LOCUS       sequence-487229-        7190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  ER targeted expression of green glucose sensor with non-responsive 
            HaloTag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7190)
  AUTHORS   Marvin JS, Machler P, Meng C, Ates T, Patel RH, Adhikari R, Makurath
            MA, Ku Z, Feliciano D, Atasoy D, Cui G, Kleinfeld D, Brown TA
  TITLE     iGlucoSnFR2: A genetically encoded fluorescent sensor for measuring 
            intracellular or extracellular glucose in vivo in mouse brain.
  JOURNAL   Sci Adv. 2025 Nov 14;11(46):eadz3889. doi: 10.1126/sciadv.adz3889. 
            Epub 2025 Nov 12.
  PUBMED    41223288
REFERENCE   2  (bases 1 to 7190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-11-14"; volume: "11(46):eadz3889. doi"; pages: " 
            10.1126/sciadv.adz3889. Epub 2025 Nov 12"
COMMENT     Sequence Label: pAAV.hSynap.(ER).iGlucoSnFR2.HaloTag
FEATURES             Location/Qualifiers
     source          1..7190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(237..825)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(317..336)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(996..1787,1788..1856))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1619..1638
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1857..1961)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1987..2442)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2124..2145)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2336..2355
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   2514..2643
                     /label=AAV2 ITR
     promoter        2719..3166
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     primer_bind     4445..4466
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4569..4590)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     misc_feature    6141..6729
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6194..6214)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6612..6623)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(6732..6748)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6733..6749)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    6778..6899
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6789..6808)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6843..6862
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   7061..7190
                     /label=AAV2 ITR
ORIGIN
        1 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
       61 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
      121 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      181 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      241 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      301 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      361 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      421 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      481 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      541 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      601 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      661 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      721 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      781 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      841 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      901 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
      961 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     1021 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     1081 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     1141 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     1201 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     1261 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     1321 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     1381 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     1441 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     1501 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     1561 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     1621 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     1681 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     1741 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     1801 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     1861 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     1921 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     1981 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     2041 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     2101 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     2161 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     2221 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     2281 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     2341 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     2401 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcccattcg ccattcaggc
     2461 tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctgcgcg
     2521 ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc tttggtcgcc
     2581 cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc actaggggtt
     2641 ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc tctaggaaga
     2701 tccagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc ggggtggggg
     2761 tgcctacctg acgaccggcc ccgacccact ggacaagcac ccaaccccca ttccccaaat
     2821 tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg cgtgcgcact
     2881 gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc gccaccgccg
     2941 cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc cccttcccgg
     3001 ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg cgaggcgcga
     3061 gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag cgctgcctca
     3121 gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcca attcaagctg
     3181 ctagcaagga tcccgccacc atgctgctat ccgtgccgct gctgctcggc ctcctcggcc
     3241 tggccatcgc cagatctaag ctggagattt tctcttggtg ggctggggat gaaggccctg
     3301 ccctggaggc actcatcaga ctgtataaac agaagtatcc tggagtagag gtgataaacg
     3361 ccactgtcac cggaggtgcc ggtgtaaatg ctcgcgctgt ccttaagaca aggatgcttg
     3421 gtggtgatcc tcctgataca tttcaagttg cagcagggat ggagttgata ggcacatggg
     3481 tggtggctaa cagaatggag gatctcagcg ccttgtttcg acaagagggt tggcttcaag
     3541 ccttccccaa gggccttatt gatctgatta gttataaagg tggtatatgg agtgtgcccg
     3601 ttaatattca caggtcaaac gtaatgtggt atttgcctgc aaagctaaaa gagtggggag
     3661 ttaacccccc acggacttgg gatgagtttc ttgccacctg tcaaacactt aaacaaaagg
     3721 gtctggaagc tccactggct ctcggggaaa attggacaca acaacacctc tgggagagtg
     3781 tcgccttggc tgtattgggg cctgatgatt ggaataatct gtggaatggg aagctgaagt
     3841 tcacagaccc taaagcagtt agggcatggg aagtctttgg ccgcgttttg gactgtgcca
     3901 acaaggatgc agcaggcttg tcttggcagc aagctgtcga tcgggtagta caggggaaag
     3961 ccgcttttaa cgtaatgggc gactgggcag caggatatat gactaccact ctcaagttga
     4021 agcctggtac agatttcgca tgggcaccaa gtcccgggac acagggcgtc tttatgatgg
     4081 tttccgactc tttcggactt cccaaaggag ccaaaaatcg acaaaacgct atcaactggc
     4141 ttcgattggt cggctctaaa gaaggtcaag atacattcaa cccactgaaa gggtccatcg
     4201 ctgctcggct cgatagcgac ccaagcaagt atccgtgtaa cgtctatatc accgccgaca
     4261 agcagaagaa cggcatcaag gcgaacttca agatccgcca caacgtggag gacggcagcg
     4321 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     4381 ccgacaacca ctacctggtg acccagtccg tgctgagcaa agaccccaac gagaagcgcg
     4441 atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc
     4501 tgtacaaggg cggtaccgga gggagcatga gcaagggcga ggagctgttc accggggtgg
     4561 tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgcgcggcg
     4621 agggcgaggg cgatgccacc aacggcaagc tgaccctgaa gttcatctgc accaccggca
     4681 agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca
     4741 gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct
     4801 acgtccagga gcgcaccatc agcttcaagg acgacggcac ctacaagacc cgcgccgagg
     4861 tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg
     4921 aggacggcaa catcctgggg cacaagctgg agtacaactt taacaacccc aacgcatacg
     4981 gacagagtgc aatgcgagat tggcggagta atcgcatcgt tggctccctg gtagcaggag
     5041 cagtggcccc tgaaagctcc atgtcacaat tcggaacagt aatggaaatt ttcctgcaaa
     5101 cacgcaaccc acaagcagca gcaaatgcag cacaagctat tgctgaccaa gttggcctgg
     5161 gtcgccttgg tcagctgcag ggcggaggct cgggcggagg tgggtcgggt ggcggcggat
     5221 caatggccga gatcggcacc ggcttcccct tcgaccccca ctacgtggag gtgctgggcg
     5281 agcgcatgca ctacgttgac gtgggccccc gcgacggcac ccccgtgctg ttcctgcacg
     5341 gcaaccccac cagcagctac gtgtggcgca acatcatccc ccacgtggcc cccacccacc
     5401 gcactatcgc ccccgacctg atcggcatgg gcaagagcga caagcccgac ctgggctact
     5461 tcttcgacga ccacgtgcgc ttcatggacg ccttcatcga ggccctgggc ctggaggagg
     5521 tggtgctggt gatccacgac tggggcagcg ccctgggctt ccactgggcc aagcgcaacc
     5581 ccgagcgcgt gaagggcatc gccttcatgg agttcatccg ccccatcccc acctgggacg
     5641 agtggcccga gttcgcccgc gagaccttcc aggccttccg caccaccgac gtgggccgca
     5701 agctgatcat cgaccagaac gtgttcatcg agggcaccct gcccatgggc gtggtgcgcc
     5761 ccctgaccga ggtggagatg gaccactacc gcgagccctt cctgaacccc gtggaccgcg
     5821 agcccctgtg gcgcttcccc aacgagctgc ccatcgccgg cgagcccgcc aacatcgtgg
     5881 ccctggtgga ggagtacatg gactggctgc accagagccc cgtgcccaag ctgctgttct
     5941 ggggcacccc cggcgtgctg atcccccccg ccgaggccgc ccgcctggcc aagagcctgc
     6001 ccaacgttaa ggccgtggac atcggccccg gcctgaacct gctccaggag gacaaccccg
     6061 acctgatcgg cagcgagatc gcccgctggc tgagcaccct cgagatcagc ggcaaagatg
     6121 agttgtagaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa agattgactg
     6181 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     6241 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc
     6301 tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt
     6361 ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga
     6421 ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct
     6481 gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat
     6541 cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct
     6601 gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc
     6661 tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg
     6721 cctccccgca tcgataccgt cgacctcgac ccgggcggcc gcttcgagca gacatgataa
     6781 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     6841 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     6901 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     6961 aaagcaagta aaacctctac aaatgtggta aaatcgataa ggatcttcct agagcatggc
     7021 tacgtagata agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag
     7081 ttggccactc cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc
     7141 cgacgcccgg gctttgcccg ggcggcctca gtgagtgagc gagcgcgcag
//
