LOCUS       sequence-487459-        9341 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses unspliced EPB41L4A-AS1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9341)
  AUTHORS   Monziani A,  Unfried JP, Cvetanovic T, Ulitsky I
  TITLE     EPB41L4A-AS1 long noncoding RNA acts in both cis- and trans-acting 
            transcriptional regulation and controls nucleolar biology
  JOURNAL   bioRxiv 2025.04.08.647803
REFERENCE   2  (bases 1 to 9341)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.04.08.647803"
COMMENT     Sequence Label: pLV-Puro-CMV-EPB41L4A-AS1-unspliced-MUT- delta...
FEATURES             Location/Qualifiers
     source          1..9341
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     enhancer        2019..2322
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2323..2526
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2477..2497
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    2547..2571
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(4014..4038)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    4056..4644
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4109..4129)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4527..4538)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        4675..5174
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(4710..4733)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5107..5126
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5191..5790
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5191..5210)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5687..5707
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             5862..6095
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6167..6288
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6204..6223)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6258..6277
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6328..6463
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6483..6502)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6484..6502)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6512..6529)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6512..6528)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6521..6543)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6670..7125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6757..6776)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6967..6988
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7151..7255
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7256..7324,7325..8116)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7474..7493)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8287..8875
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8776..8795
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9029..9046
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    9163..9184
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(9199..9204,9205..9222,9223..9229)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9237..9253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     9242..9264
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     9261..9277
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9261..9277
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     9296..9316
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        9298..9316
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttgta gttattaata gtaatcaatt
     1981 acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact tacggtaaat
     2041 ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat gacgtatgtt
     2101 cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta tttacggtaa
     2161 actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc tattgacgtc
     2221 aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg ggactttcct
     2281 acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg gttttggcag
     2341 tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct ccaccccatt
     2401 gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa atgtcgtaac
     2461 aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt ctatataagc
     2521 agagctggtt tagtgaaccg tcagatccaa gtttgtacaa aaaagcaggc ttcctttctt
     2581 ccttttggtg cgagcttgct gtggtttttg ctctgggtcc tctgggatgg cgcctggctg
     2641 tggccgcgtg gtctctcacg caggggcgcc gggcggggga acgcggccac cctgagtctg
     2701 gtgagtcgac tgcggcggcc tgtgtccgaa gtgtccgggg ccgtgaacaa gggcagcggc
     2761 ctggcctcag gcctgcgttc ccacgtttgg aaacggggag cttcgtcgat ttgtgtttac
     2821 atcatcgact atgccaggga gttctccaga taagcctggt tttattttcg tcagtgaaaa
     2881 ggccttaccg tataactgac tttatgcttg ccctgccccc gtataaaata ccttagaagt
     2941 agagtgcctg gttacagctg tttccacgtg cggtgctcgt cgggagtgat cacctaccct
     3001 acaggtgagt tttcacgttc gtgcaagacc agtcgccatt taaaacgcat cgcatttcac
     3061 ttttcattat taagtcggat tttaaatcgt gagaaaattt ctctgaaatg tattgtccgt
     3121 ttttaggctg taatcggcat tactgtcagc cagtcagcaa ccttatgcca taaagcctac
     3181 ctcacgcagt gtcagccttt gtgttgccca ttcactttgg aaactagtga atgtggtgtc
     3241 aaaaaaggcg taacgctttg cagccttttc ctgcccttaa atttgatacc tttggtgtag
     3301 gagctgcata agtaacagtt gctgctttta cgtttccacg cgtgatcttg accctgctag
     3361 ccttaagtgt atggtttctc ttagccagtt ctaatttttg ttcaggtgga agatggatgc
     3421 ctgaagtgta gactgctgct agctgaatac catctgggag cataaaggtg acctgaaggt
     3481 agggtgatat gtcttaaagc actttgtaat gggaattttt atcacctttt aaattggggt
     3541 tccttctcta gtgagtttta atgtcagtgg tacattcgta gtgttgctct gtctgtagct
     3601 attaaggtga gttaataaat gggatagcct ccacagctta tttttgggaa ggttttgctg
     3661 atacttcctg agaagcccag ggaaataaat acgcatagta ctggcattct gcatctcttt
     3721 aagatttgtt tttatgtgta gtaattgagt tttttaaaag cttgtgaaat cgcaggcata
     3781 ttaccaagtt cttgattaaa atgtaataca aaaatatttg ctgtcgaatt gagtacttta
     3841 ttttttctct taggatgtcc ttggtgagga ttttgaaaat ttgatcttca caagagttgc
     3901 ctggatcatt tgaaatttct gggagtctga ggagtactga cataattacc tgctggagtc
     3961 tgtaaataca catttaagac agtgaggatg tgaataaata tattaatgca cttacccagc
     4021 tttcttgtac aaagtggtga taatcgaatt ccgataatca acctctggat tacaaaattt
     4081 gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg
     4141 ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt
     4201 ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg
     4261 tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc
     4321 agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg
     4381 cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt
     4441 tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc tggattctgc
     4501 gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg
     4561 gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga
     4621 tctccctttg ggccgcctcc ccgcatcggg aattcccgcg gttcgaattc taccgggtag
     4681 gggaggcgct tttcccaagg cagtctggag catgcgcttt agcagccccg ctgggcactt
     4741 ggcgctacac aagtggcctc tggcctcgca cacattccac atccaccggt aggcgccaac
     4801 cggctccgtt ctttggtggc cccttcgcgc caccttctac tcctccccta gtcaggaagt
     4861 tcccccccgc cccgcagctc gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca
     4921 ctagtctcgt gcagatggac agcaccgctg agcaatggaa gcgggtaggc ctttggggca
     4981 gcggccaata gcagctttgc tccttcgctt tctgggctca gaggctggga aggggtgggt
     5041 ccgggggcgg gctcaggggc gggctcaggg gcggggcggg cgcccgaagg tcctccggag
     5101 gcccggcatt ctgcacgctt caaaagcgca cgtctgccgc gctgttctcc tcttcctcat
     5161 ctccgggcct ttcgacctca cgtggccacc atgaccgagt acaagcccac ggtgcgcctc
     5221 gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac
     5281 cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa
     5341 gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc
     5401 gccgcggtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag
     5461 atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa
     5521 ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc
     5581 tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg
     5641 gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc
     5701 tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc
     5761 tggtgcatga cccgcaagcc cggtgcctga ggtaccttta agaccaatga cttacaaggc
     5821 agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc
     5881 ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag accagatctg
     5941 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6001 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6061 cagacccttt tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat
     6121 tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc
     6181 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6241 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct
     6301 ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     6361 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc
     6421 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac gtacccaatt
     6481 cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa cgtcgtgact
     6541 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
     6601 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
     6661 gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     6721 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     6781 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     6841 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     6901 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     6961 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     7021 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     7081 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg
     7141 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     7201 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     7261 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     7321 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     7381 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     7441 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     7501 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     7561 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     7621 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     7681 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     7741 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     7801 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     7861 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     7921 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     7981 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     8041 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     8101 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     8161 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     8221 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     8281 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     8341 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     8401 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     8461 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     8521 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     8581 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     8641 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     8701 cgaagagaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     8761 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     8821 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     8881 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatattctt
     8941 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9001 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     9061 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     9121 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     9181 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     9241 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
     9301 taaccctcac taaagggaac aaaagctgga gctgcaagct t
//
