LOCUS       sequence-488003-        6471 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses human XPO1 protein with an N-terminal cMyc tag. The 
            V518E_5561H mutations disrupt ASB8 binding..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6471)
  AUTHORS   Wing CE, Fung HYJ, Kwanten B, Cagatay T, Niesman AB, Jacquemyn M, 
            Gharghabi M, Permentier B, Shakya B, Nandi R, Ready JM, Kashyap T, 
            Shacham S, Landesman Y, Lapalombella R, Daelemans D, Chook YM
  TITLE     SINE compounds activate exportin 1 degradation through an allosteric
            mechanism.
  JOURNAL   Nat Chem Biol. 2025 Dec;21(12):2002-2013. doi: 
            10.1038/s41589-025-02058-0. Epub 2025 Nov 21.
  PUBMED    41286136
REFERENCE   2  (bases 1 to 6471)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41589-025-02058-0"; journalName: "Nat Chem Biol"; date: 
            "2025-12"; volume: "21"; issue: "12"; pages: "2002-2013"
COMMENT     Sequence Label: pGL4-CMV-myc-XPO1_V518E_F561H
FEATURES             Location/Qualifiers
     source          1..6471
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    1..49
                     /note="synthetic polyadenylation signal"
     misc_feature    63..154
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     103..122
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
     enhancer        221..600
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        601..804
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     755..775
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     849..868
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        849..867
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             913..942
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    4179..4300
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4190..4209)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4244..4263
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(4548..4565)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4719..5307)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4799..4818)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5507..6298,6299..6367))
                     /codon_start=1
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 aataaaatat ctttattttc attacatctg tgtgttggtt ttttgtgtga atcgatagta
       61 ctaacatacg ctctccatca aaacaaaacg aaacaaaaca aactagcaaa ataggctgtc
      121 cccagtgcaa gtgcaggtgc cagaacattt ctcttaatga tagctagcgg gccagtaccc
      181 aaaaagcggg gggtctggtg gcagtggagg gggatccgtt gacattgatt attgactagt
      241 tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt
      301 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
      361 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      421 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      481 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      541 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      601 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      661 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      721 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      781 tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc ttactggctt
      841 atcgaaatta atacgactca ctatagggag acccaagctg gctagcgttt aaacttaagc
      901 ttggtaccaa tggaacagaa gctgatctcc gaggaggacc ttccagcaat tatgacaatg
      961 ttagcagacc atgcagctcg tcagctgctt gatttcagcc aaaaactgga tatcaactta
     1021 ttagataatg tggtgaattg cttataccat ggagaaggag cccagcaaag aatggctcaa
     1081 gaagtactga cacatttaaa ggagcatcct gatgcttgga caagagtcga cacaattttg
     1141 gaattttctc agaatatgaa tacgaaatac tatggactac aaattttgga aaatgtgata
     1201 aaaacaaggt ggaagattct tccaaggaac cagtgcgaag gaataaaaaa atacgttgtt
     1261 ggcctcatta tcaagacgtc atctgaccca acttgtgtag agaaagaaaa ggtgtatatc
     1321 ggaaaattaa atatgatcct tgttcagata ctgaaacaag aatggcccaa acattggcca
     1381 acttttatca gtgatattgt tggagcaagt aggaccagcg aaagtctctg tcaaaataat
     1441 atggtgattc ttaaactctt gagtgaagaa gtatttgatt tctctagtgg acagataacc
     1501 caagtcaaat ctaagcattt aaaagacagc atgtgcaatg aattctcaca gatatttcaa
     1561 ctgtgtcagt ttgtaatgga aaattctcaa aatgctccac ttgtacatgc aaccttggaa
     1621 acattgctca gatttctgaa ctggattccc ctgggatata tttttgagac caaattaatc
     1681 agcacattga tttataagtt cctgaatgtt ccaatgtttc gaaatgtctc tctgaagtgc
     1741 ctcactgaga ttgctggtgt gagtgtaagc caatatgaag aacaatttgt aacactattt
     1801 actctgacaa tgatgcaact aaagcagatg cttcctttaa ataccaatat tcgacttgcg
     1861 tactcaaatg gaaaagatga tgaacagaac ttcattcaaa atctcagttt gtttctctgc
     1921 acctttctta aggaacatga tcaacttata gaaaaaagat taaatctcag ggaaactctt
     1981 atggaggccc ttcattatat gttgttggta tctgaagtag aagaaactga aatctttaaa
     2041 atttgtcttg aatactggaa tcatttggct gctgaactct atagagagag tccattctct
     2101 acatctgcct ctccgttgct ttctggaagt caacattttg atgttcctcc caggagacag
     2161 ctatatttgc ccatgttatt caaggtccgt ttattaatgg ttagtcgaat ggctaaacca
     2221 gaggaagtat tggttgtaga gaatgatcaa ggagaagttg tgagagaatt catgaaggat
     2281 acagattcca taaatttgta taagaatatg agggaaacat tggtttatct tactcatctg
     2341 gattatgtag atacagaaag aataatgaca gagaagcttc acaatcaagt gaatggtaca
     2401 gagtggtcat ggaaaaattt gaatacattg tgttgggcaa taggctccat tagtggagca
     2461 atgcatgaag aggacgaaaa acgatttctt gagactgtta taaaggatct attaggatta
     2521 tgtgaacaga aaagaggcaa agataataaa gctattattg catcaaatat catgtacata
     2581 gtaggtcaat acccacgttt tttgagagct cactggaaac acctgaagac tgtagttaac
     2641 aagctgttcg aattcatgca tgagacccat gatggagtcc aggatatggc ttgtgatact
     2701 ttcattaaaa tagcccaaaa atgccgcagg catttcgttc aggttcaggt tggagaagtg
     2761 atgccattta ttgatgaaat tttgaacaac attaacacta ttatttgtga tcttcagcct
     2821 caacaggttc atacgtttta tgaagctgtg gggtacatga ttggtgcaca aacagatcaa
     2881 acagtacaag aacacttgat agaaaagtac atgttactcc ctaatcaagt gtgggatagt
     2941 ataatccagc aggcaaccaa aaatgtggat atactgaaag atcctgaaac agtcaagcag
     3001 cttggtagca ttttgaaaac aaatgtgaga gcctgcaaag ctgttggaca cccctttgta
     3061 attcagcttg gaagaattta tttagatatg cttaatgtat acaagtgcct cagtgaaaat
     3121 atttctgcag ctatccaagc taatggtgaa atggttacaa agcaaccatt gattagaagt
     3181 atgcgaactg taaaaaggga aactttaaag ttaatatctg gttgggtgag ccgatccaat
     3241 gatccacaga tggtcgctga aaattttgtt ccccctctgt tggatgcagt tctcattgat
     3301 tatcagagaa atgtcccagc tgctagagaa ccagaagtgc ttagtactat ggccataatt
     3361 gtcaacaagt tagggggaca tataacagct gaaatacctc aaatatttga tgctgttttt
     3421 gaatgcacat tgaatatgat aaataaggac tttgaagaat atcctgagca tagaacgaac
     3481 tttttcttac tacttcaggc tgtcaattct cattgtttcc cagcattcct tgctattcca
     3541 cctacacagt ttaaacttgt tttggattcc atcatttggg ctttcaaaca tactatgagg
     3601 aatgtcgcag atacgggctt acagatactt tttacactct tacaaaatgt tgcacaagaa
     3661 gaagctgcag ctcagagttt ttatcaaact tatttttgtg atattctcca gcatatcttt
     3721 tctgttgtga cagacacttc acatactgct ggtttaacaa tgcatgcatc aattcttgca
     3781 tatatgttta atttggttga agaaggaaaa ataagtacat cattaaatcc tggaaatcca
     3841 gttaacaacc aaatctttct tcaggaatat gtggctaatc tccttaagtc ggccttccct
     3901 cacctacaag atgctcaagt aaagctcttt gtgacagggc ttttcagctt aaatcaagat
     3961 attcctgctt tcaaggaaca tttaagagat ttcctagttc aaataaagga atttgcaggt
     4021 gaagacactt ctgatttgtt tttggaagag agagaaatag ccctacggca ggctgatgaa
     4081 gagaaacata aacgtcaaat gtctgtccct ggcatcttta atccacatga gattccagaa
     4141 gaaatgtgtg attaagggcc cgcttcgagc agacatgata agatacattg atgagtttgg
     4201 acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat
     4261 tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca
     4321 ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt aaaacctcta
     4381 caaatgtggt aaaatcgata aagatccgtc gaccgatgcc cttgagagcc ttcaacccag
     4441 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct
     4501 ttatcatgca actcgtagga caggtgccgg cagcgctctt ccgcttcctc gctcactgac
     4561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     4621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     4921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     4981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5461 taaacttggt ctgacagcgg ccgcaaatgc taaaccactg cagtggttac cagtgcttga
     5521 tcagtgaggc accgatctca gcgatctgcc tatttcgttc gtccatagtg gcctgactcc
     5581 ccgtcgtgta gatcactacg attcgtgagg gcttaccatc aggccccagc gcagcaatga
     5641 tgccgcgaga gccgcgttca ccggcccccg atttgtcagc aatgaaccag ccagcaggga
     5701 gggccgagcg aagaagtggt cctgctactt tgtccgcctc catccagtct atgagctgct
     5761 gtcgtgatgc tagagtaaga agttcgccag tgagtagttt ccgaagagtt gtggccattg
     5821 ctactggcat cgtggtatca cgctcgtcgt tcggtatggc ttcgttcaac tctggttccc
     5881 agcggtcaag ccgggtcaca tgatcaccca tattatgaag aaatgcagtc agctccttag
     5941 ggcctccgat cgttgtcaga agtaagttgg ccgcggtgtt gtcgctcatg gtaatggcag
     6001 cactacacaa ttctcttacc gtcatgccat ccgtaagatg cttttccgtg accggcgagt
     6061 actcaaccaa gtcgttttgt gagtagtgta tacggcgacc aagctgctct tgcccggcgt
     6121 ctatacggga caacaccgcg ccacatagca gtactttgaa agtgctcatc atcgggaatc
     6181 gttcttcggg gcggaaagac tcaaggatct tgccgctatt gagatccagt tcgatatagc
     6241 ccactcttgc acccagttga tcttcagcat cttttacttt caccagcgtt tcggggtgtg
     6301 caaaaacagg caagcaaaat gccgcaaaga agggaatgag tgcgacacga aaatgttgga
     6361 tgctcatact cgtccttttt caatattatt gaagcattta tcagggttac tagtacgtct
     6421 ctcaaggata agtaagtaat attaaggtac gggaggtatt ggacaggccg c
//
