LOCUS       sequence-487664-        1905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive expression of WAChR-1s opsin fusion protein: 
            {WAChR-1s}-{Kir2.1 Golgi tag}-{mScarlet}-{Kv2.1 soma tag}-{Kir2.1 ER
            tag}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
  TITLE     WAChRs are excitatory opsins sensitive to indoor lighting
  JOURNAL   bioRxiv 2025.09.12.675947; doi: 
            https://doi.org/10.1101/2025.09.12.675947
REFERENCE   2  (bases 1 to 1905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947"
COMMENT     Sequence Label: pAAV-EF1a>WAChR_1s-G-mScarlet-Soma-E; WPRE3-BG...
FEATURES             Location/Qualifiers
     source          1..1905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1133..1151)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 atgtctgcct gccccacacc ccctacagtg gtggccccca cagaccagag cccaaaccct
       61 aatggaggca ccaccagctg tgcccaggct gcccccagcc ctcagatgag catgaactac
      121 accctgttgg ccaccattga tggcttctct gctgtgattc tgctgggcag tggggtggtg
      181 ttctggtact acagctggag gggcacaggc tttggagccc atccccaccc taagaggagg
      241 cccctggagc tgaacaccct gtctgctgcc attgctttct taacgggatt gatgtatgtg
      301 gccaagacct ctgactttgc cagagacagc acctcagagt tcagcctgaa cagatactgg
      361 tactctgact acattgtgac ctgccccctg atagtgatgg acctgtgctt cacagtggat
      421 gtgccctaca agctggcctt cacaggcagc acagctgtcc tgctggtgat tgccctggcc
      481 agcttcattg tgagaagccc tgagaagtac atctattttg ccattggctg caccctgttc
      541 agcatcttct tctacttctt ctttaatgag gtgagaaaga gaatcaggta tgtgcctgac
      601 tgtgccaaga aggacctgga gagggccatg tggatcttct ttggcttctg gcccatgttc
      661 ccagtcctgt ggctgctggg cttccacacc actggagtga tctctgagga ggcctttgcc
      721 atcatccatg ccttcttaga cattacctgc aagaccatct ttggggtgtt catgctgaga
      781 tgcagactgc acattgagga ctatgcctgg gatgagctga agaggcagag acaggagatt
      841 gatgaggaga accagaagac cctgacagct gctggggctg atggagccac tgtgatgatg
      901 cctgtggagg aggatgaccc ctatgcctct ggagctgctg ccaagagcag gatcacctct
      961 gagggagagt acatccccct ggaccagatt gacatcaatg tgatggacag cacagaggct
     1021 gtgatcaagg agttcatgag gttcaaggtg cacatggagg gcagcatgaa tggccatgag
     1081 tttgagattg agggagaagg agagggcagg ccctatgagg gcacccagac agccaagctg
     1141 aaggtgacca agggaggccc cctgcccttc agctgggaca tcctgagccc acagttcatg
     1201 tatggctcca gagccttcat caagcaccct gctgacatcc ctgactactg gaagcagagc
     1261 ttccctgagg gcttcaagtg ggagagggtg atgatctttg aggatggggg cactgtgtct
     1321 gtgacccagg acaccagcct ggaggatggc accctgatct acaaggtgaa gctgagggga
     1381 ggcaacttcc ctcctgatgg cccagtgatg cagaagagga ccatgggctg ggaggcctcc
     1441 acagagaggc tgtaccctga ggatgtggtg ctgaaggggg acatcaagat ggccctgagg
     1501 ctgaaggatg gaggcagata cctggctgac ttcaagacca cctacaaggc caagaaacct
     1561 gtgcagatgc ctggggcctt caacattgac agaaagctgg acatcactag ccacaatgag
     1621 gactacacag ttgtggagca gtatgagaga tctgtggcca ggcacagcac tggaggctct
     1681 gggggctccc agagccagcc catcctgaac accaaggaga tggcccctca gagcaagccc
     1741 cctgaggagc tggagatgag cagcatgccc agccctgtgg ctcccctgcc tgccagaaca
     1801 gagggggtga ttgacatgag aagcatgagc tccattgaca gcttcatctc ctgtgccaca
     1861 gacttcccag aggccaccag attcttctgc tatgaaaatg aggtt
//
