LOCUS       sequence-487011-       30012 bp    DNA     circular UNA 15-DEC-2025
DEFINITION  PVC structural operon - tail fiber (Pvc13) retargeted with Ad5 knob 
            - spike tip (Pvc10) loaded with Cas9 on NTD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Kreitz J, Yang V, Friedrich MJ, Pham J, Macrae RK, Zhang F
  TITLE     Targeted delivery of diverse biomolecules with engineered bacterial 
            nanosyringes.
  JOURNAL   Nat Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x.
  PUBMED    40796978
REFERENCE   2  (bases 1 to 30012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2025 Aug 12. doi: 10.1038/s41587-025-02774-x."
COMMENT     Sequence Label: pAWP78-PVCpnf_pvc13-Ad5_Cas9-Pvc10
FEATURES             Location/Qualifiers
     source          1..30012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(339..1154)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(339..1154)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1062..1081
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1146..1165
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(1458..2606)
                     /codon_start=1
                     /product="trans-acting replication protein that binds to 
                     and activates oriV"
                     /label=trfA
                     /translation="MNRTFDRKAYRQELIDAGFSAEDAETIASRTVMRAPRETFQSVGS
                     MVQQATAKIERDSVQLAPPALPAPSAAVERSRRLEQEAAGLAKSMTIDTRGTMTTKKRK
                     TAGEDLAKQVSEAKQAALLKHTKQQIKEMQLSLFDIAPWPDTMRAMPNDTARSALFTTR
                     NKKIPREALQNKVIFHVNKDVKITYTGVELRADDDELVWQQVLEYAKRTPIGEPITFTF
                     YELCQDLGWSINGRYYTKAEECLSRLQATAMGFTSDRVGHLESVSLLHRFRVLDRGKKT
                     SRCQVLIDEEIVVLFAGDHYTKFIWEKYRKLSPTARRMFDYFSSHREPYPLKLETFRLM
                     CGSDSTRVKKWREQVGEACEELRGSGLVEHAWVNDDLVHCKR"
     CDS             complement(2878..3249)
                     /codon_start=1
                     /gene="traJ"
                     /product="oriT-recognizing protein"
                     /label=traJ
                     /translation="MADETKPTRKGSPPIKVYCLPDERRAIEEKAAAAGMSL*AYLLAV
                     GQGYKITGVVDYEHVRELARINGDLGRLGGLLKLWLTDDPRTARFGDATILALLAKIEE
                     KQDELGKVMMGVVRPRAEP"
     oriT            3282..3391
                     /note="incP origin of transfer"
     rep_origin      3544..4175
                     /label=oriV
                     /note="incP origin of replication"
     rep_origin      4484..5072
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4973..4992
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             13210..13230
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             13255..17358
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             13255..17358
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
ORIGIN
        1 gagatgatga taagaatggt cagtcaccag ataaaatgat ctctggtcaa ttttaggaaa
       61 gctgaaaaaa cctgaccaaa tgcgtcgtaa attctatgct tagaaagact cggtgccagt
      121 gtaataccta agatatggtt tttatttcca tatctgttcg aaaacttaga aataacaagt
      181 tcaacaataa ccagggactt taaatatcgg atccttgtcg ggaagatgcg tgatctgatc
      241 cttcaactca gcaaaagttc gatttattca acaaagccgc cgtcccgtca agtcagcgta
      301 atgctctgcc agtgttacaa ccaattaacc aattctgatt agaaaaactc atcgagcatc
      361 aaatgaaact gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt
      421 ttctgtaatg aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat
      481 cggtctgcga ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa
      541 ataaggttat caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa
      601 agcttatgca tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa
      661 tcactcgcat caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg
      721 cgatcgctgt taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact
      781 gccagcgcat caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct
      841 gttttcccgg ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc
      901 ttgatggtcg gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta
      961 acatcattgg caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc
     1021 ccatacaatc gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac
     1081 ccatataaat cagcatccat gttggaattt aatcgcggcc tcgagcaaga cgtttcccgt
     1141 tgaatatggc tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt
     1201 catgatgata tatttttatc ttgtgcaatg taacatcaga gattttgaga cacaacgtgg
     1261 ctttcgactt ccggcaagct atacgcgccc tagaattgtc aattttaatc ctctgtttat
     1321 cggcagttcg tagagcgcgc cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga
     1381 gcagtgcccg cttgttcctg aaatgccagt aaagcgctgg ctgctgaacc cccagccgga
     1441 actgacccca caaggcccta gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt
     1501 tccaccaggc cgctgcctcg caactcttcg caggcttcgc cgacctgctc gcgccacttc
     1561 ttcacgcggg tggaatccga tccgcacatg aggcggaagg tttccagctt gagcgggtac
     1621 ggctcccggt gcgagctgaa atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg
     1681 tacttctccc atatgaattt cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg
     1741 tcgatcagga cctggcaacg ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc
     1801 agcagcgaca ccgattccag gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc
     1861 tgtaggcgcg acaggcattc ctcggccttc gtgtaatacc ggccattgat cgaccagccc
     1921 aggtcctggc aaagctcgta gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc
     1981 gcgtactcca acacctgctg ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg
     2041 gtgtaggtga tcttcacgtc cttgttgacg tggaaaatga ccttgttttg cagcgcctcg
     2101 cgcgggattt tcttgttgcg cgtggtgaac agggcagagc gggccgtgtc gtttggcatc
     2161 gctcgcatcg tgtccggcca cggcgcaata tcgaacaagg aaagctgcat ttccttgatc
     2221 tgctgcttcg tgtgtttcag caacgcggcc tgcttggcct cgctgacctg ttttgccagg
     2281 tcctcgccgg cggtttttcg cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc
     2341 gacttcgcca aacctgccgc ctcctgttcg agacgacgcg aacgctccac ggcggccgat
     2401 ggcgcgggca gggcaggggg agccagttgc acgctgtcgc gctcgatctt ggccgtagct
     2461 tgctggacca tcgagccgac ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg
     2521 cttgcgatgg tttcggcatc ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat
     2581 gccttccggt caaacgtccg attcattcac cctccttgcg ggattgcccc gactcacgcc
     2641 ggggcaatgt gcccttattc ctgatttgac ccgcctggtg ccttggtgtc cagataatcc
     2701 accttatcgg caatgaagtc ggtcccgtag accgtctggc cgtccttctc gtacttggta
     2761 ttccgaatct tgccctgcac gaataccagc tccgcgaagt cgctcttctt gatggagcgc
     2821 atggggacgt gcttggcaat cacgcgcacc ccccggccgt tttagcggct aaaaaagtca
     2881 tggctctgcc ctcgggcgga ccacgcccat catgaccttg ccaagctcgt cctgcttctc
     2941 ttcgatcttc gccagcaggg cgaggatcgt ggcatcaccg aaccgcgccg tgcgcgggtc
     3001 gtcggtgagc cagagtttca gcaggccgcc caggcggccc aggtcgccat tgatgcgggc
     3061 cagctcgcgg acgtgctcat agtccacgac gcccgtgatt ttgtagccct ggccgacggc
     3121 cagcaggtag gcctacaggc tcatgccggc cgccgccgcc ttttcctcaa tcgctcttcg
     3181 ttcgtctgga aggcagtaca ccttgatagg tgggctgccc ttcctggttg gcttggtttc
     3241 atcagccatc cgcttgccct catctgttac gccggcggta gccggccagc ctcgcagagc
     3301 aggattcccg ttgagcaccg ccaggtgcga ataagggaca gtgaagaagg aacacccgct
     3361 cgcgggtggg cctacttcac ctatcctgcc cggctgacgc cgttggatac accaaggaaa
     3421 gtctacacga accctttggc aaaatcctgt atatcgtgcg aaaaaggatg gatataccga
     3481 aaaaatcgct ataatgaccc cgaagcaggg ttatgcagcg gaaaagatcc gtcgaccctt
     3541 tccgacgctc accgggctgg ttgccctcgc cgctgggctg gcggccgtct atggccctgc
     3601 aaacgcgcca gaaacgccgt cgaagccgtg tgcgagacac cgcggccgcc ggcgttgtgg
     3661 atacctcgcg gaaaacttgg ccctcactga cagatgaggg gcggacgttg acacttgagg
     3721 ggccgactca cccggcgcgg cgttgacaga tgaggggcag gctcgatttc ggccggcgac
     3781 gtggagctgg ccagcctcgc aaatcggcga aaacgcctga ttttacgcga gtttcccaca
     3841 gatgatgtgg acaagcctgg ggataagtgc cctgcggtat tgacacttga ggggcgcgac
     3901 tactgacaga tgaggggcgc gatccttgac acttgagggg cagagtgctg acagatgagg
     3961 ggcgcaccta ttgacatttg aggggctgtc cacaggcaga aaatccagca tttgcaaggg
     4021 tttccgcccg tttttcggcc accgctaacc tgtcttttaa cctgctttta aaccaatatt
     4081 tataaacctt gtttttaacc agggctgcgc cctgtgcgcg tgaccgcgca cgccgaaggg
     4141 gggtgccccc ccttctcgaa ccctcccggc ccgctaacgc gggcctccca tccccccagg
     4201 ggctgcgccc ctcggccgcg aacggcctca ccccaaaaat ggcagccaag ctgacccgct
     4261 agggacgtga agtcgattcc ttcgatggtt agcaatcaaa gactcagagt gctgtctgga
     4321 gcgtgaatct aacggtacgt atctcgattg ctcggtcgct attcgcactc tgcgaaagtt
     4381 cgtaccgctc attcactagg ttgcgaatca tgaccaaaat cccttaacgt gagttttcgt
     4441 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     4501 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     4561 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     4621 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     4681 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggtgataagt
     4741 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     4801 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     4861 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     4921 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     4981 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5041 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5101 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5161 tggataaccg tattaccgcc tttgagtgag ctgtcgcgaa tgcatctaga tataattaca
     5221 tcttcatcat tcggtatttt aaatgacata tatcctcctt ccgaatagtt ttaatggtga
     5281 tgttcgattg gacagtgttt tccattatta aaattttaag tatcacatca tgtttaatga
     5341 aggattgatg tttattagaa tattcataat agctttgtct ttgcctctat gtaaatgaaa
     5401 ttgcataaaa agcaaattgc attattggca atgtacttat tctggatttt tcggcacgat
     5461 aaattattgg tgtaagtttt atatcatgag taaaaattcg gaatgatatt cctgtcattt
     5521 tgtggcggta gcgttactca agatattatc ctacatcata tgttgattat tgtaatcaat
     5581 cgagctattt tttgtctaaa aaggggcttt tatgtctaca agtacatctc aaattgcggt
     5641 tgaatatcct attcctgtct atcgctttat tgtttctgtc ggagatgaga aaattccatt
     5701 taatagtgtt tcaggattag atattagtta tgacaccatt gaataccgag atggtgttgg
     5761 taattggttc aaaatgccgg gtcagagtca gagcactaat atcaccttgc gtaaaggcgt
     5821 tttcccgggg aaaacagaac tgtttgattg gattaactct attcagctta atcaggtaga
     5881 gaaaaaggat attaccatca gtttaactaa tgatgcaggt accgaattat taatgacctg
     5941 gaatgtttct aatgcttttc ccacttcatt gacttcacct tcatttgatg ccaccagtaa
     6001 tgatattgca gtacaggaaa ttacgctgat ggcagatcgg gtgattatgc aggctgtttg
     6061 aagcattgat atttaatcat ctcatataag ggaactttta tgacaaccgt taccagttat
     6121 cctggcgttt atattgaaga attaaatagc ctggccttgt cagtttcaaa tagcgccaca
     6181 gcggttcctg tttttgctgt ggacgaacaa aaccaatata ttagtgaaga taatgcaatc
     6241 cgtattaatt cgtggatgga ttatcttaat ctgattggca attttaataa tgaagacaaa
     6301 ttagatgttt ctgtgcgtgc ttattttgcc aatggaggtg gatattgtta tctcgtcaaa
     6361 acaacgagtt tagaaaaaat tattccaacc ttggatgatg taaccttatt ggttgctgcg
     6421 ggcgaagata ttaaaacgac agtagatgtt ttatgtcagc caggaaaagg gttattcgca
     6481 gtctttgatg gccctgaaac agagttgact atcaacggtg cggaagaggc aaaacaagcc
     6541 tataccgcca caccattcgc tgcggtttat tatccttggt tgaaagcgga ttgggctaac
     6601 atagatattc cacccagtgc agtgatggcg ggagtttatg catcggtgga tttatcccgt
     6661 ggtgtatgga aagcgcctgc caatgttgcg ttgaaagggg gcctggaacc taaattttta
     6721 gtcacggatg aattgcaggg tgaatataac actggccgcg ctatcaatat gattcgtaat
     6781 ttcagtaaca caggtactac ggtttggggt gcaagaaccc tggaagataa agacaattgg
     6841 cgttatgttc cagtgcgacg cttgtttaat tctgtggagc gggatatcaa gcgtgccatg
     6901 agctttgcta tgttcgagcc taataatcag cctacttggg agcgggtacg ggcggcgatt
     6961 agcaactacc tttatagcct gtggcaacag gggggattag ctggcagcaa agaagaagac
     7021 gcttattttg tgcaaattgg taaaggtata acgatgacac aggagcagat tgatgcaggg
     7081 caaatgattg ttaaagtcgg tttggctgct gtacggcctg cggaatttat cattctccag
     7141 tttacgcaag atgtagaaca gcgttaatca tatgattatg aggagttatc atgtctgcta
     7201 ttctgaaagc gcctggcgtt tatattgaag aagacgcttc cctagcgttg tctgtcagta
     7261 acagcgcgac tgccgtgcct gtttttatcg gaaaatttac tccgacagtg gttgattcaa
     7321 tccaagtctg tacccgtatc agcaactggc ttgaattcac ttcctctttt tccctagctc
     7381 caacagttga gattgttgtc caatctaaca ctgaatctga atctgaatct gaaacttacc
     7441 actatattga gacaatcaat ctatctccag ctgtggaagc attgcgactc tattttcaaa
     7501 atggcggagg agcttgctat atctacccat taaatgatgc tgaagatgaa ttggttctgg
     7561 cggccatacc agaagtcatt gaacagaaag gtgatattac tctgttggtt tgcccggaac
     7621 tcgatctgga ttacaaaact aagatctatg gcgcagtgag ctcactgttg aatgataaca
     7681 aagtgggcta tttcctgatt gcggatagca atgatggaga atctgtgtca ggagtatgga
     7741 atagtgctaa ggccgccgcc tattatcccc agttggaaac taacctaaaa ttttccacgt
     7801 tgcctgggga taaggacatt cgtatcagcg gttatcagga tgatgatgaa acacataaac
     7861 cgaaaaactt ggatgagctc aggacaatca acgaggcgtt ggcacaggat attgatgcaa
     7921 gattgctcga ggagaaacaa cgtgctgtca tcattccgcc aagtgctgcc attgcgggca
     7981 tttattgcca aacggataat cgtcgcggtg tttggaaagc gccagccaac gttgcgctca
     8041 cagggatcgg gagtttgctt gataaggtag acgatgaacg gcagggagag atgaatgaca
     8101 agggaatcaa tgtcatccgt tcatttaccg accgtggttt tatggtctgg ggagcccgta
     8161 cttgtgtgga cgctgccaac atcagctggc gttatattcc tgttcgtcgc ctgttcaatt
     8221 ccgttgaacg agatatccgc caggcgctac gcgctgtgtt gtttgaaact aatagtcagc
     8281 ctacctgggt acgtgctaag gctgccgttg atcaatatct ttataccctt tggcagaaaa
     8341 atgcattgat gggtgctcgc ccggaagaag cttattttgt gcaaattggt caggatatca
     8401 ccatgtccga ggctgatatt aaacagggta agatgatcat gactgttggt ttggcagcag
     8461 tgcggccagc tgagttcatc attctgcaat ttacgcagga tgttgttcag taatctccat
     8521 gactaaacgc caggcactgt attgacagtg cctactctaa ccatcttgga ggaggtgatg
     8581 atgatggaga gactccaacc gggtgtgact ttaacagaaa gtataatcac gatgggtcag
     8641 caagagatac ccagtgctgt gccggtgttt attggttaca ccgttcgtta tccggaacaa
     8701 tcggaagcat cagtccgtat cgacagtttg gccgagtata ccagcctgtt tggtgacgac
     8761 catgtgatga tgtttgctgt caggcactat tttgataatg gcgggcaaca ggcatttgtt
     8821 ttacccctga aggacaatat gccatcagtg gagatgacca cagctgaagc ggaaaatctg
     8881 atagccgcat tgcgctctgc tacggttagc gaagccattg gtgggcatag tcagattaca
     8941 ctgattttgg taccggatat ggctcggctt aatgacagtg atattgatga ctcctcaacc
     9001 caggtaagcc tgtggtccca aggctgggag gcgctgctgc aattgagtca ggttaggccc
     9061 aacctctttg tgctgttaga tgcgccggat aatgttgaac aggcgcagaa gtgtatgaca
     9121 acgctatcgt cagattatcg tcaatggggg gcagcatatt ggcctcgtct ggaaactacc
     9181 tatcagaaag aaatatctgg caaggacaat gaatctcagg gaattttcca ggggactgtt
     9241 ctgtcaccca cagccgcggt cgcagcggta attcaacgca cggataacga cgcgggtgtt
     9301 tggaaagcac cggccaatat tgccttatcc caggttattc gacctgttaa atcttatctt
     9361 cagggaagtg tactgtttaa cagcagcggc acttcgctca atgtgatccg cagtttccca
     9421 ggtaagggca tacgggtatg gggatgccgc actctggaaa acacggataa tacgcagtgg
     9481 cgctatctgc aaacacgtcg gctggtttcc tatgtaacag cgcatttgac ccaattggct
     9541 cgcatgtatg tcttcgagcc aaataatgaa cttacctgga tgaagttaaa aggacaaagt
     9601 tacaactggt tacggcaatt atggttgcag ggtggcttgt atggttcaca ggaggatgag
     9661 gcatttaaca ttctgttagg cgtaaacgag acgatgactg aggatgatgt tcgtgcagga
     9721 aaaatgatca tgaaagttga gttggctgtg ttgtttcctg ccgaatttat tgagatcagt
     9781 ttggtgttta atacccaaac agaggcgctg tcttaagaag gaaaaagtac gatgaacgat
     9841 tattacacac ccgtggtatc ccatcgtttt atggcgagtt ttatttttaa ccgcattccc
     9901 gatccgctgg atattcgttt tcagcgtatc tctggcctta gtcgggaact acaggtgact
     9961 cagtacagtg agggaggaga aaatgcccgt aataactatt tagctgagaa aatccaacac
    10021 ggtacgttga ctttggaacg gggcgtgatg acagtctcgc cattgacctg gatgtttgat
    10081 cgggtattga gtggtgaaaa aatcgcttat gccgatgtgg tggtgatgct actgaatgaa
    10141 aattcactgc cattgtccag ttggacgttg agcaatgcgc tgccggtacg ctggcaaacc
    10201 agcgactttg acgctaacag caatgccata ttggtgaata cccttgaatt gcgttaccag
    10261 gatatgcgct ggcttggagt caaaatatga cagtagaaat cagagagtta cttatccagg
    10321 caaaggtagt gccatcaaca cgaccgactg aatcagaacg gcaaaaccat tctttgatac
    10381 aggaaagtct ggatgaggcg acttgggtgg aaacgataaa acgcgaagtg ttggccgcat
    10441 tacgcgatga ggaagggtgg cgtccatgag tctgattgaa cgtggtttag ctaagctgac
    10501 aattaatgct tataaggata gggaagggaa gatacgggca ggaacgttgc aggccatgta
    10561 taaccctgac tccttgcaac tggattacca aacggattat cagcaatccc aagcgattaa
    10621 tagcgaaaag caaagtagca tttatgtaca ggccaagccc gcagggttat cacttgaatt
    10681 aatttttgat gccacgatgc cgggtaacaa aacccccatt gaagagcagc tcatgcagct
    10741 caagcaactg tgcagtgtgg atgcaaccag taacgagacg cgattcctgc aagttaaatg
    10801 gggcaaaatg cgttgggaaa gtcggggtta ctttgctggc agggccaaga gtttgtctgt
    10861 gaattacact ttgtttgatc gtgatgcgac tcccttgagg gtacgggtaa tattggcatt
    10921 agtggctgat gaaagtctgg tgttgcagga gactgaacaa aatctgcaat ctccggcaaa
    10981 aatcgcatta cgcatacagg atggggtatc tctggctctg atggcagcca gtacggcatc
    11041 aacattgtca ggcggtgtgg attatctgac gctggcctgg caaaacggtc tggataatct
    11101 caatgggttc gttccgggtg aaatattgca ggccaccagg ggagacgaat catgagccac
    11161 caactgaaaa ttattgcaga tggtaaggca ctgtcacttt tggccgcggt agatgtggac
    11221 acctgttatc gggttaacag tataccttct gcgacattga aactgagcgt accggatagg
    11281 ccactctctt ctttcagtca gacggatgtt cagacagaac tggcccactg tcaggtaggg
    11341 aaaaccctgc gtctggaatt gattgatggt agcaaaaaat gggtgctgtt taatggtctt
    11401 attacccgta aggctctgag aattaagaat aagcaattat tgctcactct ggttgtcaag
    11461 catcggttgc aactgatggt ggatacccag cattcacagc tgtttaaaga caaaagcgaa
    11521 aaagcgatct taagcacgct attgaatcag accggaatca atgctcgctt cggaaagata
    11581 gcggcgttag atcaaaagca tgaacagatg gtgcaatttc gttgttcaga ctggcatttt
    11641 ctgttgtgcc gactgtcggc aaccggtgca tggttgttac ctgccataga agacgttcag
    11701 tttgttcaac ctgatgctct gaaatcaaac tcagcctata ccttgaagag caggggggat
    11761 gagaacaaag acatcgttgt caaggatgct tactggcagt ttgacaatca aatcaacccc
    11821 gctttgctgg aagtcagtgg ctgggatatc agtaagcagc aggtacaatc aggcggtcgc
    11881 tacggaaaaa tcgcgttggg taaggcggca ctctctcctg atggattggc atcccttaat
    11941 aaaacgggtt gggacatttg ttatagcagt ccgttaacaa cccaggaaag cggttatctg
    12001 gcacagggat tattgcttaa ccagcgcatt tctggggtga caggagaatt tttgctcaaa
    12061 ggagatgggc gttaccagtt gggagacaac attcagctga ctggatttgg ttcacagtta
    12121 gatggtacgg caagcattac tgaggttcgc caccgtctta atcggcgaat tgattgggaa
    12181 accacggtga gcattggttt acaacatgaa tatttgccga tattacctga tgctcccgaa
    12241 ctacatattg cgacagtagc gaaatatcag caggacagtg cggtgttaaa ccgtatcccc
    12301 attattctgc cggtactgaa tcgtcccaat gaatttttgt gggccagatt ggggaaacct
    12361 tatgctagcc atgaaagcgg tttctgtttt tacccagagc caggtgacga agttattatt
    12421 ggtttttttg aaaatgatcc gcgttatcca gttattttag gtgctatgca taatccgaaa
    12481 aataaggccc cttttgaacc aacccaagat aatagggaaa aagtattgat cgttaaaaaa
    12541 ggtgaagcgc aacaacaatt agtcattgat ggcaaagaga aaatgatccg aattaatgcg
    12601 ggtgaaaatc aaataatgct tcagcaagat aaagacattt ctctgtcaac gaaaaaagaa
    12661 ttaacactga aagcgcagac aatgaatgcc acgatggata aatcattggc aatgtccggg
    12721 aaaaacagtg ttgaaatcaa aggcgcaaaa attaatctta cccaatgaaa ggtgacgatg
    12781 aatggaaaat caaatactga cacaactcta tggtcgtggt tgggcttttc ctccggtctt
    12841 ttcccttgaa aagggggtag agatggctga aggggcggaa gatgtgagac aaagtttgca
    12901 gattctgttt agtactgagc cgggggaacg tcttatgcgt gaaaattatg gctgcggatt
    12961 aaatgatttt atgtttgaaa atatccgcaa tgaacttatt gctgaaattg aatcccatat
    13021 ccatgacaac gtattacgat atgaaccccg ggctgatatg actgatattc aggttcgtca
    13081 atcccctggc atggggaata ctttgcaagt gcaggtcatg tatcgcctga gagggagtga
    13141 tatcaatcaa caaatccagg gagtacttgc actgagtgaa ggccgggtga cggaggtagt
    13201 atgagtgcac cgaaaaagaa acgcaaagtt ggtattcatg gtgttccggc agcaatggat
    13261 aagaaatact caataggctt agatatcggc acaaatagcg tcggatgggc ggtgatcact
    13321 gatgaatata aggttccgtc taaaaagttc aaggttctgg gaaatacaga ccgccacagt
    13381 atcaaaaaaa atcttatagg ggctctttta tttgacagtg gagagacagc ggaagcgact
    13441 cgtctcaaac ggacagctcg tagaaggtat acacgtcgga agaatcgtat ttgttatcta
    13501 caggagattt tttcaaatga gatggcgaaa gtagatgata gtttctttca tcgacttgaa
    13561 gagtcttttt tggtggaaga agacaagaag catgaacgtc atcctatttt tggaaatata
    13621 gtagatgaag ttgcttatca tgagaaatat ccaactatct atcatctgcg aaaaaaattg
    13681 gtagattcta ctgataaagc ggatttgcgc ttaatctatt tggccttagc gcatatgatt
    13741 aagtttcgtg gtcatttttt gattgaggga gatttaaatc ctgataatag tgatgtggac
    13801 aaactattta tccagttggt acaaacctac aatcaattat ttgaagaaaa ccctattaac
    13861 gcaagtggag tagatgctaa agcgattctt tctgcacgat tgagtaaatc aagacgatta
    13921 gaaaatctca ttgctcagct ccccggtgag aagaaaaatg gcttatttgg gaatctcatt
    13981 gctttgtcat tgggtttgac ccctaatttt aaatcaaatt ttgatttggc agaagatgct
    14041 aaattacagc tttcaaaaga tacttacgat gatgatttag ataatttatt ggcgcaaatt
    14101 ggagatcaat atgctgattt gtttttggca gctaagaatt tatcagatgc tattttactt
    14161 tcagatatcc taagagtaaa tactgaaata actaaggctc ccctatcagc ttcaatgatt
    14221 aaacgctacg atgaacatca tcaagacttg actcttttaa aagctttagt tcgacaacaa
    14281 cttccagaaa agtataaaga aatctttttt gatcaatcaa aaaacggata tgcaggttat
    14341 attgatgggg gagctagcca agaagaattt tataaattta tcaaaccaat tttagaaaaa
    14401 atggatggta ctgaggaatt attggtgaaa ctaaatcgtg aagatttgct gcgcaagcaa
    14461 cggacctttg acaacggctc tattccccat caaattcact tgggtgagct gcatgctatt
    14521 ttgagaagac aagaagactt ttatccattt ttaaaagaca atcgtgagaa gattgaaaaa
    14581 atcttgactt ttcgaattcc ttattatgtt ggtccattgg cgcgtggcaa tagtcgtttt
    14641 gcatggatga ctcggaagtc tgaagaaaca attaccccat ggaattttga agaagttgtc
    14701 gataaaggtg cttcagctca atcatttatt gaacgcatga caaactttga taaaaatctt
    14761 ccaaatgaaa aagtactacc aaaacatagt ttgctttatg agtattttac ggtttataac
    14821 gaattgacaa aggtcaaata tgttactgaa ggaatgcgaa aaccagcatt tctttcaggt
    14881 gaacagaaga aagccattgt tgatttactc ttcaaaacaa atcgaaaagt aaccgttaag
    14941 caattaaaag aagattattt caaaaaaata gaatgttttg atagtgttga aatttcagga
    15001 gttgaagata gatttaatgc ttcattaggt acctaccatg atttgctaaa aattattaaa
    15061 gataaagatt ttttggataa tgaagaaaat gaagatatct tagaggatat tgttttaaca
    15121 ttgaccttat ttgaagatag ggagatgatt gaggaaagac ttaaaacata tgctcacctc
    15181 tttgatgata aggtgatgaa acagcttaaa cgtcgccgtt atactggttg gggacgtttg
    15241 tctcgaaaat tgattaatgg tattagggat aagcaatctg gcaaaacaat attagatttt
    15301 ttgaaatcag atggttttgc caatcgcaat tttatgcagc tgatccatga tgatagtttg
    15361 acatttaaag aagacattca aaaagcacaa gtgtctggac aaggcgatag tttacatgaa
    15421 catattgcaa atttagctgg tagccctgct attaaaaaag gtattttaca gactgtaaaa
    15481 gttgttgatg aattggtcaa agtaatgggg cggcataagc cagaaaatat cgttattgaa
    15541 atggcacgtg aaaatcagac aactcaaaag ggccagaaaa attcgcgaga gcgtatgaaa
    15601 cgaatcgaag aaggtatcaa agaattagga agtcagattc ttaaagagca tcctgttgaa
    15661 aatactcaat tgcaaaatga aaagctctat ctctattatc tccaaaatgg aagagacatg
    15721 tatgtggacc aagaattaga tattaatcgt ttaagtgatt atgatgtcga tcacattgtt
    15781 ccacaaagtt tccttaaaga cgattcaata gacaataagg tcttaacgcg ttctgataaa
    15841 aatcgtggta aatcggataa cgttccaagt gaagaagtag tcaaaaagat gaaaaactat
    15901 tggagacaac ttctaaacgc caagttaatc actcaacgta agtttgataa tttaacgaaa
    15961 gctgaacgtg gaggtttgag tgaacttgat aaagctggtt ttatcaaacg ccaattggtt
    16021 gaaactcgcc aaatcactaa gcatgtggca caaattttgg atagtcgcat gaatactaaa
    16081 tacgatgaaa atgataaact tattcgagag gttaaagtga ttaccttaaa atctaaatta
    16141 gtttctgact tccgaaaaga tttccaattc tataaagtac gtgagattaa caattaccat
    16201 catgcccatg atgcgtatct aaatgccgtc gttggaactg ctttgattaa gaaatatcca
    16261 aaacttgaat cggagtttgt ctatggtgat tataaagttt atgatgttcg taaaatgatt
    16321 gctaagtctg agcaagaaat aggcaaagca accgcaaaat atttctttta ctctaatatc
    16381 atgaacttct tcaaaacaga aattacactt gcaaatggag agattcgcaa acgccctcta
    16441 atcgaaacta atggggaaac tggagaaatt gtctgggata aagggcgaga ttttgccaca
    16501 gtgcgcaaag tattgtccat gccccaagtc aatattgtca agaaaacaga agtacagaca
    16561 ggcggattct ccaaggagtc aattttacca aaaagaaatt cggacaagct tattgctcgt
    16621 aaaaaagact gggatccaaa aaaatatggt ggttttgata gtccaacggt agcttattca
    16681 gtcctagtgg ttgctaaggt ggaaaaaggg aaatcgaaga agttaaaatc cgttaaagag
    16741 ttactaggga tcacaattat ggaaagaagt tcctttgaaa aaaatccgat tgacttttta
    16801 gaagctaaag gatataagga agttaaaaaa gacttaatca ttaaactacc taaatatagt
    16861 ctttttgagt tagaaaacgg tcgtaaacgg atgctggcta gtgccggaga attacaaaaa
    16921 ggaaatgagc tggctctgcc aagcaaatat gtgaattttt tatatttagc tagtcattat
    16981 gaaaagttga agggtagtcc agaagataac gaacaaaaac aattgtttgt ggagcagcat
    17041 aagcattatt tagatgagat tattgagcaa atcagtgaat tttctaagcg tgttatttta
    17101 gcagatgcca atttagataa agttcttagt gcatataaca aacatagaga caaaccaata
    17161 cgtgaacaag cagaaaatat tattcattta tttacgttga cgaatcttgg agctcccgct
    17221 gcttttaaat attttgatac aacaattgat cgtaaacgat atacgtctac aaaagaagtt
    17281 ttagatgcca ctcttatcca tcaatccatc actggtcttt atgaaacacg cattgatttg
    17341 agtcagctag gaggtgacaa acgtccggca gcaaccaaaa aggcaggtca ggccaaaaag
    17401 aaaggtggtt caggtggtgg tggtagcggt ggtggcggtt ctggcggtgg cggtagtggc
    17461 ggaggtggaa gcggaggcgg tggcagtggt ggtatgagtg aagcgattgt ggtggatggt
    17521 gacgtgttac agtttgatcc caactttggc aatcggcagg tgacggttcc cagcccagga
    17581 aaaattagcg gcacaggaca tgcgcaggta agtggaaaaa aagtgtgtat tctgggggat
    17641 gagaaacagg tcagggtttc tgcaacctat attacaacaa cacatactac gccgggaaca
    17701 ggaaccatta ctatcagtgc tctggatgct ggccagcagg cccttcagtg taccagtggg
    17761 gcggctttaa ttatcaaggg gcagcaattt acggcgatgt ttacgcctga attgccagcc
    17821 atgaataata cagtgactcc gccacaaccg gatgttacga caccttcatc aggaaaagga
    17881 cgttttatca ctcaacaaaa ttttgctacc gtaaattaga gtattgactg aattaaatag
    17941 aattaacgaa ggtgtaaata attatttatt tgctgacgaa tcgctgtgac aaataaacac
    18001 aggtgatgtt atggaattaa atgagttaac taacaaattg tcaaatttgg tgccaatgac
    18061 cgattttaaa ttagataatc gagccagttt gcaattgctt aaatatattg aagcgtatac
    18121 gaagataata ccctttaatt ctggcgataa atattggaat gactttttct ttatgtcagg
    18181 aaatacgcca gagaaacttg caaaattata tcagaaagaa atagaaccca atggggagtt
    18241 attacctcag caggcttttt tgttggcggt tttgcgttta ttggaaacac caatatcctt
    18301 attaaatgta ttacctgctg ctcatcgtga gctctattat cgggagcttt taggcttgtc
    18361 ttcccatgcg gcacagcctg atcaggttgc tttatctatg gaactgaatt cgacagtgat
    18421 ggaacagctg ctccctgaag gaaccctgtt tgaggctggt caggatgaac aaggcaatgc
    18481 attgcaatat gccctggatg ccagtttgct ggctaatcgt ggatatatca gtgacttgcg
    18541 ctggttacgg aatgacgggg aaaagcaatg ggttacttct gctccatggg atttacaggc
    18601 acaggtgtca ctgccgtctg atgggatacg attatttggt aagacaaata gtgatcagca
    18661 ggtatttggt ggggtgttga taacgtcatc acttctggcg atggaagcgg ggataaggaa
    18721 gatcattgtt acttttgagc aggagatgaa cacccaagaa ctggtggcac aggtcagcag
    18781 tggaaatcaa tggctaacat tgacgtctga ggtaaataag aaagaggtca cactgacact
    18841 gtcagacaaa gaaccggcaa tcagtgcgcc agaggatctg gataatctct ttttcacgca
    18901 accggtactc aggctacagg gaaaggatag tcaggcactg ccggaggtga cgggtatcag
    18961 cgtttcggaa aaggatgata ctaaggatac ctcttttgag atgtatcact taacaccatt
    19021 tggttatagc agtgatatag agccattgga ggaaaatcca gcgttatatt taggctttac
    19081 tgatgtaaag ccagggcaaa cactggcgct gtattggaaa ttaaaatccc cgcagcaacc
    19141 aaccgtttcc tggtattacc tggatcaaca taatcaatgg gctgaattgg attcatgggt
    19201 cagtgatgga acccagaatc tgtatcagga tggtacttgg cacgttgagt tgcctgtgga
    19261 tgcatccaat caggcagagc agatgccagt tggacgctat tggttgcggg cagtggtgga
    19321 ggtacccgct catgaggggg cgttggggaa ggctccttgg ctatatggtc taatctataa
    19381 cgccatgacg gcaaccttgg ttaatgtaga tagcatcagt gacagccatt tcttaacccc
    19441 tttgcctgcc agcagcatac agcggcccgt tgaacccatc attgtgttgg catcggtcaa
    19501 ccagccttgg gcatcatggg gtggacgtat acctgaatcc tacagtgcct tttttgaacg
    19561 gatagctcaa aacctgtctc atcgaaaccg gtccttaacc tggggaaata tggtgacatt
    19621 actcaaagag cgttatgtca gcatctttga tgttaagtat ccaggtaatg atgaactcac
    19681 cagagtgcca gcattggagc agcagcaact aacagtgatt ccagcaaacc ggtacaacga
    19741 tagcgatgat tctctgcgtc cggtactgaa tcctgctcgt ctgcaagaga tggctgattg
    19801 gttgcagcag aaagactctc cctgggcctc tattgaggtc aggaatccag aatacttgga
    19861 tgtgaaaatc cattacgagg tgatttttaa acctgatgtg aacgaagatt ttggctatcg
    19921 ccagctacag cagcaactgt gtgaggtgta tatgccttgg agcatagatg agcagcggcc
    19981 cgttgtattg aataacagca ttaattattt ccagttgtta gccactattc aacagcaacc
    20041 gctggttgag cgagtcactc gtctgacact acatcgggct gattcttctg atgagagtga
    20101 tggtacagca tctgtggaag ccaaagataa tgaagtgctt attttagtct gggaagagga
    20161 cgataatctg caataccgag gaaatgacta tgagtaatca ggatgcactg tttcatagcg
    20221 ttaaagacga tattcacttt gataccttgc tggaacaagc tcatcaggtg attgaaaaac
    20281 aggctgaaaa actgtggagt gatacggcag agcatgatcc gggtatcaca tttttgcagg
    20341 gaatcagtta cggtgtgtca gatttggctt accgacatac attacccctg aaagatttac
    20401 tgactccggc gccggatgag cagcagcaag agggaatttt tcctgccgaa tttggcccgc
    20461 ataatacact gacttgtggg ccggtgacag cggatgatta tcgcaaggca ttgttagatc
    20521 tacacagcag cgacagcctg gatggtactc agcaggatga gggggatttt ctgttccgga
    20581 gtgtgcaact ggtgcgtgaa ccggaaaaac agcgttatac ctattggtat gatgcaacca
    20641 agagggaata tagctttgtc aacagtgaag gggctaaaga gtttaccttg cgggggaatt
    20701 actggttgta tctggaacca acccgttgga ctcagggtaa tattgccgct gctaccagac
    20761 aactgacaga atttttgact aaaaatcgca atattggtga atctgtcagc aacattatct
    20821 ggctacaacc ggttgatctg ccactgttgc tggatgttga actggatgat gatgtaggtg
    20881 cacaggatgt ccccggtatt tttgcggcgg tgtatagcac cgcagagcag tatctgatgc
    20941 ctggagcaca gcgttaccgt acggaagtac tgcaaaatgc tgggatgagc aatgatcaaa
    21001 tcttcgaagg tccattattg gaacatggct ggataccaga gctgccggca gcccgtgatt
    21061 atactcaaag gctcactctc aatcttagcc ggttggtaaa tagtctgctt gagattgagg
    21121 gcattaaaca tgtgaatcgt cttcgtctgg atgatagctt cgataaaact gctattgaac
    21181 ccgttaaggg ggatacctgg tcgtggtcga tcaaagaggg ctattatcca cgtctttggg
    21241 gagaagaccc acttaaccaa ttggcgcaac aaaatggccc gcttagggtg atagccaaag
    21301 gagggattag cgtcagtgtg agtaaagagc aaatccaggc cagtttaccc agtcaatcac
    21361 tgattcaaaa tgagccggta atattggctt acggccagca ccgtgacgtt ggcagctatt
    21421 atcccgtcag tgatactttg ccgccttgct atggactaca acattctttg tctgaaagtg
    21481 aacacttatt gccacttcat caatttatgt tgccatttga acaattattg gcctgtggtt
    21541 gtcaacagat agccatgctc ccgcggttac tggcttttca gcgcgaaggt tatgaggttt
    21601 ggggtgatca gtggcccttt aagtcaggct cagtgaatga tgacgcccat caagattatg
    21661 cccctgcatt aaaggatttg ttaggacaga ttgcgctgga tagtgatcat gaattggata
    21721 ttattaatta cttgctgggt tactttggca cacagcgggc accgcgtacc tttacgacac
    21781 aactcgatga ttttcgtgcg gtccaacagg gttatctggc ccagcaaccg acattgactt
    21841 accaccgctc caatattcgt atcgatcagg tatcgtcgct acaaaaacgt attgctgctc
    21901 gcatggggct gggcggtgag ttgtttaaac ctcaaccgga tctgagccaa ctgccttttt
    21961 atttgattga acatcgagcg ttgctgccag tcaaacccaa tagtcagttt gataaggaac
    22021 agaaaccagc ctcggtgaca gaggaggggg gcagccaaac aggtcaacat tatgtggtca
    22081 ttgaacagaa gggcattgat ggcaagctga cacaggggca agtgatcaat ttaattctgt
    22141 atgaaggaga gcagggagaa acccaattta cgatacgcgg tcagatggta ttcaaaaccg
    22201 agggggataa gttttggttg gatgtgaata atagtgcgca actggaatat aatctggcgc
    22261 gggtaatgac agcagccaag gcgagtaaac tcttttggca aaacagcccg gtatggatgg
    22321 aggatatggg ctatcgtctg gcctatgcta gtgaccaatc ctcattgcct gtgaatcaac
    22381 ggcgcttgac ccgcacagtg caaactccat tcccgccgat ggttgttgta ggtagcgaaa
    22441 tcaccctgtt aaagcaggtg gggatagtca atttaaaaaa agcggagtca gaaaaacttt
    22501 atgcaaaagt tgttagcttt gatcgcattg aagggacctt gattattgag cgtttgggta
    22561 attccactct ggcttttcct acctcggaag aggcgtggcg gtatagttgg tatttttcgg
    22621 gggagaaata tgaaaggact gaccgctttt catttgtgat tagcgtagta gtgaacagtg
    22681 acttaattaa attgcccggt gttgatccct ataaattgga agaatgggtg aaagaaacga
    22741 ttcttaccga atttccagct catatttcta tgattatcca ttggatggat cgggaagcct
    22801 ttttaaattt cgccaatacc tatcagcgtt ggcaaaataa tggtacgcca ctgggggatg
    22861 cggcttattc cattctagaa agtttgacac ttggtaaatt gccatctgcc ttaaaaggtg
    22921 ttggcacaat gcgtattgcc acatctagtc aaagagaaga agtggtgggt agtaatggtg
    22981 atcaatggaa tacagatgga ataacccaga atgaattatt ctatgttcct aaagagagct
    23041 aggaaaaata aatatctgcc actaatgatg ttgaattaaa tatgttttct ggagttaatc
    23101 atgaacgaaa ctcgttataa tgcaactgta caagaacaac aaacattatc taatccaaaa
    23161 gctgttggac ctgacatcga taaattaaag gataaattta aagagggcag tattcccctg
    23221 caaaccgatt tcaatgagtt aattgatatt gccgatattg gacgtaaagc ctgtggtcaa
    23281 gcgccacaac aaaatggccc aggagaagga ttgaaattgg ctgatgacgg tacgcttaat
    23341 ttaaaaatag gcactttttc caataaagac ttttctccat taatattaaa agatgatgtt
    23401 ttatctgtag atcttggtag tggtctgact aatgaaacca atggaatctg tgtcggtcag
    23461 ggcgatggta ttacagttaa cactagcaat gtagctgtaa aacaaggtaa cggaattagc
    23521 gttactagta gtggtggtgt tgccgttaaa gttagtgcta ataagggact tagcgttgat
    23581 agtagtggtg ttgcagttaa agttaatact gataagggaa ttagcgttga tggtaatggt
    23641 gttgcagtta aagttaatac tagtaaagga attagcgttg ataatacagg tgttgcagtt
    23701 atagctaatg ctagtaaggg aattagcgtt gatggtagtg gtgttgcagt tatagctaat
    23761 actagtaaag gaattagcgt tgatggtagt ggtgttgcag ttatagctaa tactagtaaa
    23821 ggaattagcg ttgataatac aggtgttgca gttatagcta atgctagtaa gggaattagc
    23881 gttgatggta gtggtgttgc agttatagct aatactagta aaggaattag cgttgatggt
    23941 agtggtgttg cagttatagc taatactagt aaaggaatta gcgttgatag tagtggtgtt
    24001 gcagttaaag ttaaagctaa tggcggaatt aaagtagatg ctaatggtgt tgcaattgat
    24061 cctaataatg tactccccaa gggagtgatt gtaatgttct ctggcagtac tgcaccaact
    24121 ggttgggcgt tatgtgatgg caataatggt acaccaaatt taatcgatcg atttatttta
    24181 ggtgggaaag ggactgatat taatggagtg agtactaata cagcttcagg tactaaaaat
    24241 agtaagttat tcgatttcag ttctgatgaa gctacattaa ctattgatgg taaaacactg
    24301 gggagaggcg gttctggcgg cggtggttct ggcggtaacg ataaactgac cctgtggacc
    24361 acaccggcac cgagtccgaa ttgtcgtctg aatgcagaaa aagatgcaaa actgacactg
    24421 gttctgacca aatgtggtag ccagattctg gcaaccgtta gcgttctggc agttaaaggt
    24481 agcctggcac cgattagcgg caccgttcag agcgcacatc tgattattcg ttttgatgaa
    24541 aatggcgtgc tgctgaataa tagctttctg gatccggaat attggaattt tcgtaatggt
    24601 gatctgaccg aaggcaccgc atataccaat gcagttggtt ttatgccgaa tctgagcgca
    24661 tatccgaaaa gccatggtaa aaccgcaaaa agcaatattg ttagccaggt gtatctgaac
    24721 ggcgataaaa caaaaccggt tacactgacc attacactga atggcaccca agaaaccggt
    24781 gataccacac cgagcgccta tagcatgagc tttagctggg attggagcgg tcataactat
    24841 atcaatgaaa tttttgcgac cagcagctac acctttagct atattgcaca agaaggtggc
    24901 tctggtggtg gtggttcagg tggtcacgat catgatatta agataacggg cacaggaaaa
    24961 cattctcaca aaaacaaagt aacagtccct tattatattc tggctttcat cataaagctt
    25021 taatatatat gaaaaattga aaatataaat tatccattaa taataaagag gatattagca
    25081 tgacttcgga gccaaatctg ttaaaccgga ttacaattac tattgaagct aataatcaac
    25141 aagtagctag aaaagtattg catggctcct tgcttaatca agctaatata aataaattat
    25201 ttaattcata ctttaatgaa tatgaaatta ataggggtgt ttatttagaa acattaatcc
    25261 tgaatcttgg tacgataaat ttccatgatt ttaattcatt gtttcctact ctcctaaaag
    25321 ctgcattgaa taaagaattc agtcaatatc agataaacaa ccatagggaa gaaatgctat
    25381 ttaatgagac aatatcaaat caagctactg ataagtctta catatttggc gataacaaat
    25441 taattgatgc agagaatttc attcactttt tatatcaaaa gcattccaca ttaaatctag
    25501 tagaagcaat gggaaataat ggtattgaaa aattaacaaa tcagttaaca caaatagaaa
    25561 ataaatttgc gttattattg gcaaaaagtt gtttgtctga ggaaggctta aaacgactct
    25621 tggctatcaa acaacccgat ttattaatcg ctatcaatcg cagattatct gaaagaataa
    25681 atagaccaca atatcaggag aagcttgttt cctgcggaca actgatattt agtgctctgg
    25741 gatatataca acagtacaat atacaggaaa ttcctaaacc ggatgaaaaa gttattgcac
    25801 gcataacaac tgaacttaat aataatggtt tgcttaatac aatacctatt attacactat
    25861 ttcgtcagag tgggattaac gattcatcac taaatgattg gctaaagaaa atctggcagg
    25921 tgagatcaat ttcacagtta tgcagaaagt atctttctgc taaggaatac caatatctgt
    25981 cagaacattt tgtttcaaag agcgtcgata aaaatagata tgatgaagag cccgtaaatc
    26041 agagcatatt atcaaggttg aataataatt ccattaaaga aggaaataat cacagtcaac
    26101 tctgtactct cagtagacta tattctgaac ccgttgtatt acctgaacaa accattctac
    26161 gtcaggttag taatacagta gatcagagca tattatcaag gttgaataat gcctccatta
    26221 aagaaggaaa taaccaaagt caacttcgca ctctcagtag actatattct gagcccgttg
    26281 cattacctga acaaaccatt ccacgtcagg ttagtaatac aggtatatta attctatggc
    26341 caatgctacc tacactattt aaccagcttg gtctacttga gaaaaagaaa tttatccatc
    26401 gtcaggccca gtttaatgcc gttgattttc ttgattacct gatttgggga accgaagatg
    26461 tgaaagtgga acgaaaggtt ttgaataatg ttctatgtgg gttaatggct gatgaaatta
    26521 ctgaaccaat gcctattgaa ccagaaaaac aatggataat aattcaatgg ctggacgcta
    26581 ttatctccca actttctggc tggaaaaagt taagtcgtaa tgacgtccgt caattatttc
    26641 tacaacgacc aggagaatta ctgatcaatg aacaggaaat taaaatcaca atacagcaac
    26701 aaccatttga tgctctgtta actgattggc cgtggccaat gaatatggct tgttttagct
    26761 ggttgagtca accattaacc attacgtggt tataaccatt gaccacaatg acttagtctg
    26821 agtaaaaaat atgaatatat cgcctgtttt ttatgattca ttgaatcagg ataacgaccg
    26881 tgatctatcg tttttattta gcgaactgga acgaatagat ctcgctcttc aacaccattt
    26941 ttattgtgta gaaagtcagc gaagtgagct cctggatgag tttctgctca ctgaggcgga
    27001 agtggtgacc aggctggata agccacttgg taaacctcat tggataaatg atgattatct
    27061 ggcgatatcg caaaagggca atgtaagcct aatggcagcg tccagattaa tggatctgat
    27121 cgaacgcttt gaactgactg attttgagcg cgatgtttta ctattaggct tattgcccca
    27181 ttttgatagc cgctattatc gactgttttc gctgattcaa gggggacaac agggtcgatt
    27241 accttctttt gcgctggcat tggaactgtt ttgccactcg gcgctggaga aacaggtaca
    27301 gcaagcgagt tttctgcacc gggcaccttt gatgggttgc cagctattat ccatcgatac
    27361 tagtcaaaaa acgctggcct ggctccagac tccctttatt actgacagcg gggtatatca
    27421 ctttttactg gggcatcact acattatgcc ggctttagaa cattgtgctg agtggttaac
    27481 accgacaggg attggctgtt atcctgaagg attaaaacaa gtactgggta acgtattgtt
    27541 atctgacaac gataatatta gaccgattgt cttattacgg ggaatggccg gcagtgccag
    27601 agcttatacc attactaata tgatggcttc agaagggaag caaacactgc tggtagatat
    27661 atccaaactt gctgatagcg atgaaaaaaa cattattctt cagataaagc atattttgcg
    27721 ggaaacccgc atgcatggag catgtttatt attacggaat ttttgcttgt tagtggaaca
    27781 gaataaacaa ctattggact ccctgtcaga gttattgaat caacctgaat taagaattgt
    27841 ttgcctgatt gagccttatt ccccattggt atggctgaaa aagataccgg tattactgat
    27901 tgagatgcca cttttaacgc ctgcggaaaa agccagattg ttaattgcca gcttaccgga
    27961 taattgttcc gaggatattg atacgataac tttaagccag cgttacactt ttaacccaga
    28021 aaccctgcca ttgattttgc aagaggccca gctttatcaa cagcagcgag atccgctgga
    28081 tatcttgcag caatgcgata tacgccaggc attaaatttg cgtgctcaac aaaatttcgg
    28141 tcaattggca cagcggatta ttcctaagcg ctcattaaag gatttattgg tatccgatga
    28201 gattgctcag cagttacggg aaatactcat agcaattaag tatcgggaac aggttctggc
    28261 gggagggttt aaagataaaa ttgcctatgg cactggtatc agcgccctgt tttatggtga
    28321 ttcaggcact ggaaaaacca tggcagcaga agtgattgct gaccacattg gcgttgactt
    28381 aataaaagtg gatttatcta cagtagtgaa taaatacatc ggtgaaacag aaaaaaactt
    28441 atcccgtatt ttcgatttgg cggaacagga tgcaggggta ttattctttg atgaagctga
    28501 cgcactgttt ggtaaacgca gtgaaactaa agattcccag gacagacatg ccaatattga
    28561 agtttcttac ttattacagc gcctggagaa ttacccgggt ctggtcattt tatccaccaa
    28621 taatcgtggt catttagaca gtgcttttaa tcgtcgtttt actttcatta cccgttttac
    28681 ttacccggat gaaaaaatcc gtaaaaaaat gtggcaggaa atttggccta gaaatataaa
    28741 aatatcggaa gatatcgatt ttaacgaatt agctcaacga acaagcgtga ctggcgcgaa
    28801 tatccgcaat attgctttat tgtcttcatt ctttgcttca gagcagggga atgatgaagt
    28861 cagtaatgaa aatattgaaa ttgcattgaa gcgtgaatta gctaaagtcg gacgattaac
    28921 attttaaaag ttatcacaat gaaagtattg aaatattaaa taaatttatt accaaaaagt
    28981 tatcacgata taatttaaga gaggtttttt atgttaaaca cgcaaactat tattgatgtc
    29041 aataaggcaa tggatgccat gctgcgcgca tatctgaatc aagatattgc cattcgtttt
    29101 gatctacctg aattggatac tatgcaatct gatgcgatgg taagtatctt tctttatgac
    29161 attcatgaag atttacagct tcgctcggca gaatcaagag ggtttgatgt ttatgccggg
    29221 aggttattgc ctggttgggt aaatattaaa tgtaactatc tgattaccta ttgggaagct
    29281 tctaagccag cgactgatgc cagcagtccg gatagccaac ctgataacca ggcaatacaa
    29341 gtgatgtcac aagtattaaa tgccttgatt aataatcgtc aattggcagg tattcctggt
    29401 gcttatactc aggttgtacc gcctaaagag agtttaaata gcctggggaa tttctggcaa
    29461 tcactgggta atcgcccacg gctttctctc aattattcag tgacagtacc tgttagccta
    29521 aacgatggtc aggatagcgc gactccggtt accgcggttt cttctacagt ggaacaaacg
    29581 gcatcgctca gtcaagaagt ggttagtcat gctttacgcg aattactcat tacggaatta
    29641 ggaggaggag aggataaccg gttggtactg agtaaagttg aattatccgc agtgaaagag
    29701 acgatgactc aagacagtcc ggctcagatg attatattgt tgtctgtttc aggcattaca
    29761 cgacaggaat atttgaagga aattgataat atctttgatc gttgggtaaa taatgctgaa
    29821 gttattacca ctattgatga ttgtgggatt agaattgaaa gtataacgaa agataatctt
    29881 gtaggaattt aaattttttt aattaaataa aggaattaat attgatttga tagagcatgt
    29941 aatcaataac ttctatgaaa atgttgaaaa acaatacacg gaaacactta acttacaatt
    30001 aaattgttaa ag
//
