LOCUS       sequence-48809-e         328 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Glover CP, Heywood DJ, Bienemann AS, Deuschle U, Kew JN, Uney JB
  TITLE     Adenoviral expression of CREB protects neurons from apoptotic and 
            excitotoxic stress.
  JOURNAL   Neuroreport. 2004 May 19;15(7):1171-5.
  PUBMED    15129168
REFERENCE   2  (bases 1 to 328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Neuroreport."; date: "2004-05-19"; volume: "15(7)"; pages: "1171-5"
COMMENT     Sequence Label: pXCX.CMV.ACREB.WPRE
FEATURES             Location/Qualifiers
     source          1..328
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        23..41
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             65..88
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             92..124
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
ORIGIN
        1 cagctcttaa ggctagagta cttaatacga ctcactatag gctagcctcg agaattccac
       61 catggactac aaggacgacg atgacaagca tatggctagc atgactggtg gacagcaaat
      121 gggtcgggat cctgacctgg aacaacgtgc tgaggaactg gcccgtgaaa acgaagagct
      181 ggaaaaagag gccgaagagc tggagcagga actggcagaa ctcgagaaca gagtggcagt
      241 gcttgaaaac caaaacaaaa cattgattga ggagctaaaa gcacttaagg acctttactg
      301 ccacaagtca gattaagaat tccgcccc
//
