LOCUS       sequence-487824-        2365 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS5
FEATURES             Location/Qualifiers
     source          1..2365
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(70..88)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(71..88)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(104..120)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(104..120)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        938..1040
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1041..1700
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1107..1126)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1813..2268
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1900..1919)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2110..2131
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2287..2304
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2288..2304
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2311..2330
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2311..2329
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agactggtga ggaggtgaga atgcgagacc actcatcgcc caatcactag
       61 tgaattcgtt ctatagtgtc acctaaatac catagagccg ttggtcatag ctgtttcctg
      121 tccaggcatc atgacacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
      181 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttgtgatga tcggcacgta agaggttcca
      961 actttcacca taatgaaata agatcactac cgggcgtatt ttttgagtta tcgagatttt
     1021 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat
     1081 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata
     1141 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca
     1201 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc
     1261 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg
     1321 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc
     1381 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt
     1441 tccctaaagg gtttattgag aatatgtttt tcgtgtcagc caatccctgg gtgagtttca
     1501 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg
     1561 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg
     1621 ccgtttgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg
     1681 agtggcaggg cggggcgtaa tttttttaag gcagttattg gtgcccttaa acgcctggtt
     1741 gctacgcctg aataagtgat aataagcgga tgaatggcag aaattcgatg ataagctgtc
     1801 aaacatgatg ttacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     1861 gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     1921 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     1981 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt
     2041 cacgtagtgg gccagcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     2101 tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt
     2161 cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     2221 aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttta acattgtaga
     2281 aacgcatgta aaacgacggc cagtgaattg taatacgact cactataggg tgaattccgc
     2341 ttaggacctt agatattgat ctcaa
//
