LOCUS       sequence-488024-       14039 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Cas12a CRISPRko positive control; targets CD46, CD47, CD55.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14039)
  TITLE     Doench and Root CRISPR plasmids 2025
REFERENCE   2  (bases 1 to 14039)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pMV_AA097
FEATURES             Location/Qualifiers
     source          1..14039
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     772..792
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        complement(2819..3067)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(2877..2896)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(3047..3067)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     promoter        3141..4319
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3372..4310
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4267..4287
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4324..4333
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4369..4389
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4396..8313
                     /codon_start=1
                     /product="CRISPR-associated protein from Acidaminococcus 
                     sp. BV3L6 (Zetsche et al., 2015)"
                     /label=AsCpf1
                     /translation="TQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARNDH
                     YKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRNA
                     IHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFDK
                     FTTYFSGFYRNRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHFE
                     NVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVLN
                     LAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLLR
                     NENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTGK
                     ITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTLK
                     KQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKAR
                     NYATKKPYSVEKFKLNFQMPTLARGWDVNREKNNGAILFVKNGLYYLGIMPKQKGRYKA
                     LSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEPL
                     EITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSIDL
                     SSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKGH
                     HGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLKD
                     QKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFFH
                     VPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQRS
                     LNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAVV
                     VLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQF
                     TSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYDV
                     KTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIENH
                     RFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQMR
                     NSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKESK
                     DLKLQNGISNQDWLAYIQELRN"
     regulatory      8033..8042
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8320..8367
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
     CDS             8374..8454
                     /codon_start=1
                     /product="three tandem HA epitope tags"
                     /label=3xHA
                     /translation="YPYDVPDYAYPYDVPDYAYPYDVPDYA"
     CDS             8461..8517
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             8518..9111
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     9011..9031
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9131..9719
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9184..9204)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9602..9613)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9791..10024
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(10050..10067)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10056..10280
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      10326..10754
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10413..10432)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10623..10644
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(10763..10783)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10768..11097
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10948..11083
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     11010..11029
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        11145..11192
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             11211..11585
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    11715..11836
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11752..11771)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11806..11825
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(11885..11901)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11885..11901)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(11898..11920)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    11909..11925
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(11933..11939,11940..11957,11958..11963))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11978..11999
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(12116..12133)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12287..12875)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12367..12386)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13046..13837,13838..13906))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13669..13688
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13907..14011)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta aggtaccggg cagaaccgac tcggtgccac tttttcaaaa
     2641 aaaaccttac cgtccccagc aggcactatc tactagagta gaaatttagt gccgtccagg
     2701 ttacagaaga atctacgata gtagaaatta gtagctgagc tgatcctgga aagatctacg
     2761 acagtagaaa ttagtctgtg acagtaacag tacttgatct acaagagtag aaattaccgg
     2821 tgtttcgtcc tttccacaag atatataaag ccaagaaatc gaaatacttt caagttacgg
     2881 taagcatatg atagtccatt ttaaaacata attttaaaac tgcaaactac ccaagaaatt
     2941 attactttct acgtcacgta ttttgtacta atatctttgt gtttacagtc aaattaattc
     3001 taattatctc tctaacagcc ttgtatcgta tatgcaaata tgaaggaatc atgggaaata
     3061 ggccctcttc gaatctagtt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     3121 agctaatgga ccttctacac ggctccggtg cccgtcagtg ggcagagcgc acatcgccca
     3181 cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc
     3241 gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg
     3301 gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg
     3361 ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg
     3421 gcccttgcgt gccttgaatt acttccacct ggctgcagta cgtgattctt gatcccgagc
     3481 ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc
     3541 gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc
     3601 ttcgcgcctg tctcgctgct ttcgataagt ctctagccat ttaaaatttt tgatgacctg
     3661 ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc gggccaagat ctgcacactg
     3721 gtatttcggt ttttggggcc gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt
     3781 cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg acgggggtag tctcaagctg
     3841 gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa
     3901 ggctggcccg gtcggcacca gttgcgtgag cggaaagatg gccgcttccc ggccctgctg
     3961 cagggagctc aaaatggagg acgcggcgct cgggagagcg ggcgggtgag tcacccacac
     4021 aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg tgactccacg gagtaccggg
     4081 cgccgtccag gcacctcgat tagttctcga gcttttggag tacgtcgtct ttaggttggg
     4141 gggaggggtt ttatgcgatg gagtttcccc acactgagtg ggtggagact gaagttaggc
     4201 cagcttggca cttgatgtaa ttctccttgg aatttgccct ttttgagttt ggatcttggt
     4261 tcattctcaa gcctcagaca gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa
     4321 ggagccacca tggccaaacg gacagccgac ggaagcgagt tcgagtcacc aaagaagaag
     4381 cggaaagtcg gcagcacaca gttcgagggc tttaccaacc tgtatcaggt gagcaagaca
     4441 ctgcggtttg agctgatccc acagggcaag accctgaagc acatccagga gcagggcttc
     4501 atcgaggagg acaaggcccg caatgatcac tacaaggagc tgaagcccat catcgatcgg
     4561 atctacaaga cctatgccga ccagtgcctg cagctggtgc agctggattg ggagaacctg
     4621 agcgccgcca tcgactccta tagaaaggag aaaaccgagg agacaaggaa cgccctgatc
     4681 gaggagcagg ccacatatcg caatgccatc cacgactact tcatcggccg gacagacaac
     4741 ctgaccgatg ccatcaataa gagacacgcc gagatctaca agggcctgtt caaggccgag
     4801 ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga ccacaaccga gcacgagaac
     4861 gccctgctgc ggagcttcga caagtttaca acctacttct ccggctttta tcggaacagg
     4921 aagaacgtgt tcagcgccga ggatatcagc acagccatcc cacaccgcat cgtgcaggac
     4981 aacttcccca agtttaagga gaattgtcac atcttcacac gcctgatcac cgccgtgccc
     5041 agcctgcggg agcactttga gaacgtgaag aaggccatcg gcatcttcgt gagcacctcc
     5101 atcgaggagg tgttttcctt ccctttttat aaccagctgc tgacacagac ccagatcgac
     5161 ctgtataacc agctgctggg aggaatctct cgggaggcag gcaccgagaa gatcaagggc
     5221 ctgaacgagg tgctgaatct ggccatccag aagaatgatg agacagccca catcatcgcc
     5281 tccctgccac acagattcat ccccctgttt aagcagatcc tgtccgatag gaacaccctg
     5341 tctttcatcc tggaggagtt taagagcgac gaggaagtga tccagtcctt ctgcaagtac
     5401 aagacactgc tgagaaacga gaacgtgctg gagacagccg aggccctgtt taacgagctg
     5461 aacagcatcg acctgacaca catcttcatc agccacaaga agctggagac aatcagcagc
     5521 gccctgtgcg accactggga tacactgagg aatgccctgt atgagcggag aatctccgag
     5581 ctgacaggca agatcaccaa gtctgccaag gagaaggtgc agcgcagcct gaagcacgag
     5641 gatatcaacc tgcaggagat catctctgcc gcaggcaagg agctgagcga ggccttcaag
     5701 cagaaaacca gcgagatcct gtcccacgca cacgccgccc tggatcagcc actgcctaca
     5761 accctgaaga agcaggagga gaaggagatc ctgaagtctc agctggacag cctgctgggc
     5821 ctgtaccacc tgctggactg gtttgccgtg gatgagtcca acgaggtgga ccccgagttc
     5881 tctgcccggc tgaccggcat caagctggag atggagcctt ctctgagctt ctacaacaag
     5941 gccagaaatt atgccaccaa gaagccctac tccgtggaga agttcaagct gaactttcag
     6001 atgcctacac tggcccgcgg ctgggacgtg aatcgggaga agaacaatgg cgccatcctg
     6061 tttgtgaaga acggcctgta ctatctgggc atcatgccaa agcagaaggg caggtataag
     6121 gccctgagct tcgagcccac agagaaaacc agcgagggct ttgataagat gtactatgac
     6181 tacttccctg atgccgccaa gatgatccca aagtgcagca cccagctgaa ggccgtgaca
     6241 gcccactttc agacccacac aacccccatc ctgctgtcca acaatttcat cgagcctctg
     6301 gagatcacaa aggagatcta cgacctgaac aatcctgaga aggagccaaa gaagtttcag
     6361 acagcctacg ccaagaaaac cggcgaccag aagggctaca gagaggccct gtgcaagtgg
     6421 atcgacttca caagggattt tctgtccaag tataccaaga caacctctat cgatctgtct
     6481 agcctgcggc catcctctca gtataaggac ctgggcgagt actatgccga gctgaatccc
     6541 ctgctgtacc acatcagctt ccagagaatc gccgagaagg agatcatgga tgccgtggag
     6601 acaggcaagc tgtacctgtt ccagatctat aacaaggact ttgccaaggg ccaccacggc
     6661 aagcctaatc tgcacacact gtattggacc ggcctgtttt ctccagagaa cctggccaag
     6721 acaagcatca agctgaatgg ccaggccgag ctgttctacc gccctaagtc caggatgaag
     6781 aggatggcac accggctggg agagaagatg ctgaacaaga agctgaagga tcagaaaacc
     6841 ccaatccccg acaccctgta ccaggagctg tacgactatg tgaatcacag actgtcccac
     6901 gacctgtctg atgaggccag ggccctgctg cccaacgtga tcaccaagga ggtgtctcac
     6961 gagatcatca aggataggcg ctttaccagc gacaagttct ttttccacgt gcctatcaca
     7021 ctgaactatc aggccgccaa ttccccatct aagttcaacc agagggtgaa tgcctacctg
     7081 aaggagcacc ccgagacacc tatcatcggc atcgatcggg gcgagagaaa cctgatctat
     7141 atcacagtga tcgactccac cggcaagatc ctggagcagc ggagcctgaa caccatccag
     7201 cagtttgatt accagaagaa gctggacaac agggagaagg agagggtggc agcaaggcag
     7261 gcctggtctg tggtgggcac aatcaaggat ctgaagcagg gctatctgag ccaggtcatc
     7321 cacgagatcg tggacctgat gatccactac caggccgtgg tggtgctgga gaacctgaat
     7381 ttcggcttta agagcaagag gaccggcatc gccgagaagg ccgtgtacca gcagttcgag
     7441 aagatgctga tcgataagct gaattgcctg gtgctgaagg actatccagc agagaaagtg
     7501 ggaggcgtgc tgaacccata ccagctgaca gaccagttca cctcctttgc caagatgggc
     7561 acccagtctg gcttcctgtt ttacgtgcct gccccatata catctaagat cgatcccctg
     7621 accggcttcg tggacccctt cgtgtggaaa accatcaaga atcacgagag ccgcaagcac
     7681 ttcctggagg gcttcgactt tctgcactac gacgtgaaaa ccggcgactt catcctgcac
     7741 tttaagatga acagaaatct gtccttccag aggggcctgc ccggctttat gcctgcatgg
     7801 gatatcgtgt tcgagaagaa cgagacacag tttgacgcca agggcacccc tttcatcgcc
     7861 ggcaagagaa tcgtgccagt gatcgagaat cacagattca ccggcagata ccgggacctg
     7921 tatcctgcca acgagctgat cgccctgctg gaggagaagg gcatcgtgtt cagggatggc
     7981 tccaacatcc tgccaaagct gctggagaat gacgattctc acgccatcga caccatggtg
     8041 gccctgatcc gcagcgtgct gcagatgcgg aactccaatg ccgccacagg cgaggactat
     8101 atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg actcccggtt tcagaaccca
     8161 gagtggccca tggacgccga tgccaatggc gcctaccaca tcgccctgaa gggccagctg
     8221 ctgctgaatc acctgaagga gagcaaggat ctgaagctgc agaacggcat ctccaatcag
     8281 gactggctgg cctacatcca ggagctgcgc aacgggtcca aaaggccggc ggccacgaaa
     8341 aaggccggcc aggcaaaaaa gaaaaaggga tcctacccat acgatgttcc agattacgct
     8401 tatccctacg acgtgcctga ttatgcatac ccatatgatg tccccgacta tgccggaagc
     8461 gcaacaaact tctctctgct gaaacaagcc ggagatgtcg aagagaatcc tggaccgacc
     8521 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     8581 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     8641 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     8701 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     8761 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     8821 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     8881 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     8941 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     9001 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     9061 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc cggatcctga
     9121 taaattcgaa aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     9181 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     9241 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     9301 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     9361 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     9421 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     9481 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     9541 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     9601 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     9661 tccgcgtcct cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     9721 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     9781 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     9841 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     9901 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     9961 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
    10021 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
    10081 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
    10141 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
    10201 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gataatagca ggcatgctgg
    10261 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
    10321 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    10381 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    10441 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    10501 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    10561 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    10621 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    10681 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    10741 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
    10801 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
    10861 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    10921 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
    10981 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
    11041 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
    11101 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
    11161 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
    11221 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
    11281 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
    11341 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
    11401 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
    11461 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
    11521 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
    11581 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
    11641 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
    11701 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    11761 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    11821 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    11881 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    11941 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    12001 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    12061 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    12121 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    12181 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    12241 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    12301 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    12361 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    12421 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    12481 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    12541 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    12601 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    12661 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    12721 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    12781 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    12841 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    12901 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    12961 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    13021 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    13081 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    13141 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    13201 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    13261 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    13321 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    13381 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    13441 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    13501 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    13561 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    13621 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    13681 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    13741 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    13801 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    13861 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    13921 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    13981 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgac
//
