LOCUS       sequence-487727-        2393 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 T7 RNA polymerase and IPTG/Lactose-dependent 
            promoter, not including ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_PARTPRM_PT7lac
FEATURES             Location/Qualifiers
     source          1..2393
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..30
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    31..55
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(98..116)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(99..116)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(132..148)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(132..148)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(263..851)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(343..362)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        966..1068
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1069..1728
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1135..1154)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1841..2296
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1928..1947)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2138..2159
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2315..2332
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2316..2332
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        2339..2357
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gtaatacgac tcactatagg ggaattgtga gcggataaca attcccccaa
       61 acgagaccac tcatcgccca atcactagtg aattcgttct atagtgtcac ctaaatacca
      121 tagagccgtt ggtcatagct gtttcctgtc caggcatcat gacacggtta tccacagaat
      181 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
      241 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
      301 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
      361 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
      421 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
      481 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
      541 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
      601 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
      661 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
      721 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
      781 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
      841 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
      901 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
      961 tgtgatgatc ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg
     1021 ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa
     1081 atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca
     1141 tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt
     1201 ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc
     1261 cgcctgatga atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata
     1321 tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg
     1381 ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg
     1441 gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc
     1501 gtgtcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac
     1561 aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg
     1621 atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg
     1681 cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc
     1741 agttattggt gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg
     1801 aatggcagaa attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca
     1861 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     1921 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     1981 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2041 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt
     2101 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2161 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2221 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2281 ttaacgctta caattttaac attgtagaaa cgcatgtaaa acgacggcca gtgaattgta
     2341 atacgactca ctatagggtg aattccgctt aggaccttag atattgatct caa
//
