LOCUS       sequence-487847-        2383 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Ribosome binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2383)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2383)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_RBS_RBS28
FEATURES             Location/Qualifiers
     source          1..2383
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(88..106)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(89..106)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(122..138)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(122..138)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(253..841)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(333..352)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        956..1058
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1059..1718
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1125..1144)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1831..2286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1918..1937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2128..2149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2305..2322
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2306..2322
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2329..2348
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2329..2347
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcgcaa agagggaacg atttaggcca attattaaaa ccgacccaat gcgagaccac
       61 tcatcgccca atcactagtg aattcgttct atagtgtcac ctaaatacca tagagccgtt
      121 ggtcatagct gtttcctgtc caggcatcat gacacggtta tccacagaat caggggataa
      181 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
      241 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
      301 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
      361 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
      421 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
      481 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
      541 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
      601 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
      661 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
      721 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
      781 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
      841 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
      901 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt tgtgatgatc
      961 ggcacgtaag aggttccaac tttcaccata atgaaataag atcactaccg ggcgtatttt
     1021 ttgagttatc gagattttca ggagctaagg aagctaaaat ggagaaaaaa atcactggat
     1081 ataccaccgt tgatatatcc caatggcatc gtaaagaaca ttttgaggca tttcagtcag
     1141 ttgctcaatg tacctataac cagaccgttc agctggatat tacggccttt ttaaagaccg
     1201 taaagaaaaa taagcacaag ttttatccgg cctttattca cattcttgcc cgcctgatga
     1261 atgctcatcc ggaattccgt atggcaatga aagacggtga gctggtgata tgggatagtg
     1321 ttcacccttg ttacaccgtt ttccatgagc aaactgaaac gttttcatcg ctctggagtg
     1381 aataccacga cgatttccgg cagtttctac acatatattc gcaagatgtg gcgtgttacg
     1441 gtgaaaacct ggcctatttc cctaaagggt ttattgagaa tatgtttttc gtgtcagcca
     1501 atccctgggt gagtttcacc agttttgatt taaacgtggc caatatggac aacttcttcg
     1561 cccccgtttt caccatgggc aaatattata cgcaaggcga caaggtgctg atgccgctgg
     1621 cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt cggcagaatg cttaatgaat
     1681 tacaacagta ctgcgatgag tggcagggcg gggcgtaatt tttttaaggc agttattggt
     1741 gcccttaaac gcctggttgc tacgcctgaa taagtgataa taagcggatg aatggcagaa
     1801 attcgatgat aagctgtcaa acatgatgtt acgcgccctg tagcggcgca ttaagcgcgg
     1861 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     1921 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     1981 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     2041 ttgattaggg tgatggttca cgtagtgggc cagcgccctg atagacggtt tttcgccctt
     2101 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     2161 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     2221 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     2281 caattttaac attgtagaaa cgcatgtaaa acgacggcca gtgaattgta atacgactca
     2341 ctatagggtg aattccgctt aggaccttag atattgatct caa
//
