LOCUS       sequence-488092-        3649 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GoldenBraid2.0 Arabinose-Inducible promoter, including ribosome 
            binding site..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Cooke MB, Welch KT, Deus Ramirez L, Wen AX, Marciano DC,, Herman C
  TITLE     GoldenBraid2.0 E. coli: A comprehensive and characterized toolkit 
            for enterics.
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUPD3_FULLPRM_araC_Para
FEATURES             Location/Qualifiers
     source          1..3649
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(97..975)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        1002..1286
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1209..1228
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     promoter        complement(1354..1372)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(1355..1372)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1388..1404)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1388..1404)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(1519..2107)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1599..1618)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        2222..2324
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             2325..2984
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(2391..2410)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3097..3552
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3184..3203)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3394..3415
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     3572..3588
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3595..3614
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3595..3613
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ggtctcggga gaatgtgcct gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta
       61 ctccgtcaag ccgtcaattg tctgattcgt taccaattat gacaacttga cggctacatc
      121 attcactttt tcttcacaac cggcacgaaa ctcgctcggg ctggccccgg tgcatttttt
      181 aaatactcgc gagaaataga gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat
      241 aggcatccgg gtagtgctca aaagcagctt cgcctgacta atgcgttggt cctcgcgcca
      301 gcttaagacg ctaatcccta actgctggcg gaaaagatgt gacagacgcg acggcgacaa
      361 gcaaacatgc tgtgcgacgc tggcgatatc aaaattgctg tctgccaggt gatcgctgat
      421 gtactgacaa gcctcgcgta cccgattatc catcggtgga tggagcgact cgttaatcgc
      481 ttccatgcgc cgcagtaaca attgctcaag cagatttatc gccagcagct ccgaatagcg
      541 cccttcccct tgcccggcgt taatgatttg cccaaacagg tcgctgaaat gcggctggtg
      601 cgcttcatcc gggcgaaaga aacccgtatt ggcaaatatt gacggccagt taagccattc
      661 atgccagtag gcgcgcggac gaaagtaaac ccactggtga taccattcgc gagcctccgg
      721 atgacgaccg tagtgatgaa tctctcctgg cgggaacagc aaaatatcac ccggtcggca
      781 gacaaattct cgtccctgat ttttcaccac cccctgaccg cgaatggtga gattgagaat
      841 ataacctttc attcccagcg gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat
      901 cggcgttaaa cccgccacca gatgggcgtt aaacgagtat cccggcagca ggggatcatt
      961 ttgcgcttca gccatacttt tcatactccc accattcaga gaagaaacca attgtccata
     1021 ttgcatcaga cattgccgtc actgcgtctt ttactggctc ttctcgctaa cccaaccggt
     1081 aaccccgctt attaaaagca ttctgtaaca aagcgggacc aaagccatga caaaaacgcg
     1141 taacaaaagt gtctataatc acggcagaaa agtccacatt gattatttgc acggcgtcac
     1201 actttgctat gccatagcat ttttatccat aagattagcg gatcctacct gacgcttttt
     1261 atcgcaactc tctactgttt ctccataccc gtttttttgg atggagtgaa acgaatgcga
     1321 gaccactcat cgcccaatca ctagtgaatt cgttctatag tgtcacctaa ataccataga
     1381 gccgttggtc atagctgttt cctgtccagg catcatgaca cggttatcca cagaatcagg
     1441 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     1501 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     1561 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     1621 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     1681 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     1741 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     1801 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     1861 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     1921 gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc
     1981 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     2041 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     2101 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     2161 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttgtg
     2221 atgatcggca cgtaagaggt tccaactttc accataatga aataagatca ctaccgggcg
     2281 tattttttga gttatcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca
     2341 ctggatatac caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc
     2401 agtcagttgc tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa
     2461 agaccgtaaa gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc
     2521 tgatgaatgc tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg
     2581 atagtgttca cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct
     2641 ggagtgaata ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt
     2701 gttacggtga aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtgt
     2761 cagccaatcc ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact
     2821 tcttcgcccc cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc
     2881 cgctggcgat tcaggttcat catgccgttt gtgatggctt ccatgtcggc agaatgctta
     2941 atgaattaca acagtactgc gatgagtggc agggcggggc gtaatttttt taaggcagtt
     3001 attggtgccc ttaaacgcct ggttgctacg cctgaataag tgataataag cggatgaatg
     3061 gcagaaattc gatgataagc tgtcaaacat gatgttacgc gccctgtagc ggcgcattaa
     3121 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     3181 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     3241 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     3301 aaaaacttga ttagggtgat ggttcacgta gtgggccagc gccctgatag acggtttttc
     3361 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     3421 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     3481 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     3541 cgcttacaat tttaacattg tagaaacgca tgtaaaacga cggccagtga attgtaatac
     3601 gactcactat agggtgaatt ccgcttagga ccttagatat tgatctcaa
//
