LOCUS       sequence-487846-        2382 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS27
FEATURES             Location/Qualifiers
     source          1..2382
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(87..105)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(88..105)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(121..137)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(121..137)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(252..840)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(332..351)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        955..1057
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1058..1717
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1124..1143)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1830..2285
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1917..1936)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2127..2148
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2304..2321
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2305..2321
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2328..2347
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2328..2346
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agatttctcc cataataact cgaaccatta ccgagcaatg cgagaccact
       61 catcgcccaa tcactagtga attcgttcta tagtgtcacc taaataccat agagccgttg
      121 gtcatagctg tttcctgtcc aggcatcatg acacggttat ccacagaatc aggggataac
      181 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
      241 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
      301 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
      361 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
      421 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
      481 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
      541 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
      601 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
      661 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
      721 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
      781 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
      841 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
      901 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt gtgatgatcg
      961 gcacgtaaga ggttccaact ttcaccataa tgaaataaga tcactaccgg gcgtattttt
     1021 tgagttatcg agattttcag gagctaagga agctaaaatg gagaaaaaaa tcactggata
     1081 taccaccgtt gatatatccc aatggcatcg taaagaacat tttgaggcat ttcagtcagt
     1141 tgctcaatgt acctataacc agaccgttca gctggatatt acggcctttt taaagaccgt
     1201 aaagaaaaat aagcacaagt tttatccggc ctttattcac attcttgccc gcctgatgaa
     1261 tgctcatccg gaattccgta tggcaatgaa agacggtgag ctggtgatat gggatagtgt
     1321 tcacccttgt tacaccgttt tccatgagca aactgaaacg ttttcatcgc tctggagtga
     1381 ataccacgac gatttccggc agtttctaca catatattcg caagatgtgg cgtgttacgg
     1441 tgaaaacctg gcctatttcc ctaaagggtt tattgagaat atgtttttcg tgtcagccaa
     1501 tccctgggtg agtttcacca gttttgattt aaacgtggcc aatatggaca acttcttcgc
     1561 ccccgttttc accatgggca aatattatac gcaaggcgac aaggtgctga tgccgctggc
     1621 gattcaggtt catcatgccg tttgtgatgg cttccatgtc ggcagaatgc ttaatgaatt
     1681 acaacagtac tgcgatgagt ggcagggcgg ggcgtaattt ttttaaggca gttattggtg
     1741 cccttaaacg cctggttgct acgcctgaat aagtgataat aagcggatga atggcagaaa
     1801 ttcgatgata agctgtcaaa catgatgtta cgcgccctgt agcggcgcat taagcgcggc
     1861 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
     1921 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
     1981 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
     2041 tgattagggt gatggttcac gtagtgggcc agcgccctga tagacggttt ttcgcccttt
     2101 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
     2161 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
     2221 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
     2281 aattttaaca ttgtagaaac gcatgtaaaa cgacggccag tgaattgtaa tacgactcac
     2341 tatagggtga attccgctta ggaccttaga tattgatctc aa
//
