LOCUS       sequence-487843-        2362 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2362)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2362)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS24
FEATURES             Location/Qualifiers
     source          1..2362
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(67..85)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(68..85)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(101..117)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(101..117)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(232..820)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(312..331)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        935..1037
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1038..1697
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1104..1123)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1810..2265
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1897..1916)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2107..2128
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2284..2301
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2285..2301
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2308..2327
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2308..2326
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agagagtaat atttcaaatg cgagaccact catcgcccaa tcactagtga
       61 attcgttcta tagtgtcacc taaataccat agagccgttg gtcatagctg tttcctgtcc
      121 aggcatcatg acacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
      181 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
      241 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
      301 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
      361 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
      421 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
      481 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
      541 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
      601 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
      661 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
      721 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
      781 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
      841 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
      901 atcaaaaagg atcttcacct agatcctttt gtgatgatcg gcacgtaaga ggttccaact
      961 ttcaccataa tgaaataaga tcactaccgg gcgtattttt tgagttatcg agattttcag
     1021 gagctaagga agctaaaatg gagaaaaaaa tcactggata taccaccgtt gatatatccc
     1081 aatggcatcg taaagaacat tttgaggcat ttcagtcagt tgctcaatgt acctataacc
     1141 agaccgttca gctggatatt acggcctttt taaagaccgt aaagaaaaat aagcacaagt
     1201 tttatccggc ctttattcac attcttgccc gcctgatgaa tgctcatccg gaattccgta
     1261 tggcaatgaa agacggtgag ctggtgatat gggatagtgt tcacccttgt tacaccgttt
     1321 tccatgagca aactgaaacg ttttcatcgc tctggagtga ataccacgac gatttccggc
     1381 agtttctaca catatattcg caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc
     1441 ctaaagggtt tattgagaat atgtttttcg tgtcagccaa tccctgggtg agtttcacca
     1501 gttttgattt aaacgtggcc aatatggaca acttcttcgc ccccgttttc accatgggca
     1561 aatattatac gcaaggcgac aaggtgctga tgccgctggc gattcaggtt catcatgccg
     1621 tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt acaacagtac tgcgatgagt
     1681 ggcagggcgg ggcgtaattt ttttaaggca gttattggtg cccttaaacg cctggttgct
     1741 acgcctgaat aagtgataat aagcggatga atggcagaaa ttcgatgata agctgtcaaa
     1801 catgatgtta cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     1861 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     1921 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     1981 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     2041 gtagtgggcc agcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     2101 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     2161 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     2221 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aattttaaca ttgtagaaac
     2281 gcatgtaaaa cgacggccag tgaattgtaa tacgactcac tatagggtga attccgctta
     2341 ggaccttaga tattgatctc aa
//
