LOCUS       sequence-488020-       10196 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express CNOT3-R59S-Tev-3xFlag in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10196)
  AUTHORS   Zhu X, Cruz VE, Zhang H, Erzberger JP, Mendell JT
  TITLE     Specific tRNAs promote mRNA decay by recruiting the CCR4-NOT complex
            to translating ribosomes.
  JOURNAL   Science. 2024 Nov 22;386(6724):eadq8587. doi: 
            10.1126/science.adq8587. Epub 2024 Nov 22.
  PUBMED    39571015
REFERENCE   2  (bases 1 to 10196)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2024-11-22"; volume: "386(6724):eadq8587. doi"; pages: " 
            10.1126/science.adq8587. Epub 2024 Nov 22"
COMMENT     Sequence Label: Lenti-CMV-CNOT3-R59S-Tev-3xFlag
FEATURES             Location/Qualifiers
     source          1..10196
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     5..22
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    139..160
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(175..180,181..198,199..205)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    213..229
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     218..240
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     237..253
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     237..253
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     272..292
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        274..292
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        320..546
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             547..727
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    774..899
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1392..1625
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1810..1854
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2121..2237
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2260..2563
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2564..2767
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2718..2738
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2764..2788
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2862..2871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5136..5156
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             5157..5222
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     protein_bind    complement(5289..5313)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5344..5932
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5397..5417)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5815..5826)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5983..6003)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5988..6317
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6168..6303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             6345..6944
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6345..6364)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6841..6861
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7034..7267
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7339..7460
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7376..7395)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7430..7449
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7500..7635
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7655..7674)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7656..7674)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7684..7701)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7684..7700)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7693..7715)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      7842..8297
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7929..7948)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8139..8160
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8323..8427
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8428..8496,8497..9288)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8646..8665)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9459..10047
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9948..9967
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
       61 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      121 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      181 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      241 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
      301 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
      361 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      421 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      481 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      541 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      601 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      661 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      721 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      781 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
      841 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
      901 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
      961 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1021 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1081 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1141 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1201 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1261 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1321 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1381 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1441 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1501 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1561 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1621 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1681 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1741 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1801 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     1861 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     1921 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     1981 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     2041 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     2101 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2161 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2221 acaaaattca aaattttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2281 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2341 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2401 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2461 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2521 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2581 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2641 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2701 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2761 cagagctcgt ttagtgaacc gtcagatcgc ctgtcgcgat acgctcctca gcggcggcgc
     2821 cagctcctgt gcgtccgtct ccaagagagt agagtgcgtc tgccaccatg gcggacaagc
     2881 gcaaactcca aggtgagatt gatcgctgcc tcaagaaggt gtccgagggc gtggagcagt
     2941 ttgaagatat ttggcagaag ctccacaatg cagccaacgc gaaccagaaa gaaaagtatg
     3001 aggctgacct aaagaaggag attaagaagc tacaacggct gtcggaccaa atcaagacat
     3061 gggtagcgtc caacgagatc aaggacaaga ggcagcttat agacaaccgc aagctcattg
     3121 agacgcaaat ggaacggttc aaagttgtgg aacgagagac caaaaccaaa gcttacagca
     3181 aagagggcct gggcctggcc cagaaggtag atcctgccca gaaggagaag gaagaggttg
     3241 gccagtggct cacgaatacc atcgacacgc tcaacatgca ggtggaccag tttgagagtg
     3301 aagtggagtc actgtcagtg cagacacgca agaagaaggg cgacaaggat aagcaggacc
     3361 ggattgaggg cttgaagcgg cacatcgaga agcaccgcta ccacgtgcgc atgctagaga
     3421 ccatcctgcg catgctggac aatgactcca ttctcgttga cgccatccgc aagatcaagg
     3481 acgacgttga gtactatgtt gactcatccc aggaccccga cttcgaggag aacgagtttc
     3541 tctacgatga cctggacctc gaggacattc cacaggcgct ggtcgccacc tcccccccca
     3601 gccacagcca catggaggat gagatcttca accagtccag cagcacgccc acctcaacca
     3661 cctccagctc tcccatcccg cccagcccag ccaactgtac cacggaaaac tctgaagatg
     3721 ataagaagag gggacgttcc acagacagtg aagtcagcca gtctccagcc aaaaacggct
     3781 ccaagcctgt ccacagcaac cagcaccctc agtccccagc tgtgccgccc acctacccct
     3841 ccggcccccc gcctgctgcc tctgccttga gcaccactcc tggcaacaat ggggtccccg
     3901 cccccgcagc acccccaagt gccctgggcc ccaaggccag tccagctccc agccacaact
     3961 cgggcacccc tgctccctat gcccaggcgg tggccccacc agctcccagt gggcccagca
     4021 cgacccagcc ccggcccccc agcgtccagc ctagcggagg cggaggcggc ggcagcggag
     4081 gcggagggag cagcagcagt agtaacagca gtgccggtgg aggggctggc aagcagaatg
     4141 gcgccaccag ttacagctca gttgtggcag acagcccggc agaggtggct ttgagcagca
     4201 gtgggggcaa caatgccagc agccaggcct tgggcccccc ttccggcccc cacaacccac
     4261 ctcccagcac ctcgaaggaa cccagtgcgg cagccccaac gggggctggg ggcgtggccc
     4321 caggctcagg gaacaactca gggggaccca gcctcctggt gccactgcct gtgaatcctc
     4381 ccagctcccc aacgcccagc ttcagtgatg ccaaggcagc cggtgccctg ctcaatgggc
     4441 ctccacagtt cagcaccgcc ccagaaatca aggcccctga gcctctgagc tccttgaagt
     4501 ccatggcgga acgggcagcc atcagctctg gcattgagga ccctgtgcca acgctgcacc
     4561 tgaccgagcg agacatcatc ctgagcagta catcagcacc tccggcctca gcccagccgc
     4621 ccctgcagct gtcagaggtg aacataccgc tgtcgctggg tgtctgtcca ctgggccctg
     4681 tgcccctcac caaggagcag ctctatcagc aggccatgga agaggccgcc tggcaccaca
     4741 tgcctcaccc ctctgactct gagcgtattc ggcagtacct cccccggaac ccctgtccga
     4801 cgccccccta ccaccaccag atgccacccc cacactcgga cactgtggaa ttctaccagc
     4861 gcctgtcgac cgagacactc ttcttcatct tctactatct ggagggcact aaggcacagt
     4921 atctggcagc caaggcccta aagaagcagt catggcgatt ccacaccaag tacatgatgt
     4981 ggttccagag gcacgaggag cccaagacca tcactgacga gtttgagcag ggcacctaca
     5041 tctactttga ctacgagaag tggggccagc ggaagaagga aggcttcacc tttgagtacc
     5101 gctacctgga ggaccgggac ctccaggatc tgtttgagaa cctgtacttc cagggcgact
     5161 acaaagacca tgacggtgat tataaagatc atgacatcga ttacaaggat gacgatgaca
     5221 agtagaaaca ctagtagacc acctccccga tccggtaccg aattcgcggc cgcactcgag
     5281 atatctagac ccagctttct tgtacaaagt ggttgatatc cagcacagtg gcggccgctc
     5341 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5401 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5461 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5521 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5581 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5641 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5701 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5761 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5821 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     5881 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctggaatt
     5941 ctgcagatat ccggttagta atgagtttgg aattaattct gtggaatgtg tgtcagttag
     6001 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     6061 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     6121 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     6181 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     6241 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     6301 gcctaggctt ttgcaaaaag ctcccggatc caccggagct taccatgacc gagtacaagc
     6361 ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg
     6421 cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg
     6481 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
     6541 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
     6601 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
     6661 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
     6721 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
     6781 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
     6841 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
     6901 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgataagca caattcgagc
     6961 tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
     7021 gaaaaggggg gactggaagg gctagctcac tcccaacgaa gacaagatct gctttttgct
     7081 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7141 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7201 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7261 tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga
     7321 atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     7381 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     7441 aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca
     7501 tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt
     7561 ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag
     7621 gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg
     7681 ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
     7741 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
     7801 tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc
     7861 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     7921 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     7981 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     8041 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     8101 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     8161 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc
     8221 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     8281 attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8341 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8401 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8461 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8521 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8581 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8641 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8701 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8761 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8821 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8881 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8941 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     9001 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     9061 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9121 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9181 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9241 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9301 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9361 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9421 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     9481 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     9541 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     9601 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     9661 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     9721 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     9781 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     9841 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     9901 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     9961 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
    10021 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
    10081 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
    10141 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccga
//
