt tttgagttag aaaacggtcg taaacggatg ctggctagtg ccggagaatt
    16561 acaaaaagga aatgagctgg ctctgccaag caaatatgtg aattttttat atttagctag
    16621 tcattatgaa aagttgaagg gtagtccaga agataacgaa caaaaacaat tgtttgtgga
    16681 gcagcataag cattatttag atgagattat tgagcaaatc agtgaatttt ctaagcgtgt
    16741 tattttagca gatgccaatt tagataaagt tcttagtgca tataacaaac atagagacaa
    16801 accaatacgt gaacaagcag aaaatattat tcatttattt acgttgacga atcttggagc
    16861 tcccgctgct tttaaatatt ttgatacaac aattgatcgt aaacgatata cgtctacaaa
    16921 agaagtttta gatgccactc ttatccatca atccatcact ggtctttatg aaacacgcat
    16981 tgatttgagt cagctaggag gtgacaaacg tccggcagca accaaaaagg caggtcaggc
    17041 caaaaagaaa tgaaaggtga cgatgaatgg aaaatcaaat actgacacaa ctctatggtc
    17101 gtggttgggc ttttcctccg gtcttttccc ttgaaaaggg ggtagagatg gctgaagggg
    17161 cggaagatgt gagacaaagt ttgcagattc tgtttagtac tgagccgggg gaacgtctta
    17221 tgcgtgaaaa ttatggctgc ggattaaatg attttatgtt tgaaaatatc cgcaatgaac
    17281 ttattgctga aattgaatcc catatccatg acaacgtatt acgatatgaa ccccgggctg
    17341 atatgactga tattcaggtt cgtcaatccc ctggcatggg gaatactttg caagtgcagg
    17401 tcatgtatcg cctgagaggg agtgatatca atcaacaaat ccagggagta cttgcactga
    17461 gtgaaggccg ggtgacggag gtagtatgag tgaagcgatt gtggtggatg gtgacgtgtt
    17521 acagtttgat cccaactttg gcaatcggca ggtgacggtt cccagcccag gaaaaattag
    17581 cggcacagga catgcgcagg taagtggaaa aaaagtgtgt attctggggg atgagaaaca
    17641 ggtcagggtt tctgcaacct atattacaac aacacatact acgccgggaa caggaaccat
    17701 tactatcagt gctctggatg ctggccagca ggcccttcag tgtaccagtg gggcggcttt
    17761 aattatcaag gggcagcaat ttacggcgat gtttacgcct gaattgccag ccatgaataa
    17821 tacagtgact ccgccacaac cggatgttac gacaccttca tcaggaaaag gacgttttat
    17881 cactcaacaa aattttgcta ccgtaaatta gagtattgac tgaattaaat agaattaacg
    17941 aaggtgtaaa taattattta tttgctgacg aatcgctgtg acaaataaac acaggtgatg
    18001 ttatggaatt aaatgagtta actaacaaat tgtcaaattt ggtgccaatg accgatttta
    18061 aattagataa tcgagccagt ttgcaattgc ttaaatatat tgaagcgtat acgaagataa
    18121 taccctttaa ttctggcgat aaatattgga atgacttttt ctttatgtca ggaaatacgc
    18181 cagagaaact tgcaaaatta tatcagaaag aaatagaacc caatggggag ttattacctc
    18241 agcaggcttt tttgttggcg gttttgcgtt tattggaaac accaatatcc ttattaaatg
    18301 tattacctgc tgctcatcgt gagctctatt atcgggagct tttaggcttg tcttcccatg
    18361 cggcacagcc tgatcaggtt gctttatcta tggaactgaa ttcgacagtg atggaacagc
    18421 tgctccctga aggaaccctg tttgaggctg gtcaggatga acaaggcaat gcattgcaat
    18481 atgccctgga tgccagtttg ctggctaatc gtggatatat cagtgacttg cgctggttac
    18541 ggaatgacgg ggaaaagcaa tgggttactt ctgctccatg ggatttacag gcacaggtgt
    18601 cactgccgtc tgatgggata cgattatttg gtaagacaaa tagtgatcag caggtatttg
    18661 gtggggtgtt gataacgtca tcacttctgg cgatggaagc ggggataagg aagatcattg
    18721 ttacttttga gcaggagatg aacacccaag aactggtggc acaggtcagc agtggaaatc
    18781 aatggctaac attgacgtct gaggtaaata agaaagaggt cacactgaca ctgtcagaca
    18841 aagaaccggc aatcagtgcg ccagaggatc tggataatct ctttttcacg caaccggtac
    18901 tcaggctaca gggaaaggat agtcaggcac tgccggaggt gacgggtatc agcgtttcgg
    18961 aaaaggatga tactaaggat acctcttttg agatgtatca cttaacacca tttggttata
    19021 gcagtgatat agagccattg gaggaaaatc cagcgttata tttaggcttt actgatgtaa
    19081 agccagggca aacactggcg ctgtattgga aattaaaatc cccgcagcaa ccaaccgttt
    19141 cctggtatta cctggatcaa cataatcaat gggctgaatt ggattcatgg gtcagtgatg
    19201 gaacccagaa tctgtatcag gatggtactt ggcacgttga gttgcctgtg gatgcatcca
    19261 atcaggcaga gcagatgcca gttggacgct attggttgcg ggcagtggtg gaggtacccg
    19321 ctcatgaggg ggcgttgggg aaggctcctt ggctatatgg tctaatctat aacgccatga
    19381 cggcaacctt ggttaatgta gatagcatca gtgacagcca tttcttaacc cctttgcctg
    19441 ccagcagcat acagcggccc gttgaaccca tcattgtgtt ggcatcggtc aaccagcctt
    19501 gggcatcatg gggtggacgt atacctgaat cctacagtgc cttttttgaa cggatagctc
    19561 aaaacctgtc tcatcgaaac cggtccttaa cctggggaaa tatggtgaca ttactcaaag
    19621 agcgttatgt cagcatcttt gatgttaagt atccaggtaa tgatgaactc accagagtgc
    19681 cagcattgga gcagcagcaa ctaacagtga ttccagcaaa ccggtacaac gatagcgatg
    19741 attctctgcg tccggtactg aatcctgctc gtctgcaaga gatggctgat tggttgcagc
    19801 agaaagactc tccctgggcc tctattgagg tcaggaatcc agaatacttg gatgtgaaaa
    19861 tccattacga ggtgattttt aaacctgatg tgaacgaaga ttttggctat cgccagctac
    19921 agcagcaact gtgtgaggtg tatatgcctt ggagcataga tgagcagcgg cccgttgtat
    19981 tgaataacag cattaattat ttccagttgt tagccactat tcaacagcaa ccgctggttg
    20041 agcgagtcac tcgtctgaca ctacatcggg ctgattcttc tgatgagagt gatggtacag
    20101 catctgtgga agccaaagat aatgaagtgc ttattttagt ctgggaagag gacgataatc
    20161 tgcaataccg aggaaatgac tatgagtaat caggatgcac tgtttcatag cgttaaagac
    20221 gatattcact ttgatacctt gctggaacaa gctcatcagg tgattgaaaa acaggctgaa
    20281 aaactgtgga gtgatacggc agagcatgat ccgggtatca catttttgca gggaatcagt
    20341 tacggtgtgt cagatttggc ttaccgacat acattacccc tgaaagattt actgactcca
    20401 gcgccggatg agcagcagca agagggaatt tttcctgccg aatttggccc gcataataca
    20461 ctgacttgtg ggccggtgac agcggatgat tatcgcaagg cattgttaga tctacacagc
    20521 agcgacagcc tggatggtac tcagcaggat gagggggatt ttctgttccg gagtgtgcaa
    20581 ctggtgcgtg aaccggaaaa acagcgttat acctattggt atgatgcaac caagagggaa
    20641 tatagctttg tcaacagtga aggggctaaa gagtttacct tgcgggggaa ttactggttg
    20701 tatctggaac caacccgttg gactcagggt aatattgccg ctgctaccag acaactgaca
    20761 gaatttttga ctaaaaatcg caatattggt gaatctgtca gcaacattat ctggctacaa
    20821 ccggttgatc tgccactgtt gctggatgtt gaactggatg atgatgtagg tgcacaggat
    20881 gtccccggta tttttgcggc ggtgtatagc accgcagagc agtatctgat gcctggagca
    20941 cagcgttacc gtacggaagt actgcaaaat gctgggatga gcaatgatca aatcttcgaa
    21001 ggtccattat tggaacatgg ctggatacca gagctgccgg cagcccgtga ttatactcaa
    21061 aggctcactc tcaatcttag ccggttggta aatagtctgc ttgagattga gggcattaaa
    21121 catgtgaatc gtcttcgtct ggatgatagc ttcgataaaa ctgctattga acccgttaag
    21181 ggggatacct ggtcgtggtc gatcaaagag ggctattatc cacgtctttg gggagaagac
    21241 ccacttaacc aattggcgca acaaaatggc ccgcttaggg tgatagccaa aggagggatt
    21301 agcgtcagtg tgagtaaaga gcaaatccag gccagtttac ccagtcaatc actgattcaa
    21361 aatgagccgg taatattggc ttacggccag caccgtgacg ttggcagcta ttatcccgtc
    21421 agtgatactt tgccgccttg ctatggacta caacattctt tgtctgaaag tgaacactta
    21481 ttgccacttc atcaatttat gttgccattt gaacaattat tggcctgtgg ttgtcaacag
    21541 atagccatgc tcccgcggtt actggctttt cagcgcgaag gttatgaggt ttggggtgat
    21601 cagtggccct ttaagtcagg ctcagtgaat gatgacgccc atcaagatta tgcccctgca
    21661 ttaaaggatt tgttaggaca gattgcgctg gatagtgatc atgaattgga tattattaat
    21721 tacttgctgg gttactttgg cacacagcgg gcaccgcgta cctttacgac acaactcgat
    21781 gattttcgtg cggtccaaca gggttatctg gcccagcaac cgacattgac ttaccaccgc
    21841 tccaatattc gtatcgatca ggtatcgtcg ctacaaaaac gtattgctgc tcgcatgggg
    21901 ctgggcggtg agttgtttaa acctcaaccg gatctgagcc aactgccttt ttatttgatt
    21961 gaacatcgag cgttgctgcc agtcaaaccc aatagtcagt ttgataagga acagaaacca
    22021 gcctcggtga cagaggaggg gggcagccaa acaggtcaac attatgtggt cattgaacag
    22081 aagggcattg atggcaagct gacacagggg caagtgatca atttaattct gtatgaagga
    22141 gagcagggag aaacccaatt tacgatacgc ggtcagatgg tattcaaaac cgagggggat
    22201 aagttttggt tggatgtgaa taatagtgcg caactggaat ataatctggc gcgggtaatg
    22261 acagcagcca aggcgagtaa actcttttgg caaaacagcc cggtatggat ggaggatatg
    22321 ggctatcgtc tggcctatgc tagtgaccaa tcctcattgc ctgtgaatca acggcgcttg
    22381 acccgcacag tgcaaactcc attcccgccg atggttgttg taggtagcga aatcaccctg
    22441 ttaaagcagg tggggatagt caatttaaaa aaagcggagt cagaaaaact ttatgcaaaa
    22501 gttgttagct ttgatcgcat tgaagggacc ttgattattg agcgtttggg taattccact
    22561 ctggcttttc ctacctcgga agaggcgtgg cggtatagtt ggtatttttc gggggagaaa
    22621 tatgaaagga ctgaccgctt ttcatttgtg attagcgtag tagtgaacag tgacttaatt
    22681 aaattgcccg gtgttgatcc ctataaattg gaagaatggg tgaaagaaac gattcttacc
    22741 gaatttccag ctcatatttc tatgattatc cattggatgg atcgggaagc ctttttaaat
    22801 ttcgccaata cctatcagcg ttggcaaaat aatggtacgc cactggggga tgcggcttat
    22861 tccattctag aaagtttgac acttggtaaa ttgccatctg ccttaaaagg tgttggcaca
    22921 atgcgtattg ccacatctag tcaaagagaa gaagtggtgg gtagtaatgg tgatcaatgg
    22981 aatacagatg gaataaccca gaatgaatta ttctatgttc ctaaagagag ctaggaaaaa
    23041 taaatatctg ccactaatga tgttgaatta aatatgtttt ctggagttaa tcatgaacga
    23101 aactcgttat aatgcaactg tacaagaaca acaaacatta tctaatccaa aagctgttgg
    23161 acctgacatc gataaattaa aggataaatt taaagagggc agtattcccc tgcaaaccga
    23221 tttcaatgag ttaattgata ttgccgatat tggacgtaaa gcctgtggtc aagcgccaca
    23281 acaaaatggc ccaggagaag gattgaaatt ggctgatgac ggtacgctta atttaaaaat
    23341 aggcactttt tccaataaag acttttctcc attaatatta aaagatgatg ttttatctgt
    23401 agatcttggt agtggtctga ctaatgaaac caatggaatc tgtgtcggtc agggcgatgg
    23461 tattacagtt aacactagca atgtagctgt aaaacaaggt aacggaatta gcgttactag
    23521 tagtggtggt gttgccgtta aagttagtgc taataaggga cttagcgttg atagtagtgg
    23581 tgttgcagtt aaagttaata ctgataaggg aattagcgtt gatggtaatg gtgttgcagt
    23641 taaagttaat actagtaaag gaattagcgt tgataataca ggtgttgcag ttatagctaa
    23701 tgctagtaag ggaattagcg ttgatggtag tggtgttgca gttatagcta atactagtaa
    23761 aggaattagc gttgatggta gtggtgttgc agttatagct aatactagta aaggaattag
    23821 cgttgataat acaggtgttg cagttatagc taatgctagt aagggaatta gcgttgatgg
    23881 tagtggtgtt gcagttatag ctaatactag taaaggaatt agcgttgatg gtagtggtgt
    23941 tgcagttata gctaatacta gtaaaggaat tagcgttgat agtagtggtg ttgcagttaa
    24001 agttaaagct aatggcggaa ttaaagtaga tgctaatggt gttgcaattg atcctaataa
    24061 tgtactcccc aagggagtga ttgtaatgtt ctctggcagt actgcaccaa ctggttgggc
    24121 gttatgtgat ggcaataatg gtacaccaaa tttaatcgat cgatttattt taggtgggaa
    24181 agggactgat attaatggag tgagtactaa tacagcttca ggtactaaaa atagtaagtt
    24241 attcgatttc agttctgatg aagctacatt aactattgat ggtaaaacac tggggagagg
    24301 cggttctggc ggcggtggtt ctggcggtaa cgataaactg accctgtgga ccacaccggc
    24361 accgagtccg aattgtcgtc tgaatgcaga aaaagatgca aaactgacac tggttctgac
    24421 caaatgtggt agccagattc tggcaaccgt tagcgttctg gcagttaaag gtagcctggc
    24481 accgattagc ggcaccgttc agagcgcaca tctgattatt cgttttgatg aaaatggcgt
    24541 gctgctgaat aatagctttc tggatccgga atattggaat tttcgtaatg gtgatctgac
    24601 cgaaggcacc gcatatacca atgcagttgg ttttatgccg aatctgagcg catatccgaa
    24661 aagccatggt aaaaccgcaa aaagcaatat tgttagccag gtgtatctga acggcgataa
    24721 aacaaaaccg gttacactga ccattacact gaatggcacc caagaaaccg gtgataccac
    24781 accgagcgcc tatagcatga gctttagctg ggattggagc ggtcataact atatcaatga
    24841 aatttttgcg accagcagct acacctttag ctatattgca caagaaggtg gctctggtgg
    24901 tggtggttca ggtggtcacg atcatgatat taagataacg ggcacaggaa aacattctca
    24961 caaaaacaaa gtaacagtcc cttattatat tctggctttc atcataaagc tttaatatat
    25021 atgaaaaatt gaaaatataa attatccatt aataataaag aggatattag catgacttcg
    25081 gagccaaatc tgttaaaccg gattacaatt actattgaag ctaataatca acaagtagct
    25141 agaaaagtat tgcatggctc cttgcttaat caagctaata taaataaatt atttaattca
    25201 tactttaatg aatatgaaat taataggggt gtttatttag aaacattaat cctgaatctt
    25261 ggtacgataa atttccatga ttttaattca ttgtttccta ctctcctaaa agctgcattg
    25321 aataaagaat tcagtcaata tcagataaac aaccataggg aagaaatgct atttaatgag
    25381 acaatatcaa atcaagctac tgataagtct tacatatttg gcgataacaa attaattgat
    25441 gcagagaatt tcattcactt tttatatcaa aagcattcca cattaaatct agtagaagca
    25501 atgggaaata atggtattga aaaattaaca aatcagttaa cacaaataga aaataaattt
    25561 gcgttattat tggcaaaaag ttgtttgtct gaggaaggct taaaacgact cttggctatc
    25621 aaacaacccg atttattaat cgctatcaat cgcagattat ctgaaagaat aaatagacca
    25681 caatatcagg agaagcttgt ttcctgcgga caactgatat ttagtgctct gggatatata
    25741 caacagtaca atatacagga aattcctaaa ccggatgaaa aagttattgc acgcataaca
    25801 actgaactta ataataatgg tttgcttaat acaataccta ttattacact atttcgtcag
    25861 agtgggatta acgattcatc actaaatgat tggctaaaga aaatctggca ggtgagatca
    25921 atttcacagt tatgcagaaa gtatctttct gctaaggaat accaatatct gtcagaacat
    25981 tttgtttcaa agagcgtcga taaaaataga tatgatgaag agcccgtaaa tcagagcata
    26041 ttatcaaggt tgaataataa ttccattaaa gaaggaaata atcacagtca actctgtact
    26101 ctcagtagac tatattctga acccgttgta ttacctgaac aaaccattct acgtcaggtt
    26161 agtaatacag tagatcagag catattatca aggttgaata atgcctccat taaagaagga
    26221 aataaccaaa gtcaacttcg cactctcagt agactatatt ctgagcccgt tgcattacct
    26281 gaacaaacca ttccacgtca ggttagtaat acaggtatat taattctatg gccaatgcta
    26341 cctacactat ttaaccagct tggtctactt gagaaaaaga aatttatcca tcgtcaggcc
    26401 cagtttaatg ccgttgattt tcttgattac ctgatttggg gaaccgaaga tgtgaaagtg
    26461 gaacgaaagg ttttgaataa tgttctatgt gggttaatgg ctgatgaaat tactgaacca
    26521 atgcctattg aaccagaaaa acaatggata ataattcaat ggctggacgc tattatctcc
    26581 caactttctg gctggaaaaa gttaagtcgt aatgacgtcc gtcaattatt tctacaacga
    26641 ccaggagaat tactgatcaa tgaacaggaa attaaaatca caatacagca acaaccattt
    26701 gatgctctgt taactgattg gccgtggcca atgaatatgg cttgttttag ctggttgagt
    26761 caaccattaa ccattacgtg gttataacca ttgaccacaa tgacttagtc tgagtaaaaa
    26821 atatgaatat atcgcctgtt ttttatgatt cattgaatca ggataacgac cgtgatctat
    26881 cgtttttatt tagcgaactg gaacgaatag atctcgctct tcaacaccat ttttattgtg
    26941 tagaaagtca gcgaagtgag ctcctggatg agtttctgct cactgaggcg gaagtggtga
    27001 ccaggctgga taagccactt ggtaaacctc attggataaa tgatgattat ctggcgatat
    27061 cgcaaaaggg caatgtaagc ctaatggcag cgtccagatt aatggatctg atcgaacgct
    27121 ttgaactgac tgattttgag cgcgatgttt tactattagg cttattgccc cattttgata
    27181 gccgctatta tcgactgttt tcgctgattc aagggggaca acagggtcga ttaccttctt
    27241 ttgcgctggc attggaactg ttttgccact cggcgctgga gaaacaggta cagcaagcga
    27301 gttttctgca ccgggcacct ttgatgggtt gccagctatt atccatcgat actagtcaaa
    27361 aaacgctggc ctggctccag actcccttta ttactgacag cggggtatat cactttttac
    27421 tggggcatca ctacattatg ccggctttag aacattgtgc tgagtggtta acaccgacag
    27481 ggattggctg ttatcctgaa ggattaaaac aagtactggg taacgtattg ttatctgaca
    27541 acgataatat tagaccgatt gtcttattac ggggaatggc cggcagtgcc agagcttata
    27601 ccattactaa tatgatggct tcagaaggga agcaaacact gctggtagat atatccaaac
    27661 ttgctgatag cgatgaaaaa aacattattc ttcagataaa gcatattttg cgggaaaccc
    27721 gcatgcatgg agcatgttta ttattacgga atttttgctt gttagtggaa cagaataaac
    27781 aactattgga ctccctgtca gagttattga atcaacctga attaagaatt gtttgcctga
    27841 ttgagcctta ttccccattg gtatggctga aaaagatacc ggtattactg attgagatgc
    27901 cacttttaac gcctgcggaa aaagccagat tgttaattgc cagcttaccg gataattgtt
    27961 ccgaggatat tgatacgata actttaagcc agcgttacac ttttaaccca gaaaccctgc
    28021 cattgatttt gcaagaggcc cagctttatc aacagcagcg agatccgctg gatatcttgc
    28081 agcaatgcga tatacgccag gcattaaatt tgcgtgctca acaaaatttc ggtcaattgg
    28141 cacagcggat tattcctaag cgctcattaa aggatttatt ggtatccgat gagattgctc
    28201 agcagttacg ggaaatactc ataacaatta agtatcggga acaggttctg gcgggagggt
    28261 ttaaagataa aattgcctat ggcactggta tcagcgccct gttttatggt gattcaggca
    28321 ctggaaaaac catggcagca gaagtgattg ctgaccacat tggcgttgac ttaataaaag
    28381 tggatttatc tacagtagtg aataaataca tcggtgaaac agaaaaaaac ttatcccgta
    28441 ttttcgattt ggcggaacag gatgcagggg tattattctt tgatgaagct gacgcactgt
    28501 ttggtaaacg cagtgaaact aaagattccc aggacagaca tgccaatatt gaagtttctt
    28561 acttattaca gcgcctggag aattacccgg gtctggtcat tttatccacc aataatcgtg
    28621 gtcatttaga cagtgctttt aatcgtcgtt ttactttcat tacccgtttt acttacccgg
    28681 atgaaaaaat ccgtaaaaaa atgtggcagg aaatttggcc tagaaatata aaaatatcgg
    28741 aagatatcga ttttaacgaa ttagctcaac gaacaagcgt gactggcgcg aatatccgca
    28801 atattgcttt attgtcttca ttctttgctt cagagcaggg gaatgatgaa gtcagtaatg
    28861 aaaatattga aattgcattg aagcgtgaat tagctaaagt cggacgatta acattttaaa
    28921 agttatcaca atgaaagtat tgaaatatta aataaattta ttaccaaaaa gttatcacga
    28981 tataatttaa gagaggtttt ttatgttaaa cacgcaaact attattgatg tcaataaggc
    29041 aatggatgcc atgctgcgcg catatctgaa tcaagatatt gccattcgtt ttgatctacc
    29101 tgaattggat actatgcaat ctgatgcgat ggtaagtatc tttctttatg acattcatga
    29161 agatttacag cttcgctcgg cagaatcaag agggtttgat gtttatgccg ggaggttatt
    29221 gcctggttgg gtaaatatta aatgtaacta tctgattacc tattgggaag cttctaagcc
    29281 agcgactgat gccagcagtc cggatagcca acctgataac caggcaatac aagtgatgtc
    29341 acaagtatta aatgccttga ttaataatcg tcaattggca ggtattcctg gtgcttatac
    29401 tcaggttgta ccgcctaaag agagtttaaa tagcctgggg aatttctggc aatcactggg
    29461 taatcgccca cggctttctc tcaattattc agtgacagta cctgttagcc taaacgatgg
    29521 tcaggatagc gcgactccgg ttaccgcggt ttcttctaca gtggaacaaa cggcatcgct
    29581 cagtcaagaa gtggttagtc atgctttacg cgaattactc attacggaat taggaggagg
    29641 agaggataac cggttggtac tgagtaaagt tgaattatcc gcagtgaaag agacgatgac
    29701 tcaagacagt ccggctcaga tgattatatt gttgtctgtt tcaggcatta cacgacagga
    29761 atatttgaag gaaattgata atatctttga tcgttgggta aataatgctg aagttattac
    29821 cactattgat gattgtggga ttagaattga aagtataacg aaagataatc ttgtaggaat
    29881 ttaaattttt ttaattaaat aaaggaatta atattgattt gatagagcat gtaatcaata
    29941 acttctatga aaatgttgaa aaacaataca cggaaacact taacttacaa ttaaattgtt
    30001 aaag
//
