LOCUS       sequence-487670-        2004 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutive bicistronic expression via P2A cleavage: 
            {WAChR-1m}-{Kir2.1 Golgi tag}-{Kv2.1 soma tag}-{Kir2.1 ER tag}-{HA 
            tag} + {mScarlet}.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Tose AJ, Nava AA, McGrath SN, Mardinly AR, Naka A
  TITLE     WAChRs are excitatory opsins sensitive to indoor lighting
  JOURNAL   bioRxiv 2025.09.12.675947; doi: 
            https://doi.org/10.1101/2025.09.12.675947
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.09.12.675947; doi: https://doi.org/10.1101/2025.09.12.675947"
COMMENT     Sequence Label: pAAV-EF1a>WAChR_1m-G-Soma-E-HA-P2A-mScarlet;
            W...
FEATURES             Location/Qualifiers
     source          1..2004
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1219..1245
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1255..1311
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1312..2004
                     /codon_start=1
                     /product="bright monomeric red fluorescent protein evolved 
                     from a synthetic template (Bindels et al., 2016)"
                     /label=mScarlet
                     /translation="VSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFSWDILSPQFMYGSRAFTKHPADIPDYYKQSFPEGFKWDRVMNFEDGGA
                     VTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIKM
                     ALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRHS
                     TGGMDELYK"
     primer_bind     complement(1442..1460)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     1710..1729
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgagcgcct gtcctacacc ccctaccgtg gtggccccca cagatcaaag ccccaatcct
       61 aacggaggca ccacctcttg tgctcaggct gccccttctc ctcagatgag catgaattac
      121 accctgcttg caaccatcga cggatttagc gccgtgattc tgcttggaag cggcgtggtg
      181 ttctggtact actcttggag gggaaccggc tttggagccc acccccatcc caagagaaga
      241 cccctggaac tgaataccct gtctgccgcc atcgccttct taaccggcct gatgtacgtg
      301 gccaagacca gcgactttgc tagggacagc accagcgagt ttagcctgaa caggtactgg
      361 tacctggact acatcgtgac atgccccctg atcgtgatgg acctgtgctt caccgtggac
      421 gtgccctaca agctggcctt caccggcagc accgccgtgc tgctggtgat cgccctcgcc
      481 agcttcatcg tgaggagccc cgagaagtac atctacttcg ctatcggctg caccctgttt
      541 agcattttct tctacttctt tttcaacgag gtgagaaaga ggatcaggta cgtgcccgac
      601 tgcgccaaga aggacctgga gagggccatg ttcatcttct tcggcttctg gcccatgttc
      661 cccgtgctgt ggctgctggg ctttcacacc accggcgtga tcagcgagga ggcctttgct
      721 atcattcacg ccttcctaga cattacctgc aagaccattt tcggcgtgtt catgctgagg
      781 tgcagactgc acatcgagga ctacgcctgg gatgagctga aaaggcagag acaggagatc
      841 gacgaggaga atcagaaaac cttgaccgcc gctggcgccg atggcgccac cgtgatgatg
      901 cccgttgaag aagacgaccc ctacgctagc ggagcggccg ccaagagcag gatcaccagc
      961 gagggcgagt acatccccct ggaccagatc gacatcaacg tgcagtccca gcccatcctc
     1021 aacaccaagg agatggcccc gcagagcaag cctccagagg agctggagat gagcagcatg
     1081 cccagccccg tggcccctct gcccgcacgc acggagggcg tcatcgacat gcggagcatg
     1141 tccagcattg acagcttcat cagctgtgcc acggacttcc ctgaagccac cagattcttc
     1201 tgctacgaga acgaggtgta cccatacgat gttccagatt acgctggcag cggcgccacc
     1261 aacttctccc tgctgaagca ggccggcgac gtggaggaga accccggccc cgtgagcaag
     1321 ggcgaggcag tgatcaagga gttcatgcgg ttcaaggtgc acatggaggg ctccatgaac
     1381 ggccacgagt tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc
     1441 gccaagctga aggtgaccaa gggtggcccc ctgcccttct cctgggacat cctgtcccct
     1501 cagttcatgt acggctccag ggccttcacc aagcaccccg ccgacatccc cgactactat
     1561 aagcagtcct tccccgaggg cttcaagtgg gaccgcgtga tgaacttcga ggacggcggc
     1621 gccgtgaccg tgacccagga cacctccctg gaggacggca ccctgatcta caaggtgaag
     1681 ctccgcggca ccaacttccc tcctgacggc cccgtaatgc agaagaagac aatgggctgg
     1741 gaagcgtcca ccgagcggtt gtaccccgag gacggcgtgc tgaagggcga cattaagatg
     1801 gccctgcgcc tgaaggacgg cgggcgctac ctggcggact tcaagaccac ctacaaggcc
     1861 aagaagcccg tgcagatgcc cggcgcctac aacgtggacc gcaagttgga catcacctcc
     1921 cacaacgagg actacaccgt ggtggaacag tacgaacgct ccgagggccg ccactccacc
     1981 ggcggcatgg acgagctgta caag
//
