LOCUS       sequence-48806-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Camp ND, James RG, Dawson DW, Yan F, Davison JM, Houck SA, Tang X, 
            Zheng N, Major MB, Moon RT
  TITLE     Wilms tumor gene on X chromosome (WTX) inhibits degradation of NRF2 
            protein through competitive binding to KEAP1 protein.
  JOURNAL   J Biol Chem. 2012 Feb 24;287(9):6539-50. Epub 2012 Jan 3.
  PUBMED    22215675
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2012-02-24"; volume: "287(9)"; pages: "6539-50. Epub 
            2012 Jan 3"
COMMENT     Sequence Label: NC15 pGLUE CUL3
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      100..109
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             106..219
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             238..258
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             265..291
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             292..369
                     /codon_start=1
                     /product="calmodulin-binding peptide"
                     /label=CBP
                     /note="derived from skeletal muscle myosin light chain 
                     kinase; binds calmodulin with nanomolar affinity in the 
                     presence of calcium"
                     /translation="KRRWKKNFIAVSAANRFKKISSSGAL"
ORIGIN
        1 ttggctactg agaaccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct tggtaccgag ctcggatcga tttaattaag ccaccatgga cgagaagacc
      121 accggctggc ggggcggcca cgtggtggag ggcctggccg gcgagctgga gcagctgcgg
      181 gcccggctgg agcaccaccc ccagggccag cgggagcccg gcggaagccc cggtggcgag
      241 aacctgtact tccagggcgc aagctacccc tacgacgtgc ccgactacgc caagcggcgg
      301 tggaagaaga acttcatcgc cgtgagcgcc gccaaccggt tcaagaagat cagcagcagc
      361 ggcgccctga ttgatggggg atctggcccc ggcggcggcg cgccaatgtc gaatctgagc
      421 aaaggcacgg gcagccggaa ggacaccaag atgcggatcc gggcctttcc gatgaccatg
      481 gatgaaaaat atgtaaacag catttgggac cttctgaaaa atgcaattca agaaatccag
      541 cgtaagaata acagtggtct tagttttgag gagctctata gaaatgcata tacaatggtt
      601 ttgcataaac atggagaaaa gctctacact ggactaagag aagttgttac cgaacatctc
      661 ataaataagg tgcgagaaga tgtactaaat tcattgaata acaactttct tcaaacgcta
      721 aatcaagctt ggaatgatca tcaaacagct atggtgatga ttagagacat actaatgtac
      781 atggaccgtg tgtatgtaca acaaaataat gtggagaacg
//
