LOCUS       sequence-487021-        7638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER expression of green glucose sensor (high affinity) with 
            non-responsive HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7638)
  AUTHORS   Marvin JS, Machler P, Meng C, Ates T, Patel RH, Adhikari R, Makurath
            MA, Ku Z, Feliciano D, Atasoy D, Cui G, Kleinfeld D, Brown TA
  TITLE     iGlucoSnFR2: A genetically encoded fluorescent sensor for measuring 
            intracellular or extracellular glucose in vivo in mouse brain.
  JOURNAL   Sci Adv. 2025 Nov 14;11(46):eadz3889. doi: 10.1126/sciadv.adz3889. 
            Epub 2025 Nov 12.
  PUBMED    41223288
REFERENCE   2  (bases 1 to 7638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-11-14"; volume: "11(46):eadz3889. doi"; pages: " 
            10.1126/sciadv.adz3889. Epub 2025 Nov 12"
COMMENT     Sequence Label: pAAV.CAG.(ER).iGlucoSnFR2.H348H.HaloTag
FEATURES             Location/Qualifiers
     source          1..7638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   61..190
                     /label=AAV2 ITR
     rep_origin      complement(427..1015)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(507..526)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(1186..1977,1978..2046))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTXIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1809..1828
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(2047..2151)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(2177..2632)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2314..2335)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2526..2545
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2704..2833
                     /label=AAV2 ITR
     promoter        3146..3423
                     /label=chicken beta-actin promoter
     primer_bind     3702..3724
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     3786..3805
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     primer_bind     5089..5110
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(5213..5234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6785..7373
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6838..6858)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7256..7267)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    7416..7537
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7427..7446)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     7481..7500
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ggatcttcct agagcatggc tacgtagata agtagcatgg cgggttaatc attaactaca
       61 aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg ctcactgagg
      121 ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca gtgagcgagc
      181 gagcgcgcag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg
      241 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
      301 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
      361 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
      421 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
      481 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
      541 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
      601 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
      661 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
      721 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
      781 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
      841 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
      901 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
      961 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     1021 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     1081 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     1141 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     1201 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     1261 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     1321 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     1381 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     1441 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     1501 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     1561 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     1621 tcctccgatc rttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     1681 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     1741 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     1801 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     1861 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     1921 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     1981 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     2041 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     2101 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     2161 ccgaaaagtg ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
     2221 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca
     2281 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta
     2341 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta
     2401 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg
     2461 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga
     2521 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg
     2581 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg
     2641 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     2701 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
     2761 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
     2821 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
     2881 tctaggaaga tcgtaccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata
     2941 gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta
     3001 catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc
     3061 gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca gtacatctac
     3121 gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt cactctcccc
     3181 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
     3241 gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg gggcgagggg
     3301 cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc gctccgaaag
     3361 tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag cgcgcggcgg
     3421 gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc ctcgcgccgc
     3481 ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga cggcccttct
     3541 cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct gtggctgcgt
     3601 gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc ggggctgtcc
     3661 gcggggggac ggctgccttc gggggggacg gggcagggcg gggttcggct tctggcgtgt
     3721 gaccggcggc tctagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     3781 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tggatcccgc
     3841 caccatgctg ctatccgtgc cgctgctgct cggcctcctc ggcctggcca tcgccagatc
     3901 taagctggag attttctctt ggtgggctgg ggatgaaggc cctgccctgg aggcactcat
     3961 cagactgtat aaacagaagt atcctggagt agaggtgata aacgccactg tcaccggagg
     4021 tgccggtgta aatgctcgcg ctgtccttaa gacaaggatg cttggtggtg atcctcctga
     4081 tacatttcaa gttgcagcag ggatggagtt gataggcaca tgggtggtgg ctaacagaat
     4141 ggaggatctc agcgccttgt ttcgacaaga gggttggctt caagccttcc ccaagggcct
     4201 tattgatctg attagttata aaggtggtat atggagtgtg cccgttaata ttcacaggtc
     4261 aaacgtaatg tggtatttgc ctgcaaagct aaaagagtgg ggagttaacc ccccacggac
     4321 ttgggatgag tttcttgcca cctgtcaaac acttaaacaa aagggtctgg aagctccact
     4381 ggctctcggg gaaaattgga cacaacaaca cctctgggag agtgtcgcct tggctgtatt
     4441 ggggcctgat gattggaata atctgtggaa tgggaagctg aagttcacag accctaaagc
     4501 agttagggca tgggaagtct ttggccgcgt tttggactgt gccaacaagg atgcagcagg
     4561 cttgtcttgg cagcaagctg tcgatcgggt agtacagggg aaagccgctt ttaacgtaat
     4621 gggcgactgg gcagcaggat atatgactac cactctcaag ttgaagcctg gtacagattt
     4681 cgcatgggca ccaagtcccg ggacacaggg cgtctttatg atggtttccg actctttcgg
     4741 acttcccaaa ggagccaaaa atcgacaaaa cgctatcaac tggcttcgat tggtcggctc
     4801 taaagaaggt caagatacat tcaacccact gaaagggtcc atcgctgctc ggctcgatag
     4861 cgacccaagc aagtatccgt gtaacgtcta tatcaccgcc gacaagcaga agaacggcat
     4921 caaggcgaac ttcaagatcc gccacaacgt ggaggacggc agcgtgcagc tcgccgacca
     4981 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     5041 ggtgacccag tccgtgctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     5101 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtac
     5161 cggagggagc atgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     5221 gctggacggc gacgtaaacg gccacaagtt cagcgtgcgc ggcgagggcg agggcgatgc
     5281 caccaacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     5341 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     5401 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     5461 catcagcttc aaggacgacg gcacctacaa gacccgcgcc gaggtgaagt tcgagggcga
     5521 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     5581 ggggcacaag ctggagtaca actttaacaa ccccaacgca tacggacaga gtgcaatgcg
     5641 agattggcgg agtaatcgca tcgttggctc cctggtacac ggagcagtgg cccctgaaag
     5701 ctccatgtca caattcggaa cagtaatgga aattttcctg caaacacgca acccacaagc
     5761 agcagcaaat gcagcacaag ctattgctga ccaagttggc ctgggtcgcc ttggtcagct
     5821 gcagggcgga ggctcgggcg gaggtgggtc gggtggcggc ggatcaatgg ccgagatcgg
     5881 caccggcttc cccttcgacc cccactacgt ggaggtgctg ggcgagcgca tgcactacgt
     5941 tgacgtgggc ccccgcgacg gcacccccgt gctgttcctg cacggcaacc ccaccagcag
     6001 ctacgtgtgg cgcaacatca tcccccacgt ggcccccacc caccgcacta tcgcccccga
     6061 cctgatcggc atgggcaaga gcgacaagcc cgacctgggc tacttcttcg acgaccacgt
     6121 gcgcttcatg gacgccttca tcgaggccct gggcctggag gaggtggtgc tggtgatcca
     6181 cgactggggc agcgccctgg gcttccactg ggccaagcgc aaccccgagc gcgtgaaggg
     6241 catcgccttc atggagttca tccgccccat ccccacctgg gacgagtggc ccgagttcgc
     6301 ccgcgagacc ttccaggcct tccgcaccac cgacgtgggc cgcaagctga tcatcgacca
     6361 gaacgtgttc atcgagggca ccctgcccat gggcgtggtg cgccccctga ccgaggtgga
     6421 gatggaccac taccgcgagc ccttcctgaa ccccgtggac cgcgagcccc tgtggcgctt
     6481 ccccaacgag ctgcccatcg ccggcgagcc cgccaacatc gtggccctgg tggaggagta
     6541 catggactgg ctgcaccaga gccccgtgcc caagctgctg ttctggggca cccccggcgt
     6601 gctgatcccc cccgccgagg ccgcccgcct ggccaagagc ctgcccaacg ttaaggccgt
     6661 ggacatcggc cccggcctga acctgctcca ggaggacaac cccgacctga tcggcagcga
     6721 gatcgcccgc tggctgagca ccctcgagat cagcggcaaa gatgagttgt agaagcttat
     6781 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     6841 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     6901 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     6961 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     7021 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     7081 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     7141 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     7201 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     7261 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     7321 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     7381 ccgtcgaccc gggcggccgc ttcgagcaga catgataaga tacattgatg agtttggaca
     7441 aaccacaact agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc
     7501 tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt
     7561 tatgtttcag gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa
     7621 atgtggtaaa atcgataa
//
