LOCUS       sequence-487958-        7542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_045, derived from human fusion protein CUX1_NUTM1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7542)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_045
FEATURES             Location/Qualifiers
     source          1..7542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(1075..1785,1786..1788,1789..1791))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1117..1138)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1466..1485
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1725..1746
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1788..1797
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1873..2076)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1902..1922)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        2077..2380
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2637..2681)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2866..3099
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3459..3576
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    4068..4193
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4240..4497
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4499..4878
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4909..5013
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5014..5082,5083..5874)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5232..5251)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6045..6633
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6534..6553
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6787..6804
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6872..7006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6896..6915)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6950..6969
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(7012..7207)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      7026..7161
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7266..7287
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7302..7307,7308..7325,7326..7332)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7340..7356
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7345..7367
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7364..7380
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7364..7380
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca cggcgacgcc agagagctgc taaagaaatc
      601 ctggctcagg cctgccgggc taaattggtg ggtaccattg gtattgctcg cctgctcccc
      661 cggattacgt ggcagctggg acggcggtgg cgccggcagg ctacccggac gacggctctc
      721 tggctggtcc ttgcccttgc gacgtgcgct gccggacagg tctgagttgg agatgcgcag
      781 agctgcgttt tcggactgca gtgaacggtt tttctccagc agcagaacct caaggggttt
      841 ggcggcgtcc tgtgtccctg caccctcgct tggcgcgaac tccatcgact tcaggatatt
      901 cagctctttt ttcacctctt cataatccgc ttggcccttc agcttttcct ctaactgctt
      961 tagggtgctg ttcttcgccg agagctgttg ttccaattga gagatttgac tcgcagagtt
     1021 ctcgcgcagc ttccgggaag cggccgcact tcccccggag cctccgcttc cgcccttgta
     1081 cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg
     1141 atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc
     1201 gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg
     1261 cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg
     1321 cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag
     1381 gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc
     1441 gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt
     1501 gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg
     1561 gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca
     1621 gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc
     1681 atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc
     1741 gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgac
     1801 cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cggttcacta aacgagcccg
     1861 gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac cgcccatttg
     1921 cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa
     1981 caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt caaaccgcta
     2041 tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga tgactaatac
     2101 gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat aatgccaggc
     2161 gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat acacttgatg
     2221 tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat ggaaagtccc
     2281 tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg ggtcgttggg
     2341 cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca tatatgggct
     2401 atgaactaat gaccccgtaa ttgattacta ttaataacta gcacgcgttc gaagcttgag
     2461 ctcggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata tccctgccta
     2521 actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa gcctcctact
     2581 atcattatga ataattttat ataccacagc caatttgtta tgttaaacca attccacaaa
     2641 cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg gttttgcgat
     2701 tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc ccactccatc
     2761 caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc attccaaggc
     2821 acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag ctgttgatcc
     2881 tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc ccagactgtg
     2941 agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg ctgctgcact
     3001 ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg
     3061 cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc tcttttttct
     3121 ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc aatttttact
     3181 actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc ttatggtgtt
     3241 ttactaaact tttccatgtt ctaatcctca tcctgtctac ttgccacaca atcatcacct
     3301 gccatctgtt ttccataatc cctaatgatc tttgcttttc ttcttggcac tacttttatg
     3361 tcactattat cttgtattac tactgcccct tcacctttcc agaggagctt tgctggtcct
     3421 ttccaaagtg gatttctgct gtccctgtaa taaacccgaa aattttgaat ttttgtaatt
     3481 tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat tctttcccct
     3541 gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac tgccatttgt
     3601 actgctgtct taagatgttc agcctgatct cttacctgtc ctataatttt ctttaattct
     3661 ttattcatag attctactac tccttgactt tggggattgt agggaatttc caggtctgaa
     3721 gatcctgctg cttgctgtgc ttttttctta cttttgtttt gctcttcctc tatcttgtct
     3781 aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact
     3841 gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc
     3901 agtatttgtc tacagccttc tgatgtttct aacaggccag tattaactgc gaatcgttct
     3961 agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt
     4021 aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac
     4081 ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc
     4141 ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct
     4201 ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg
     4261 actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta
     4321 cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag
     4381 agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct
     4441 gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccca
     4501 tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc cataaggtca
     4561 tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata gggggcgtac
     4621 ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa tactccaccc
     4681 attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc attattgacg
     4741 tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta agttatgtaa
     4801 cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac tattaataac
     4861 tagtcaataa tcaatgtcga cccaggtggc acttttcggg gaaatgtgcg cggaacccct
     4921 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     4981 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     5041 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     5101 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     5161 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     5221 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     5281 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     5341 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     5401 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     5461 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     5521 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     5581 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     5641 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     5701 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     5761 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     5821 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     5881 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     5941 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     6001 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     6061 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     6121 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     6181 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     6241 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     6301 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     6361 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     6421 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     6481 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     6541 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     6601 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     6661 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     6721 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     6781 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     6841 cccgcgcgtt ggccgattca ttaatgcagc ggatccagac atgataagat acattgatga
     6901 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     6961 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttagct ttttgcaaaa
     7021 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     7081 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     7141 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     7201 gagatgcgga tccgctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     7261 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     7321 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     7381 catgattacg ccaagcttgg gctgcaggtc gagggatctc cataagagaa gagggacagc
     7441 tatgactggg agtagtcagg agaggaggaa aaatctggct agtaaaacat gtaaggaaaa
     7501 ttttagggat gttaaagaaa aaaataacac aaaacaaaat at
//
