LOCUS       sequence-487662-        1911 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of WAChR-1m opsin fusion protein: 
            {WAChR-1m}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER
            tag}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1911)
  AUTHORS   Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
  TITLE     WAChRs are excitatory opsins sensitive to indoor lighting
  JOURNAL   bioRxiv 2025.09.12.675947; doi: 
            https://doi.org/10.1101/2025.09.12.675947
REFERENCE   2  (bases 1 to 1911)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947"
COMMENT     Sequence Label: pAAV-EF1a>WAChR_1m-G-mScarlet-Soma-E; WPRE3-BG...
FEATURES             Location/Qualifiers
     source          1..1911
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1003..1695
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWDRVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1133..1151)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1401..1420
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgagcgcct gtcctacacc ccctaccgtg gtggccccca cagatcaaag ccccaatcct
       61 aacggaggca ccacctcttg tgctcaggct gccccttctc ctcagatgag catgaattac
      121 accctgcttg caaccatcga cggatttagc gccgtgattc tgcttggaag cggcgtggtg
      181 ttctggtact actcttggag gggaaccggc tttggagccc acccccatcc caagagaaga
      241 cccctggaac tgaataccct gtctgccgcc atcgccttct taaccggcct gatgtacgtg
      301 gccaagacca gcgactttgc tagggacagc accagcgagt ttagcctgaa caggtactgg
      361 tacctggact acatcgtgac atgccccctg atcgtgatgg acctgtgctt caccgtggac
      421 gtgccctaca agctggcctt caccggcagc accgccgtgc tgctggtgat cgccctcgcc
      481 agcttcatcg tgaggagccc cgagaagtac atctacttcg ctatcggctg caccctgttt
      541 agcattttct tctacttctt tttcaacgag gtgagaaaga ggatcaggta cgtgcccgac
      601 tgcgccaaga aggacctgga gagggccatg ttcatcttct tcggcttctg gcccatgttc
      661 cccgtgctgt ggctgctggg ctttcacacc accggcgtga tcagcgagga ggcctttgct
      721 atcattcacg ccttcctaga cattacctgc aagaccattt tcggcgtgtt catgctgagg
      781 tgcagactgc acatcgagga ctacgcctgg gatgagctga aaaggcagag acaggagatc
      841 gacgaggaga atcagaaaac cttgaccgcc gctggcgccg atggcgccac cgtgatgatg
      901 cccgttgaag aagacgaccc ctacgctagc ggagcggccg ccaagagcag gatcaccagc
      961 gagggcgagt acatccccct ggaccagatc gatatcaacg tggtgagcaa gggcgaggca
     1021 gtgatcaagg agttcatgcg gttcaaggtg cacatggagg gctccatgaa cggccacgag
     1081 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     1141 aaggtgacca agggtggccc cctgcccttc tcctgggaca tcctgtcccc tcagttcatg
     1201 tacggctcca gggccttcac caagcacccc gccgacatcc ccgactacta taagcagtcc
     1261 ttccccgagg gcttcaagtg ggaccgcgtg atgaacttcg aggacggcgg cgccgtgacc
     1321 gtgacccagg acacctccct ggaggacggc accctgatct acaaggtgaa gctccgcggc
     1381 accaacttcc ctcctgacgg ccccgtaatg cagaagaaga caatgggctg ggaagcgtcc
     1441 accgagcggt tgtaccccga ggacggcgtg ctgaagggcg acattaagat ggccctgcgc
     1501 ctgaaggacg gcgggcgcta cctggcggac ttcaagacca cctacaaggc caagaagccc
     1561 gtgcagatgc ccggcgccta caacgtggac cgcaagttgg acatcacctc ccacaacgag
     1621 gactacaccg tggtggaaca gtacgaacgc tccgagggcc gccactccac cggcggcatg
     1681 gacgagctgt acaagcagtc ccagcccatc ctcaacacca aggagatggc cccgcagagc
     1741 aagcctccag aggagctgga gatgagcagc atgcccagcc ccgtggcccc tctgcccgca
     1801 cgcacggagg gcgtcatcga catgcggagc atgtccagca ttgacagctt catcagctgt
     1861 gccacggact tccctgaagc caccagattc ttctgctacg agaacgaggt g
//
