LOCUS       sequence-487566-        3686 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Ae aegypti UbL40 promoter expressing mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3686)
  AUTHORS   Nevard K, Ang JXD, Anderson MAE, Gonzalez E, Shackleford L, Alphey L
  TITLE     Harnessing the highly adaptable barnase-barstar system for genetic 
            biocontrol of Aedes aegypti.
  JOURNAL   Commun Biol. 2025 Aug 4;8(1):1154. doi: 10.1038/s42003-025-08588-6.
  PUBMED    40760041
REFERENCE   2  (bases 1 to 3686)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Commun 
            Biol."; date: "2025-08-4"; volume: "8(1):1154. doi"; pages: " 
            10.1038/s42003-025-08588-6"
COMMENT     Sequence Label: AGG1983 UbL40-mCherry
FEATURES             Location/Qualifiers
     source          1..3686
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..28
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     12..28
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             496..561
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     polyA_signal    1424..1545
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1461..1480)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1515..1534
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1602..1618)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1602..1618)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1615..1637)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     rep_origin      complement(1790..2378)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1870..1889)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    complement(2495..2519)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(join(2619..3410,3411..3479))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3242..3261
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3480..3584)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     join(3683..3686,1..19)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 gtcacgacgt tgtaaaacga cggccagtca tgcataatcc gcacgcatct ggaataagga
       61 agtgccattc cgcctgacct cttgcatgcg tgcacagaat atgattgcat ttcatgaccg
      121 ggtatccgtt cggcgcaggc cgctgaactg aaattcagtt ttgacagctc cactgacttc
      181 aagctctttt tcaccgaaac acatctcgga agagacacgt tgggaaagag taagtttgat
      241 ttttgccgtt tttcgtggat ttttagtgat aattcgtggt ttttttatgc tagcaaccta
      301 aattattgat agctaaagtt agaaattgtg aaatattgtt gaaattccca cattattcgt
      361 agtttattcg atttgttgta gttgatgcat tctaacctca atgtggtttc gtctccgtcc
      421 aattccgatg ttattgtcca gttcgttaat ttttggtgtt ttctccccga cttttccaca
      481 gactgatcca agatggacta taaggaccac gacggagact acaaggatca tgatattgat
      541 tacaaagacg atgacgataa gggagatccc accccaccca agaagaagcg caaagctagc
      601 gtgtcgaagg gagaagaaga taacatggcc atcatcaagg agttcatgcg tttcaaagtc
      661 cacatggaag gatcggtgaa cggacacgaa tttgaaatcg aaggagaagg agaaggacgc
      721 ccgtacgaag gaacccagac cgccaagctg aaagtgacga agggaggacc gctgccgttc
      781 gcctgggata tcctgtcgcc gcagttcatg tacggatcga aggcctacgt gaagcacccg
      841 gccgatatcc cggattacct gaagctgtcg ttcccggaag gattcaagtg ggaacgtgtg
      901 atgaactttg aagatggagg agtggtgact gtgacccagg attcgtcgtt gcaggatgga
      961 gagttcatct acaaggtgaa gctgcgtgga accaacttcc cgtcggatgg accagtgatg
     1021 cagaagaaga ctatgggctg ggaagcctcg tcggaacgca tgtacccgga agatggagcc
     1081 ctgaagggag aaatcaagca gcgtctgaag ctgaaggatg gaggacacta cgatgccgaa
     1141 gtgaagacca cctacaaggc caagaagccg gtgcagctgc cgggagccta caacgtgaac
     1201 atcaagctgg atatcacctc gcacaacgaa gattacacca tcgtggaaca gtacgaacgt
     1261 gccgaaggac gccactcgac cggaggaatg gatgaactgt acaagtaact agctgatgat
     1321 cataatcagc cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct
     1381 ccccctgaac ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc
     1441 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
     1501 actgcattct agttgtggtt tgtccaaact catcaatgta tcttaaggct aggtggaggc
     1561 tcagtgatga taagtctgcg atggtggatg catgtgtcat ggtcatagct gtttcctgtg
     1621 tgaaattgtt atccgctcag agggcacaat cctattccgc gctatccgac aatctccaag
     1681 acattaggtg gagttcagtt cggcgtatgg catatgtcgc tggaaagaac atgtgagcaa
     1741 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     1801 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     1861 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     1921 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     1981 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     2041 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     2101 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     2161 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     2221 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     2281 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     2341 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     2401 cggggtctga cgctctattc aacaaagccg ccgtcccgtc aagtcagcgt aaatgggtag
     2461 ggggcttcaa atcgtcctcg tgataccaat tcggagcctg cttttttgta caaacttgtt
     2521 gataatggca attcaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     2581 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     2641 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     2701 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     2761 gagccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     2821 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     2881 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     2941 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     3001 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     3061 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     3121 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     3181 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     3241 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     3301 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     3361 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     3421 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     3481 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     3541 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     3601 gtgccagata cctgaaacaa aacccatcgt acggccaagg aagtctccaa taactgtgat
     3661 ccaccacaag cgccagggtt ttccca
//
