LOCUS       sequence-487842-        2361 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2361)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2361)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS23
FEATURES             Location/Qualifiers
     source          1..2361
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(66..84)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(67..84)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(100..116)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(100..116)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(231..819)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(311..330)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        934..1036
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1037..1696
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1103..1122)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1809..2264
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1896..1915)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2106..2127
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2283..2300
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2284..2300
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2307..2326
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2307..2325
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agaatagagt cgacgaatgc gagaccactc atcgcccaat cactagtgaa
       61 ttcgttctat agtgtcacct aaataccata gagccgttgg tcatagctgt ttcctgtcca
      121 ggcatcatga cacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
      181 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
      241 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
      301 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
      361 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
      421 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
      481 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
      541 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
      601 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
      661 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
      721 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
      781 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
      841 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
      901 tcaaaaagga tcttcaccta gatccttttg tgatgatcgg cacgtaagag gttccaactt
      961 tcaccataat gaaataagat cactaccggg cgtatttttt gagttatcga gattttcagg
     1021 agctaaggaa gctaaaatgg agaaaaaaat cactggatat accaccgttg atatatccca
     1081 atggcatcgt aaagaacatt ttgaggcatt tcagtcagtt gctcaatgta cctataacca
     1141 gaccgttcag ctggatatta cggccttttt aaagaccgta aagaaaaata agcacaagtt
     1201 ttatccggcc tttattcaca ttcttgcccg cctgatgaat gctcatccgg aattccgtat
     1261 ggcaatgaaa gacggtgagc tggtgatatg ggatagtgtt cacccttgtt acaccgtttt
     1321 ccatgagcaa actgaaacgt tttcatcgct ctggagtgaa taccacgacg atttccggca
     1381 gtttctacac atatattcgc aagatgtggc gtgttacggt gaaaacctgg cctatttccc
     1441 taaagggttt attgagaata tgtttttcgt gtcagccaat ccctgggtga gtttcaccag
     1501 ttttgattta aacgtggcca atatggacaa cttcttcgcc cccgttttca ccatgggcaa
     1561 atattatacg caaggcgaca aggtgctgat gccgctggcg attcaggttc atcatgccgt
     1621 ttgtgatggc ttccatgtcg gcagaatgct taatgaatta caacagtact gcgatgagtg
     1681 gcagggcggg gcgtaatttt tttaaggcag ttattggtgc ccttaaacgc ctggttgcta
     1741 cgcctgaata agtgataata agcggatgaa tggcagaaat tcgatgataa gctgtcaaac
     1801 atgatgttac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     1861 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     1921 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     1981 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     2041 tagtgggcca gcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     2101 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     2161 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     2221 aaaatttaac gcgaatttta acaaaatatt aacgcttaca attttaacat tgtagaaacg
     2281 catgtaaaac gacggccagt gaattgtaat acgactcact atagggtgaa ttccgcttag
     2341 gaccttagat attgatctca a
//
