LOCUS       sequence-487485-        8978 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Secretory granule marker for endocrine cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8978)
  TITLE     Martin lab plasmids
REFERENCE   2  (bases 1 to 8978)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: NPY EGFP
FEATURES             Location/Qualifiers
     source          1..8978
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        1..304
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        305..508
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     459..479
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     505..529
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      859..868
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(865..867,868..870,871..1584)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(865..867,868..870,871..1581)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(910..931)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1171..1190)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1518..1539
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        1613..2112
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1648..1671)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     2045..2064
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             2133..2732
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2133..2152)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2629..2649
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2746..3334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2799..2819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3217..3228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3541..4174
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(4303..4319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4303..4319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4316..4338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4327..4343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4351..4357,4358..4375,4376..4381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4396..4417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4534..4551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4705..5293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4785..4804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5464..6255,6256..6324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6087..6106
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6325..6429)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    6477..6611
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6514..6533)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6568..6587
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             6779..7412
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    7459..7584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8081..8314
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8499..8543
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    8806..8921
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
ORIGIN
        1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
       61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
      121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
      241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
      361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
      421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
      481 acggtgggag gtctatataa gcagagctcg tttagtgaac cgtcagatcg cctggagacg
      541 ccatccacgc tgttttgacc tccatagaag acaccgactc tactagagga tcgctagcgc
      601 taccggactc agatctcgag ctcaagcttg ggctgcaggt ccgcgccagc caccatgcta
      661 ggtaacaagc gactggggct gtccggactg accctcgccc tgtccctgct cgtgtgcctg
      721 ggtgcgctgg ccgaggcgta cccctccaag ccggacaacc cgggcgagga cgcaccagcg
      781 gaggacatgg ccagatacta ctcggcgctg cgacactaca tcaacctcat caccaggcag
      841 agatatggaa aaccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg
      901 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      961 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
     1021 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
     1081 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
     1141 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
     1201 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
     1261 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
     1321 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
     1381 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
     1441 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
     1501 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
     1561 ggcatggacg agctgtacaa gtaaagcggc cgcgactcta gataattcta ccgggtaggg
     1621 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     1681 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     1741 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     1801 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     1861 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     1921 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     1981 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     2041 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     2101 ccgggccttt cgacctgcag cccaagctta ccatgaccga gtacaagccc acggtgcgcc
     2161 tcgccacccg cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact
     2221 accccgccac gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc
     2281 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
     2341 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
     2401 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
     2461 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
     2521 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
     2581 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
     2641 tctacgagcg gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca
     2701 cctggtgcat gacccgcaag cccggtgcct gaccgcgtct ggaacaatca acctctggat
     2761 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     2821 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     2881 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     2941 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     3001 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     3061 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     3121 tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc tgctcgcctg tgttgccacc
     3181 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     3241 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     3301 acgagtcgga tctccctttg ggccgcctcc ccgcctggaa ttaattctgc agtcgagacc
     3361 tagaaaaaca tggagcaatc acaagtagca atacagcagc taccaatgct gattgtgcct
     3421 ggctagaagc acaagaggag gaggaggtgg gttttccagt cacacctcag gtacctttaa
     3481 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     3541 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     3601 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     3661 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     3721 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     3781 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     3841 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     3901 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     3961 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     4021 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4081 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4141 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     4201 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     4261 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     4321 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     4381 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     4441 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     4501 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     4561 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     4621 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4681 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4741 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4801 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4861 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4921 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4981 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     5041 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     5101 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     5161 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     5221 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     5281 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     5341 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     5401 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     5461 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     5521 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     5581 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     5641 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     5701 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     5761 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     5821 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     5881 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     5941 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     6001 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     6061 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     6121 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     6181 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     6241 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     6301 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     6361 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     6421 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatcaact
     6481 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     6541 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     6601 atgtctggat caactggata actcaagcta accaaaatca tcccaaactt cccaccccat
     6661 accctattac cactgccaat tacctgtggt ttcatttact ctaaacctgt gattcctctg
     6721 aattattttc attttaaaga aattgtattt gttaaatatg tactacaaac ttagtagttg
     6781 gaagggctaa ttcactccca aagaagacaa gatatccttg atctgtggat ctaccacaca
     6841 caaggctact tccctgatta gcagaactac acaccagggc caggggtcag atatccactg
     6901 acctttggat ggtgctacaa gctagtacca gttgagccag ataaggtaga agaggccaat
     6961 aaaggagaga acaccagctt gttacaccct gtgagcctgc atgggatgga tgacccggag
     7021 agagaagtgt tagagtggag gtttgacagc cgcctagcat ttcatcacgt ggcccgagag
     7081 ctgcatccgg agtacttcaa gaactgctga tatcgagctt gctacaaggg actttccgct
     7141 ggggactttc cagggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     7201 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     7261 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7321 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7381 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7441 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7501 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7561 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7621 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7681 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7741 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7801 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7861 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7921 gcggccggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     7981 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8041 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8101 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8161 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8221 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8281 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8341 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8401 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8461 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8521 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     8581 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     8641 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     8701 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     8761 gagacagatc cattcgatta gtgaacggat ctcgacggta tcgcctttaa aagaaaaggg
     8821 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     8881 actaaagaat tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac
     8941 agcagagatc cagtttatcg ataagcttgg gagttccg
//
