LOCUS       sequence-487660-        1905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of WAChR-1f opsin fusion protein: 
            {WAChR-1f}-{Kir2.1 Golgi tag}-{mScarlet3}-{Kv2.1 soma tag}-{Kir2.1 
            ER tag}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
  TITLE     WAChRs are excitatory opsins sensitive to indoor lighting
  JOURNAL   bioRxiv 2025.09.12.675947; doi: 
            https://doi.org/10.1101/2025.09.12.675947
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947"
COMMENT     Sequence Label: pAAV-EF1a>WAChR_1f-G-mScarlet3-Soma-E; WPRE3-B...
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1133..1151)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atgtctgcct gccctacacc ccctactgtg gtggccccca ctgaccagtc tcccaaccct
       61 aatggaggca ccacctcctg tgcccaggct gcccctagcc cccagatgag catgaactac
      121 accctgctgg ccaccattga tggcttctct gctgtgatcc tgttgggctc aggggtggtg
      181 ttctggtact acagctggag gggcacaggc tttggagccc accctcaccc caagagaagg
      241 cccctggagc tgaacaccct gagtgctgcc attgcctttt tgacgggatt gatgtatgtg
      301 gccaagacct ctgactttgc cagagacagc acctctgagt tctcattaaa tcgatactgg
      361 tacagtgact acattgtgac ctgccccttc attgtgatgg atctgtgttt tacggtggat
      421 gtgccctata agctggcctt cacaggcagc acagctgtcc tgctggtgat tgccctggcc
      481 agctttattg tgagaagccc tgagaagtac atctactttg ccattggctg caccctgttc
      541 agcatcttct tctacttctt tttcaatgag gtgagaaaaa gaatcaggta tgtgcctgac
      601 tgtgccaaga aggacctgga gagagccatg ttcattttct ttggcttctg gcccatgttt
      661 ccggtattat ggctgctggg cttccacacc actggagtga tctctgagga ggcctttgcc
      721 atcatccatg ccttcctgga cattacctgc aagaccatct ttggggtgtt catgctgaga
      781 tgtagactgc acattgagga ctatgcctgg gatgagctga agaggcagag acaggagatt
      841 gatgaggaga accagaagac cctgacagct gctggggctg atggagccac agtgatgatg
      901 cctgtggagg aggatgaccc ctatgcctct ggggctgctg ccaagagcag gatcacctct
      961 gaaggggagt acatccccct ggaccagatt gacatcaatg tgatggacag cactgaggct
     1021 gtgatcaagg agttcatgag gttcaaggtg cacatggagg gcagcatgaa tggccatgag
     1081 tttgagattg agggagaagg agagggcaga ccctatgagg gcacccagac agccaagctg
     1141 aaggtgacca agggaggccc cctgcccttc agctgggaca tcctgagccc ccagttcatg
     1201 tatggcagca gggccttcat caagcaccca gctgacatcc ctgactactg gaagcaaagc
     1261 ttccctgagg gcttcaagtg ggagagagtg atgatctttg aggatggggg cacagtgagt
     1321 gtgacccagg acaccagcct ggaggatggc accctgatct acaaggtgaa gctgagagga
     1381 ggcaacttcc ctcctgatgg cccagtgatg cagaagagga ccatgggctg ggaggccagc
     1441 acagagaggc tgtaccctga ggatgtggtg ctgaaggggg acatcaagat ggccctgaga
     1501 ctgaaggatg gaggcaggta cctggctgat ttcaagacca cctacaaggc caagaagcct
     1561 gtccagatgc ctggagcctt caacattgac agaaagctgg acatcaccag ccacaatgag
     1621 gactacacag tggtggagca gtatgagaga tctgtggcca ggcactccac aggaggctct
     1681 gggggcagcc agagccaacc catcctgaac accaaggaga tggcccctca gagcaagccc
     1741 cctgaggagc tggagatgag cagcatgccc agccctgtgg ccccactgcc tgccaggaca
     1801 gagggagtga ttgacatgag aagcatgagc tccattgaca gcttcatcag ctgtgccaca
     1861 gacttcccag aggccaccag gttcttctgc tatgaaaatg aggtt
//
