LOCUS       sequence-487666-        1884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of WAChR-1f opsin fusion 
            protein:{WAChR-1f}-{Kir2.1 Golgi tag}-{mScarlet3}-{Kv2.1 soma 
            tag}-{Kir2.1 ER tag}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1884)
  AUTHORS   Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
  TITLE     WAChRs are excitatory opsins sensitive to indoor lighting
  JOURNAL   bioRxiv 2025.09.12.675947; doi: 
            https://doi.org/10.1101/2025.09.12.675947
REFERENCE   2  (bases 1 to 1884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947"
COMMENT     Sequence Label: pAAV-EF1a>FLEX-WAChR_1f-G-mScarlet3-Soma-E;
            WP...
FEATURES             Location/Qualifiers
     source          1..1884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     734..752
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gaacctggtg gcctctggga agtctgtggc acagctgatg aagctgtcaa tggagctcat
       61 gcttctcatg tcaatcactc cctctgtcct ggcaggcagt ggggccacag ggctgggcat
      121 gctgctcatc tccagctcct cagggggctt gctctgaggg gccatctcct tggtgttcag
      181 gatgggttgg ctctggctgc ccccagagcc tcctgtggag tgcctggcca cagatctctc
      241 atactgctcc accactgtgt agtcctcatt gtggctggtg atgtccagct ttctgtcaat
      301 gttgaaggct ccaggcatct ggacaggctt cttggccttg taggtggtct tgaaatcagc
      361 caggtacctg cctccatcct tcagtctcag ggccatcttg atgtccccct tcagcaccac
      421 atcctcaggg tacagcctct ctgtgctggc ctcccagccc atggtcctct tctgcatcac
      481 tgggccatca ggagggaagt tgcctcctct cagcttcacc ttgtagatca gggtgccatc
      541 ctccaggctg gtgtcctggg tcacactcac tgtgccccca tcctcaaaga tcatcactct
      601 ctcccacttg aagccctcag ggaagctttg cttccagtag tcagggatgt cagctgggtg
      661 cttgatgaag gccctgctgc catacatgaa ctgggggctc aggatgtccc agctgaaggg
      721 cagggggcct cccttggtca ccttcagctt ggctgtctgg gtgccctcat agggtctgcc
      781 ctctccttct ccctcaatct caaactcatg gccattcatg ctgccctcca tgtgcacctt
      841 gaacctcatg aactccttga tcacagcctc agtgctgtcc atcacattga tgtcaatctg
      901 gtccaggggg atgtactccc cttcagaggt gatcctgctc ttggcagcag ccccagaggc
      961 ataggggtca tcctcctcca caggcatcat cactgtggct ccatcagccc cagcagctgt
     1021 cagggtcttc tggttctcct catcaatctc ctgtctctgc ctcttcagct catcccaggc
     1081 atagtcctca atgtgcagtc tacatctcag catgaacacc ccaaagatgg tcttgcaggt
     1141 aatgtccagg aaggcatgga tgatggcaaa ggcctcctca gagatcactc cagtggtgtg
     1201 gaagcccagc agccataata ccggaaacat gggccagaag ccaaagaaaa tgaacatggc
     1261 tctctccagg tccttcttgg cacagtcagg cacatacctg attctttttc tcacctcatt
     1321 gaaaaagaag tagaagaaga tgctgaacag ggtgcagcca atggcaaagt agatgtactt
     1381 ctcagggctt ctcacaataa agctggccag ggcaatcacc agcaggacag ctgtgctgcc
     1441 tgtgaaggcc agcttatagg gcacatccac cgtaaaacac agatccatca caatgaaggg
     1501 gcaggtcaca atgtagtcac tgtaccagta tcgatttaat gagaactcag aggtgctgtc
     1561 tctggcaaag tcagaggtct tggccacata catcaatccc gtcaaaaagg caatggcagc
     1621 actcagggtg ttcagctcca ggggccttct cttggggtga gggtgggctc caaagcctgt
     1681 gcccctccag ctgtagtacc agaacaccac ccctgagccc aacaggatca cagcagagaa
     1741 gccatcaatg gtggccagca gggtgtagtt catgctcatc tgggggctag gggcagcctg
     1801 ggcacaggag gtggtgcctc cattagggtt gggagactgg tcagtggggg ccaccacagt
     1861 agggggtgta gggcaggcag acat
//
