LOCUS       sequence-487940-        7317 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_026, derived from human fusion protein C11orf95_RELA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_026
FEATURES             Location/Qualifiers
     source          1..7317
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(850..1560,1561..1563,1564..1566))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(892..913)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1500..1521
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1563..1572
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1648..1851)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1677..1697)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1852..2155
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2412..2456)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2641..2874
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3234..3351
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3843..3968
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4015..4272
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4274..4653
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4684..4788
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4789..4857,4858..5649)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5007..5026)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5820..6408
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6309..6328
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6562..6579
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6647..6781
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6671..6690)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6725..6744
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6787..6982)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6801..6936
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7041..7062
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7077..7082,7083..7100,7101..7107)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7115..7131
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7120..7142
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7139..7155
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7139..7155
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca ttcaagacga cgggcaccca aaccccgaga
      601 accgccagca cgtctgctgg cgctcagacg caggcggcgg gcacgcgggg caaccggacc
      661 accacggcgt tgacgacggc acccgccacc cgctggtgct ttgcccggac ccttcggcgg
      721 gcaggccggg acgccggcgc cttcctcttc ctcttcttcc tcttcctcct cttcctcctc
      781 cgcaccttgc acgcccagac cttccgcccg ggaagcggcc gcacttcccc cggagcctcc
      841 gcttccgccc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
      901 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagcttgg actgggtgct
      961 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
     1021 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
     1081 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggctgttgt agttgtactc
     1141 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
     1201 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
     1261 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
     1321 gtgctgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
     1381 cacgagggtg ggccagggca cgggcagctt gccggtggtg cagatgaact tcagggtcag
     1441 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
     1501 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
     1561 caccatggtg gcgaccggtg gatcccgggc ccgcggtacc gtcgactgca gaattcggtt
     1621 cactaaacga gcccggttca ctaaacgagc tctgcttata tagacctccc accgtacacg
     1681 cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa agtcccgttg
     1741 attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg gaaatccccg
     1801 tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca ccatggtaat
     1861 agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg tcatgtactg
     1921 ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg tacttggcat
     1981 atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca cccattgacg
     2041 tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg acgtcaatgg
     2101 gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg taacgcggaa
     2161 ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat aactagcacg
     2221 cgttcgaagc ttgagctcgg tcccctcggg gttgggaggt gggtctgaaa cgataatggt
     2281 gaatatccct gcctaactct attcactata gaaagtacag caaaaactat tcttaaacct
     2341 accaagcctc ctactatcat tatgaataat tttatatacc acagccaatt tgttatgtta
     2401 aaccaattcc acaaacttgc ccatttatct aattccaata attcttgttc attcttttct
     2461 tgctggtttt gcgattcttc aattaaggag tgtattaagc ttgtgtaatt gttaatttct
     2521 ctgtcccact ccatccaggt cgtgtgattc caaatctgtt ccagagattt attactccaa
     2581 ctagcattcc aaggcacagc agtggtgcaa atgagttttc cagagcaacc ccaaatcccc
     2641 aggagctgtt gatcctttag gtatctttcc acagccagga ttcttgcctg gagctgcttg
     2701 atgccccaga ctgtgagttg caacagatgc tgttgcgcct caatagccct cagcaaattg
     2761 ttctgctgct gcactatacc agacaataat tgtctggcct gtaccgtcag cgtcattgac
     2821 gctgcgccca tagtgcttcc tgctgctccc aagaacccaa ggaacaaagc tcctattccc
     2881 actgctcttt tttctctctg caccactctt ctctttgcct tggtgggtgc tactcctaat
     2941 ggttcaattt ttactacttt atatttatat aattcacttc tccaattgtc cctcatatct
     3001 cctccttatg gtgttttact aaacttttcc atgttctaat cctcatcctg tctacttgcc
     3061 acacaatcat cacctgccat ctgttttcca taatccctaa tgatctttgc ttttcttctt
     3121 ggcactactt ttatgtcact attatcttgt attactactg ccccttcacc tttccagagg
     3181 agctttgctg gtcctttcca aagtggattt ctgctgtccc tgtaataaac ccgaaaattt
     3241 tgaatttttg taatttgttt ttgtaattct ttagtttgta tgtctgttgc tattatgtct
     3301 actattcttt cccctgcact gtacccccca atcccccctt ttcttttaaa attgtggatg
     3361 aatactgcca tttgtactgc tgtcttaaga tgttcagcct gatctcttac ctgtcctata
     3421 attttcttta attctttatt catagattct actactcctt gactttgggg attgtaggga
     3481 atttccaggt ctgaagatcc tgctgcttgc tgtgcttttt tcttactttt gttttgctct
     3541 tcctctatct tgtctaaagc ttccttggtg tcttttatct ctatcctttg atgcacacaa
     3601 tagagggttg ctactgtatt atataatgat ctaagttctt ctgatcctgt ctgaagggat
     3661 ggttgtagct gtcccagtat ttgtctacag ccttctgatg tttctaacag gccagtatta
     3721 actgcgaatc gttctagctc cctgcttgcc catactatat gttttaattt atattttttc
     3781 tttccccctg gccttaaccg aattttttcc catcgcgatc taattctccc ccgcttaata
     3841 ctgacgctct cgcacccatc tctctccttc tagcctccgc tagtcaaaat ttttggcgta
     3901 ctcaccagtc gccgcccctc gcctcttgcc gtgcgcgctt cagcaagccg agtcctgcgt
     3961 cgagagagct cctctggttt ccctttcgct ttcaagtccc tgttcgggcg ccactgctag
     4021 agattttcca cactgactaa aagggtctga gggatctcta gttaccagag tcacacaaca
     4081 gacgggcaca cactacttga agcactcaag gcaagcttta ttgaggctta agcagtgggt
     4141 tccctagtta gccagagagc tcccaggctc agatctggtc taaccagaga gacccagtac
     4201 aggcaaaaag cagctgctta tatgcaggat ctgagggctc gccactcccc agtcccgccc
     4261 aggccacgcc tcccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     4321 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     4381 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     4441 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     4501 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     4561 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     4621 attactatta ataactagtc aataatcaat gtcgacccag gtggcacttt tcggggaaat
     4681 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
     4741 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
     4801 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
     4861 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
     4921 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
     4981 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
     5041 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
     5101 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
     5161 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
     5221 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
     5281 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg
     5341 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
     5401 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
     5461 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
     5521 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
     5581 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
     5641 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
     5701 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
     5761 taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct
     5821 tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca
     5881 gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc
     5941 agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg ccaccacttc
     6001 aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct
     6061 gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag
     6121 gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc
     6181 tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg
     6241 agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag
     6301 cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt
     6361 gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac
     6421 gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt ctttcctgcg
     6481 ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga taccgctcgc
     6541 cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga gcgcccaata
     6601 cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagcggatc cagacatgat
     6661 aagatacatt gatgagtttg gacaaaccac aactagaatg cagtgaaaaa aatgctttat
     6721 ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt ataagctgca ataaacaagt
     6781 tagctttttg caaaagccta ggcctccaaa aaagcctcct cactacttct ggaatagctc
     6841 agaggccgag gcggcctcgg cctctgcata aataaaaaaa attagtcagc catggggcgg
     6901 agaatgggcg gaactgggcg gagttagggg cgggatgggc ggagttaggg gcgggactat
     6961 ggttgctgac taattgagat gcggatccgc tggcacgaca ggtttcccga ctggaaagcg
     7021 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     7081 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     7141 ggaaacagct atgaccatga ttacgccaag cttgggctgc aggtcgaggg atctccataa
     7201 gagaagaggg acagctatga ctgggagtag tcaggagagg aggaaaaatc tggctagtaa
     7261 aacatgtaag gaaaatttta gggatgttaa agaaaaaaat aacacaaaac aaaatat
//
