LOCUS       sequence-487991-        7368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged intrinsically disordered protein (IDR), 
            FOidr_084, derived from human fusion protein KMT2A_AFDN.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7368)
  AUTHORS   Maiti S, Tripathi S, Baggett DW, Phillips AH, Park CG, Wang J, 
            Wahiduzzaman, Freyaldenhoven WT, Kinger S, Pioso BJ, Bollinger JC, 
            Somjee R, Lang B, Babu MM, Kriwacki RW
  TITLE     Proteome-wide computational analyses reveal links between protein 
            condensate formation and RNA biology.
  JOURNAL   Sci Adv. 2025 Dec 5;11(49):eady1420. doi: 10.1126/sciadv.ady1420. 
            Epub 2025 Dec 3.
  PUBMED    41337585
REFERENCE   2  (bases 1 to 7368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Adv."; 
            date: "2025-12-5"; volume: "11(49):eady1420. doi"; pages: " 
            10.1126/sciadv.ady1420. Epub 2025 Dec 3"
COMMENT     Sequence Label: CL20_mEGFP_FOidr_084
FEATURES             Location/Qualifiers
     source          1..7368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     181..200
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    199..254
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     300..319
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(901..1611,1612..1614,1615..1617))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(943..964)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1292..1311
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1551..1572
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      1614..1623
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(1699..1902)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1728..1748)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1903..2206
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(2463..2507)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2692..2925
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    3285..3402
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3894..4019
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             4066..4323
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        4325..4704
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4735..4839
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4840..4908,4909..5700)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5058..5077)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5871..6459
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6360..6379
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6613..6630
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    6698..6832
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6722..6741)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6776..6795
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(6838..7033)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      6852..6987
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    7092..7113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7128..7133,7134..7151,7152..7158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7166..7182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7171..7193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7190..7206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7190..7206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aaaaaaaatc taacctcaag tcaaggcttt tctatggaat aaggaatgga cagcaggggg
       61 ctgtttcata tactgatgac ctctttatag ccaacctttg ttcatggcag ccagcatatg
      121 ggcatatgtt gccaaactct aaaccaaata ctcattctga tgttttaaat gatttgccct
      181 cccatatgtc cttccgagtg agagacacaa aaaattccaa cacactattg caatgaaaat
      241 aaatttcctt tattagccag aagtcagatg ctcaaggggc ttcatgatgt ccccataatt
      301 tttggcagag ggaaaaagat ctgctagctc gagcagctga agctttattg aggcttaagc
      361 agtgggttcc ctagttagcc agagagctcc caggctcaga tctggtctaa ccagagagac
      421 ccagtacagg caaaaagcag atcttgtctt ctttgggagt gaattagccc ttccagtccc
      481 cccttttctt ttaaaaagtg gctaagatct acagctgcct tgtaagtcat tggtcttaaa
      541 ggtaccgtaa tcttatctag aaccggttca caggatgagt tccggaccgg aggcgtcctg
      601 cgtcctggat tcttggcgtc ggtagtccgg ctgttgttca acacgaatca tcggtttaac
      661 cataccacgt tccgccgtag acgaagcaga ggaaacacgg tcagagtcta atcgagtggt
      721 gcttttgctg gtcggcagcg ctgtaccgct gtggatgtcg ccacccagat caaaggtggt
      781 ttcctgtaca atccccggtt tgtgacgcgg gcctttaacc atcagcccac cgtccacact
      841 acgcttggcc agagcatgcc gggaagcggc cgcacttccc ccggagcctc cgcttccgcc
      901 cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact ccagcaggac
      961 catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc tcaggtagtg
     1021 gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc
     1081 gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct tgatgccgtt
     1141 cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact ccagcttgtg
     1201 ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc ggttcaccag
     1261 ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa
     1321 gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt cgtgctgctt
     1381 catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg tcacgagggt
     1441 gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca gcttgccgta
     1501 ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta cgtcgccgtc
     1561 cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc tcaccatggt
     1621 ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt tcactaaacg
     1681 agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     1741 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     1801 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     1861 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     1921 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     1981 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     2041 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     2101 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     2161 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     2221 tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac gcgttcgaag
     2281 cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg tgaatatccc
     2341 tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc taccaagcct
     2401 cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt aaaccaattc
     2461 cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc ttgctggttt
     2521 tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc tctgtcccac
     2581 tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca actagcattc
     2641 caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc caggagctgt
     2701 tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt gatgccccag
     2761 actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc
     2821 tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc
     2881 atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc cactgctctt
     2941 ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa tggttcaatt
     3001 tttactactt tatatttata taattcactt ctccaattgt ccctcatatc tcctccttat
     3061 ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc cacacaatca
     3121 tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct tggcactact
     3181 tttatgtcac tattatcttg tattactact gccccttcac ctttccagag gagctttgct
     3241 ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt
     3301 gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc tactattctt
     3361 tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat gaatactgcc
     3421 atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat aattttcttt
     3481 aattctttat tcatagattc tactactcct tgactttggg gattgtaggg aatttccagg
     3541 tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc ttcctctatc
     3601 ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca atagagggtt
     3661 gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga tggttgtagc
     3721 tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt aactgcgaat
     3781 cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt ctttccccct
     3841 ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat actgacgctc
     3901 tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt actcaccagt
     3961 cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc
     4021 tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta gagattttcc
     4081 acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac agacgggcac
     4141 acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg ttccctagtt
     4201 agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta caggcaaaaa
     4261 gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc caggccacgc
     4321 ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata
     4381 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg
     4441 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact
     4501 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta
     4561 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt
     4621 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt
     4681 aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga
     4741 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4801 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4861 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4921 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4981 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     5041 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5101 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5161 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5221 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5281 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5341 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5401 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5461 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5521 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5581 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5641 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5701 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5761 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5821 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5881 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5941 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     6001 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6061 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6121 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6181 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6241 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6301 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6361 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6421 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6481 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6541 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6601 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6661 cctctccccg cgcgttggcc gattcattaa tgcagcggat ccagacatga taagatacat
     6721 tgatgagttt ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat
     6781 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttagcttttt
     6841 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     6901 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     6961 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     7021 ctaattgaga tgcggatccg ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7081 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7141 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7201 tatgaccatg attacgccaa gcttgggctg caggtcgagg gatctccata agagaagagg
     7261 gacagctatg actgggagta gtcaggagag gaggaaaaat ctggctagta aaacatgtaa
     7321 ggaaaatttt agggatgtta aagaaaaaaa taacacaaaa caaaatat
//
