LOCUS       sequence-487839-        2366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2366)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS20
FEATURES             Location/Qualifiers
     source          1..2366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(71..89)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(72..89)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(105..121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(105..121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(236..824)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(316..335)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        939..1041
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1042..1701
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1108..1127)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1814..2269
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1901..1920)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2111..2132
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2288..2305
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2289..2305
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2312..2331
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2312..2330
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agaaacctca aggaattccc aatgcgagac cactcatcgc ccaatcacta
       61 gtgaattcgt tctatagtgt cacctaaata ccatagagcc gttggtcata gctgtttcct
      121 gtccaggcat catgacacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      181 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      241 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      301 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      361 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      421 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      481 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc
      541 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca
      601 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact
      661 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg
      721 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt
      781 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct
      841 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga
      901 gattatcaaa aaggatcttc acctagatcc ttttgtgatg atcggcacgt aagaggttcc
      961 aactttcacc ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt
     1021 tcaggagcta aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata
     1081 tcccaatggc atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat
     1141 aaccagaccg ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac
     1201 aagttttatc cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattc
     1261 cgtatggcaa tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc
     1321 gttttccatg agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc
     1381 cggcagtttc tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat
     1441 ttccctaaag ggtttattga gaatatgttt ttcgtgtcag ccaatccctg ggtgagtttc
     1501 accagttttg atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg
     1561 ggcaaatatt atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat
     1621 gccgtttgtg atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat
     1681 gagtggcagg gcggggcgta atttttttaa ggcagttatt ggtgccctta aacgcctggt
     1741 tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgat gataagctgt
     1801 caaacatgat gttacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     1861 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     1921 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     1981 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     2041 tcacgtagtg ggccagcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     2101 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     2161 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     2221 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttt aacattgtag
     2281 aaacgcatgt aaaacgacgg ccagtgaatt gtaatacgac tcactatagg gtgaattccg
     2341 cttaggacct tagatattga tctcaa
//
