LOCUS       sequence-487559-       10421 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of MLS-TIR1-Linker-mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10421)
  TITLE     Hengbin lab plasmids 2025
REFERENCE   2  (bases 1 to 10421)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: MLS-TIR1-Linker-mCherry
FEATURES             Location/Qualifiers
     source          1..10421
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             8..188
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    235..360
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    853..1086
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1271..1315
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1613..1730
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        1781..2029
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     1781..1801
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     1952..1971
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2051..2126
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        2209..2420
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             2596..4320
                     /codon_start=1
                     /product="rice transport inhibitor response 1 protein"
                     /label=OsTIR1
                     /note="F-box protein that mediates auxin-dependent 
                     interaction of a ubiquitin ligase with an auxin-inducible 
                     degron (Tan et al., 2007)"
                     /translation="MTYFPEEVVEHIFSFLPAQRDRNTVSLVCKVWYEIERLSRRGVFV
                     GNCYAVRAGRVAARFPNVRALTVKGKPHFADFNLVPPDWGGYAGPWIEAAARGCHGLEE
                     LRMKRMVVSDESLELLARSFPRFRALVLISCEGFSTDGLAAVASHCKLLRELDLQENEV
                     EDRGPRWLSCFPDSCTSLVSLNFACIKGEVNAGSLERLVSRSPNLRSLRLNRSVSVDTL
                     AKILLRTPNLEDLGTGNLTDDFQTESYFKLTSALEKCKMLRSLSGFWDASPVCLSFIYP
                     LCAQLTGLNLSYAPTLDASDLTKMISRCVKLQRLWVLDCISDKGLQVVASSCKDLQELR
                     VFPSDFYVAGYSAVTEEGLVAVSLGCPKLNSLLYFCHQMTNAALVTVAKNCPNFTRFRL
                     CILEPGKPDVVTSQPLDEGFGAIVRECKGLQRLSISGLLTDKVFMYIGKYAKQLEMLSI
                     AFAGDSDKGMMHVMNGCKNLRKLEIRDSPFGDAALLGNFARYETMRSLWMSSCNVTLKG
                     CQVLASKMPMLNVEVINERDGSNEMEENHGDLPKVEKLYVYRTTAGARDDAPNFVKIL"
     CDS             4321..4344
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4402..5112
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4547..4565)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4815..4834
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     LTR             5346..5579
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5605..5622)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    5611..5835
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      5881..6309
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5968..5987)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6178..6199
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6318..6338)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6323..6652
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6503..6638
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6565..6584
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        6700..6747
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             6766..7140
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7270..7391
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7307..7326)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7361..7380
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7440..7456)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7440..7456)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7453..7475)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7464..7480
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7488..7494,7495..7512,7513..7518))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7533..7554
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7671..7688)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7842..8430)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7922..7941)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8601..9392,9393..9461))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9224..9243
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9462..9566)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9641..9660)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9832..10211
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10213..10411
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10366..10386
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
       61 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
      121 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
      181 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
      241 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
      301 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
      361 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
      421 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
      481 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
      541 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
      601 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
      661 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
      721 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
      781 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
      841 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
      901 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
      961 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     1021 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     1081 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     1141 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     1201 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     1261 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     1321 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     1381 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     1441 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     1501 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     1561 atcggcactg cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa
     1621 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
     1681 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
     1741 acagggacag cagagatcca gtttggttaa ttaaggtacc gagggcctat ttcccatgat
     1801 tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa ttaatttgac
     1861 tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat ttcttgggta
     1921 gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg taacttgaaa
     1981 gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg gcccaacatc
     2041 ctcgtgtcca gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac
     2101 ttgaaaaagt ggcaccgagt cggtgctctc cctgagcttc agggaggttt tttgaattcg
     2161 ctagctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca
     2221 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga
     2281 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg
     2341 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg
     2401 ggtttgccgc cagaacacag gaccggttct agagccgcca ccatgttggg gtttgtgggt
     2461 cgggtggccg ctgctccggc ctccggggcc ttgcggagac tcaccccttc agcgtcgctg
     2521 cccccagctc agctcttact gcgggccgct ccgacggcgg tccatcctgt cagggactat
     2581 gcggcgcaaa gcgctatgac atactttcct gaagaggtcg tcgaacacat ttttagcttc
     2641 ctgcctgcac agagagatag aaacacagtg agcctggtct gcaaagtgtg gtacgagatc
     2701 gaacgcctga gccggagagg agtgttcgtc ggcaactgct atgctgtgag agcaggcagg
     2761 gtcgccgcta ggtttccaaa tgtgcgcgca ctgaccgtca aggggaaacc ccacttcgcc
     2821 gactttaacc tggtgccccc tgattgggga ggatacgccg gcccttggat cgaggcagcc
     2881 gctcgcggct gtcatggact ggaggaactg cgcatgaagc gaatggtggt ctctgacgaa
     2941 agtctggagc tgctggctcg gagcttccct aggtttcgcg cactggtgct gatttcttgc
     3001 gaaggcttca gcaccgatgg actggcagcc gtggcctccc actgtaagct gctgcgggag
     3061 ctggacctcc aggagaatga agtggaggat agaggcccca gatggctgtc ttgcttccca
     3121 gactcatgta ccagcctggt gtccctgaac tttgcctgca tcaaaggcga agtgaatgct
     3181 gggtccctgg agcggctggt ctcaagaagc cccaacctga ggtctctgcg gctgaaccgg
     3241 agcgtgagcg tggacactct ggctaagatt ctgctgagaa cccctaacct ggaggatctg
     3301 ggaaccggca atctgacaga cgatttccag acagaatcct actttaaact gacttctgcc
     3361 ctggagaagt gtaaaatgct gaggagtctg tcaggattct gggatgcttc acccgtgtgc
     3421 ctgagcttta tctaccctct gtgtgcacag ctgacaggcc tgaacctgag ctatgcacca
     3481 accctggacg ccagtgatct gacaaagatg atctcacgct gcgtgaaact ccagcgactg
     3541 tgggtgctgg actgtatttc cgataagggg ctccaggtgg tcgccagctc ctgcaaggac
     3601 ctccaggagc tgagagtgtt cccatctgat ttttacgtgg ccggatatag tgctgtcact
     3661 gaggaaggcc tggtggcagt ctcactggga tgcccaaagc tgaacagcct gctgtatttc
     3721 tgtcatcaga tgactaatgc tgcactggtg accgtcgcca agaactgccc taatttcacc
     3781 cgatttcggc tgtgtattct ggaaccaggc aaacccgacg tggtcacatc ccagccactg
     3841 gatgaagggt ttggagctat cgtgagagag tgcaagggac tccagaggct gagcatttcc
     3901 ggcctgctga cagacaaagt gttcatgtac atcggcaagt atgctaagca gctggagatg
     3961 ctgagcattg catttgccgg agactccgat aagggcatga tgcacgtgat gaacgggtgt
     4021 aagaatctgc gaaaactgga aatccgggac agccctttcg gggatgccgc tctgctggga
     4081 aactttgcca gatacgagac aatgaggagc ctgtggatgt ctagttgcaa tgtgactctg
     4141 aagggctgtc aggtcctggc tagtaaaatg cctatgctga acgtggaagt cattaatgag
     4201 cgggacgggt ctaacgaaat ggaggaaaat catggcgacc tgccaaaggt ggagaaactg
     4261 tatgtgtatc ggaccaccgc aggggcaaga gatgatgctc ccaactttgt gaagattctg
     4321 gattacaaag acgatgacga taagggatcc ggtggaggcg gttcaggcgg aggtggctct
     4381 ggcggtggcg gatcgctcga gatggtgagc aagggcgagg aggataacat ggccatcatc
     4441 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
     4501 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
     4561 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
     4621 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
     4681 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
     4741 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
     4801 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
     4861 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
     4921 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
     4981 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
     5041 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
     5101 ctgtacaagt agggcgcgcc ggggtctttg tcctctgtac tgtctctctc cttgccccta
     5161 acccaaaaag cttcattttt ctgtgtaggc tgcacaagag ccttgattga agatatattc
     5221 tttctgaaca gtatttaagg tttccaataa aatgtacacc cctcagaacg tacggtcgac
     5281 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     5341 gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg
     5401 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     5461 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     5521 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     5581 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     5641 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     5701 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     5761 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     5821 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc
     5881 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
     5941 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
     6001 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
     6061 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
     6121 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
     6181 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
     6241 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
     6301 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     6361 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     6421 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     6481 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     6541 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
     6601 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
     6661 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
     6721 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
     6781 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
     6841 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     6901 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     6961 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     7021 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     7081 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     7141 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     7201 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     7261 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     7321 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     7381 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     7441 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     7501 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     7561 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     7621 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     7681 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     7741 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     7801 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     7861 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     7921 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     7981 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8041 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8101 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8161 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8221 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8281 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8341 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8401 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8461 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     8521 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     8581 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     8641 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     8701 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     8761 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     8821 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     8881 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     8941 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     9001 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     9061 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     9121 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     9181 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     9241 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     9301 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     9361 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     9421 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     9481 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     9541 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     9601 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     9661 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     9721 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     9781 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     9841 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     9901 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     9961 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
    10021 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
    10081 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
    10141 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
    10201 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
    10261 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
    10321 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
    10381 ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg c
//
