LOCUS       sequence-487840-        2369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS21
FEATURES             Location/Qualifiers
     source          1..2369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(74..92)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(75..92)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(108..124)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(108..124)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(239..827)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(319..338)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        942..1044
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1045..1704
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1111..1130)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1817..2272
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1904..1923)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2114..2135
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2291..2308
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2292..2308
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2315..2334
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2315..2333
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agaggcgaca ggcggatcta tccaatgcga gaccactcat cgcccaatca
       61 ctagtgaatt cgttctatag tgtcacctaa ataccataga gccgttggtc atagctgttt
      121 cctgtccagg catcatgaca cggttatcca cagaatcagg ggataacgca ggaaagaaca
      181 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
      241 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
      301 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
      361 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
      421 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
      481 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
      541 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
      601 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
      661 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct
      721 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
      781 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
      841 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
      901 tgagattatc aaaaaggatc ttcacctaga tccttttgtg atgatcggca cgtaagaggt
      961 tccaactttc accataatga aataagatca ctaccgggcg tattttttga gttatcgaga
     1021 ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat
     1081 atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc
     1141 tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag
     1201 cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa
     1261 ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac
     1321 accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat
     1381 ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc
     1441 tatttcccta aagggtttat tgagaatatg tttttcgtgt cagccaatcc ctgggtgagt
     1501 ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc
     1561 atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat
     1621 catgccgttt gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc
     1681 gatgagtggc agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct
     1741 ggttgctacg cctgaataag tgataataag cggatgaatg gcagaaattc gatgataagc
     1801 tgtcaaacat gatgttacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     1861 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     1921 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     1981 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     2041 ggttcacgta gtgggccagc gccctgatag acggtttttc gccctttgac gttggagtcc
     2101 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     2161 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     2221 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat tttaacattg
     2281 tagaaacgca tgtaaaacga cggccagtga attgtaatac gactcactat agggtgaatt
     2341 ccgcttagga ccttagatat tgatctcaa
//
