LOCUS       sequence-488089-        2427 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Strong, constitutive promoter, including ribosome 
            binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_FULLPRM_Pem7
FEATURES             Location/Qualifiers
     source          1..2427
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        12..59
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(132..150)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(133..150)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(166..182)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(166..182)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(297..885)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(377..396)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1000..1102
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1103..1762
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1169..1188)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1875..2330
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1962..1981)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2172..2193
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2349..2366
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2350..2366
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2373..2392
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2373..2391
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga ggttgacaat taatcatcgg catagtatat cggcatagta taatacgaca
       61 aggtgaggaa ctaaacccag gaggaaaaca aaatgcgaga ccactcatcg cccaatcact
      121 agtgaattcg ttctatagtg tcacctaaat accatagagc cgttggtcat agctgtttcc
      181 tgtccaggca tcatgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
      241 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
      301 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
      361 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
      421 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
      481 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
      541 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
      601 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
      661 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
      721 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
      781 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
      841 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
      901 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
      961 agattatcaa aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc
     1021 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
     1081 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     1141 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     1201 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     1261 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     1321 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     1381 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     1441 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     1501 tttccctaaa gggtttattg agaatatgtt tttcgtgtca gccaatccct gggtgagttt
     1561 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     1621 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     1681 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     1741 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
     1801 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg
     1861 tcaaacatga tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac
     1921 gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc
     1981 ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt
     2041 agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg
     2101 ttcacgtagt gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac
     2161 gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta
     2221 ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat
     2281 ttaacaaaaa tttaacgcga attttaacaa aatattaacg cttacaattt taacattgta
     2341 gaaacgcatg taaaacgacg gccagtgaat tgtaatacga ctcactatag ggtgaattcc
     2401 gcttaggacc ttagatattg atctcaa
//
