LOCUS       sequence-488091-        2477 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 IPTG/Lactose-Inducible promoter, including ribosome 
            binding site. Lacks repressor, LacI..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_FULLPRM_Plac
FEATURES             Location/Qualifiers
     source          1..2477
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    32..53
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(68..73,74..91,92..98)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    106..122
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     111..133
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     promoter        complement(182..200)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(183..200)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(216..232)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(216..232)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(347..935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(427..446)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        1050..1152
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             1153..1812
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(1219..1238)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1925..2380
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2012..2031)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2222..2243
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2399..2416
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     2400..2416
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2423..2442
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2423..2441
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
       61 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
      121 aatttcacac aggaaacagc taatgcgaga ccactcatcg cccaatcact agtgaattcg
      181 ttctatagtg tcacctaaat accatagagc cgttggtcat agctgtttcc tgtccaggca
      241 tcatgacacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
      301 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
      361 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
      421 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
      481 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
      541 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
      601 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
      661 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
      721 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
      781 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
      841 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
      901 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
      961 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     1021 aaaggatctt cacctagatc cttttgtgat gatcggcacg taagaggttc caactttcac
     1081 cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt ttcaggagct
     1141 aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat atcccaatgg
     1201 catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta taaccagacc
     1261 gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca caagttttat
     1321 ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt ccgtatggca
     1381 atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac cgttttccat
     1441 gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt ccggcagttt
     1501 ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta tttccctaaa
     1561 gggtttattg agaatatgtt tttcgtgtca gccaatccct gggtgagttt caccagtttt
     1621 gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat gggcaaatat
     1681 tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca tgccgtttgt
     1741 gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga tgagtggcag
     1801 ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg ttgctacgcc
     1861 tgaataagtg ataataagcg gatgaatggc agaaattcga tgataagctg tcaaacatga
     1921 tgttacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     1981 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     2041 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     2101 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     2161 gggccagcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     2221 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     2281 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     2341 tttaacgcga attttaacaa aatattaacg cttacaattt taacattgta gaaacgcatg
     2401 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggtgaattcc gcttaggacc
     2461 ttagatattg atctcaa
//
