LOCUS       sequence-487719-        3144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Temperature-Inducible promoter, not including 
            ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3144)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_cI857_PR
FEATURES             Location/Qualifiers
     source          1..3144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(849..867)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(850..867)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(883..899)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(883..899)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1014..1602)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1094..1113)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1717..1819
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1820..2479
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1886..1905)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2592..3047
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2679..2698)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2889..2910
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3066..3083
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3067..3083
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3090..3109
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3090..3108
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gtcagccaaa cgtttcttca ggccactgac tagcgataac tttccccaca
       61 acggaacaac tctcattgca tgggatcatt gggtactgtg ggtttagtgg ttgtaaaaac
      121 acctgaccgc tatccctgat cagtttcttg aaggtaaact catcaccccc aagtctggct
      181 atgcagaaat cacctggctc aacagcctgc tcagggtcaa cgagaattaa cattccgtca
      241 ggaaagcttg gcttggagcc tgttggtgcg gtcatggaat taccttcaac ctcaagccag
      301 aatgcagaat cactggcttt tttggttgtg cttacccatc tctccgcatc acctttggta
      361 aaggttctaa gctcaggtga gaacatccct gcctgaacat gagaaaaaac agggtactca
      421 tactcacttc taagtgacgg ctgcatacta accgcttcat acatctcgta gatttctctg
      481 gcgattgaag ggctaaattc ttcaacgcta actttgagaa tttttgtaag caatgcggcg
      541 ttataagcat ttaatgcatt gatgccatta aataaagcac caacgcctga ctgccccatc
      601 cccatcttgt ctgcgacaga ttcctgggat aagccaagtt catttttctt tttttcataa
      661 attgctttaa ggcgacgtgc gtcctcaagc tgctcttgtg ttaatggttt cttttttgtg
      721 ctcatacgtt aaatctatca ccgcaaggga taaatatcta acaccgtgcg tgttgactat
      781 tttacctctg gcggtgataa tggttgcaca aacgagacca ctcatcgccc aatcactagt
      841 gaattcgttc tatagtgtca cctaaatacc atagagccgt tggtcatagc tgtttcctgt
      901 ccaggcatca tgacacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      961 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
     1021 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
     1081 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
     1141 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
     1201 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
     1261 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
     1321 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
     1381 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
     1441 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
     1501 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
     1561 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
     1621 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
     1681 ttatcaaaaa ggatcttcac ctagatcctt ttgtgatgat cggcacgtaa gaggttccaa
     1741 ctttcaccat aatgaaataa gatcactacc gggcgtattt tttgagttat cgagattttc
     1801 aggagctaag gaagctaaaa tggagaaaaa aatcactgga tataccaccg ttgatatatc
     1861 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
     1921 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
     1981 gttttatccg gcctttattc acattcttgc ccgcctgatg aatgctcatc cggaattccg
     2041 tatggcaatg aaagacggtg agctggtgat atgggatagt gttcaccctt gttacaccgt
     2101 tttccatgag caaactgaaa cgttttcatc gctctggagt gaataccacg acgatttccg
     2161 gcagtttcta cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
     2221 ccctaaaggg tttattgaga atatgttttt cgtgtcagcc aatccctggg tgagtttcac
     2281 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcaccatggg
     2341 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgattcagg ttcatcatgc
     2401 cgtttgtgat ggcttccatg tcggcagaat gcttaatgaa ttacaacagt actgcgatga
     2461 gtggcagggc ggggcgtaat ttttttaagg cagttattgg tgcccttaaa cgcctggttg
     2521 ctacgcctga ataagtgata ataagcggat gaatggcaga aattcgatga taagctgtca
     2581 aacatgatgt tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2641 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2701 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2761 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     2821 acgtagtggg ccagcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2881 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     2941 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     3001 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaattttaa cattgtagaa
     3061 acgcatgtaa aacgacggcc agtgaattgt aatacgactc actatagggt gaattccgct
     3121 taggacctta gatattgatc tcaa
//
